##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549962_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	549515
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.24796229402291	33.0	31.0	34.0	30.0	34.0
2	32.40426012028789	34.0	31.0	34.0	30.0	34.0
3	32.41876927836365	34.0	31.0	34.0	30.0	34.0
4	35.97811160750844	37.0	35.0	37.0	35.0	37.0
5	35.85164372219139	37.0	35.0	37.0	35.0	37.0
6	35.78291402418496	37.0	35.0	37.0	35.0	37.0
7	35.97756931111981	37.0	35.0	37.0	35.0	37.0
8	35.867115547346295	37.0	35.0	37.0	35.0	37.0
9	37.45486474436549	39.0	37.0	39.0	35.0	39.0
10	37.32938500313913	39.0	37.0	39.0	34.0	39.0
11	37.23528566099196	39.0	37.0	39.0	34.0	39.0
12	37.07232377642102	39.0	37.0	39.0	34.0	39.0
13	36.939850595525144	39.0	37.0	39.0	33.0	39.0
14	38.0333293904625	40.0	37.0	41.0	33.0	41.0
15	38.101984477220824	40.0	37.0	41.0	33.0	41.0
16	38.17808067113727	40.0	37.0	41.0	34.0	41.0
17	38.162142980628374	40.0	37.0	41.0	34.0	41.0
18	38.14981210703984	40.0	37.0	41.0	34.0	41.0
19	38.095438705039896	40.0	37.0	41.0	34.0	41.0
20	38.02048897664304	40.0	36.0	41.0	34.0	41.0
21	38.0295442344613	40.0	36.0	41.0	34.0	41.0
22	37.93907354667297	40.0	36.0	41.0	33.0	41.0
23	37.664433181987754	39.0	36.0	41.0	33.0	41.0
24	37.463583341674024	39.0	35.0	41.0	32.0	41.0
25	37.47589055803754	39.0	35.0	41.0	33.0	41.0
26	37.56061435993558	39.0	35.0	41.0	33.0	41.0
27	37.600169240148134	39.0	35.0	41.0	33.0	41.0
28	37.59579629309482	39.0	36.0	41.0	33.0	41.0
29	37.600238392036616	39.0	36.0	41.0	33.0	41.0
30	37.537630455947514	39.0	36.0	41.0	33.0	41.0
31	37.35906026223124	39.0	35.0	41.0	33.0	41.0
32	37.24648280756667	39.0	35.0	41.0	33.0	41.0
33	37.17902514035104	39.0	35.0	41.0	32.0	41.0
34	37.041252741053476	39.0	35.0	41.0	32.0	41.0
35	37.01001974468395	39.0	35.0	41.0	32.0	41.0
36	36.91529803554043	39.0	35.0	41.0	32.0	41.0
37	36.821326078450994	39.0	35.0	41.0	31.0	41.0
38	36.72902832497748	39.0	35.0	41.0	31.0	41.0
39	36.70330200267509	39.0	35.0	41.0	31.0	41.0
40	36.583003193725375	39.0	35.0	41.0	31.0	41.0
41	36.47341200877137	39.0	35.0	41.0	31.0	41.0
42	36.471990755484384	39.0	35.0	40.0	31.0	41.0
43	36.397223005741424	39.0	35.0	40.0	31.0	41.0
44	36.30378424610793	39.0	35.0	40.0	31.0	41.0
45	36.239341965187485	38.0	35.0	40.0	30.0	41.0
46	36.09365895380472	38.0	35.0	40.0	30.0	41.0
47	35.905039898819865	38.0	35.0	40.0	30.0	41.0
48	35.8788531705231	38.0	35.0	40.0	30.0	41.0
49	35.8858083946753	38.0	35.0	40.0	30.0	41.0
50	35.77633185627326	38.0	35.0	40.0	29.0	41.0
51	34.677306351964916	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	2.0
10	7.0
11	2.0
12	4.0
13	9.0
14	15.0
15	20.0
16	46.0
17	89.0
18	152.0
19	270.0
20	519.0
21	836.0
22	1329.0
23	1844.0
24	2404.0
25	3032.0
26	3824.0
27	4528.0
28	5314.0
29	6476.0
30	8160.0
31	10484.0
32	14160.0
33	20312.0
34	36906.0
35	48799.0
36	44578.0
37	67449.0
38	115421.0
39	152421.0
40	101.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.804418441716784	20.351036823380618	23.58443354594506	11.260111188957534
2	30.359316852133244	28.127166683348044	24.301065485018608	17.212450979500105
3	30.854844726713555	27.498976370071794	25.566363065612403	16.079815837602247
4	28.522242340973403	23.93001100970856	28.459823662684368	19.087922986633668
5	27.133563233032763	28.39376541131725	23.87159586180541	20.601075493844572
6	24.68121889302385	35.92731772563078	24.169676896899993	15.221786484445374
7	75.42196300373966	6.878975096221213	11.914870385703757	5.784191514335369
8	74.37067232013685	8.534434910784965	10.365140169058169	6.729752600020017
9	68.25891922877446	7.773764137466675	12.465355813763047	11.501960819995814
10	41.5737513989609	22.810842288199595	19.511387314268035	16.10401899857147
11	31.806047150669226	23.35332065548711	24.207892414219813	20.632739779623847
12	28.25709944223543	20.300992693557045	29.773163607908792	21.668744256298737
13	24.960374148112425	23.711818603677788	31.710326378715774	19.61748086949401
14	19.872796920921175	28.67091890121289	28.611047924078502	22.845236253787434
15	17.629910011555644	24.726349599192016	36.320573596717104	21.323166792535236
