FastQCFastQC Report
Sat 18 Jun 2016
SRR3549971_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549971_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1090040
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC56810.5211735349161498No Hit
CTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCT56060.5142930534659278No Hit
CCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC46640.4278742064511394No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCC20380.18696561594069944No Hit
TCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC20100.18439690286594987No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16990.15586583978569593No Hit
ACTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC16790.15403104473230342No Hit
CGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG15380.141095739605886No Hit
GCCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG12660.11614252687974753No Hit
AGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG10980.10073024843125022No Hit
GGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG10910.10008807016256285No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAATA351.2118471E-745.00000434
CTTACGA351.2118471E-745.00000429
CTAGACG253.8906735E-545.01
TAATACG207.0331275E-445.01
CGTTAGG950.045.02
ACGATAG207.0331275E-445.01
TCGACCT253.8906735E-545.023
TCGAACA207.0331275E-445.032
TACCGGT302.1653996E-644.99999640
CGTAAGG850.039.7058832
TACGGGA2500.039.64
TCACGAC800.039.37525
GTTCACG403.458008E-739.3751
TATACGG800.039.3752
GTAGGGT3150.039.2857134
CTAGTCG356.2479903E-638.571431
TTAACGG700.038.571432
CCGGTAT356.2479903E-638.5714342
TATGCGG950.037.8947372
ACGGGTA1250.037.85