FastQCFastQC Report
Sat 18 Jun 2016
SRR3549973_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549973_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1558494
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC44920.2882269678291992No Hit
GCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC44880.2879703097990753No Hit
CTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCT42310.2714800313636113No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41800.26820764147953086No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCC23320.1496316315622646No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG16260.10433148924538689No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATAG406.8175723E-945.01
GTATACG406.8175723E-945.01
TATAGCG406.8175723E-945.01
CGGTCTA551.8189894E-1245.031
TGCGACG556.184564E-1140.9090921
TACGAAT2550.040.58823412
AATTGCG501.0822987E-940.5000041
GTACGAG1950.039.230771
TAACGCC356.249724E-638.57142612
GCTACGA2750.037.63636410
TCGAATA301.1401085E-437.49999622
CGACAGG1700.037.0588232
CGAATAT2800.036.96428314
TAGCACG552.750312E-936.818181
CTACGAA2950.036.6101711
CGTTCGG2850.036.3157945
TACGAGG3550.036.1267622
TAGGGTC4050.036.111115
GATCGAG504.8808943E-836.0000041
ACGTTAC250.00210733836.00000428