##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549974_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1329757
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.26070477538377	33.0	31.0	34.0	30.0	34.0
2	32.42448131500718	34.0	31.0	34.0	30.0	34.0
3	32.42699455614823	34.0	31.0	34.0	30.0	34.0
4	35.97041489535306	37.0	35.0	37.0	35.0	37.0
5	35.84961162076981	37.0	35.0	37.0	35.0	37.0
6	35.79965963706151	37.0	35.0	37.0	35.0	37.0
7	36.01825371101637	37.0	35.0	37.0	35.0	37.0
8	35.97390876678972	37.0	35.0	37.0	35.0	37.0
9	37.48501117121399	39.0	37.0	39.0	35.0	39.0
10	37.322297231749864	39.0	37.0	39.0	34.0	39.0
11	37.249171841170984	39.0	37.0	39.0	34.0	39.0
12	37.15209771409363	39.0	37.0	39.0	34.0	39.0
13	37.11655964210002	39.0	37.0	39.0	33.0	39.0
14	38.25002613259415	40.0	37.0	41.0	33.0	41.0
15	38.340627648510214	40.0	38.0	41.0	34.0	41.0
16	38.35974241910364	40.0	38.0	41.0	34.0	41.0
17	38.390830805929205	40.0	38.0	41.0	34.0	41.0
18	38.35656890695067	40.0	37.0	41.0	34.0	41.0
19	38.32222353407427	40.0	37.0	41.0	34.0	41.0
20	38.29263692539313	40.0	37.0	41.0	34.0	41.0
21	38.28132282815582	40.0	37.0	41.0	34.0	41.0
22	38.166546218594824	40.0	37.0	41.0	34.0	41.0
23	37.90656789172758	40.0	36.0	41.0	33.0	41.0
24	37.70298859114861	40.0	36.0	41.0	33.0	41.0
25	37.708339944816984	39.0	36.0	41.0	33.0	41.0
26	37.77028584921907	40.0	36.0	41.0	33.0	41.0
27	37.84894458160401	40.0	36.0	41.0	33.0	41.0
28	37.82449500171836	40.0	36.0	41.0	33.0	41.0
29	37.81205363085135	40.0	36.0	41.0	33.0	41.0
30	37.766272333967784	40.0	36.0	41.0	33.0	41.0
31	37.60311019231333	40.0	36.0	41.0	33.0	41.0
32	37.50607141003958	40.0	36.0	41.0	33.0	41.0
33	37.4844433983051	40.0	36.0	41.0	33.0	41.0
34	37.38906506978343	40.0	36.0	41.0	33.0	41.0
35	37.35956945517113	40.0	36.0	41.0	33.0	41.0
36	37.282657658504526	39.0	35.0	41.0	33.0	41.0
37	37.214281255898634	39.0	35.0	41.0	32.0	41.0
38	37.14754500258318	39.0	35.0	41.0	32.0	41.0
39	37.082962526235995	39.0	35.0	41.0	32.0	41.0
40	36.99683250398381	39.0	35.0	41.0	32.0	41.0
41	36.934134582483864	39.0	35.0	41.0	32.0	41.0
42	36.91242986500541	39.0	35.0	41.0	32.0	41.0
43	36.82730980171565	39.0	35.0	41.0	31.0	41.0
44	36.724275939137755	39.0	35.0	41.0	31.0	41.0
45	36.66231574641081	39.0	35.0	41.0	31.0	41.0
46	36.536647673221495	39.0	35.0	40.0	31.0	41.0
47	36.43138107188005	39.0	35.0	40.0	31.0	41.0
48	36.3893568524174	39.0	35.0	40.0	31.0	41.0
49	36.36983674460823	39.0	35.0	40.0	31.0	41.0
50	36.27719726235696	38.0	35.0	40.0	31.0	41.0
51	35.24889284282767	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	1.0
9	6.0
10	13.0
11	17.0
12	9.0
13	15.0
14	26.0
15	36.0
16	71.0
17	118.0
18	274.0
19	484.0
20	768.0
21	1314.0
22	2029.0
23	2960.0
24	4260.0
25	5828.0
26	7747.0
27	9647.0
28	11917.0
29	14432.0
30	18222.0
31	23671.0
32	31632.0
33	44302.0
34	79176.0
35	105168.0
36	105760.0
37	166863.0
38	295082.0
39	397688.0
40	219.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.9162298074009	21.66583819449719	24.653075712329393	11.764856285772513
2	32.42487161188097	24.564036887942684	24.571632260631077	18.43945923954527
3	32.60783737179048	23.602131818069015	25.844421198760376	17.945609611380124
