##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549967_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	880387
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.21157627270734	33.0	31.0	34.0	30.0	34.0
2	32.36656720283239	34.0	31.0	34.0	30.0	34.0
3	32.37651623660958	34.0	31.0	34.0	30.0	34.0
4	35.9344095267195	37.0	35.0	37.0	35.0	37.0
5	35.81162602355555	37.0	35.0	37.0	35.0	37.0
6	35.76512261085182	37.0	35.0	37.0	35.0	37.0
7	35.9915752958642	37.0	35.0	37.0	35.0	37.0
8	35.96730301560564	37.0	35.0	37.0	35.0	37.0
9	37.49013672396344	39.0	37.0	39.0	35.0	39.0
10	37.29989197932273	39.0	37.0	39.0	34.0	39.0
11	37.22157414864145	39.0	37.0	39.0	34.0	39.0
12	37.05695336255533	39.0	37.0	39.0	34.0	39.0
13	36.92781697140008	39.0	37.0	39.0	33.0	39.0
14	37.99153894821255	40.0	37.0	41.0	33.0	41.0
15	38.11282083901739	40.0	37.0	41.0	33.0	41.0
16	38.14748968351418	40.0	37.0	41.0	33.0	41.0
17	38.17368838931061	40.0	37.0	41.0	34.0	41.0
18	38.13759857880682	40.0	37.0	41.0	34.0	41.0
19	38.108034307639706	40.0	37.0	41.0	34.0	41.0
20	38.04953957748127	40.0	36.0	41.0	34.0	41.0
21	38.02780141006171	40.0	36.0	41.0	34.0	41.0
22	37.91581656703245	40.0	36.0	41.0	33.0	41.0
23	37.67692616996843	39.0	36.0	41.0	33.0	41.0
24	37.47597477018629	39.0	35.0	41.0	33.0	41.0
25	37.49903394757079	39.0	35.0	41.0	33.0	41.0
26	37.59066978499228	39.0	36.0	41.0	33.0	41.0
27	37.654496261303265	39.0	36.0	41.0	33.0	41.0
28	37.663331012384326	39.0	36.0	41.0	33.0	41.0
29	37.67096629096068	40.0	36.0	41.0	33.0	41.0
30	37.60298028026311	40.0	36.0	41.0	33.0	41.0
31	37.47331344056648	39.0	35.0	41.0	33.0	41.0
32	37.374360366520634	39.0	35.0	41.0	33.0	41.0
33	37.32506045636748	39.0	35.0	41.0	33.0	41.0
34	37.26682583909122	39.0	35.0	41.0	33.0	41.0
35	37.19048100437649	39.0	35.0	41.0	32.0	41.0
36	37.09604980536968	39.0	35.0	41.0	32.0	41.0
37	36.996517440625546	39.0	35.0	41.0	32.0	41.0
38	36.92123918231414	39.0	35.0	41.0	31.0	41.0
39	36.8660157408049	39.0	35.0	41.0	31.0	41.0
40	36.78127687028545	39.0	35.0	41.0	31.0	41.0
41	36.71454258184185	39.0	35.0	41.0	31.0	41.0
42	36.65385336221457	39.0	35.0	41.0	31.0	41.0
43	36.53912313562104	39.0	35.0	41.0	31.0	41.0
44	36.453035994397915	39.0	35.0	40.0	31.0	41.0
45	36.399808266137505	39.0	35.0	40.0	31.0	41.0
46	36.293852589826976	38.0	35.0	40.0	31.0	41.0
47	36.16005574821073	38.0	35.0	40.0	30.0	41.0
48	36.09759798815748	38.0	35.0	40.0	30.0	41.0
49	36.08535450886939	38.0	35.0	40.0	30.0	41.0
50	35.983113108212635	38.0	35.0	40.0	30.0	41.0
51	34.980722114252025	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	4.0
10	8.0
11	8.0
12	5.0
13	12.0
14	17.0
15	25.0
16	64.0
17	114.0
18	199.0
19	359.0
20	683.0
21	1090.0
22	1669.0
23	2419.0
24	3295.0
25	4496.0
26	5705.0
27	7055.0
28	8561.0
29	10304.0
30	13154.0
31	16722.0
32	22235.0
33	31745.0
34	58810.0
35	76568.0
36	68945.0
37	107551.0
38	187543.0
39	250867.0
40	153.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.40679042284813	21.560631858489504	25.469026689399094	11.563551029263266
2	30.736142173839458	24.851230197628997	26.166788014816213	18.245839613715333
3	31.75069600073604	24.712654775683877	26.83853805201576	16.69811117156432
