##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549972_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1637522
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.07902428181117	33.0	31.0	34.0	30.0	34.0
2	32.24171950056243	34.0	31.0	34.0	30.0	34.0
3	32.25168761091454	34.0	31.0	34.0	30.0	34.0
4	35.83592525779806	37.0	35.0	37.0	35.0	37.0
5	35.71334919469784	37.0	35.0	37.0	33.0	37.0
6	35.67479215546417	37.0	35.0	37.0	33.0	37.0
7	35.96567130090466	37.0	35.0	37.0	35.0	37.0
8	35.9505728777995	37.0	35.0	37.0	35.0	37.0
9	37.483188622809344	39.0	37.0	39.0	35.0	39.0
10	37.20341894643248	39.0	37.0	39.0	34.0	39.0
11	37.13983506786474	39.0	37.0	39.0	34.0	39.0
12	37.08350788569558	39.0	37.0	39.0	33.0	39.0
13	37.06453348413029	39.0	37.0	39.0	33.0	39.0
14	38.101670084432456	40.0	37.0	41.0	33.0	41.0
15	38.19772680916653	40.0	37.0	41.0	33.0	41.0
16	38.2006824946474	40.0	37.0	41.0	33.0	41.0
17	38.225829637708685	40.0	37.0	41.0	34.0	41.0
18	38.21425666342193	40.0	37.0	41.0	34.0	41.0
19	38.18746984773335	40.0	37.0	41.0	34.0	41.0
20	38.1535533568404	40.0	37.0	41.0	34.0	41.0
21	38.12106463302478	40.0	37.0	41.0	34.0	41.0
22	38.03174613837249	40.0	37.0	41.0	34.0	41.0
23	37.753981320556306	39.0	36.0	41.0	33.0	41.0
24	37.5427053804468	39.0	36.0	41.0	32.0	41.0
25	37.589448569240595	39.0	36.0	41.0	33.0	41.0
26	37.70810590636339	39.0	36.0	41.0	33.0	41.0
27	37.81192374820002	40.0	37.0	41.0	33.0	41.0
28	37.81317808249294	40.0	37.0	41.0	33.0	41.0
29	37.83402910006705	40.0	37.0	41.0	33.0	41.0
30	37.76567887332201	40.0	36.0	41.0	33.0	41.0
31	37.68005437484199	40.0	36.0	41.0	33.0	41.0
32	37.592511734193494	40.0	36.0	41.0	33.0	41.0
33	37.57480082710339	40.0	36.0	41.0	33.0	41.0
34	37.58019861717889	40.0	36.0	41.0	33.0	41.0
35	37.512576930264146	40.0	36.0	41.0	33.0	41.0
36	37.42774631424799	40.0	36.0	41.0	33.0	41.0
37	37.38121319896771	40.0	36.0	41.0	33.0	41.0
38	37.29483512282583	39.0	36.0	41.0	32.0	41.0
39	37.25399719820558	39.0	36.0	41.0	32.0	41.0
40	37.21175837637601	39.0	36.0	41.0	32.0	41.0
41	37.15209871989506	39.0	35.0	41.0	32.0	41.0
42	37.11227696482857	39.0	35.0	41.0	32.0	41.0
43	36.986055149182725	39.0	35.0	41.0	31.0	41.0
44	36.89721725876049	39.0	35.0	41.0	31.0	41.0
45	36.825428299589255	39.0	35.0	41.0	31.0	41.0
46	36.68063390904061	39.0	35.0	41.0	31.0	41.0
47	36.578499098027386	39.0	35.0	40.0	31.0	41.0
48	36.55965599240804	39.0	35.0	40.0	31.0	41.0
49	36.54975749944123	39.0	35.0	40.0	31.0	41.0
50	36.460184351721686	39.0	35.0	40.0	31.0	41.0
51	35.42810966814492	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	13.0
11	16.0
12	15.0
13	17.0
14	23.0
15	49.0
16	95.0
17	138.0
18	263.0
19	509.0
20	904.0
21	1521.0
22	2370.0
23	3681.0
24	5322.0
25	7160.0
26	9352.0
27	11565.0
28	14573.0
29	18141.0
30	23976.0
31	30701.0
32	40703.0
33	56535.0
34	94685.0
35	119970.0
36	129366.0
37	203919.0
38	374771.0
39	486909.0
40	255.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.24419580317089	21.01889318128245	24.512159225952384	12.224751789594277
2	31.957188972117628	23.404265713682015	25.789516110317905	18.849029203882452
3	31.634628420259393	23.610491950642494	27.374594051255496	17.38028557784262
4	27.70271178036081	25.345980084542376	27.251114794182918	19.700193340913895
5	23.983128165606324	28.904710898540596	26.163373682918454	20.94878725293462
