FastQCFastQC Report
Sat 18 Jun 2016
SRR3549972_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549972_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1637522
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT176541.078092385934357TruSeq Adapter, Index 27 (100% over 25bp)
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC106170.648357701453782TruSeq Adapter, Index 27 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC95180.5812440993159176TruSeq Adapter, Index 27 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC45540.2781031338815601TruSeq Adapter, Index 27 (96% over 25bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCC45240.2762710974264773No Hit
ACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC34360.20982924198880995TruSeq Adapter, Index 27 (100% over 24bp)
CGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG27760.16952443997698963TruSeq Adapter, Index 27 (100% over 23bp)
CGTTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC25770.15737193149160744TruSeq Adapter, Index 27 (100% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25660.15670018479141043No Hit
GCCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG23220.14179962162340415TruSeq Adapter, Index 27 (95% over 24bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTC22990.14039506034117405No Hit
AGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG21350.13037992772005505TruSeq Adapter, Index 27 (100% over 23bp)
GGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG21310.1301356561927107TruSeq Adapter, Index 27 (95% over 24bp)
CGTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT18790.11474654997001568TruSeq Adapter, Index 27 (100% over 22bp)
ACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG18550.11328092080594948TruSeq Adapter, Index 27 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCGA253.8915336E-545.00000412
CCGTAAG302.166069E-645.0000041
TGCTCGA207.034166E-445.021
CTCGACG207.034166E-445.01
ACGTAAG453.8562575E-1045.01
TATCGTG207.034166E-445.025
ACGACCG207.034166E-445.01
TAGTACG650.041.5384641
CGTTTTT25500.040.5882381
CGAACGG1000.040.5000042
TAGGACG501.0822987E-940.5000041
CGATTCG451.9292202E-840.010
TGTAGCG1250.039.6000021
GCCGCAT356.249915E-638.5714319
CGTTAGG2100.038.571432
TTGCGTA356.249915E-638.571431
ACGGGAT7000.038.5714265
TCTACGG1000.038.2500042
ACAACGA5650.038.2300913
CGCACGG1600.037.968752
Produced by FastQC (version 0.11.5)