4	29.37435468720006	25.474365250736327	25.792407202741526	19.358872859322094
5	24.343044592889264	30.312124099969672	24.49343300162315	20.851398305517915
6	26.037072332962662	34.48858286185507	24.407334501758886	15.06701030342338
7	77.6330182067659	7.251129332895647	9.456295924405971	5.659556535932493
8	77.41288774141371	6.0847104739165845	9.604071845676957	6.898329938992738
9	70.77398916612808	8.150279365778914	12.781083773386023	8.294647694706986
10	39.59974420340146	24.852706820977595	19.56298764066257	15.984561334958377
11	30.678894622478524	23.7134351143304	24.308287150991553	21.29938311219952
12	27.196335248021608	21.54325313754065	30.630847570443454	20.62956404399429
13	24.276823715025326	24.315329508500238	31.670049648620434	19.737797127854
14	19.385679252419674	28.44419556399629	30.353923899376067	21.816201284207967
15	19.096829008152095	24.78057944971927	35.33207555313743	20.790515988991203
16	21.091747152104702	24.894733793206854	31.11165885002845	22.901860204659997
17	21.246565430884374	24.87031271474931	27.54175152518154	26.341370329184777
18	21.903322061775103	25.629751461573147	30.414124697434197	22.05280177921755
19	23.426629425468573	26.23073716445154	27.60615502046259	22.736478389617293
20	23.87461423215018	27.001534552418427	27.309694486629176	21.81415672880222
21	22.772826041275028	27.01084863815572	28.94465729275875	21.2716680278105
22	19.873532889513363	26.45518391343807	27.73371256049896	25.937570636549605
23	21.157286511500057	25.865102506056996	28.289831630862338	24.68777935158061
24	22.53622554626545	24.41653500108475	28.729638215920954	24.317601236728848
25	21.12911708146531	25.813079929621857	26.65259709650415	26.405205892408677
26	18.839215027027887	27.71712894443012	27.0794548306597	26.3642011978823
27	21.58028230766697	27.115802482317434	27.285273408171633	24.018641801843962
28	18.3330739776939	26.576039855200044	28.336629232371674	26.754256934734382
29	19.775507816448904	24.95834218360789	28.162046918003103	27.1041030819401
30	21.4590856066707	26.31990249742443	27.309694486629176	24.911317409275693
31	21.835965319796863	26.162926076827574	24.31782840955171	27.68328019382385
32	21.074822776801565	26.49368970691298	25.147009212993833	27.28447830329162
33	22.33767649908506	25.975962843613093	26.02128382177383	25.665076835528012
34	19.438837692969116	23.379377478313515	27.46280896923739	29.71897585947998
35	19.92430601542276	23.00658687599885	27.20723954351893	29.86186756505946
36	22.46727859452718	25.843407501473788	26.50845594039894	25.1808579636001
37	20.118311606145934	26.61454564867496	27.224163918822065	26.042978826357043
38	20.14591310412353	25.771620889449753	27.083543941471195	26.998922064955526
39	22.910152012694418	22.709217650873992	28.93114050979853	25.44948982663306
40	21.381051742018	22.45149008333835	29.50940893039084	26.658049244252812
41	19.64454268406962	23.927091154231036	24.66381261876879	31.76455354293055
42	21.698412175554612	23.819751995429282	25.65065136127635	28.831184467739757
43	22.8574479177907	22.10073524484119	28.41011964056716	26.631697196800953
44	20.63592488303439	23.151068791338357	28.01654272496073	28.196463600666526
