##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549975_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2099360
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.27892405304474	33.0	31.0	34.0	30.0	34.0
2	32.43873466199223	34.0	31.0	34.0	30.0	34.0
3	32.441913726087954	34.0	31.0	34.0	30.0	34.0
4	35.98948060361253	37.0	35.0	37.0	35.0	37.0
5	35.87320230927521	37.0	35.0	37.0	35.0	37.0
6	35.8297033381602	37.0	35.0	37.0	35.0	37.0
7	36.068313676549046	37.0	35.0	37.0	35.0	37.0
8	36.05652579833854	37.0	35.0	37.0	35.0	37.0
9	37.6199622742169	39.0	38.0	39.0	35.0	39.0
10	37.362196097858394	39.0	37.0	39.0	34.0	39.0
11	37.27902979955796	39.0	37.0	39.0	34.0	39.0
12	37.094946078804966	39.0	37.0	39.0	34.0	39.0
13	36.987580024388386	39.0	37.0	39.0	33.0	39.0
14	37.89095057541346	40.0	37.0	41.0	33.0	41.0
15	38.03233175825013	40.0	37.0	41.0	33.0	41.0
16	38.13923195640576	40.0	37.0	41.0	33.0	41.0
17	38.209065619998476	40.0	37.0	41.0	33.0	41.0
18	38.19284115158906	40.0	37.0	41.0	34.0	41.0
19	38.15414173843457	40.0	37.0	41.0	34.0	41.0
20	38.07978717323375	40.0	37.0	41.0	33.0	41.0
21	38.06552663668928	40.0	37.0	41.0	34.0	41.0
22	38.025481575337245	40.0	36.0	41.0	34.0	41.0
23	37.784337131316214	40.0	36.0	41.0	33.0	41.0
24	37.568611862662905	39.0	35.0	41.0	33.0	41.0
25	37.5741506935447	39.0	35.0	41.0	33.0	41.0
26	37.67219771739959	39.0	36.0	41.0	33.0	41.0
27	37.766534086578766	40.0	36.0	41.0	33.0	41.0
28	37.7370874933313	40.0	36.0	41.0	33.0	41.0
29	37.70258459721058	40.0	36.0	41.0	33.0	41.0
30	37.627365006478165	40.0	36.0	41.0	33.0	41.0
31	37.450334387622895	40.0	35.0	41.0	33.0	41.0
32	37.3559556245713	39.0	35.0	41.0	33.0	41.0
33	37.319414488224986	39.0	35.0	41.0	33.0	41.0
34	37.19820421461779	39.0	35.0	41.0	32.0	41.0
35	37.15980822726927	39.0	35.0	41.0	32.0	41.0
36	37.08404085054493	39.0	35.0	41.0	32.0	41.0
37	37.0598982547062	39.0	35.0	41.0	32.0	41.0
38	36.952935656581055	39.0	35.0	41.0	31.0	41.0
39	36.88403608718848	39.0	35.0	41.0	31.0	41.0
40	36.80451613825166	39.0	35.0	41.0	31.0	41.0
41	36.73342780656962	39.0	35.0	41.0	31.0	41.0
42	36.740237024617024	39.0	35.0	41.0	31.0	41.0
43	36.69752162563829	39.0	35.0	41.0	31.0	41.0
44	36.609310456520085	39.0	35.0	41.0	31.0	41.0
45	36.52918937199909	39.0	35.0	41.0	31.0	41.0
46	36.441617921652316	39.0	35.0	40.0	31.0	41.0
47	36.341699851383275	38.0	35.0	40.0	31.0	41.0
48	36.2830224449356	38.0	35.0	40.0	31.0	41.0
49	36.27146273149912	38.0	35.0	40.0	31.0	41.0
50	36.17924224525569	38.0	35.0	40.0	31.0	41.0
51	35.18117330996113	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	5.0
9	8.0
10	15.0
11	16.0
12	15.0
13	27.0
14	43.0
15	74.0
16	127.0
17	249.0
18	478.0
19	862.0
20	1480.0
21	2315.0
22	3567.0
23	5242.0
24	7387.0
25	10644.0
26	14159.0
27	17094.0
28	20216.0
29	24338.0
30	30835.0
31	39903.0
32	54001.0
33	79673.0
34	141691.0
35	152466.0
36	160849.0
37	253413.0
38	451569.0
39	626197.0
40	400.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.896120722505906	20.958768386555903	24.953652541727003	11.191458349211187
