FastQCFastQC Report
Sat 18 Jun 2016
SRR3549974_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549974_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1329757
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC69320.5212982522370628Illumina PCR Primer Index 7 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT66860.5027986316296887RNA PCR Primer, Index 16 (96% over 26bp)
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC64670.4863294571865385RNA PCR Primer, Index 16 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC25760.19371960440892586Illumina PCR Primer Index 7 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC19610.14747055289049052Illumina PCR Primer Index 7 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCC19100.14363526569140078No Hit
CGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG18380.13822074258680345RNA PCR Primer, Index 16 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16540.1243836279861659No Hit
GCCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG14730.11077211851488655Illumina PCR Primer Index 7 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG14550.10941848773873722RNA PCR Primer, Index 16 (95% over 24bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA14190.10671122618643857No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTTA207.0336857E-445.00000416
GTACCGG451.9286745E-840.02
ACGGGAT1800.038.755
CTATCGG356.249029E-638.5714262
GCGTCTA356.249029E-638.57142629
AGTACGG700.038.5714262
TGTTCGG601.5643309E-1037.5000042
ACGCTAG301.14001814E-437.5000041
TATAGCG301.14001814E-437.5000041
AATAGGT301.14001814E-437.50000435
TATTGCG900.037.51
ATAGGGA6750.037.04
CTACGAA1100.036.8181811
GCTCGAT552.750312E-936.8181822
CAACCGG800.036.5625042
ACCGCGC250.002107227536.013
GACCGAA250.002107227536.019
ACGATTG250.002107227536.01
CTTGCGG1500.036.02
TACGAAG1250.036.01
Produced by FastQC (version 0.11.5)