4	29.185031550877337	25.94158180780398	25.786515882225096	19.08687075909358
5	25.400505505893182	30.13753640702775	23.905345111926465	20.556612975152603
6	26.72164914341493	34.42087539302294	23.827360938878307	15.030114524683832
7	77.92010119142068	6.529689259014992	9.557234893292534	5.992974656271785
8	76.47863481824123	6.077125369522402	10.35369620163684	7.090543610599531
9	69.51585891256823	8.302719970641252	13.33318794336108	8.848233173429431
10	37.96144709146107	25.22415749644484	20.219032499922918	16.595362912171172
11	29.07809471956154	24.999003577345334	25.11346057963974	20.809441123453382
12	26.380082977566577	22.159011007274263	30.823601605406097	20.63730440975306
13	23.470904834492316	23.98302847813548	32.227091115143594	20.31897557222861
14	19.206667082782793	28.64064637373595	29.73362802376675	22.419058519714504
15	18.76523304633854	26.219226520334164	33.94845825214682	21.06708218118047
16	20.08419583427649	25.477286451584764	31.88875862281605	22.5497590913227
17	20.21519721272383	25.65333365419396	28.811655061789487	25.319814071292722
18	21.055275512744057	25.904808171718592	30.186191913259343	22.85372440227801
19	22.268429495013	26.61185464712726	28.18063751497454	22.939078342885207
20	23.45090117968922	26.7790280479817	27.57616617171408	22.193904600615
21	22.96863261483113	26.84204708078243	29.072529793037376	21.116790511349066
22	20.58082792570372	26.104694316329972	26.535675315113966	26.77880244285234
23	21.573264889750536	26.290292136082005	27.679117312411215	24.457325661756247
24	22.056059866577126	24.40784293671701	28.766985246176557	24.769111950529307
25	21.125438707974464	26.650809132796443	26.36857711596931	25.85517504325978
26	20.25294847103644	27.995716510610585	27.51435036626993	24.23698465208305
27	23.247630958137464	27.049378194662633	27.390568351962052	22.31242249523785
28	19.64749950554876	26.74917296919663	28.95807279074297	24.645254734511642
29	20.889681347795122	26.55703260069321	26.415352579456247	26.13793347205542
30	23.79036169766356	26.187340995384872	25.312970715702193	24.70932659124938
31	22.19315258351714	28.11461041378237	24.839726356018428	24.852510646682063
32	21.91656069492396	27.730480080195104	23.566561409340203	26.786397815540735
33	22.529830638229388	25.319588466163367	24.13816960542415	28.012411290183092
34	19.258556262535183	26.36782509887145	27.133077697654535	27.240540940938835
35	21.237414053845928	25.060668979369915	24.189156364659105	29.51276060212505
36	21.90828850684749	26.927701828228766	24.415438309405403	26.74857135551834
37	20.964807855871413	27.34040881153474	27.346124141478477	24.348659191115367
38	19.843249556121908	25.652882443935244	25.238445821304197	29.265422178638655
39	21.403158622214434	24.331212394445	25.70259077410384	28.563038209236723
40	21.486030906398685	25.179412479122128	27.293858953177157	26.040697661302026
41	19.016632362153384	26.26682920262875	25.075483716197773	29.641054719020094
42	20.980224206377557	24.77572970099048	26.48175568919735	27.762290403434616
43	22.977205609746743	22.651582206373043	27.92600452563889	26.445207658241316
44	20.897878334161806	23.62845241649414	27.03486426467392	28.438804984670128
