##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549968_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1671754
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.29687920591187	33.0	31.0	34.0	30.0	34.0
2	32.45920871132954	34.0	31.0	34.0	31.0	34.0
3	32.46001086284226	34.0	31.0	34.0	30.0	34.0
4	35.99854404415961	37.0	35.0	37.0	35.0	37.0
5	35.87839000235681	37.0	35.0	37.0	35.0	37.0
6	35.83053248264996	37.0	35.0	37.0	35.0	37.0
7	36.064160755709274	37.0	35.0	37.0	35.0	37.0
8	36.04824992193828	37.0	35.0	37.0	35.0	37.0
9	37.61295621245709	39.0	38.0	39.0	35.0	39.0
10	37.382438444890816	39.0	37.0	39.0	34.0	39.0
11	37.24665172028899	39.0	37.0	39.0	34.0	39.0
12	37.02064119481694	39.0	37.0	39.0	33.0	39.0
13	36.90864026645069	39.0	37.0	39.0	33.0	39.0
14	37.79494291624246	40.0	37.0	41.0	33.0	41.0
15	37.95420199383402	40.0	37.0	41.0	33.0	41.0
16	38.080331197054115	40.0	37.0	41.0	33.0	41.0
17	38.16093755420953	40.0	37.0	41.0	33.0	41.0
18	38.12215613062688	40.0	37.0	41.0	33.0	41.0
19	38.08717730000945	40.0	37.0	41.0	34.0	41.0
20	38.01773347035509	40.0	36.0	41.0	33.0	41.0
21	38.00577058586371	40.0	36.0	41.0	33.0	41.0
22	37.96298737732944	40.0	36.0	41.0	34.0	41.0
23	37.740293727426405	40.0	36.0	41.0	33.0	41.0
24	37.529521089825415	39.0	35.0	41.0	33.0	41.0
25	37.52854786051058	39.0	35.0	41.0	33.0	41.0
26	37.6242724707104	39.0	35.0	41.0	33.0	41.0
27	37.702431697486595	40.0	36.0	41.0	33.0	41.0
28	37.65315291603908	40.0	35.0	41.0	33.0	41.0
29	37.638582590500754	40.0	35.0	41.0	33.0	41.0
30	37.5735610622137	40.0	35.0	41.0	33.0	41.0
31	37.38496273973324	39.0	35.0	41.0	32.0	41.0
32	37.312147600663735	39.0	35.0	41.0	32.0	41.0
33	37.26155522881955	39.0	35.0	41.0	32.0	41.0
34	37.10191212343443	39.0	35.0	41.0	32.0	41.0
35	37.06621907290187	39.0	35.0	41.0	32.0	41.0
36	36.99969134214723	39.0	35.0	41.0	31.0	41.0
37	36.914801460023426	39.0	35.0	41.0	31.0	41.0
38	36.83552304944388	39.0	35.0	41.0	31.0	41.0
39	36.77584919790831	39.0	35.0	41.0	31.0	41.0
40	36.683677742060134	39.0	35.0	41.0	31.0	41.0
41	36.58456926078837	39.0	35.0	41.0	31.0	41.0
42	36.561957680376416	39.0	35.0	41.0	31.0	41.0
43	36.48289461248485	39.0	35.0	41.0	31.0	41.0
44	36.39591710263591	39.0	35.0	41.0	31.0	41.0
45	36.3494246163012	38.0	35.0	40.0	31.0	41.0
46	36.26472495355178	38.0	35.0	40.0	31.0	41.0
47	36.14036754211445	38.0	35.0	40.0	30.0	41.0
48	36.04950548944402	38.0	35.0	40.0	30.0	41.0
49	36.0231427590423	38.0	35.0	40.0	30.0	41.0
50	35.92006718691865	38.0	35.0	40.0	30.0	41.0
51	34.89509640772506	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	15.0
10	12.0
11	20.0
12	18.0
13	26.0
14	26.0
15	64.0
16	120.0
17	239.0
18	424.0
19	758.0
20	1307.0
21	2069.0
22	3168.0
23	4491.0
24	6442.0
25	9085.0
26	11657.0
27	14194.0
28	17154.0
29	20434.0
30	25955.0
31	33207.0
32	43972.0
33	65458.0
34	119545.0
35	125371.0
36	127907.0
37	199471.0
38	345240.0
39	493604.0
40	298.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.075568534604976	21.165015905450204	25.32065124414238	11.438764315802445
2	32.2696401504049	23.858594027590183	25.936411696936272	17.93535412506864
