FastQCFastQC Report
Sat 18 Jun 2016
SRR3549975_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549975_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2099360
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC78680.3747808856032315No Hit
CTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCT71440.340294184894444No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59540.2836102431217133No Hit
CCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC58250.2774655132992912No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCC33950.16171595152808474No Hit
TCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC30120.14347229631887815No Hit
ACTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC22080.10517491044889872No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACCG207.034621E-445.01
TTAACGG1200.043.1250042
CGGTCTA556.184564E-1140.90909231
GTTAACG900.040.01
TATTGCG1550.039.1935461
CGTTTTT32250.038.8604661
TGCGACG700.038.5714261
CACGACC6400.037.9687527
AACACGT6150.037.31707441
TCAAGCG6850.037.11678717
GCGAGAC6700.036.940321
CGAGACA6700.036.60447722
CGTAAGG2400.036.5625042
ACACGAC6650.036.5413526
GGTTACG1050.036.4285741
TTAGCGA250.002107504336.01
CGAATCG250.002107504336.02
TATCGTG250.002107504336.01
ACGACCA6500.036.028
ATAGGGA15350.035.9120524
Produced by FastQC (version 0.11.5)