SRR3549968_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549968_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1671754
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	5189	0.31039255775670344	RNA PCR Primer, Index 27 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCT	4575	0.27366466597358224	RNA PCR Primer, Index 38 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4237	0.25344638026886734	No Hit
CCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	4011	0.239927644856839	RNA PCR Primer, Index 38 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCC	2451	0.146612480065847	No Hit
TCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	2026	0.12119007940163445	RNA PCR Primer, Index 27 (95% over 23bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1787	0.10689371761634786	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAATCG	95	0.0	40.263157	1
CGAAAGG	245	0.0	39.489796	2
GATTACG	40	3.4594814E-7	39.375	1
CGTTTTT	2470	0.0	38.62348	1
ACTTACG	35	6.249993E-6	38.57143	1
TTACGGG	525	0.0	38.142853	3
CGGTCTA	90	0.0	37.5	31
TATACGG	115	0.0	37.173916	2
CTACGAA	255	0.0	37.058823	11
TTAACGG	165	0.0	36.818184	2
GCTACGA	245	0.0	36.734695	10
AATTGCG	50	4.881258E-8	36.0	1
AATGCGG	290	0.0	35.689655	2
CAATCGG	145	0.0	35.689655	2
TAACGGG	495	0.0	35.454544	3
TACGGGA	480	0.0	35.15625	4
CGTTAGG	205	0.0	35.121952	2
TACGGGT	180	0.0	35.0	4
TCGTAAG	90	0.0	35.0	1
TATAGCG	90	0.0	35.0	1