6	26.06713070114478	32.283474664767866	25.906827511325037	15.742567122762322
7	77.28635096200234	5.5390400861789955	10.443157404908147	6.731451546910515
8	75.41010136047028	4.516946947888334	11.72692641686646	8.346025274774934
9	68.61898649300589	7.237826423095384	14.974272101382455	9.168914982516267
10	34.022321532168725	29.32357550005435	20.77840786261192	15.875695105165
11	24.951847975172242	24.267826630726184	29.86451479735845	20.91581059674313
12	23.016301460377324	22.726351157419565	33.167126914936105	21.090220467267002
13	22.605375683502267	24.086699293200336	34.04259606893831	19.265328954359088
14	18.343875685334304	28.287497816823226	31.084467872798044	22.284158625044427
15	17.00636693736023	26.096687555953444	35.28471678548441	21.612228721201916
16	18.684817669625204	27.393586162506516	31.550843286380275	22.370752881488006
17	18.179664151077056	27.153467251127005	29.695356764672475	24.971511833123465
18	20.02366991099967	25.606373532691467	32.4635638483025	21.90639270800637
19	19.27027545278781	27.842801501292808	29.734806616338588	23.152116429580794
20	21.496382949358846	28.075347995324645	29.422261197101474	21.006007858215035
21	19.958021938025873	29.253835978997532	30.427194260596192	20.3609478223804
22	18.749183217080443	25.831714016666645	28.457632935618577	26.96146983063434
23	18.89531865831421	27.39749450694403	29.052128765292924	24.655058069448838
24	20.18018689214557	24.942321385605812	30.05797784701518	24.819513875233433
25	18.323173673391867	27.723108452894067	26.594879335972283	27.358838537741782
26	18.088917278668625	27.426868158107187	28.490914931219248	25.993299632004945
27	20.72888181044285	26.21460963577894	27.791809819959674	25.264698733818534
28	15.921434948660233	26.64458859178686	27.75779500977697	29.67618144977594
29	17.528802666467993	24.591425336575632	27.502531263702107	30.37724073325427
30	20.22171305179411	25.989024880276418	28.381542354850804	25.407719713078663
31	18.244335037941475	26.807884107816566	26.972462049364832	27.975318804877126
32	17.628282245978983	25.53871031961708	26.66797759053008	30.165029843873853
33	17.425597946165	25.494863580458766	29.045838773463807	28.03369969991243
34	15.369991975680328	22.52000278469541	31.628643767839453	30.48136147178481
35	15.973281580339075	23.435959944354945	29.991535991577518	30.59922248372846
36	16.722095947413226	25.83385139253091	29.828118339784137	27.61593432027173
37	16.012303956832337	25.451932859527993	31.464493301464042	27.07126988217563
38	16.02457860108139	24.773041217156166	30.706213412705296	28.496166769057147
39	19.33476313600672	21.280507987068265	31.889586826925076	27.495142049999938
40	18.794251313875478	21.051320226537413	35.737229789889845	24.417198669697264
41	16.388726380470004	23.281946746364323	30.749327337281574	29.5799995358841
42	19.515707269887063	22.248067506879295	29.65132682186865	28.58489840136499
43	20.190568432057706	21.657235750115113	29.801004200248915	28.35119161757827
44	18.794373449639153	22.42143922341196	27.732757178224173	31.05143014872472
45	19.329083822995965	20.373588873920472	30.167045083974443	30.130282219109116
46	20.75330896317729	21.572717801653962	31.745039150619046	25.928934084549702
47	16.44332106683147	22.1226951454698	33.593014322861	27.840969464837723
48	16.62530335470302	20.911841184423782	32.67943881059308	29.78341665028012