3	31.790323217411178	23.377123667716663	27.293369718271947	17.539183396600215
4	29.254543431629294	25.02784500590398	26.754713911257276	18.96289765120945
5	25.423896099545747	29.545914051947836	25.04704639558212	19.983143452924292
6	27.184860930495752	33.26093432406921	24.35830869852861	15.195896046906423
7	80.804711698013	5.722492663394255	8.408533791454962	5.0642618471377965
8	81.18521026418959	4.6305257830996664	8.77324056051309	5.411023392197656
9	74.80616167211205	6.75918825377418	11.31488245280107	7.119767621312705
10	42.29192811861075	24.808135646751854	17.648589445576324	15.25134678906107
11	31.648914852304827	22.63490920314831	25.33949372934056	20.376682215206305
12	28.39401012349903	20.79241323783284	30.465786234099035	20.347790404569093
13	26.813813515624908	21.507530414163806	31.91073567043955	19.76792039977174
14	21.419180094679003	24.292808630934935	32.7744991188895	21.51351215549656
15	20.03817547318565	23.448725111469752	35.73253002535062	20.780569389993982
16	24.940092860552447	23.288294808925237	30.08684292066895	21.684769409853363
17	25.147898554452393	23.421149283925747	27.88281050920171	23.548141652420153
18	25.397157715788328	23.123318382967827	30.13828589613065	21.34123800511319
19	24.666069290098903	25.04345735078247	27.758629559133702	22.531843799984927
20	23.96602610192648	27.332729576241483	27.808995821155502	20.892248500676537
21	23.0852745080915	25.288888197665443	30.891985304057894	20.73385199018516
22	21.857342647303373	23.072353946812747	30.514118704067705	24.556184701816175
23	21.557777041358957	24.00155764544305	30.539600922145244	23.90106439105275
24	22.51611182027978	24.87889964671836	28.831335232336812	23.77365330066505
25	23.35576885115872	25.359891467285255	26.42141128419612	24.862928397359898
26	21.187507252861366	24.66876107369864	27.32764509610864	26.816086577331355
27	22.25118049665202	24.15050300462867	29.12156932180213	24.476747176917176
28	20.07831295752844	25.331837100434633	27.84692006120518	26.74292988083175
29	22.292693781501345	24.678930033964328	26.86812772692633	26.160248457608
30	25.213637891699374	24.00520650765603	27.252275155315914	23.528880445328678
31	23.675074203501232	26.36506328084156	26.31535501036636	23.64450750529085
32	24.77721004406151	24.95899516316396	25.419589245786163	24.844205546988373
33	25.678120106187873	24.35442056666232	26.234780954614134	23.732678372535673
34	21.050286106687945	25.484909861139858	28.783600936501426	24.681203095670774
35	21.73657128979503	25.442918036983908	27.26399936832812	25.556511304892943
36	25.031852772596924	26.210315632563162	26.336949096577605	22.420882498262305
37	22.68814670100984	28.403222005151473	26.953008636438135	21.95562265740055
38	22.70280196727509	25.948554631841763	27.71245051604482	23.63619288483832
39	23.188818450561506	23.7444624029612	27.788358813557494	25.278360332919796
40	23.175658619629445	23.564770893325214	29.85086322509173	23.408707261953612
41	21.446815739636335	24.49385495712886	27.869710495682977	26.189618807551827