16	19.848775738605863	24.457749106029862	31.14728442353712	24.546190731827156
17	19.550512724857374	24.49414483681064	29.503289264169315	26.45205317416267
18	20.442754064948183	24.81770288345177	30.124564388597218	24.61497866300283
19	22.18920320646388	26.371618609137148	27.565580557400615	23.873597626998354
20	25.29685267918073	25.87609073455684	28.259647143390083	20.56740944287235
21	23.1287589965697	28.408869639591277	27.516446320846565	20.945925042992457
22	20.407268227436923	25.155637243751304	26.660236754228727	27.776857774583043
23	21.288226890985687	27.386331583305278	27.036750589155893	24.28869093655314
24	23.566053701900767	23.885790196809914	27.52609118950347	25.022064911785847
25	20.22001219256981	28.229984622803745	25.45590202269274	26.094101161933704
26	20.703893433300273	25.951611875926954	28.467284787494428	24.877209903278345
27	23.168248364466848	27.274596689808284	26.618927599792546	22.938227345932322
28	19.0053046777613	27.226008389215945	28.574106257336013	25.19458067568674
29	22.395385021336995	24.441553005832418	27.579956871059025	25.583105101771565
30	25.845154363393174	25.746703911631165	26.672065366732483	21.736076358243178
31	24.44792225871905	27.65966352146893	23.978235352993092	23.914178866818922
32	25.945788559002033	26.13504635906208	23.750398078305416	24.168767003630474
33	26.660236754228727	24.39096294004713	23.13458231349463	25.81421799222951
34	22.520950292530685	24.17622812844053	28.679289919292465	24.623531659736315
35	25.389479814017818	22.461989208665823	26.1685304313804	25.98000054593596
36	24.729079279000572	25.481379034239282	26.309927845463726	23.479613841296416
37	23.63065612403665	26.06844217173326	27.806338316515472	22.49456338771462
38	23.71145464636998	27.227282239793272	23.444855918400773	25.616407195435976
39	24.101252923032128	22.902013593805446	27.0325650801161	25.964168403046322
40	26.730844471943442	21.679299018225162	29.38354730990055	22.20630919993085
41	22.02560439660428	23.19354339735949	27.36085457175873	27.4199976342775
42	23.83974959737223	22.982812116138778	27.567764301247465	25.609673985241532
43	24.570029935488567	22.235061827247666	26.698816228856355	26.496092008407413
44	22.932767986315206	23.314559202205583	27.390881049652876	26.361791761826336
45	23.489258709953322	21.246007843279983	27.05076294550649	28.213970501260206
46	25.68519512661165	23.251594587954834	26.93211286316115	24.13109742227237
47	21.10479240785056	22.3955669999909	32.112317225189486	24.387323366969056
48	22.267454027642557	22.029607926990163	29.207209994267675	26.495728051099604
49	22.852515399943588	20.166510468322066	31.10652120506265	25.874452926671704
50	21.983931284860287	20.467685140533014	29.837766030044676	27.710617544562023
51	21.363020117740188	20.744656651774747	27.21326988344267	30.67905334704239
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	399.0
1	686.5
2	974.0
3	1384.5
4	1795.0
5	1362.0
6	929.0
7	939.5
8	950.0
9	1028.0
10	1106.0
11	1172.5
12	1239.0
13	1314.0
14	1389.0
15	1383.0
16	1377.0
17	1273.0
18	1169.0
19	1331.5
20	1494.0
21	1529.5
22	1565.0
23	1574.0
24	1583.0
25	2153.5
26	3230.5
27	3737.0
28	4427.0
29	5117.0
30	5912.0
31	6707.0
32	7472.0
33	8237.0
34	9443.5
35	10650.0
36	11678.5
37	12707.0
38	13827.5
39	14948.0
40	16994.5
41	19041.0
42	21622.5
43	24204.0
44	26392.5
45	28581.0
46	31647.0
47	34713.0
48	49124.0
49	63535.0
50	62616.0
51	61697.0
52	59060.5
53	56424.0
54	47942.5
55	39461.0
56	34516.0
57	29571.0
58	27000.0
59	24429.0
60	23901.0
61	23373.0
62	21018.0
63	18663.0
64	16589.0
65	14515.0
66	12192.0
67	9869.0
68	8434.0
69	6999.0
70	6220.0
71	5441.0
72	4236.5
73	3032.0
74	2602.5
75	1855.0
76	1537.0
77	1102.5
78	668.0
79	558.0
80	448.0
81	327.0
82	206.0
83	158.0
84	110.0
85	60.5
86	11.0
87	11.0
88	11.0
89	6.0
90	1.0
91	2.5
92	4.0
93	3.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	549515.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.22976011891538
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.05936652032935	22.503967307508873
2	11.169138872993742	6.976190554768949
3	4.03698282220986	3.782220154253879
