##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549971_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1090040
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.28203827382482	33.0	31.0	34.0	30.0	34.0
2	32.443004843858944	34.0	31.0	34.0	31.0	34.0
3	32.44518091079227	34.0	31.0	34.0	30.0	34.0
4	35.990002201754066	37.0	35.0	37.0	35.0	37.0
5	35.879220028622804	37.0	35.0	37.0	35.0	37.0
6	35.8263265568236	37.0	35.0	37.0	35.0	37.0
7	36.04751568749771	37.0	35.0	37.0	35.0	37.0
8	36.00640343473634	37.0	35.0	37.0	35.0	37.0
9	37.54636985798686	39.0	38.0	39.0	35.0	39.0
10	37.375726578841146	39.0	37.0	39.0	34.0	39.0
11	37.26922406517192	39.0	37.0	39.0	34.0	39.0
12	37.11890664562768	39.0	37.0	39.0	34.0	39.0
13	37.041742504862206	39.0	37.0	39.0	33.0	39.0
14	38.15726303621886	40.0	37.0	41.0	33.0	41.0
15	38.25931433708855	40.0	37.0	41.0	34.0	41.0
16	38.291518659865694	40.0	37.0	41.0	34.0	41.0
17	38.30240358151995	40.0	37.0	41.0	34.0	41.0
18	38.26274356904334	40.0	37.0	41.0	34.0	41.0
19	38.21059594143334	40.0	37.0	41.0	34.0	41.0
20	38.171453341161794	40.0	37.0	41.0	34.0	41.0
21	38.147010201460496	40.0	37.0	41.0	34.0	41.0
22	38.04880279622766	40.0	36.0	41.0	34.0	41.0
23	37.79516714982936	40.0	36.0	41.0	33.0	41.0
24	37.577964111408754	39.0	36.0	41.0	33.0	41.0
25	37.58154746614803	39.0	36.0	41.0	33.0	41.0
26	37.65966936993138	39.0	36.0	41.0	33.0	41.0
27	37.74844868078236	40.0	36.0	41.0	33.0	41.0
28	37.7427525595391	40.0	36.0	41.0	33.0	41.0
29	37.71704891563612	40.0	36.0	41.0	33.0	41.0
30	37.66386462882096	40.0	36.0	41.0	33.0	41.0
31	37.51760852812741	40.0	36.0	41.0	33.0	41.0
32	37.36513705919049	39.0	35.0	41.0	33.0	41.0
33	37.31837088547209	39.0	35.0	41.0	33.0	41.0
34	37.22906590583832	39.0	35.0	41.0	32.0	41.0
35	37.136591317749804	39.0	35.0	41.0	32.0	41.0
36	37.046544163516934	39.0	35.0	41.0	32.0	41.0
37	37.004325529338374	39.0	35.0	41.0	32.0	41.0
38	36.89309658361161	39.0	35.0	41.0	31.0	41.0
39	36.85582730908958	39.0	35.0	41.0	31.0	41.0
40	36.794030494293786	39.0	35.0	41.0	31.0	41.0
41	36.681860298704635	39.0	35.0	41.0	31.0	41.0
42	36.62524219294705	39.0	35.0	41.0	31.0	41.0
43	36.53749954130124	39.0	35.0	41.0	31.0	41.0
44	36.44008476753147	39.0	35.0	40.0	31.0	41.0
45	36.36860757403398	38.0	35.0	40.0	31.0	41.0
46	36.2323125756853	38.0	35.0	40.0	31.0	41.0
47	36.08529962203222	38.0	35.0	40.0	30.0	41.0
48	36.012432571281785	38.0	35.0	40.0	30.0	41.0
49	35.995613922424866	38.0	35.0	40.0	30.0	41.0
50	35.90731165828777	38.0	35.0	40.0	30.0	41.0
51	34.846309309750104	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	13.0
11	8.0
12	19.0
13	16.0
14	25.0
15	41.0
16	56.0
17	155.0
18	254.0
19	482.0
20	798.0
21	1282.0
22	2001.0
23	2842.0
24	4112.0
25	5380.0
26	6966.0
27	8542.0
28	10406.0
29	12495.0
30	15589.0
31	20162.0
32	26547.0
33	38056.0
34	70007.0
35	93064.0
36	88241.0
37	136775.0
38	236414.0
39	309093.0
40	194.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.021907452937505	21.58287769256174	23.764724230303475	11.630490624197277
2	32.926956808924444	23.63564639829731	25.29053979670471	18.146856996073538
3	31.34866610399618	24.261586730762176	26.402517338813254	17.98722982642839