2	31.80974201661459	24.072193430378782	25.562266595533877	18.555797957472752
3	31.92044242054721	23.730279704290833	27.21115006478165	17.138127810380308
4	28.317344333511162	25.217494855575033	26.681798262327565	19.783362548586236
5	25.21506554378477	29.029513756573433	25.287325661153876	20.46809503848792
6	26.776446155018675	33.331920204252725	24.382954805273986	15.508678835454614
7	80.20125181007545	5.538021111195793	9.057474658943677	5.203252419785078
8	80.26755773188019	4.561437771511318	9.189181464827376	5.981823031781115
9	73.73880611233899	6.75386784543861	11.996179788125904	7.511146254096486
10	39.132449889490125	27.140366587912506	18.42704443258898	15.300139090008383
11	30.595229022178188	23.594285877600793	25.869931788735617	19.940553311485402
12	27.133650255315906	21.46173119426873	30.459425729746208	20.945192820669156
13	25.58484490511394	22.566877524578917	32.5473001295633	19.300977440743846
14	20.65462807712827	25.19996570383355	32.769177273073694	21.376228945964485
15	19.79760498437619	23.88932817620608	35.621713284048475	20.691353555369254
16	23.86927444554531	24.617788278332444	30.00652579833854	21.506411477783704
17	23.656114244341133	24.294356375276273	28.213503162868687	23.836026217513908
18	24.59158981784925	23.386222467799712	30.30428321012118	21.71790450422986
19	23.75009526712903	25.765995350964104	27.758745522444933	22.72516385946193
20	23.50930759850621	27.68691410715647	27.74731346696136	21.05646482737596
21	22.096496074994285	26.842894977516956	30.64329128877372	20.417317658715035
22	20.933093895282372	23.38865177958997	30.46942877829434	25.208825546833317
23	20.32528961207225	25.964246246475113	30.255554073622438	23.454910067830195
24	21.376562380916088	25.605422604984373	29.297928892614895	23.720086121484645
25	22.46779971038793	26.726383278713513	26.619969895587225	24.185847115311333
26	20.58808398750095	26.26476640499962	27.282886212941087	25.86426339455834
27	21.595152808474964	24.887060818535172	28.64625409648655	24.871532276503316
28	18.510022101973934	26.701613825165765	27.76179407057389	27.02657000228641
29	20.33276808170109	27.492473896806647	26.49497942230013	25.679778599192133
30	22.91598391890862	26.340360871884766	26.581005639814038	24.162649569392578
31	21.885955719838428	28.960730889413917	25.00847877448365	24.144834616264006
32	21.823508116759392	28.216885145949245	24.305359728679214	25.654247008612145
33	21.51288964255773	27.59812514290069	24.773359500038104	26.115625714503466
34	18.362167517719683	29.20699641795595	27.670575794527856	24.76026026979651
35	18.710988110662296	28.797252495998784	25.215398978736374	27.276360414602546
36	20.217018519929884	30.51210845209969	25.313285953814496	23.957587074155935
37	19.11611157686152	30.47862205624571	25.927663668927675	24.477602697965096
38	19.711197698346165	28.31720143281762	25.735366968981022	26.236233899855193
39	20.428130477859916	26.493216980413077	26.99279780504535	26.085854736681657
40	21.82336521606585	25.96667555826538	28.23455719838427	23.975402027284506
