##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549973_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1558494
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.34635359520152	33.0	31.0	34.0	30.0	34.0
2	32.509412933254794	34.0	31.0	34.0	31.0	34.0
3	32.50551173119691	34.0	31.0	34.0	31.0	34.0
4	36.03658403561387	37.0	35.0	37.0	35.0	37.0
5	35.921759082806865	37.0	35.0	37.0	35.0	37.0
6	35.87858535226956	37.0	35.0	37.0	35.0	37.0
7	36.075275233655056	37.0	35.0	37.0	35.0	37.0
8	36.02613677049767	37.0	35.0	37.0	35.0	37.0
9	37.53575952169209	39.0	38.0	39.0	35.0	39.0
10	37.392825381425915	39.0	37.0	39.0	34.0	39.0
11	37.3072786934053	39.0	37.0	39.0	34.0	39.0
12	37.195716505806246	39.0	37.0	39.0	34.0	39.0
13	37.146048043816656	39.0	37.0	39.0	33.0	39.0
14	38.31758415495985	40.0	38.0	41.0	34.0	41.0
15	38.4057962366233	40.0	38.0	41.0	34.0	41.0
16	38.42777771361327	40.0	38.0	41.0	34.0	41.0
17	38.45489042627049	40.0	38.0	41.0	34.0	41.0
18	38.40316998333006	40.0	38.0	41.0	34.0	41.0
19	38.359765902210725	40.0	37.0	41.0	34.0	41.0
20	38.31240928742748	40.0	37.0	41.0	34.0	41.0
21	38.2860543576042	40.0	37.0	41.0	34.0	41.0
22	38.194144475371736	40.0	37.0	41.0	34.0	41.0
23	37.9545471461552	40.0	36.0	41.0	33.0	41.0
24	37.752764527806974	40.0	36.0	41.0	33.0	41.0
25	37.74393484992564	40.0	36.0	41.0	33.0	41.0
26	37.80092384057943	40.0	36.0	41.0	33.0	41.0
27	37.84514922739516	40.0	36.0	41.0	33.0	41.0
28	37.81658383028744	40.0	36.0	41.0	33.0	41.0
29	37.81526974117321	40.0	36.0	41.0	33.0	41.0
30	37.743473507116484	40.0	36.0	41.0	33.0	41.0
31	37.587555037106334	40.0	36.0	41.0	33.0	41.0
32	37.47831367974468	40.0	36.0	41.0	33.0	41.0
33	37.408274269904155	40.0	36.0	41.0	33.0	41.0
34	37.32985818360545	40.0	35.0	41.0	32.0	41.0
35	37.23584434717105	40.0	35.0	41.0	32.0	41.0
36	37.13009353901908	39.0	35.0	41.0	32.0	41.0
37	37.080510415824506	39.0	35.0	41.0	32.0	41.0
38	36.9823438524627	39.0	35.0	41.0	32.0	41.0
39	36.930444390546256	39.0	35.0	41.0	32.0	41.0
40	36.86753879065303	39.0	35.0	41.0	31.0	41.0
41	36.73237240566855	39.0	35.0	41.0	31.0	41.0
42	36.658242508472924	39.0	35.0	41.0	31.0	41.0
43	36.5837122247503	39.0	35.0	41.0	31.0	41.0
44	36.50040872791297	39.0	35.0	41.0	31.0	41.0
45	36.43136194300395	39.0	35.0	41.0	31.0	41.0
46	36.27895519649097	38.0	35.0	40.0	31.0	41.0
47	36.137796488148176	38.0	35.0	40.0	30.0	41.0
48	36.03259685311589	38.0	35.0	40.0	30.0	41.0
49	35.99052290223767	38.0	35.0	40.0	30.0	41.0
50	35.84532118827535	38.0	35.0	40.0	30.0	41.0
51	34.81993899238624	36.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	7.0
10	17.0
11	21.0
12	22.0
13	30.0
14	34.0
15	56.0
16	83.0
17	189.0
18	352.0
19	648.0
20	1077.0
21	1742.0
22	2633.0
23	3769.0
24	5329.0
25	7639.0
26	10188.0
27	12842.0
28	14760.0
29	17666.0
30	22184.0
31	27913.0
32	37053.0
33	52407.0
34	94958.0
35	121501.0
36	124800.0
37	197000.0
38	333353.0
39	467907.0
40	307.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.590860150889256	21.447756616323193	24.13939354274062	11.82198969004693