45	20.39341789463043	22.288152823701395	28.385925734932478	28.9325035467357
46	22.134811168270318	22.662987981421807	28.304711450759722	26.897489399548153
47	19.19360463069082	22.498969203316268	30.410944277914144	27.896481888078767
48	19.76460352095158	21.799276908904833	30.024068960582106	28.41205060956148
49	22.138900279081813	20.354571341921222	31.438674128536654	26.06785425046031
50	20.09320900921981	20.67227253469213	30.447632688806177	28.786885767281888
51	19.851383539284427	20.982136265074335	27.325028652172286	31.841451543468953
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	706.0
1	1063.5
2	1421.0
3	2125.0
4	2829.0
5	2136.0
6	1443.0
7	1440.0
8	1437.0
9	1451.5
10	1466.0
11	1562.5
12	1659.0
13	1748.5
14	1838.0
15	1970.0
16	2102.0
17	2100.5
18	2099.0
19	2273.0
20	2447.0
21	2473.0
22	2499.0
23	2724.0
24	2949.0
25	4030.5
26	5607.5
27	6103.0
28	7995.0
29	9887.0
30	11271.0
31	12655.0
32	13294.5
33	13934.0
34	15202.5
35	16471.0
36	17852.0
37	19233.0
38	21188.0
39	23143.0
40	27032.5
41	30922.0
42	36066.0
43	41210.0
44	45770.5
45	50331.0
46	55834.0
47	61337.0
48	73202.5
49	85068.0
50	97375.5
51	109683.0
52	103593.0
53	97503.0
54	81642.5
55	65782.0
56	57036.0
57	48290.0
58	42792.5
59	37295.0
60	34784.0
61	32273.0
62	29636.0
63	26999.0
64	24226.5
65	21454.0
66	17785.5
67	14117.0
68	11721.0
69	9325.0
70	7925.5
71	6526.0
72	5242.0
73	3958.0
74	3479.0
75	2448.0
76	1896.0
77	1504.5
78	1113.0
79	800.5
80	488.0
81	391.0
82	294.0
83	169.0
84	44.0
85	34.0
86	24.0
87	18.5
88	13.0
89	9.0
90	5.0
91	3.5
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	880387.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.610323459698577
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.51285225365913	19.88785187583425
2	12.576580622430159	7.196400792494082
3	4.96335167369387	4.260092904858538
4	2.4541082789598705	2.808513266646643
5	1.4166372139029548	2.026522445740487
6	0.967510745317811	1.6608477224565976
7	0.6742048611545979	1.350245340901395
8	0.5130589306258024	1.1743025567273007
9	0.4446927059152353	1.1450521940763145
>10	5.861390872217449	41.01769106803346
>50	0.5171621689663609	8.990833089507284
>100	0.08920745894206493	4.801916925111241
>500	0.005625695608959048	1.194041112435485
>1k	0.003214683205119456	1.9057981437360907
>5k	4.01835400639932E-4	0.5798905614408422
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC	5044	0.5729298592550776	No Hit
CTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGCT	4481	0.5089807096197467	No Hit
CCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC	3832	0.4352631286013991	No Hit
TCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC	1977	0.22456033539795567	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCC	1419	0.16117911781977698	No Hit
ACTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC	1416	0.1608383585854857	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1298	0.14743516203669524	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1121	0.12733036721350952	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1033	0.11733476300763188	No Hit
CGCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTG	955	0.1084750229160585	No Hit
GCCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTG	950	0.10790709085890637	No Hit
AGCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTG	948	0.10767991803604551	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	897	0.1018870110530937	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.4075923429128325E-4	0.0	0.0	0.5479408487403835	0.0
2	3.4075923429128325E-4	0.0	0.0	2.0505754855535123	0.0
3	3.4075923429128325E-4	0.0	0.0	2.910538206493281	0.0
4	3.4075923429128325E-4	0.0	0.0	3.8437641628056753	0.0
5	3.4075923429128325E-4	0.0	0.0	6.054155729241799	0.0
6	3.4075923429128325E-4	0.0	0.0	7.270325436427389	0.0
7	3.4075923429128325E-4	0.0	0.0	8.511029808481952	0.0
8	3.4075923429128325E-4	0.0	0.0	10.564331367909794	0.0
9	3.4075923429128325E-4	0.0	0.0	11.304460424790461	0.0
10	3.4075923429128325E-4	0.0	0.0	12.79619076610627	0.0
11	3.4075923429128325E-4	0.0	0.0	14.989090025182108	0.0
12	3.4075923429128325E-4	0.0	0.0	16.722418663610433	0.0
13	3.4075923429128325E-4	0.0	0.0	17.41972564338183	0.0
14	3.4075923429128325E-4	0.0	0.0	17.69574062315777	0.0
15	3.4075923429128325E-4	0.0	0.0	18.2132403136348	0.0
16	3.4075923429128325E-4	0.0	0.0	19.273683050749273	0.0
17	3.4075923429128325E-4	0.0	0.0	20.58004036861062	0.0
18	3.4075923429128325E-4	0.0	0.0	22.061320760074832	0.0
19	3.4075923429128325E-4	0.0	0.0	22.877098366968163	0.0
20	3.4075923429128325E-4	0.0	0.0	23.710936213278934	0.0
21	3.4075923429128325E-4	0.0	0.0	24.66858324804887	0.0
22	3.4075923429128325E-4	0.0	0.0	25.62986504798458	0.0
23	3.4075923429128325E-4	0.0	0.0	26.486079417347145	0.0
24	3.4075923429128325E-4	0.0	0.0	27.238702979485158	0.0
25	3.4075923429128325E-4	0.0	0.0	27.888871598512928	0.0
26	3.4075923429128325E-4	0.0	0.0	28.477703555368265	0.0
27	3.4075923429128325E-4	0.0	0.0	29.059265981892054	0.0
28	3.4075923429128325E-4	0.0	0.0	29.677743992130733	0.0
29	3.4075923429128325E-4	0.0	0.0	30.297130693660854	0.0
30	3.4075923429128325E-4	0.0	0.0	30.99205235879221	0.0
31	4.54345645721711E-4	0.0	0.0	31.61507382548811	0.0
32	4.54345645721711E-4	0.0	0.0	32.229462724915294	0.0
33	4.54345645721711E-4	0.0	0.0	32.808753423210476	0.0
34	4.54345645721711E-4	0.0	0.0	33.382364800934134	0.0
35	6.815184685825665E-4	0.0	0.0	33.959724530235	0.0
36	6.815184685825665E-4	0.0	0.0	34.5136854587812	0.0
37	6.815184685825665E-4	0.0	0.0	35.06912301067599	0.0
38	6.815184685825665E-4	0.0	0.0	35.6339882347195	0.0
39	6.815184685825665E-4	0.0	0.0	36.25769121988398	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATATGG	70	0.0	45.000004	2
GTTAGCG	65	0.0	45.000004	1
CGAACTC	25	3.8900587E-5	45.0	26
GTCGAAA	25	3.8900587E-5	45.0	15
ACCGAAT	20	7.032387E-4	45.0	25
CACGAAC	40	6.8121153E-9	45.0	24
CGTAGAC	20	7.032387E-4	45.0	16
GCGATAC	40	6.8121153E-9	45.0	9
ACCACGG	50	2.1827873E-11	45.0	2
GTATACG	20	7.032387E-4	45.0	1
TATAGCG	50	2.1827873E-11	45.0	1
ATGCGAA	45	3.8380676E-10	45.0	26
TTACGCA	20	7.032387E-4	45.0	41
CGATACA	20	7.032387E-4	45.0	10
TTAGCCG	30	2.1649212E-6	44.999996	1
CGTAAGG	180	0.0	42.5	2
CGTATGG	75	0.0	42.000004	2
TCGATAG	55	6.002665E-11	40.909092	1
GCGTAAG	135	0.0	40.000004	1
CGGGTAT	80	0.0	39.375	6
>>END_MODULE