##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549966_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	874194
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.30330910530157	33.0	31.0	34.0	30.0	34.0
2	32.460982344879966	34.0	31.0	34.0	31.0	34.0
3	32.47381702459637	34.0	31.0	34.0	31.0	34.0
4	36.01760707577494	37.0	35.0	37.0	35.0	37.0
5	35.88459541017211	37.0	35.0	37.0	35.0	37.0
6	35.83617709570187	37.0	35.0	37.0	35.0	37.0
7	36.0273863696159	37.0	35.0	37.0	35.0	37.0
8	35.98991528196259	37.0	35.0	37.0	35.0	37.0
9	37.53352573913799	39.0	38.0	39.0	35.0	39.0
10	37.3791515384457	39.0	37.0	39.0	34.0	39.0
11	37.270104805111906	39.0	37.0	39.0	34.0	39.0
12	37.05487225947559	39.0	37.0	39.0	34.0	39.0
13	36.96832396470349	39.0	37.0	39.0	33.0	39.0
14	38.062280226128294	40.0	37.0	41.0	33.0	41.0
15	38.157675527400094	40.0	37.0	41.0	33.0	41.0
16	38.174344596279546	40.0	37.0	41.0	34.0	41.0
17	38.197941189255474	40.0	37.0	41.0	34.0	41.0
18	38.17173876736743	40.0	37.0	41.0	34.0	41.0
19	38.130726131728196	40.0	37.0	41.0	34.0	41.0
20	38.09613541159056	40.0	36.0	41.0	34.0	41.0
21	38.06661907997538	40.0	36.0	41.0	34.0	41.0
22	37.96692381782533	40.0	36.0	41.0	34.0	41.0
23	37.75136868932983	40.0	36.0	41.0	33.0	41.0
24	37.564163103384374	39.0	35.0	41.0	33.0	41.0
25	37.5634962033599	39.0	35.0	41.0	33.0	41.0
26	37.61890610093412	39.0	35.0	41.0	33.0	41.0
27	37.699587276965985	39.0	35.0	41.0	33.0	41.0
28	37.67662898624333	40.0	36.0	41.0	33.0	41.0
29	37.67345234581798	40.0	36.0	41.0	33.0	41.0
30	37.62716284943617	40.0	36.0	41.0	33.0	41.0
31	37.482987757866105	40.0	35.0	41.0	33.0	41.0
32	37.3568738746777	39.0	35.0	41.0	33.0	41.0
33	37.32205322845959	39.0	35.0	41.0	33.0	41.0
34	37.23379249914779	39.0	35.0	41.0	33.0	41.0
35	37.165041169351426	39.0	35.0	41.0	32.0	41.0
36	37.11070654797448	39.0	35.0	41.0	32.0	41.0
37	37.04066374283054	39.0	35.0	41.0	32.0	41.0
38	36.947083828074774	39.0	35.0	41.0	32.0	41.0
39	36.894540571086054	39.0	35.0	41.0	32.0	41.0
40	36.82348197310895	39.0	35.0	41.0	31.0	41.0
41	36.77346790300551	39.0	35.0	41.0	31.0	41.0
42	36.730532353230515	39.0	35.0	41.0	31.0	41.0
43	36.61902392375148	39.0	35.0	41.0	31.0	41.0
44	36.526379728069514	39.0	35.0	41.0	31.0	41.0
45	36.45927219816196	39.0	35.0	40.0	31.0	41.0
46	36.35781531330574	38.0	35.0	40.0	31.0	41.0
47	36.243354449927594	38.0	35.0	40.0	31.0	41.0
48	36.19027813048362	38.0	35.0	40.0	31.0	41.0
49	36.145041032082126	38.0	35.0	40.0	31.0	41.0
50	36.04829362818779	38.0	35.0	40.0	31.0	41.0
51	35.08065143434981	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	6.0
10	3.0
11	4.0
12	11.0
13	14.0
14	21.0
15	33.0
16	69.0
17	107.0
18	211.0
19	348.0
20	607.0
21	991.0
22	1554.0
23	2221.0
24	3123.0
25	4348.0
26	5532.0
27	6777.0
28	8074.0
29	9716.0
30	12324.0
31	15998.0
32	20891.0
33	30942.0
34	60553.0
35	79809.0
36	66228.0
37	103347.0
38	181804.0
39	258364.0
40	161.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.29576730107962	20.694948718476677	25.00383210134135	11.00545187910235