49	20.403023592965468	18.866616753851247	33.43918432851589	27.291175324667392
50	17.792127372945217	19.049453992068504	33.292071801172746	29.86634683381353
51	17.07885451309967	18.551384347813343	30.833417810569873	33.53634332851711
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	797.0
1	1319.5
2	1842.0
3	3290.5
4	4739.0
5	3393.0
6	2047.0
7	2067.0
8	2087.0
9	2225.0
10	2363.0
11	2552.0
12	2741.0
13	2819.0
14	2897.0
15	2939.5
16	2982.0
17	3023.5
18	3065.0
19	3429.5
20	3794.0
21	3893.5
22	3993.0
23	4443.5
24	4894.0
25	6238.5
26	9691.5
27	11800.0
28	13864.0
29	15928.0
30	18531.5
31	21135.0
32	24604.0
33	28073.0
34	32974.5
35	37876.0
36	42403.0
37	46930.0
38	50363.5
39	53797.0
40	60534.5
41	67272.0
42	79481.5
43	91691.0
44	105042.5
45	118394.0
46	146272.0
47	174150.0
48	210953.5
49	247757.0
50	234456.5
51	221156.0
52	179730.5
53	138305.0
54	113849.5
55	89394.0
56	75571.5
57	61749.0
58	54321.0
59	46893.0
60	41835.0
61	36777.0
62	33192.0
63	29607.0
64	24538.0
65	19469.0
66	16018.5
67	12568.0
68	10268.5
69	7969.0
70	6498.0
71	5027.0
72	4145.5
73	3264.0
74	2521.5
75	1610.0
76	1441.0
77	1142.0
78	843.0
79	586.5
80	330.0
81	269.5
82	209.0
83	135.5
84	62.0
85	43.5
86	25.0
87	17.0
88	9.0
89	7.5
90	6.0
91	3.5
92	1.0
93	5.5
94	10.0
95	6.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1637522.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.429525351996308
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.92504581084542	20.669269035366312
2	13.495717352851585	8.213365466639477
3	5.409424194209825	4.938186319722302
4	2.682862372714374	3.2655291434571603
5	1.6094199664269226	2.4486942835198544
6	1.0559624750618775	1.92794621433913
7	0.7592376645335323	1.617226923277951
8	0.5813247960441841	1.4151550095176466
9	0.46851385189347045	1.2830988720558423
>10	5.71850466153723	38.960504325138764
>50	0.20515397799129562	4.016950484224989
>100	0.07541648210230145	4.3317686133227795
>500	0.00833442644407632	1.7023593870125375
>1k	0.0044721312626750985	2.8724415121071623
>5k	2.03278693757959E-4	0.5887524670464493
>10k+	4.06557387515918E-4	1.7487519432517515
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT	17654	1.078092385934357	TruSeq Adapter, Index 27 (100% over 25bp)
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	10617	0.648357701453782	TruSeq Adapter, Index 27 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	9518	0.5812440993159176	TruSeq Adapter, Index 27 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	4554	0.2781031338815601	TruSeq Adapter, Index 27 (96% over 25bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCC	4524	0.2762710974264773	No Hit
ACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	3436	0.20982924198880995	TruSeq Adapter, Index 27 (100% over 24bp)
CGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG	2776	0.16952443997698963	TruSeq Adapter, Index 27 (100% over 23bp)
CGTTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC	2577	0.15737193149160744	TruSeq Adapter, Index 27 (100% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2566	0.15670018479141043	No Hit
GCCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG	2322	0.14179962162340415	TruSeq Adapter, Index 27 (95% over 24bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTC	2299	0.14039506034117405	No Hit
AGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG	2135	0.13037992772005505	TruSeq Adapter, Index 27 (100% over 23bp)
GGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG	2131	0.1301356561927107	TruSeq Adapter, Index 27 (95% over 24bp)
CGTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT	1879	0.11474654997001568	TruSeq Adapter, Index 27 (100% over 22bp)
ACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG	1855	0.11328092080594948	TruSeq Adapter, Index 27 (100% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8320364550827409E-4	0.0	0.0	1.1647477102597705	0.0
2	1.8320364550827409E-4	0.0	0.0	3.0264020880330156	0.0
3	1.8320364550827409E-4	0.0	0.0	4.209958705898303	0.0
4	1.8320364550827409E-4	0.0	0.0	5.665755940988884	0.0
5	1.8320364550827409E-4	0.0	0.0	8.746874851147037	0.0
6	1.8320364550827409E-4	0.0	0.0	10.400409887622883	0.0
7	1.8320364550827409E-4	0.0	0.0	12.03690698506646	0.0
8	1.8320364550827409E-4	0.0	0.0	15.074484495475481	0.0
9	1.8320364550827409E-4	0.0	0.0	16.274407305672838	0.0
10	1.8320364550827409E-4	0.0	0.0	18.435904983261295	0.0
11	1.8320364550827409E-4	0.0	0.0	21.516107875191906	0.0
12	1.8320364550827409E-4	0.0	0.0	23.698796107777483	0.0
13	1.8320364550827409E-4	0.0	0.0	24.591852811748485	0.0
14	1.8320364550827409E-4	0.0	0.0	24.961740972029688	0.0
15	1.8320364550827409E-4	0.0	0.0	25.652846190768734	0.0
16	1.8320364550827409E-4	0.0	0.0	26.910478149301202	0.0
17	1.8320364550827409E-4	0.0	0.0	28.482609699289537	0.0
18	1.8320364550827409E-4	0.0	0.0	29.979200279446626	0.0
19	1.8320364550827409E-4	0.0	0.0	30.955736777887566	0.0
20	2.4427152734436545E-4	0.0	0.0	31.93850220027578	0.0
21	2.4427152734436545E-4	0.0	0.0	33.12095959626802	0.0
22	2.4427152734436545E-4	0.0	0.0	34.37010311922527	0.0
23	2.4427152734436545E-4	0.0	0.0	35.5767433964246	0.0
24	2.4427152734436545E-4	0.0	0.0	36.53899001051589	0.0
25	2.4427152734436545E-4	0.0	0.0	37.36059729273866	0.0
26	2.4427152734436545E-4	0.0	0.0	38.086938679297134	0.0
27	2.4427152734436545E-4	0.0	0.0	38.83062334429705	0.0
28	2.4427152734436545E-4	0.0	0.0	39.55885783519244	0.0
29	2.4427152734436545E-4	0.0	0.0	40.30645084462987	0.0
30	2.4427152734436545E-4	0.0	0.0	41.13929461711049	0.0
31	4.2747517285263956E-4	0.0	0.0	41.851101847791966	0.0
32	4.885430546887309E-4	0.0	0.0	42.55222219915213	0.0
33	5.496109365248223E-4	0.0	0.0	43.25303721110312	0.0
34	6.106788183609137E-4	0.0	0.0	43.93815777742223	0.0
35	6.106788183609137E-4	0.0	0.0	44.65546111746895	0.0
36	6.106788183609137E-4	0.0	0.0	45.315177444944254	0.0
37	6.106788183609137E-4	0.0	0.0	45.946924682538615	0.0
38	6.106788183609137E-4	0.0	0.0	46.578916191660326	0.0
39	6.106788183609137E-4	0.0	0.0	47.26983820675386	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCCGA	25	3.8915336E-5	45.000004	12
CCGTAAG	30	2.166069E-6	45.000004	1
TGCTCGA	20	7.034166E-4	45.0	21
CTCGACG	20	7.034166E-4	45.0	1
ACGTAAG	45	3.8562575E-10	45.0	1
TATCGTG	20	7.034166E-4	45.0	25
ACGACCG	20	7.034166E-4	45.0	1
TAGTACG	65	0.0	41.538464	1
CGTTTTT	2550	0.0	40.588238	1
CGAACGG	100	0.0	40.500004	2
TAGGACG	50	1.0822987E-9	40.500004	1
CGATTCG	45	1.9292202E-8	40.0	10
TGTAGCG	125	0.0	39.600002	1
GCCGCAT	35	6.249915E-6	38.57143	19
CGTTAGG	210	0.0	38.57143	2
TTGCGTA	35	6.249915E-6	38.57143	1
ACGGGAT	700	0.0	38.571426	5
TCTACGG	100	0.0	38.250004	2
ACAACGA	565	0.0	38.23009	13
CGCACGG	160	0.0	37.96875	2
>>END_MODULE