##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549976_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	789963
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.124838758270954	33.0	31.0	34.0	30.0	34.0
2	32.290364232248855	34.0	31.0	34.0	30.0	34.0
3	32.28903758783639	34.0	31.0	34.0	30.0	34.0
4	35.87405612667935	37.0	35.0	37.0	35.0	37.0
5	35.738592820170055	37.0	35.0	37.0	35.0	37.0
6	35.68053313889385	37.0	35.0	37.0	33.0	37.0
7	35.93836167010354	37.0	35.0	37.0	35.0	37.0
8	35.91564288454016	37.0	35.0	37.0	35.0	37.0
9	37.42525789182531	39.0	37.0	39.0	35.0	39.0
10	37.25452078135305	39.0	37.0	39.0	34.0	39.0
11	37.15004753387184	39.0	37.0	39.0	34.0	39.0
12	37.007004125509674	39.0	37.0	39.0	33.0	39.0
13	36.89302283777848	39.0	37.0	39.0	33.0	39.0
14	37.89991429978366	40.0	37.0	41.0	33.0	41.0
15	38.00500276595233	40.0	37.0	41.0	33.0	41.0
16	38.05178976736885	40.0	37.0	41.0	33.0	41.0
17	38.088763650955805	40.0	37.0	41.0	34.0	41.0
18	38.05926480100967	40.0	37.0	41.0	34.0	41.0
19	38.008690280430855	40.0	36.0	41.0	34.0	41.0
20	37.95819551042264	40.0	36.0	41.0	34.0	41.0
21	37.92972329083767	40.0	36.0	41.0	33.0	41.0
22	37.79210925068642	39.0	36.0	41.0	33.0	41.0
23	37.53247810340484	39.0	35.0	41.0	33.0	41.0
24	37.306064967599745	39.0	35.0	41.0	32.0	41.0
25	37.330162298740575	39.0	35.0	41.0	32.0	41.0
26	37.42166278673811	39.0	35.0	41.0	33.0	41.0
27	37.46614968042807	39.0	35.0	41.0	33.0	41.0
28	37.4697713690388	39.0	36.0	41.0	33.0	41.0
29	37.46303307876445	39.0	36.0	41.0	33.0	41.0
30	37.3418172749863	39.0	35.0	41.0	33.0	41.0
31	37.187972094895585	39.0	35.0	41.0	32.0	41.0
32	37.10350991122369	39.0	35.0	41.0	32.0	41.0
33	37.0458705027957	39.0	35.0	41.0	32.0	41.0
34	37.02266688439838	39.0	35.0	41.0	32.0	41.0
35	36.946970934081726	39.0	35.0	41.0	31.0	41.0
36	36.82572981266211	39.0	35.0	41.0	31.0	41.0
37	36.73433945640492	39.0	35.0	41.0	31.0	41.0
38	36.65396860359283	39.0	35.0	41.0	31.0	41.0
39	36.63248152128644	39.0	35.0	41.0	31.0	41.0
40	36.555677924155944	39.0	35.0	40.0	31.0	41.0
41	36.47005619250522	39.0	35.0	40.0	31.0	41.0
42	36.42034373761809	39.0	35.0	40.0	31.0	41.0
43	36.283451250248426	39.0	35.0	40.0	30.0	41.0
44	36.163653234391994	38.0	35.0	40.0	30.0	41.0
45	36.089923198934635	38.0	35.0	40.0	30.0	41.0
46	35.93114361052353	38.0	35.0	40.0	29.0	41.0
47	35.79230546240773	38.0	35.0	40.0	29.0	41.0
48	35.75767346065575	38.0	35.0	40.0	29.0	41.0
49	35.75106935388113	38.0	35.0	40.0	29.0	41.0
50	35.66546534457943	38.0	35.0	40.0	29.0	41.0
51	34.54345330097739	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	4.0
10	15.0
11	7.0
12	6.0
13	13.0
14	16.0
15	23.0
16	60.0
17	112.0
18	207.0
19	398.0
20	722.0
21	1095.0
22	1689.0
23	2476.0
24	3481.0
25	4509.0
26	5582.0
27	6854.0
28	8226.0
29	10403.0
30	13170.0
31	16478.0
32	21656.0
33	30369.0
34	54275.0
35	69474.0
36	65592.0
37	100634.0
38	172825.0
39	199486.0
40	104.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.633168895252055	21.99128313604561	25.209535130126348	12.166012838575984
2	31.962762812941868	24.843821799248825	25.214218893796293	17.97919649401301
3	31.156902285296905	24.282909452721206	27.453690869066023	17.10649739291587
