SRR3549962_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549962_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	549515
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGC	4889	0.8896936389361528	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCG	4411	0.8027078423700901	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTC	3617	0.6582167911703957	TruSeq Adapter, Index 22 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC	3062	0.5572186382537327	TruSeq Adapter, Index 22 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCT	2917	0.5308317334376678	Illumina Single End Adapter 2 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC	2321	0.4223724557109451	TruSeq Adapter, Index 22 (95% over 24bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGACGTGCTCGTA	960	0.1746995077477412	No Hit
TCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC	956	0.1739715931321256	TruSeq Adapter, Index 22 (95% over 24bp)
GAATGACTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCT	919	0.16723838293768142	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCC	846	0.15395394120269693	No Hit
GAACTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCT	788	0.14339917927627088	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTC	766	0.13939564889038517	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	756	0.1375758623513462	No Hit
ACTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC	732	0.13320837465765265	Illumina Single End Adapter 2 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGACGTGCT	710	0.12920484427176693	No Hit
CGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTG	630	0.11464655195945515	Illumina Single End Adapter 2 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTG	572	0.10409179003302911	TruSeq Adapter, Index 22 (95% over 23bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTAC	35	1.2103737E-7	45.000004	8
TACGGGT	70	0.0	45.000004	4
GTATACG	35	1.2103737E-7	45.000004	1
CGGGTTA	25	3.8881393E-5	45.0	6
AATCCGT	20	7.030069E-4	45.0	42
TCGTTCA	25	3.8881393E-5	45.0	16
ACACGAC	25	3.8881393E-5	45.0	26
ATTAGCG	20	7.030069E-4	45.0	1
GGCGTAT	20	7.030069E-4	45.0	6
ATTACGG	20	7.030069E-4	45.0	2
AGGCGTA	20	7.030069E-4	45.0	5
CACGACC	25	3.8881393E-5	45.0	27
AAACGCG	20	7.030069E-4	45.0	1
TCGGATC	20	7.030069E-4	45.0	40
TGATTAC	20	7.030069E-4	45.0	11
AACGAGC	40	6.8030204E-9	45.0	15
ATGCGTC	20	7.030069E-4	45.0	10
AACCTAG	25	3.8881393E-5	45.0	21
CACGTAG	20	7.030069E-4	45.0	1
CACAACG	40	6.8030204E-9	45.0	12

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content