FastQCFastQC Report
Sat 18 Jun 2016
SRR3549966_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549966_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences874194
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGCT42960.49142410037131345No Hit
GCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC36520.41775624174954296No Hit
CCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC29690.33962713081993245No Hit
TCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC14460.16540950864453427No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCC12370.1415017719179038No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11490.1314353564540594No Hit
ACTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC11370.13006266343626244No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCG302.164903E-645.0000041
ATGCGAA302.164903E-645.00000426
AATCCGG207.0323586E-445.02
CGAACTA207.0323586E-445.035
TAACGCG207.0323586E-445.01
CCCGTTG207.0323586E-445.028
TCAATCG207.0323586E-445.038
TCGGACG253.890037E-545.01
TGCGATA207.0323586E-445.033
AATTCGC207.0323586E-445.013
CTCGAAT207.0323586E-445.025
TGCGTTG207.0323586E-445.01
TACGAAT1000.042.7512
TTAACGG556.002665E-1140.9090922
GCTACGA1100.040.90909210
CGAATAT1000.040.514
TTACGAG501.0804797E-940.51
CATACGA1900.040.26315718
ATACGAA1900.040.26315719
TCGTAAG451.9274012E-840.01