2	31.795459589061466	24.47751872010103	25.784665646298187	17.942356044539313
3	32.083153167374746	23.921006092469177	26.382931019888034	17.612909720268043
4	29.391302159474897	25.33350720778225	25.62611960274264	19.649071030000208
5	25.132522071759816	29.870143240516406	24.424784430000663	20.572550257723112
6	27.074539518688074	34.20934026085743	23.67472208685944	15.04139813359506
7	78.65027671203417	6.593273346648456	8.915984323845736	5.8404656174716365
8	78.70987446722351	5.767712887528398	9.268880820504373	6.253531824743706
9	72.65355287270332	7.909113995291663	11.71296073869187	7.724372393313154
10	41.59328478575694	24.375710654614423	18.227304236816998	15.803700322811643
11	32.472997984429085	24.098197882849803	23.332807134343177	20.095996998377935
12	30.699821778689856	21.106184668391684	28.639180776806977	19.55481277611148
13	26.428573062729782	23.264973221047043	29.399881490836133	20.906572225387045
14	20.416177644779076	28.142609077618925	28.6072656641432	22.833947613458797
15	19.927041366104092	25.165924268526208	34.1624399160827	20.744594449287
16	22.526349986387462	24.506116491305132	31.568393285700886	21.39914023660652
17	21.594520209472954	24.817717806345044	28.36807390579208	25.21968807838992
18	23.18958949615303	25.12657373534936	29.207704468344552	22.476132300153058
19	24.177814077882026	27.20826269683846	26.909816356552437	21.70410686872708
20	27.011853204208673	26.089174714079483	26.857539630791333	20.041432450920503
21	24.609182858724722	27.120982299123536	27.407188793334203	20.862646048817542
22	21.511929846235503	26.113425624060564	26.857882804045786	25.51676172565815
23	23.21315405962521	26.38979448497702	27.371727557041115	23.02532389835666
24	24.391496624319085	23.346305282351516	28.4267565323029	23.8354415610265
25	22.941589624271042	24.863245458101975	26.122348128676244	26.072816788950735
26	20.92258697726134	26.642141218082028	28.53165315707955	23.903618647577083
27	22.206054948901503	25.393676918395684	28.8999924501884	23.500275682514406
28	19.216672729394162	27.06973509312578	28.92001089003127	24.79358128744878
29	21.651029405372263	26.1659311319913	27.769808532202234	24.413230930434203
30	22.89160072020627	25.610104850868343	27.098218473245065	24.400075955680318
31	22.74300670102975	26.32024470540864	24.679990940226084	26.256757653335526
32	23.30043445734013	26.696133810115374	25.290152986636834	24.71327874590766
33	23.61020551502298	25.20172867807375	25.734676742233418	25.453389064669857
34	20.52999677417141	24.869994532106148	27.58369423720593	27.016314456516515
35	20.803277075797823	24.66866622282926	27.737321464114373	26.790735237258552
36	21.609848614838352	26.717296160806413	26.98725912097315	24.68559610338209
37	21.572328339018572	27.654044754368023	26.411757573261767	24.361869333351635
38	21.338512961653823	27.619498646753467	24.207326977764662	26.834661413828055
39	23.43667423935648	24.964367177079687	25.172558951445563	26.42639963211827