41	18.682598506211416	26.19298262327566	26.733909381906866	28.390509488606053
42	20.02214960749943	25.08126286106242	29.51004115539974	25.38654637603841
43	20.652770368112186	23.87327566496456	29.874771358890328	25.599182608032923
44	20.96486548281381	25.095648197545916	27.10387927749409	26.835607042146176
45	20.39821659934456	24.033610243121714	26.6902294032467	28.87794375428702
46	21.13606051368036	25.426415669537384	27.244731727764655	26.19279208901761
47	18.00610662297081	26.379134593399893	30.234976373751998	25.379782409877294
48	18.673357594695524	26.133964636841707	28.677120646292202	26.51555712217057
49	21.37842008993217	23.95725363920433	29.834997332520384	24.829328938343114
50	19.170032771892387	24.228336254858622	28.84855384498133	27.753077128267662
51	18.839455834158983	24.015890557122173	26.780590275131466	30.364063333587378
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	683.0
1	1800.0
2	2917.0
3	6338.0
4	9759.0
5	6515.0
6	3271.0
7	3163.5
8	3056.0
9	3101.5
10	3147.0
11	3247.5
12	3348.0
13	3565.0
14	3782.0
15	3699.0
16	3616.0
17	3587.5
18	3559.0
19	3947.5
20	4336.0
21	4970.0
22	5604.0
23	6744.5
24	7885.0
25	9242.5
26	12733.0
27	14866.0
28	18234.5
29	21603.0
30	24243.5
31	26884.0
32	30570.0
33	34256.0
34	39169.0
35	44082.0
36	49984.0
37	55886.0
38	61688.5
39	67491.0
40	79700.5
41	91910.0
42	103798.0
43	115686.0
44	147770.0
45	179854.0
46	208007.0
47	236160.0
48	235570.0
49	234980.0
50	208205.0
51	181430.0
52	162701.0
53	143972.0
54	131948.0
55	119924.0
56	112716.0
57	105508.0
58	98370.5
59	91233.0
60	82416.0
61	73599.0
62	66292.5
63	58986.0
64	51562.0
65	44138.0
66	38202.5
67	32267.0
68	26824.0
69	21381.0
70	17916.0
71	14451.0
72	12007.0
73	9563.0
74	7909.5
75	5044.0
76	3832.0
77	2856.0
78	1880.0
79	1420.5
80	961.0
81	692.5
82	424.0
83	275.0
84	126.0
85	122.0
86	118.0
87	89.5
88	61.0
89	40.0
90	19.0
91	12.5
92	6.0
93	4.0
94	2.0
95	1.0
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2099360.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.990108775025945
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.19171641869293	19.36683668889964
2	12.378247167732718	6.929369693379912
3	4.902495782487919	4.116641706628284
4	2.4359199754785834	2.727266603236164
5	1.4632364693471096	2.047807397030526
6	0.9867421205475047	1.6571411572214652
7	0.730659122704658	1.4315859825377888
8	0.5886799436484407	1.31817725251168
9	0.46868770188026554	1.1806757781431008
>10	6.306018121956542	43.448160344302735
>50	0.4607872906474196	7.803436782196475
>100	0.07554734513687124	3.814937306071774
>500	0.007104063527670861	1.263044281452993
>1k	0.003465396842766274	1.5955979288024984
>5k	6.930793685532547E-4	1.2993210975850376
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC	7868	0.3747808856032315	No Hit
CTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCT	7144	0.340294184894444	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5954	0.2836102431217133	No Hit
CCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC	5825	0.2774655132992912	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCC	3395	0.16171595152808474	No Hit
TCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC	3012	0.14347229631887815	No Hit
ACTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC	2208	0.10517491044889872	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4290069354469935E-4	0.0	0.0	0.3614911211035744	0.0
2	1.4290069354469935E-4	0.0	0.0	1.3215932474658945	0.0
3	1.4290069354469935E-4	0.0	0.0	1.9483080557884307	0.0
4	1.4290069354469935E-4	0.0	0.0	2.7108261565429466	0.0
5	1.4290069354469935E-4	0.0	0.0	4.549719914640653	0.0
6	1.4290069354469935E-4	0.0	0.0	5.719076289916927	0.0
7	1.4290069354469935E-4	0.0	0.0	6.895148997789803	0.0
8	1.4290069354469935E-4	0.0	0.0	8.813543175062875	0.0
9	1.4290069354469935E-4	0.0	0.0	9.555197774559867	0.0
10	1.9053425805959912E-4	0.0	0.0	11.046461778827833	0.0
11	1.9053425805959912E-4	0.0	0.0	13.09884917308132	0.0
12	1.9053425805959912E-4	0.0	0.0	14.658657876686227	0.0
13	1.9053425805959912E-4	0.0	0.0	15.291660315524732	0.0
14	1.9053425805959912E-4	0.0	0.0	15.58513070650103	0.0
15	1.9053425805959912E-4	0.0	0.0	16.083473058455912	0.0
16	1.9053425805959912E-4	0.0	0.0	16.965265604755736	0.0
17	1.9053425805959912E-4	0.0	0.0	18.031638213550796	0.0
18	2.381678225744989E-4	0.0	0.0	19.19165841018215	0.0
19	2.381678225744989E-4	0.0	0.0	19.929168889566345	0.0
20	2.381678225744989E-4	0.0	0.0	20.695211874094962	0.0
21	2.381678225744989E-4	0.0	0.0	21.6134917308132	0.0
22	2.381678225744989E-4	0.0	0.0	22.573736757869064	0.0
23	2.381678225744989E-4	0.0	0.0	23.479107918603766	0.0
24	2.381678225744989E-4	0.0	0.0	24.19970848258517	0.0
25	2.381678225744989E-4	0.0	0.0	24.82685199298834	0.0
26	2.381678225744989E-4	0.0	0.0	25.433322536392044	0.0
27	2.381678225744989E-4	0.0	0.0	26.138537459035135	0.0
28	2.381678225744989E-4	0.0	0.0	26.778732566115387	0.0
29	2.381678225744989E-4	0.0	0.0	27.482661382516575	0.0
30	2.381678225744989E-4	0.0	0.0	28.25380115844829	0.0
31	2.381678225744989E-4	0.0	0.0	28.874180702690342	0.0
32	2.381678225744989E-4	0.0	0.0	29.48350925996494	0.0
33	2.858013870893987E-4	0.0	0.0	30.09002743693316	0.0
34	2.858013870893987E-4	0.0	0.0	30.728793537077966	0.0
35	2.858013870893987E-4	0.0	0.0	31.40309427635089	0.0
36	2.858013870893987E-4	0.0	0.0	31.998132764271016	0.0
37	3.3343495160429847E-4	0.0	0.0	32.635088788964254	0.0
38	3.8106851611919825E-4	0.0	0.0	33.34449546528466	0.0
39	3.8106851611919825E-4	0.0	0.0	34.447450651627165	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTACCG	20	7.034621E-4	45.0	1
TTAACGG	120	0.0	43.125004	2
CGGTCTA	55	6.184564E-11	40.909092	31
GTTAACG	90	0.0	40.0	1
TATTGCG	155	0.0	39.193546	1
CGTTTTT	3225	0.0	38.860466	1
TGCGACG	70	0.0	38.571426	1
CACGACC	640	0.0	37.96875	27
AACACGT	615	0.0	37.317074	41
TCAAGCG	685	0.0	37.116787	17
GCGAGAC	670	0.0	36.9403	21
CGAGACA	670	0.0	36.604477	22
CGTAAGG	240	0.0	36.562504	2
ACACGAC	665	0.0	36.54135	26
GGTTACG	105	0.0	36.428574	1
TTAGCGA	25	0.0021075043	36.0	1
CGAATCG	25	0.0021075043	36.0	2
TATCGTG	25	0.0021075043	36.0	1
ACGACCA	650	0.0	36.0	28
ATAGGGA	1535	0.0	35.912052	4
>>END_MODULE