4	28.738307996703643	25.467395308387864	25.842096401983383	19.952200292925113
5	24.50481858011071	30.173944855645136	24.932053779733987	20.389182784510158
6	26.106792343438872	33.185604895419154	25.07332621907608	15.634276542065894
7	75.53847458678445	7.087547138283693	10.792024436587537	6.581953838344328
8	74.39019295840438	5.729382262207217	11.646115071212195	8.234309708176205
9	67.88571110292507	8.353808975863426	14.613595826639983	9.146884094571519
10	39.58881618506183	23.04361090329547	21.126052739178924	16.241520172463776
11	28.88704914027619	24.150118423267923	25.29536193467289	21.667470501782997
12	26.29021865581046	20.892370908510905	32.19935617237769	20.618054263300937
13	23.789468620682232	24.337848734687572	32.22530675487333	19.647375889756862
14	18.512132846728267	28.680836950591353	30.91233386880145	21.89469633387893
15	17.296759468481433	25.312198166243228	36.595764611760295	20.795277753515037
16	19.129756710124397	24.874835910036293	32.75267322646757	23.242734153371742
17	19.123047535137722	25.15295020146513	28.5082972240472	27.215705039349945
18	20.356396438820553	25.59638869162227	31.229437328077392	22.817777541479792
19	21.438978787614104	26.466809204988085	28.86666843890157	23.227543568496245
20	22.940821278971292	27.066963895777395	28.480827583064016	21.511387242187293
21	21.629114274972373	27.6974997563177	29.974315252739686	20.69907071597024
22	18.998864503780556	26.40769251218095	27.50052850576546	27.092914478273034
23	20.363105613807228	26.117552341059014	28.371328783753164	25.148013261380598
24	22.533587016100753	23.88175142380086	28.806539040436068	24.77812251966231
25	20.19411035706736	26.42389580271481	26.372754166967315	27.00923967325052
26	20.039419567751906	27.06747024860658	28.12511472056286	24.767995463078652
27	21.552148644936537	26.490354611545097	29.575815576172555	22.381681167345814
28	18.953545925568665	25.3161224006694	30.64903039762622	25.08130127613572
29	23.463250810481	23.283748732535575	29.137440614307252	24.115559842676177
30	23.984287871710446	23.409577410587584	30.093561344012315	22.512573373689655
31	23.052851842428062	25.053831635152534	26.957085331844656	24.93623119057475
32	25.08370645207434	23.955045995825124	25.501194359735834	25.460053192364708
33	22.272562132656844	22.538903720807177	29.289599639476787	25.89893450705919
34	20.601724384559784	20.88477561607316	30.465983849876515	28.047516149490548
35	23.057662194305305	20.871357266099803	27.53572002739369	28.535260512201205
36	22.191292503572953	22.390415753649222	30.301419180391992	25.116872562385833
37	21.143395323578446	23.04158549197874	31.21563921348215	24.599379970960666
38	21.130230150019685	24.736474999462	27.44166498937292	26.691629861145394
39	23.28805273158363	21.276059764824428	28.99009194101496	26.44579556257698
40	22.829423656551004	19.8414356115413	29.99469595411431	27.334444777793394
41	19.40559241382191	21.517843240759376	26.38756498722092	32.68899935819779
42	21.663926031978715	20.27120257531049	28.419812067147447	29.64505932556335
43	23.28931861365659	20.101194612912252	30.472186672034006	26.137300101397155
44	21.822161291098443	20.79401187144208	29.57239769457557	27.81142914288391