45	21.406617900864592	22.85605565528138	26.239230175137262	29.49809626871677
46	22.0259791826627	24.72248689046194	26.661337372166493	26.590196554708868
47	20.012829411689502	24.094176605199298	29.2397783956016	26.6532155875096
48	19.551241317022583	22.26594783859006	29.004697850810334	29.17811299357702
49	21.27794777542062	21.64342808498094	29.697456001359647	27.38116813823879
50	19.944019847235246	21.70531909213488	29.49914909265377	28.8515119679761
51	20.118563015648725	21.475126658479706	26.201478916824655	32.20483140904691
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	656.0
1	1328.5
2	2001.0
3	2846.0
4	3691.0
5	2759.0
6	1827.0
7	1812.0
8	1797.0
9	1969.5
10	2142.0
11	2146.5
12	2151.0
13	2241.5
14	2332.0
15	2316.5
16	2301.0
17	2596.0
18	2891.0
19	2732.0
20	2573.0
21	3094.5
22	3616.0
23	3980.0
24	4344.0
25	5715.5
26	8851.5
27	10616.0
28	13513.0
29	16410.0
30	18464.5
31	20519.0
32	22470.5
33	24422.0
34	26324.5
35	28227.0
36	30537.5
37	32848.0
38	36739.0
39	40630.0
40	44967.5
41	49305.0
42	53593.0
43	57881.0
44	66029.0
45	74177.0
46	83394.5
47	92612.0
48	108494.0
49	124376.0
50	141994.0
51	159612.0
52	152917.0
53	146222.0
54	121528.5
55	96835.0
56	82283.0
57	67731.0
58	62215.5
59	56700.0
60	54438.5
61	52177.0
62	48123.0
63	44069.0
64	38395.5
65	32722.0
66	27492.0
67	22262.0
68	18454.5
69	14647.0
70	12089.5
71	9532.0
72	8096.5
73	6661.0
74	5418.0
75	3123.5
76	2072.0
77	1751.0
78	1430.0
79	1142.5
80	855.0
81	641.0
82	427.0
83	280.5
84	134.0
85	83.0
86	32.0
87	23.0
88	14.0
89	10.0
90	6.0
91	4.5
92	3.0
93	2.5
94	2.0
95	3.0
96	4.0
97	2.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1329757.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.556858244414563
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.20887272357106	18.796222366547628
2	12.671937160003985	6.983975520007181
3	5.157066467231156	4.263376487835383
4	2.739044787536489	3.0191787574098257
5	1.6117535021024574	2.2207431391188073
6	1.0518755205091461	1.7391830765664191
7	0.7921693225242338	1.5280788408461963
8	0.5710036992525827	1.2588054397871795
9	0.4355684125661132	1.0802607300746154
>10	6.163842107408362	42.05142184390613
>50	0.5019460570532928	8.354391757757499
>100	0.08415893394953296	4.485475452592821
>500	0.005794549541626566	0.9983015285117947
>1k	0.00413896395830469	1.6933639302275625
>5k	8.27792791660938E-4	1.527221128810983
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	6932	0.5212982522370628	Illumina PCR Primer Index 7 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT	6686	0.5027986316296887	RNA PCR Primer, Index 16 (96% over 26bp)
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	6467	0.4863294571865385	RNA PCR Primer, Index 16 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	2576	0.19371960440892586	Illumina PCR Primer Index 7 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	1961	0.14747055289049052	Illumina PCR Primer Index 7 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCC	1910	0.14363526569140078	No Hit
CGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG	1838	0.13822074258680345	RNA PCR Primer, Index 16 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1654	0.1243836279861659	No Hit
GCCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG	1473	0.11077211851488655	Illumina PCR Primer Index 7 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG	1455	0.10941848773873722	RNA PCR Primer, Index 16 (95% over 24bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1419	0.10671122618643857	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.504034195721474E-4	0.0	0.0	0.5343833497398397	0.0
2	1.504034195721474E-4	0.0	0.0	1.9828434819294052	0.0
3	1.504034195721474E-4	0.0	0.0	2.8499943974726207	0.0
4	1.504034195721474E-4	0.0	0.0	3.705338644579423	0.0
5	1.504034195721474E-4	0.0	0.0	5.809031274135049	0.0
6	1.504034195721474E-4	0.0	0.0	7.073247217348734	0.0
7	1.504034195721474E-4	0.0	0.0	8.3483674084814	0.0
8	1.504034195721474E-4	0.0	0.0	10.41806886521372	0.0
9	1.504034195721474E-4	0.0	0.0	11.184223884514239	0.0
10	1.504034195721474E-4	0.0	0.0	12.735108745432436	0.0
11	1.504034195721474E-4	0.0	0.0	15.066587353930078	0.0
12	1.504034195721474E-4	0.0	0.0	16.78923291999967	0.0
13	1.504034195721474E-4	0.0	0.0	17.50613081939031	0.0
14	1.504034195721474E-4	0.0	0.0	17.799868697814713	0.0
15	1.504034195721474E-4	0.0	0.0	18.362227083594973	0.0
16	1.504034195721474E-4	0.0	0.0	19.514392479227407	0.0
17	1.504034195721474E-4	0.0	0.0	20.81064435080996	0.0
18	1.504034195721474E-4	0.0	0.0	22.259330088128884	0.0
19	1.504034195721474E-4	0.0	0.0	23.07128294868912	0.0
20	1.504034195721474E-4	0.0	0.0	23.86902268609979	0.0
21	1.504034195721474E-4	0.0	0.0	24.833259008976828	0.0
22	1.504034195721474E-4	0.0	0.0	25.823891131988777	0.0
23	1.504034195721474E-4	0.0	0.0	26.769101422289935	0.0
24	1.504034195721474E-4	0.0	0.0	27.604818023142574	0.0
25	1.504034195721474E-4	0.0	0.0	28.29464330701023	0.0
26	1.504034195721474E-4	0.0	0.0	28.894978556232456	0.0
27	1.504034195721474E-4	0.0	0.0	29.497344251618905	0.0
28	1.504034195721474E-4	0.0	0.0	30.101213981201077	0.0
29	1.504034195721474E-4	0.0	0.0	30.731780317757305	0.0
30	1.504034195721474E-4	0.0	0.0	31.429952991411213	0.0
31	1.504034195721474E-4	0.0	0.0	32.080898991319465	0.0
32	2.2560512935822108E-4	0.0	0.0	32.75245025970911	0.0
33	2.2560512935822108E-4	0.0	0.0	33.35910245255336	0.0
34	2.2560512935822108E-4	0.0	0.0	33.961844156488745	0.0
35	2.2560512935822108E-4	0.0	0.0	34.576618133989896	0.0
36	2.2560512935822108E-4	0.0	0.0	35.178006207149124	0.0
37	2.2560512935822108E-4	0.0	0.0	35.763902728092425	0.0
38	2.2560512935822108E-4	0.0	0.0	36.313852831757984	0.0
39	2.2560512935822108E-4	0.0	0.0	36.890349138978024	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGTTA	20	7.0336857E-4	45.000004	16
GTACCGG	45	1.9286745E-8	40.0	2
ACGGGAT	180	0.0	38.75	5
CTATCGG	35	6.249029E-6	38.571426	2
GCGTCTA	35	6.249029E-6	38.571426	29
AGTACGG	70	0.0	38.571426	2
TGTTCGG	60	1.5643309E-10	37.500004	2
ACGCTAG	30	1.14001814E-4	37.500004	1
TATAGCG	30	1.14001814E-4	37.500004	1
AATAGGT	30	1.14001814E-4	37.500004	35
TATTGCG	90	0.0	37.5	1
ATAGGGA	675	0.0	37.0	4
CTACGAA	110	0.0	36.81818	11
GCTCGAT	55	2.750312E-9	36.81818	22
CAACCGG	80	0.0	36.562504	2
ACCGCGC	25	0.0021072275	36.0	13
GACCGAA	25	0.0021072275	36.0	19
ACGATTG	25	0.0021072275	36.0	1
CTTGCGG	150	0.0	36.0	2
TACGAAG	125	0.0	36.0	1
>>END_MODULE