40	22.634335170454158	22.7726339919972	27.938306600136816	26.65472423741183
41	19.67801197445876	24.05461487953475	26.82127765690453	29.44609548910196
42	22.053800415010855	24.019954380835376	27.004303392610794	26.921941811542975
43	22.844814766516357	23.64017597924488	27.72942847926204	25.785580774976722
44	20.94683788724242	24.115928500996347	26.773004619112008	28.164228992649225
45	21.217487193918057	23.07085155011359	26.69304525082533	29.018616005143024
46	21.367110732857924	24.13011299551358	27.342214657158483	27.160561614470012
47	19.94911884547366	23.472021084564755	29.641246679798762	26.937613390162824
48	20.367675824816917	21.958398250273966	29.780003065681072	27.89392285922804
49	21.78761236064306	21.694841190856952	29.907663516336193	26.6098829321638
50	19.49681649610956	22.036641752288393	29.57238324673928	28.894158504862766
51	19.387573010109886	21.55356820110868	27.727712612989798	31.33114617579164
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	460.0
1	1020.5
2	1581.0
3	2156.5
4	2732.0
5	2095.5
6	1459.0
7	1435.5
8	1412.0
9	1422.5
10	1433.0
11	1529.5
12	1626.0
13	1707.0
14	1788.0
15	1688.0
16	1588.0
17	1648.0
18	1708.0
19	1885.0
20	2062.0
21	2293.0
22	2524.0
23	2960.0
24	3396.0
25	3977.0
26	5741.5
27	6925.0
28	7616.5
29	8308.0
30	10270.0
31	12232.0
32	13248.5
33	14265.0
34	16572.5
35	18880.0
36	20172.5
37	21465.0
38	23266.5
39	25068.0
40	27507.0
41	29946.0
42	34308.5
43	38671.0
44	43933.0
45	49195.0
46	53147.5
47	57100.0
48	66088.5
49	75077.0
50	85272.0
51	95467.0
52	92987.5
53	90508.0
54	78151.0
55	65794.0
56	58289.5
57	50785.0
58	48075.5
59	45366.0
60	43058.5
61	40751.0
62	37298.0
63	33845.0
64	28522.5
65	23200.0
66	19509.0
67	15818.0
68	13128.5
69	10439.0
70	8530.0
71	6621.0
72	5539.5
73	4458.0
74	3526.5
75	2078.0
76	1561.0
77	1242.0
78	923.0
79	625.0
80	327.0
81	243.5
82	160.0
83	114.0
84	68.0
85	45.5
86	23.0
87	17.5
88	12.0
89	8.5
90	5.0
91	5.0
92	5.0
93	3.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	874194.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.38655235710031
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.4950041172889	20.71605129406712
2	11.682867869730979	6.866384166698687
3	4.400150369831964	3.879157476665439
4	2.1249023980166513	2.497742222921773
5	1.2915684412474915	1.8977371810748922
6	0.9168416304571082	1.6165688745958213
7	0.6736142969176824	1.385664126340395
8	0.5239871211124798	1.2318539975214517
9	0.48304797972175484	1.277560327237639
>10	7.099198924520496	48.286173928610864
>50	0.2427830317497942	4.419357941024384
>100	0.06049805011326102	3.516150920597073
>500	0.0027678846457047514	0.5636764083217579
>1k	0.0027678846457047514	1.8459211343227058
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGCT	4296	0.49142410037131345	No Hit
GCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC	3652	0.41775624174954296	No Hit
CCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC	2969	0.33962713081993245	No Hit
TCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC	1446	0.16540950864453427	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCC	1237	0.1415017719179038	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1149	0.1314353564540594	No Hit
ACTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC	1137	0.13006266343626244	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5215089556780302	0.0
2	0.0	0.0	0.0	1.640368156267373	0.0
3	0.0	0.0	0.0	2.3098991756978426	0.0
4	0.0	0.0	0.0	3.033537178246476	0.0
5	0.0	0.0	0.0	4.689462522048881	0.0
6	0.0	0.0	0.0	5.642569040739241	0.0
7	0.0	0.0	0.0	6.62759067209338	0.0
8	0.0	0.0	0.0	8.227006819996477	0.0
9	0.0	0.0	0.0	8.828703926130814	0.0
10	1.1439108481641375E-4	0.0	0.0	10.091695893588838	0.0
11	2.287821696328275E-4	0.0	0.0	11.970455070613617	0.0
12	2.287821696328275E-4	0.0	0.0	13.367170216222029	0.0
13	2.287821696328275E-4	0.0	0.0	13.93329169497846	0.0
14	2.287821696328275E-4	0.0	0.0	14.183121824217508	0.0
15	2.287821696328275E-4	0.0	0.0	14.643202767349123	0.0
16	2.287821696328275E-4	0.0	0.0	15.558102663710802	0.0
17	2.287821696328275E-4	0.0	0.0	16.629947128440598	0.0
18	2.287821696328275E-4	0.0	0.0	17.85667712201182	0.0
19	2.287821696328275E-4	0.0	0.0	18.59186862412691	0.0
20	2.287821696328275E-4	0.0	0.0	19.313676369318483	0.0
21	2.287821696328275E-4	0.0	0.0	20.17652832208869	0.0
22	2.287821696328275E-4	0.0	0.0	21.00906663738255	0.0
23	2.287821696328275E-4	0.0	0.0	21.8074020183163	0.0
24	2.287821696328275E-4	0.0	0.0	22.456228251395	0.0
25	2.287821696328275E-4	0.0	0.0	23.051862630034066	0.0
26	2.287821696328275E-4	0.0	0.0	23.570626199676504	0.0
27	2.287821696328275E-4	0.0	0.0	24.104603783599522	0.0
28	2.287821696328275E-4	0.0	0.0	24.65127877793716	0.0
29	2.287821696328275E-4	0.0	0.0	25.207219450144933	0.0
30	2.287821696328275E-4	0.0	0.0	25.824473743814302	0.0
31	2.287821696328275E-4	0.0	0.0	26.38945131172257	0.0
32	2.287821696328275E-4	0.0	0.0	26.971701933438116	0.0
33	2.287821696328275E-4	0.0	0.0	27.50007435420513	0.0
34	2.287821696328275E-4	0.0	0.0	28.03725488850301	0.0
35	2.287821696328275E-4	0.0	0.0	28.59159408552335	0.0
36	2.287821696328275E-4	0.0	0.0	29.1418152034903	0.0
37	2.287821696328275E-4	0.0	0.0	29.68482968311382	0.0
38	2.287821696328275E-4	0.0	0.0	30.23173345962109	0.0
39	2.287821696328275E-4	0.0	0.0	30.825537580903095	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATGCG	30	2.164903E-6	45.000004	1
ATGCGAA	30	2.164903E-6	45.000004	26
AATCCGG	20	7.0323586E-4	45.0	2
CGAACTA	20	7.0323586E-4	45.0	35
TAACGCG	20	7.0323586E-4	45.0	1
CCCGTTG	20	7.0323586E-4	45.0	28
TCAATCG	20	7.0323586E-4	45.0	38
TCGGACG	25	3.890037E-5	45.0	1
TGCGATA	20	7.0323586E-4	45.0	33
AATTCGC	20	7.0323586E-4	45.0	13
CTCGAAT	20	7.0323586E-4	45.0	25
TGCGTTG	20	7.0323586E-4	45.0	1
TACGAAT	100	0.0	42.75	12
TTAACGG	55	6.002665E-11	40.909092	2
GCTACGA	110	0.0	40.909092	10
CGAATAT	100	0.0	40.5	14
TTACGAG	50	1.0804797E-9	40.5	1
CATACGA	190	0.0	40.263157	18
ATACGAA	190	0.0	40.263157	19
TCGTAAG	45	1.9274012E-8	40.0	1
>>END_MODULE