45	22.90613611017225	19.666743885473117	28.80008304186399	28.62703696249065
46	23.547431968332695	20.299305157330153	30.4033226872651	25.749940187072053
47	19.440150994413663	20.377283493024358	32.568487384852205	27.614078127709778
48	19.3578686596714	19.072285664012114	32.08479384477501	29.485051831541476
49	22.209774381838137	17.88792639655275	33.20649194962296	26.695807271986155
50	20.861483385930736	18.02603413071245	31.874404244249416	29.238078239107402
51	19.762824334810617	18.310351244298783	28.63058143229493	33.29624298859567
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	664.0
1	1093.5
2	1523.0
3	2062.5
4	2602.0
5	1946.5
6	1291.0
7	1316.5
8	1342.0
9	1436.0
10	1530.0
11	1578.0
12	1626.0
13	1781.5
14	1937.0
15	2022.0
16	2107.0
17	2158.5
18	2210.0
19	2399.0
20	2588.0
21	2531.5
22	2475.0
23	2744.0
24	3013.0
25	3677.5
26	4925.5
27	5509.0
28	6676.5
29	7844.0
30	9168.0
31	10492.0
32	11396.0
33	12300.0
34	12855.5
35	13411.0
36	14859.0
37	16307.0
38	17489.5
39	18672.0
40	21872.5
41	25073.0
42	29226.0
43	33379.0
44	38805.5
45	44232.0
46	50664.5
47	57097.0
48	67001.0
49	76905.0
50	91426.5
51	105948.0
52	103170.0
53	100392.0
54	81835.5
55	63279.0
56	51792.0
57	40305.0
58	35574.0
59	30843.0
60	28689.5
61	26536.0
62	24358.5
63	22181.0
64	19733.0
65	17285.0
66	14580.0
67	11875.0
68	9687.0
69	7499.0
70	6354.0
71	5209.0
72	4348.0
73	3487.0
74	2737.5
75	1611.0
76	1234.0
77	931.0
78	628.0
79	509.5
80	391.0
81	340.0
82	289.0
83	160.5
84	32.0
85	49.0
86	66.0
87	43.0
88	20.0
89	12.0
90	4.0
91	2.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	789963.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.955727544882127
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.7143320929886	21.27102142699106
2	12.902462993743082	7.988102581844703
3	5.287262051297385	4.910131305550694
4	2.6825559149957576	3.3216188011408265
5	1.6416445271738527	2.540915035437031
6	1.0478839048105104	1.946280519358882
7	0.7325473721153881	1.5873575805446338
8	0.564217505685468	1.3972610705641866
9	0.458403137289318	1.2771182361282591
>10	5.639320028888821	39.99751602995178
>50	0.24258527838403018	4.650457343339279
>100	0.0764536220768408	4.4325950550468685
>500	0.006198942330554659	1.2322074394704445
>1k	0.003306102576295818	1.9512936123590834
>5k	8.265256440739545E-4	1.496123962272306
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC	5955	0.7538327744463981	TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCT	5740	0.7266163098778043	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC	4944	0.6258520968703597	TruSeq Adapter, Index 13 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC	2006	0.2539359438353442	TruSeq Adapter, Index 13 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC	1694	0.21444042315905934	No Hit
CGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG	1496	0.18937595811449398	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCC	1476	0.18684419396857826	No Hit
GCCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG	1229	0.15557690676651945	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1212	0.1534249072424911	No Hit
GGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG	1196	0.15139949592575855	No Hit
ACCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG	950	0.1202587969309955	No Hit
AGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG	832	0.1053213884700929	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTC	817	0.10342256536065614	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	802	0.10152374225121936	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2658820729578475E-4	0.0	0.0	0.7840873559900907	0.0
2	1.2658820729578475E-4	0.0	0.0	2.7877255010677717	0.0
3	1.2658820729578475E-4	0.0	0.0	3.9425897162272157	0.0
4	1.2658820729578475E-4	0.0	0.0	5.138088745928607	0.0
5	1.2658820729578475E-4	0.0	0.0	7.969993531342607	0.0
6	1.2658820729578475E-4	0.0	0.0	9.528420951361014	0.0
7	1.2658820729578475E-4	0.0	0.0	11.199005523043484	0.0
8	1.2658820729578475E-4	0.0	0.0	13.690894383661007	0.0
9	1.2658820729578475E-4	0.0	0.0	14.68182687037241	0.0
10	1.2658820729578475E-4	0.0	0.0	16.57470033406628	0.0
11	1.2658820729578475E-4	0.0	0.0	19.562814967283277	0.0
12	1.2658820729578475E-4	0.0	0.0	21.818743409501458	0.0
13	1.2658820729578475E-4	0.0	0.0	22.73195073693325	0.0
14	1.2658820729578475E-4	0.0	0.0	23.115259828624886	0.0
15	1.2658820729578475E-4	0.0	0.0	23.79681073670539	0.0
16	1.2658820729578475E-4	0.0	0.0	25.14851961420978	0.0
17	1.2658820729578475E-4	0.0	0.0	26.757455728939203	0.0
18	5.06352829183139E-4	0.0	0.0	28.616023788455916	0.0
19	5.06352829183139E-4	0.0	0.0	29.593537925193964	0.0
20	5.06352829183139E-4	0.0	0.0	30.542189950668575	0.0
21	6.329410364789237E-4	0.0	0.0	31.635279120667676	0.0
22	6.329410364789237E-4	0.0	0.0	32.73495087744616	0.0
23	6.329410364789237E-4	0.0	0.0	33.767151119735985	0.0
24	6.329410364789237E-4	0.0	0.0	34.58680976197619	0.0
25	6.329410364789237E-4	0.0	0.0	35.288488195016726	0.0
26	6.329410364789237E-4	0.0	0.0	35.93535393429819	0.0
27	6.329410364789237E-4	0.0	0.0	36.57664979245863	0.0
28	6.329410364789237E-4	0.0	0.0	37.20389435960925	0.0
29	6.329410364789237E-4	0.0	0.0	37.83924057202679	0.0
30	6.329410364789237E-4	0.0	0.0	38.61421357709158	0.0
31	8.861174510704932E-4	0.0	0.0	39.24373673197352	0.0
32	0.001012705658366278	0.0	0.0	39.89212152974253	0.0
33	0.0011392938656620626	0.0	0.0	40.496580219579904	0.0
34	0.0011392938656620626	0.0	0.0	41.07964550238429	0.0
35	0.0011392938656620626	0.0	0.0	41.68106607524656	0.0
36	0.0011392938656620626	0.0	0.0	42.261979358526915	0.0
37	0.0011392938656620626	0.0	0.0	42.871248400241534	0.0
38	0.0012658820729578473	0.0	0.0	43.46342803397121	0.0
39	0.0012658820729578473	0.0	0.0	44.0943436591334	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGCTAT	60	0.0	45.000004	24
AGCGTGC	20	7.031946E-4	45.0	12
TCGCACT	20	7.031946E-4	45.0	19
CGACGGT	25	3.8896935E-5	45.0	28
GACACGA	20	7.031946E-4	45.0	37
ATGTGCG	20	7.031946E-4	45.0	1
CGCGACA	20	7.031946E-4	45.0	29
CGGTCTA	25	3.8896935E-5	45.0	31
CGGTAGT	50	2.1827873E-11	45.0	12
TCGACGC	20	7.031946E-4	45.0	16
TTCGATA	20	7.031946E-4	45.0	26
ATACCCG	25	3.8896935E-5	45.0	41
TGCGTAG	45	3.8380676E-10	45.0	1
GTACGCG	20	7.031946E-4	45.0	1
TACCGGT	20	7.031946E-4	45.0	18
AGCACGT	20	7.031946E-4	45.0	11
ACGGGTA	110	0.0	44.999996	5
CACTAGG	80	0.0	42.1875	2
ATTGGGT	65	0.0	41.538464	4
CGAAAGG	120	0.0	41.250004	2
>>END_MODULE