4	29.896242339730648	25.303291622325787	25.810704194341493	18.98976184360207
5	25.332648343180065	28.775366041613154	24.750651352243956	21.141334262962825
6	26.484165718689223	33.69692855308062	24.405618142453488	15.413287585776668
7	77.8388866463616	6.5181094271769835	9.587446332244689	6.055557594216726
8	76.77296246009321	5.640343473633995	10.499431213533448	7.087262852739349
9	70.55383288686654	7.618527760449158	13.318960772081759	8.508678580602547
10	39.93091996623977	23.081996990936112	19.866518659865694	17.120564382958424
11	30.11761036292246	23.71949653223735	25.158618032365787	21.004275072474403
12	27.06735532641004	21.52223771604712	30.83116215918682	20.579244798356022
13	24.052328354922757	23.128417305786943	32.049099115628785	20.77015522366152
14	19.04425525668783	27.865399434883127	30.980147517522294	22.110197790906756
15	18.326208212542657	25.193754357638255	35.37218817658068	21.107849253238413
16	20.866940662727973	23.985908773989944	32.35771164360941	22.789438919672673
17	20.276320135040915	24.70468973615647	28.6302337528898	26.38875637591281
18	21.92002128362262	24.976422883563906	30.284668452533854	22.818887380279623
19	23.110161095005687	25.944919452497157	28.202634765696672	22.742284686800485
20	24.83239147187259	25.315309529925507	27.542108546475358	22.31019045172654
21	23.678488862794026	25.890609518916737	29.41699387178452	21.013907746504714
22	21.761678470514845	24.84706983229973	27.023962423397307	26.36728927378812
23	21.906810759238194	25.800796301053175	27.782283218964444	24.510109720744193
24	23.635554658544642	23.87444497449635	28.07282301566915	24.41717735128986
25	21.611133536383985	25.15448974349565	26.678837473854173	26.555539246266193
26	19.56258485927122	27.41651682507064	27.84540016880114	25.175498146856995
27	21.518109427176984	26.691497559722578	27.530641077391653	24.25975193570878
28	19.81541961762871	26.795897398260614	27.973193644269934	25.41548933984074
29	21.54150306410774	26.167938791237017	26.647554218193825	25.643003926461418
30	23.49537631646545	25.673369784595064	25.456772228542075	25.374481670397415
31	23.951598106491506	27.892095702909987	23.238321529485155	24.917984661113355
32	23.88114197644123	29.36286741770944	22.984752853106308	23.77123775274302
33	23.149700928406297	27.53761329859455	23.16364537081208	26.149040402187072
34	22.65293016770027	27.657608895086415	25.27815493009431	24.411306007119006
35	22.66503981505266	27.66770026788008	24.093702983376758	25.573556933690504
36	22.282393306667643	29.453414553594364	24.385802355876848	23.87838978386114
37	23.019705698873434	28.941323254192508	25.779696157939156	22.2592748889949
38	22.50275219258009	29.257641921397383	23.81527283402444	24.424333051998094
39	24.453231074089025	25.998678947561558	24.880371362518808	24.667718615830612
40	24.481853876921946	24.639187552750357	27.21019412131665	23.668764449011046
41	20.805383288686656	27.730450258706103	24.798906462148178	26.665259990459067
42	21.925525668782797	27.913471065282007	25.297053319144254	24.863949946790946
43	23.044842391104915	25.229624600932077	26.763605005320905	24.961928002642107