2	32.62174894481467	24.68415021167871	25.567567151365356	17.126533692141262
3	33.37292283447995	23.92578989716996	25.88800470197511	16.813282566374973
4	29.79491740102945	25.26605813047724	25.293135552655322	19.64588891583798
5	25.149471220293435	30.361297509005485	24.11783426821021	20.371397002490866
6	27.10289548756684	34.645882499387234	23.443208636029397	14.808013377016529
7	79.7397359245528	6.432106892936386	8.727784643380083	5.100372539130725
8	79.50187809513544	5.913401014055877	9.116814052540466	5.467906838268226
9	72.05982185366129	8.148764127420446	12.060681658062206	7.730732360856058
10	40.57436217271289	23.72052763757833	18.99538913848882	16.709721051219958
11	30.252282010710342	24.538881766628553	24.275935614766563	20.932900607894545
12	26.4758157554665	22.27342549923195	30.30335695870501	20.947401786596547
13	24.06862009093394	23.4732376255539	31.60634561313678	20.851796670375375
14	20.7808307250461	26.44437514677631	30.62963347950008	22.145160648677507
15	20.57242440458545	25.105261874604583	33.2583250240296	21.063988696780353
16	22.570314675577833	24.42056241474141	30.879682565348343	22.129440344332412
17	22.748499512991387	24.844882303043835	27.58432178757185	24.82229639639293
18	22.891778858308083	25.207475935101453	29.50502215600445	22.395723050586014
19	24.61664914975611	25.94896098412955	27.16231182154054	22.2720780445738
20	25.07189633068847	26.07119437097608	27.104435435747586	21.752473862587856
21	24.30288470792958	26.20350158550498	28.446051123713023	21.047562582852418
22	22.626330290652387	24.7327869083872	27.206906154274574	25.433976646685842
23	23.061429816220016	25.024222101592947	27.93927984323328	23.97506823895376
24	23.884532118827533	24.29640409266895	28.431999096563732	23.38706469193978
25	23.170316985500104	25.682036632800642	26.235391345747882	24.91225503595137
26	22.558251748162007	26.86105945868255	26.77341074139522	23.807278051760225
27	23.1740385269369	26.531510548003396	27.6968663337812	22.5975845912785
28	21.371208358838725	26.86831004803355	27.451822079520355	24.308659513607367
29	23.75639559728815	25.53920643903666	26.093459455089334	24.610938508585853
30	24.606703651088807	26.57206251676298	25.786303957538497	23.03492987460972
31	24.99457809911363	28.171619525002985	23.47394343513674	23.359858940746644
32	24.715077504308645	28.185414894122147	23.736247941923423	23.363259659645784
33	24.655789499350014	28.566167081811027	23.641541128807685	23.136502290031274
34	24.071058342220116	26.70032736731742	25.279661006073813	23.948953284388647
35	24.30051062115093	26.970139121485225	24.438464312342557	24.290885945021284
36	24.67324224539844	29.979454524688578	23.874265797622577	21.473037432290404
37	23.36550541740937	30.18401097469737	24.88132774332144	21.569155864571822
38	22.293316496566558	29.73684852171391	24.069646723054436	23.900188258665096
39	25.09955123343433	26.247903424716423	25.49762783815658	23.15491750369267
40	25.41337983976839	24.727910405814843	27.28332608274398	22.575383671672782