42	22.872563786298702	23.572726609297778	29.480294349527504	24.074415254876016
43	23.18899790280149	22.489193984282377	29.584675735784096	24.737132377132042
44	22.260392378304463	23.130017933260515	28.166763770267632	26.442825918167383
45	21.67143012668132	22.482614068816346	27.88622010176138	27.95973570274095
46	22.51587255062647	23.270947759060245	28.948876449525468	25.264303240787818
47	20.61421716353004	24.25990905360478	30.695485101276866	24.43038868158832
48	20.876157616491422	24.403111941110954	29.6350419978059	25.08568844459173
49	23.527504644822145	22.57856119979375	29.889684726341315	24.00424942904279
50	20.58604316185276	22.804910291825234	29.59915154980936	27.009894996512646
51	20.579881968280024	22.773147245348298	28.228973880128294	28.41799690624338
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	806.0
1	1575.5
2	2345.0
3	4798.0
4	7251.0
5	4875.0
6	2499.0
7	2512.0
8	2525.0
9	2521.0
10	2517.0
11	2616.5
12	2716.0
13	2766.5
14	2817.0
15	3018.5
16	3220.0
17	3129.0
18	3038.0
19	3127.0
20	3216.0
21	3616.0
22	4016.0
23	4724.5
24	5433.0
25	6317.0
26	9555.0
27	11909.0
28	14196.5
29	16484.0
30	19094.0
31	21704.0
32	25082.0
33	28460.0
34	32462.5
35	36465.0
36	40651.0
37	44837.0
38	47319.0
39	49801.0
40	56487.5
41	63174.0
42	71482.0
43	79790.0
44	88917.0
45	98044.0
46	117052.0
47	136060.0
48	155564.5
49	175069.0
50	174789.0
51	174509.0
52	155145.0
53	135781.0
54	122761.5
55	109742.0
56	102437.0
57	95132.0
58	89270.5
59	83409.0
60	75105.5
61	66802.0
62	61881.0
63	56960.0
64	50582.5
65	44205.0
66	38177.0
67	32149.0
68	27996.5
69	23844.0
70	19907.5
71	15971.0
72	12822.0
73	9673.0
74	7668.5
75	4509.0
76	3354.0
77	2564.5
78	1775.0
79	1250.0
80	725.0
81	545.0
82	365.0
83	257.0
84	149.0
85	117.5
86	86.0
87	57.0
88	28.0
89	24.5
90	21.0
91	13.5
92	6.0
93	4.0
94	2.0
95	3.0
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1671754.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.97672990285446
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.58733922511364	20.45390263287282
2	11.559983448586301	6.699410361423067
3	4.293478699611355	3.7323291786689112
4	2.1009537426095672	2.4351507655195457
5	1.3089744771878284	1.8964899937600916
6	0.9140538295381591	1.5891774561118206
7	0.7067134548732484	1.433477143040267
8	0.5410808516318063	1.2543002954673055
9	0.4690216447047646	1.223164216548228
>10	7.220417027161201	49.47384254744298
>50	0.24021190915088095	4.2263386079996765
>100	0.05149216419939027	2.939082164754682
>500	0.0033490803369791147	0.6686427808349958
>1k	0.002721127773795531	1.6599615045257772
>5k	2.0931752106119467E-4	0.3147303510298252
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	5189	0.31039255775670344	RNA PCR Primer, Index 27 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCT	4575	0.27366466597358224	RNA PCR Primer, Index 38 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4237	0.25344638026886734	No Hit
CCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	4011	0.239927644856839	RNA PCR Primer, Index 38 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCC	2451	0.146612480065847	No Hit
TCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	2026	0.12119007940163445	RNA PCR Primer, Index 27 (95% over 23bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1787	0.10689371761634786	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.981741332755896E-5	0.0	0.0	0.2897555501586956	0.0
2	5.981741332755896E-5	0.0	0.0	1.0999824136804817	0.0
3	5.981741332755896E-5	0.0	0.0	1.631878852989136	0.0
4	5.981741332755896E-5	0.0	0.0	2.241537929623617	0.0
5	5.981741332755896E-5	0.0	0.0	3.74630477929169	0.0
6	5.981741332755896E-5	0.0	0.0	4.724798026503899	0.0
7	5.981741332755896E-5	0.0	0.0	5.741454783419091	0.0
8	5.981741332755896E-5	0.0	0.0	7.316387458920391	0.0
9	5.981741332755896E-5	0.0	0.0	7.960082643738254	0.0
10	5.981741332755896E-5	0.0	0.0	9.162053747142224	0.0
11	5.981741332755896E-5	0.0	0.0	10.927265614438488	0.0
12	5.981741332755896E-5	0.0	0.0	12.202513049168717	0.0
13	5.981741332755896E-5	0.0	0.0	12.720352396345396	0.0
14	5.981741332755896E-5	0.0	0.0	12.971645349734471	0.0
15	5.981741332755896E-5	0.0	0.0	13.367576808549583	0.0
16	1.1963482665511792E-4	0.0	0.0	14.137247465835284	0.0
17	1.1963482665511792E-4	0.0	0.0	15.074705967504789	0.0
18	1.1963482665511792E-4	0.0	0.0	16.0579846077832	0.0
19	1.1963482665511792E-4	0.0	0.0	16.681102602416384	0.0
20	1.1963482665511792E-4	0.0	0.0	17.344657168458994	0.0
21	1.1963482665511792E-4	0.0	0.0	18.149321012541318	0.0
22	1.1963482665511792E-4	0.0	0.0	19.003513674858862	0.0
23	1.1963482665511792E-4	0.0	0.0	19.789095764089694	0.0
24	1.1963482665511792E-4	0.0	0.0	20.42800555584135	0.0
25	1.1963482665511792E-4	0.0	0.0	20.981017542054634	0.0
26	1.1963482665511792E-4	0.0	0.0	21.50914548432365	0.0
27	1.1963482665511792E-4	0.0	0.0	22.14943107658184	0.0
28	1.1963482665511792E-4	0.0	0.0	22.716859059407067	0.0
29	1.1963482665511792E-4	0.0	0.0	23.350504918785898	0.0
30	2.990870666377948E-4	0.0	0.0	24.030329821253606	0.0
31	2.990870666377948E-4	0.0	0.0	24.576103900454253	0.0
32	2.990870666377948E-4	0.0	0.0	25.12283505826814	0.0
33	2.990870666377948E-4	0.0	0.0	25.66986530314867	0.0
34	2.990870666377948E-4	0.0	0.0	26.249496038292715	0.0
35	2.990870666377948E-4	0.0	0.0	26.847251449675014	0.0
36	2.990870666377948E-4	0.0	0.0	27.38614652634299	0.0
37	3.5890447996535376E-4	0.0	0.0	27.962367668927367	0.0
38	3.5890447996535376E-4	0.0	0.0	28.616889805557516	0.0
39	3.5890447996535376E-4	0.0	0.0	29.60973923196834	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAATCG	95	0.0	40.263157	1
CGAAAGG	245	0.0	39.489796	2
GATTACG	40	3.4594814E-7	39.375	1
CGTTTTT	2470	0.0	38.62348	1
ACTTACG	35	6.249993E-6	38.57143	1
TTACGGG	525	0.0	38.142853	3
CGGTCTA	90	0.0	37.5	31
TATACGG	115	0.0	37.173916	2
CTACGAA	255	0.0	37.058823	11
TTAACGG	165	0.0	36.818184	2
GCTACGA	245	0.0	36.734695	10
AATTGCG	50	4.881258E-8	36.0	1
AATGCGG	290	0.0	35.689655	2
CAATCGG	145	0.0	35.689655	2
TAACGGG	495	0.0	35.454544	3
TACGGGA	480	0.0	35.15625	4
CGTTAGG	205	0.0	35.121952	2
TACGGGT	180	0.0	35.0	4
TCGTAAG	90	0.0	35.0	1
TATAGCG	90	0.0	35.0	1
>>END_MODULE