44	22.407801548567026	25.02201754064071	25.775751348574367	26.794429562217903
45	23.688305016329675	23.38519687350923	25.609151957726322	27.317346152434773
46	24.150122931268577	24.95064401306374	25.942442479175078	24.956790576492605
47	21.124729367729625	26.136839015082014	28.33767568162636	24.400755935562
48	20.889233422626692	25.19439653590694	28.156030971340503	25.760339070125866
49	22.70925837583942	23.6477560456497	28.890315951708196	24.752669626802685
50	21.368665370078162	22.876316465450806	28.821235917947963	26.933782246523062
51	20.610711533521705	23.132820813915085	26.43976367839712	29.816703974166085
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	799.0
1	1375.5
2	1952.0
3	2838.0
4	3724.0
5	2759.5
6	1795.0
7	1787.0
8	1779.0
9	1900.0
10	2021.0
11	2105.5
12	2190.0
13	2309.0
14	2428.0
15	2614.5
16	2801.0
17	2789.0
18	2777.0
19	2948.5
20	3120.0
21	3500.0
22	3880.0
23	4040.0
24	4200.0
25	4997.0
26	6484.5
27	7175.0
28	9107.5
29	11040.0
30	11871.5
31	12703.0
32	14797.0
33	16891.0
34	19256.5
35	21622.0
36	23680.0
37	25738.0
38	27980.5
39	30223.0
40	34556.5
41	38890.0
42	44967.0
43	51044.0
44	59908.0
45	68772.0
46	81303.5
47	93835.0
48	110011.0
49	126187.0
50	123702.0
51	121217.0
52	103911.0
53	86605.0
54	76150.5
55	65696.0
56	57982.5
57	50269.0
58	50017.5
59	49766.0
60	47203.0
61	44640.0
62	41804.0
63	38968.0
64	34916.0
65	30864.0
66	25673.0
67	20482.0
68	17429.0
69	14376.0
70	11765.0
71	9154.0
72	7507.0
73	5860.0
74	4850.5
75	3092.0
76	2343.0
77	1736.0
78	1129.0
79	949.5
80	770.0
81	582.0
82	394.0
83	261.0
84	128.0
85	102.5
86	77.0
87	67.0
88	57.0
89	32.0
90	7.0
91	9.0
92	11.0
93	6.5
94	2.0
95	2.0
96	2.0
97	1.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1090040.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.254159476016422
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.56082077229665	19.653825233830652
2	12.40897629519969	7.01210390357359
3	4.994460216152274	4.233428263313574
4	2.5098949955654977	2.836598938910524
5	1.4741025582919316	2.082476438299201
6	0.965276327085278	1.6363842770334498
7	0.6997076393112046	1.3838755859380025
8	0.5070971792635038	1.14620836581993
9	0.40785638616563186	1.037127544023195
>10	5.771489008815576	40.844158709637554
>50	0.6200944515529014	10.760365189539415
>100	0.07200448071995874	3.923283867092484
>500	0.004274238581541025	0.7166939368404761
>1k	0.0032878758319546345	1.684950696877843
>5k	6.57575166390927E-4	1.0485190492700525
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC	5681	0.5211735349161498	No Hit
CTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCT	5606	0.5142930534659278	No Hit
CCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC	4664	0.4278742064511394	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCC	2038	0.18696561594069944	No Hit
TCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC	2010	0.18439690286594987	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1699	0.15586583978569593	No Hit
ACTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC	1679	0.15403104473230342	No Hit
CGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG	1538	0.141095739605886	No Hit
GCCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG	1266	0.11614252687974753	No Hit
AGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG	1098	0.10073024843125022	No Hit
GGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG	1091	0.10008807016256285	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.834795053392536E-4	0.0	0.0	0.5481450222010201	0.0
2	1.834795053392536E-4	0.0	0.0	1.9296539576529301	0.0
3	1.834795053392536E-4	0.0	0.0	2.7983376756816263	0.0
4	1.834795053392536E-4	0.0	0.0	3.6896811126197204	0.0
5	1.834795053392536E-4	0.0	0.0	5.72263403177865	0.0
6	1.834795053392536E-4	0.0	0.0	6.913140802172397	0.0
7	1.834795053392536E-4	0.0	0.0	8.066676452240285	0.0
8	2.752192580088804E-4	0.0	0.0	9.997981725441269	0.0
9	2.752192580088804E-4	0.0	0.0	10.701809107922646	0.0
10	2.752192580088804E-4	0.0	0.0	12.106803420057979	0.0
11	2.752192580088804E-4	0.0	0.0	14.264522402847602	0.0
12	2.752192580088804E-4	0.0	0.0	15.857307988697663	0.0
13	2.752192580088804E-4	0.0	0.0	16.507192396609298	0.0
14	2.752192580088804E-4	0.0	0.0	16.788466478294374	0.0
15	2.752192580088804E-4	0.0	0.0	17.293035117977322	0.0
16	2.752192580088804E-4	0.0	0.0	18.31850207331841	0.0
17	2.752192580088804E-4	0.0	0.0	19.568639682947413	0.0
18	2.752192580088804E-4	0.0	0.0	20.928589776521964	0.0
19	2.752192580088804E-4	0.0	0.0	21.734340024219296	0.0
20	2.752192580088804E-4	0.0	0.0	22.53862243587391	0.0
21	2.752192580088804E-4	0.0	0.0	23.488679314520567	0.0
22	2.752192580088804E-4	0.0	0.0	24.426259586804154	0.0
23	2.752192580088804E-4	0.0	0.0	25.316226927452202	0.0
24	2.752192580088804E-4	0.0	0.0	26.036383985908774	0.0
25	2.752192580088804E-4	0.0	0.0	26.665718689222413	0.0
26	2.752192580088804E-4	0.0	0.0	27.27110931708928	0.0
27	2.752192580088804E-4	0.0	0.0	27.844849730285127	0.0
28	2.752192580088804E-4	0.0	0.0	28.43923158783164	0.0
29	2.752192580088804E-4	0.0	0.0	29.055630986018862	0.0
30	2.752192580088804E-4	0.0	0.0	29.679186084914313	0.0
31	3.669590106785072E-4	0.0	0.0	30.301548567025062	0.0
32	3.669590106785072E-4	0.0	0.0	30.908682250192655	0.0
33	3.669590106785072E-4	0.0	0.0	31.515907673112913	0.0
34	3.669590106785072E-4	0.0	0.0	32.10028989761844	0.0
35	3.669590106785072E-4	0.0	0.0	32.688433451983414	0.0
36	3.669590106785072E-4	0.0	0.0	33.26547649627537	0.0
37	3.669590106785072E-4	0.0	0.0	33.846189130674105	0.0
38	3.669590106785072E-4	0.0	0.0	34.45084584051961	0.0
39	3.669590106785072E-4	0.0	0.0	35.04715423287219	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAATA	35	1.2118471E-7	45.000004	34
CTTACGA	35	1.2118471E-7	45.000004	29
CTAGACG	25	3.8906735E-5	45.0	1
TAATACG	20	7.0331275E-4	45.0	1
CGTTAGG	95	0.0	45.0	2
ACGATAG	20	7.0331275E-4	45.0	1
TCGACCT	25	3.8906735E-5	45.0	23
TCGAACA	20	7.0331275E-4	45.0	32
TACCGGT	30	2.1653996E-6	44.999996	40
CGTAAGG	85	0.0	39.705883	2
TACGGGA	250	0.0	39.6	4
TCACGAC	80	0.0	39.375	25
GTTCACG	40	3.458008E-7	39.375	1
TATACGG	80	0.0	39.375	2
GTAGGGT	315	0.0	39.285713	4
CTAGTCG	35	6.2479903E-6	38.57143	1
TTAACGG	70	0.0	38.57143	2
CCGGTAT	35	6.2479903E-6	38.57143	42
TATGCGG	95	0.0	37.894737	2
ACGGGTA	125	0.0	37.8	5
>>END_MODULE