41	22.017216620660715	28.00992496602489	25.349985306327778	24.622873106986617
42	22.029343712584073	28.424748507212733	26.06266049147446	23.483247288728734
43	23.0992868756633	26.264072880614236	26.821213299505803	23.81542694421666
44	23.37974993808125	25.38200339558574	25.569363757576223	25.668882908756785
45	24.125341515591334	24.245264980166752	25.827818393911045	25.80157511033087
46	24.155434669623368	26.1163661842779	26.211201326408702	23.516997819690033
47	22.39995790808306	26.582328837967932	27.692823969806753	23.324889284142255
48	21.57608563138517	25.870744449449273	28.032831695213456	24.5203382239521
49	22.987127316499134	25.15107533298171	28.011529078713167	23.850268271805987
50	22.08048282508627	24.196949105995916	28.35249927173284	25.370068797184974
51	22.36620737712176	24.640518346557638	26.11142551719801	26.88184875912259
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	686.0
1	1698.0
2	2710.0
3	5059.5
4	7409.0
5	5076.5
6	2744.0
7	2624.5
8	2505.0
9	2611.0
10	2717.0
11	2873.5
12	3030.0
13	2958.5
14	2887.0
15	3051.5
16	3216.0
17	3394.5
18	3573.0
19	3483.5
20	3394.0
21	3990.5
22	4587.0
23	5535.0
24	6483.0
25	7875.0
26	11698.5
27	14130.0
28	16589.0
29	19048.0
30	21354.5
31	23661.0
32	25558.0
33	27455.0
34	30452.5
35	33450.0
36	36951.5
37	40453.0
38	43752.5
39	47052.0
40	53182.5
41	59313.0
42	66329.5
43	73346.0
44	83785.0
45	94224.0
46	109666.5
47	125109.0
48	141477.5
49	157846.0
50	156573.5
51	155301.0
52	135173.0
53	115045.0
54	104402.5
55	93760.0
56	87428.5
57	81097.0
58	78811.5
59	76526.0
60	71896.0
61	67266.0
62	62815.0
63	58364.0
64	52279.0
65	46194.0
66	38731.0
67	31268.0
68	27734.0
69	24200.0
70	19317.5
71	14435.0
72	12276.0
73	10117.0
74	8425.0
75	5399.0
76	4065.0
77	2988.5
78	1912.0
79	1478.0
80	1044.0
81	768.5
82	493.0
83	346.5
84	200.0
85	138.5
86	77.0
87	68.5
88	60.0
89	43.0
90	26.0
91	18.0
92	10.0
93	7.0
94	4.0
95	2.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1558494.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.722969005748787
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.64618546229899	19.585220099456954
2	11.509672278503292	6.3816457568654545
3	4.2835453853405046	3.562577878575391
4	2.068419424350656	2.2937091036864787
5	1.2551972742512982	1.7398897565084548
6	0.8951948438690028	1.4890475346411893
7	0.6458270023166208	1.253296937780953
8	0.5591539211961805	1.2401125461411688
9	0.45688520712317876	1.1399592992634902
>10	7.253704020998092	48.619099228345405
>50	0.3496874825930051	5.887919898535509
>100	0.06760729151990699	3.608499553874742
>500	0.004929697853340884	0.9318659336838779
>1k	0.0039907077860378585	2.267156472640915
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC	4492	0.2882269678291992	No Hit
GCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC	4488	0.2879703097990753	No Hit
CTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCT	4231	0.2714800313636113	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4180	0.26820764147953086	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCC	2332	0.1496316315622646	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1626	0.10433148924538689	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2832901506197648E-4	0.0	0.0	0.30202233694836167	0.0
2	1.2832901506197648E-4	0.0	0.0	1.1278837133797115	0.0
3	1.2832901506197648E-4	0.0	0.0	1.6365157645778552	0.0
4	1.2832901506197648E-4	0.0	0.0	2.227855865983443	0.0
5	1.9249352259296474E-4	0.0	0.0	3.6992763526840653	0.0
6	1.9249352259296474E-4	0.0	0.0	4.668673732462236	0.0
7	1.9249352259296474E-4	0.0	0.0	5.593220121476246	0.0
8	1.9249352259296474E-4	0.0	0.0	7.013180673137016	0.0
9	1.9249352259296474E-4	0.0	0.0	7.558450658135354	0.0
10	2.5665803012395297E-4	0.0	0.0	8.663427642326502	0.0
11	2.5665803012395297E-4	0.0	0.0	10.302381658190535	0.0
12	2.5665803012395297E-4	0.0	0.0	11.495520675729262	0.0
13	2.5665803012395297E-4	0.0	0.0	12.006013497645805	0.0
14	2.5665803012395297E-4	0.0	0.0	12.224301152266227	0.0
15	2.5665803012395297E-4	0.0	0.0	12.629371688309355	0.0
16	2.5665803012395297E-4	0.0	0.0	13.435855383466347	0.0
17	2.5665803012395297E-4	0.0	0.0	14.377662025006192	0.0
18	3.849870451859295E-4	0.0	0.0	15.384146490137274	0.0
19	3.849870451859295E-4	0.0	0.0	16.002820671751063	0.0
20	4.4915155271691776E-4	0.0	0.0	16.64927808512577	0.0
21	4.4915155271691776E-4	0.0	0.0	17.41886718845244	0.0
22	4.4915155271691776E-4	0.0	0.0	18.203534951048898	0.0
23	4.4915155271691776E-4	0.0	0.0	18.972867396345446	0.0
24	5.133160602479059E-4	0.0	0.0	19.609635969082973	0.0
25	5.133160602479059E-4	0.0	0.0	20.183394995425072	0.0
26	5.774805677788942E-4	0.0	0.0	20.69087208548766	0.0
27	5.774805677788942E-4	0.0	0.0	21.216700224704105	0.0
28	5.774805677788942E-4	0.0	0.0	21.740154277141908	0.0
29	5.774805677788942E-4	0.0	0.0	22.2903007647126	0.0
30	5.774805677788942E-4	0.0	0.0	22.898323638076246	0.0
31	5.774805677788942E-4	0.0	0.0	23.464896239574873	0.0
32	6.416450753098825E-4	0.0	0.0	24.03211048614881	0.0
33	6.416450753098825E-4	0.0	0.0	24.56717831444972	0.0
34	6.416450753098825E-4	0.0	0.0	25.091723163515546	0.0
35	7.69974090371859E-4	0.0	0.0	25.649376898467366	0.0
36	7.69974090371859E-4	0.0	0.0	26.19958755054559	0.0
37	7.69974090371859E-4	0.0	0.0	26.738697742820953	0.0
38	7.69974090371859E-4	0.0	0.0	27.275626341840265	0.0
39	7.69974090371859E-4	0.0	0.0	27.83571832807826	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGATAG	40	6.8175723E-9	45.0	1
GTATACG	40	6.8175723E-9	45.0	1
TATAGCG	40	6.8175723E-9	45.0	1
CGGTCTA	55	1.8189894E-12	45.0	31
TGCGACG	55	6.184564E-11	40.909092	1
TACGAAT	255	0.0	40.588234	12
AATTGCG	50	1.0822987E-9	40.500004	1
GTACGAG	195	0.0	39.23077	1
TAACGCC	35	6.249724E-6	38.571426	12
GCTACGA	275	0.0	37.636364	10
TCGAATA	30	1.1401085E-4	37.499996	22
CGACAGG	170	0.0	37.058823	2
CGAATAT	280	0.0	36.964283	14
TAGCACG	55	2.750312E-9	36.81818	1
CTACGAA	295	0.0	36.61017	11
CGTTCGG	285	0.0	36.31579	45
TACGAGG	355	0.0	36.126762	2
TAGGGTC	405	0.0	36.11111	5
GATCGAG	50	4.8808943E-8	36.000004	1
ACGTTAC	25	0.002107338	36.000004	28
>>END_MODULE