4	2.0233162312660733	2.527507221885895
5	1.218764184258412	1.9030856557957898
6	0.8711810789138121	1.6324065668769743
7	0.6729546983661011	1.4711349660609274
8	0.5534738719119765	1.3827885001518656
9	0.49826321487724673	1.4004576609026405
>10	6.711822331679981	45.200769369424194
>50	0.11804228319070365	2.4188524801133875
>100	0.05607008451558421	3.2033252497059017
>500	0.007082536991442217	1.6865427259801484
>1k	0.0035412684957211085	3.91075158657058
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGC	4889	0.8896936389361528	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCG	4411	0.8027078423700901	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTC	3617	0.6582167911703957	TruSeq Adapter, Index 22 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC	3062	0.5572186382537327	TruSeq Adapter, Index 22 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCT	2917	0.5308317334376678	Illumina Single End Adapter 2 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC	2321	0.4223724557109451	TruSeq Adapter, Index 22 (95% over 24bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGACGTGCTCGTA	960	0.1746995077477412	No Hit
TCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC	956	0.1739715931321256	TruSeq Adapter, Index 22 (95% over 24bp)
GAATGACTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCT	919	0.16723838293768142	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCC	846	0.15395394120269693	No Hit
GAACTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCT	788	0.14339917927627088	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTC	766	0.13939564889038517	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	756	0.1375758623513462	No Hit
ACTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC	732	0.13320837465765265	Illumina Single End Adapter 2 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGACGTGCT	710	0.12920484427176693	No Hit
CGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTG	630	0.11464655195945515	Illumina Single End Adapter 2 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTG	572	0.10409179003302911	TruSeq Adapter, Index 22 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5945242623040318	0.0
2	0.0	0.0	0.0	2.0350672866072808	0.0
3	0.0	0.0	0.0	2.8395949155164097	0.0
4	0.0	0.0	0.0	3.815000500441298	0.0
5	0.0	0.0	0.0	6.465519594551559	0.0
6	0.0	0.0	0.0	7.59979254433455	0.0
7	0.0	0.0	0.0	8.895662538784201	0.0
8	0.0	0.0	0.0	10.853388897482326	0.0
9	0.0	0.0	0.0	11.499777076148968	0.0
10	0.0	0.0	0.0	13.679699371263752	0.0
11	0.0	0.0	0.0	15.676187183243405	0.0
12	0.0	0.0	0.0	18.173116293458776	0.0
13	0.0	0.0	0.0	18.84698324886491	0.0
14	0.0	0.0	0.0	19.151433536846127	0.0
15	0.0	0.0	0.0	19.812925943786794	0.0
16	0.0	0.0	0.0	20.786511742172642	0.0
17	0.0	0.0	0.0	21.890212278099778	0.0
18	0.0	0.0	0.0	23.19063173889703	0.0
19	0.0	0.0	0.0	24.07613986879339	0.0
20	0.0	0.0	0.0	24.79713929556063	0.0
21	0.0	0.0	0.0	25.609673985241532	0.0
22	0.0	0.0	0.0	26.492816392637145	0.0
23	0.0	0.0	0.0	27.262040162688916	0.0
24	0.0	0.0	0.0	27.91206791443364	0.0
25	0.0	0.0	0.0	28.47583778422791	0.0
26	0.0	0.0	0.0	28.97828084765657	0.0
27	0.0	0.0	0.0	29.47526455146811	0.0
28	0.0	0.0	0.0	29.974613977780407	0.0
29	1.8197865390389708E-4	0.0	0.0	30.50617362583369	0.0
30	1.8197865390389708E-4	0.0	0.0	31.097240293713547	0.0
31	1.8197865390389708E-4	0.0	0.0	31.5993193998344	0.0
32	3.6395730780779416E-4	0.0	0.0	32.13033311192597	0.0
33	3.6395730780779416E-4	0.0	0.0	32.60202178284487	0.0
34	3.6395730780779416E-4	0.0	0.0	33.09918746531032	0.0
35	3.6395730780779416E-4	0.0	0.0	33.58670827911886	0.0
36	3.6395730780779416E-4	0.0	0.0	34.07095347715713	0.0
37	3.6395730780779416E-4	0.0	0.0	34.573760497893595	0.0
38	3.6395730780779416E-4	0.0	0.0	35.06310109824118	0.0
39	3.6395730780779416E-4	0.0	0.0	35.563178439169086	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTAC	35	1.2103737E-7	45.000004	8
TACGGGT	70	0.0	45.000004	4
GTATACG	35	1.2103737E-7	45.000004	1
CGGGTTA	25	3.8881393E-5	45.0	6
AATCCGT	20	7.030069E-4	45.0	42
TCGTTCA	25	3.8881393E-5	45.0	16
ACACGAC	25	3.8881393E-5	45.0	26
ATTAGCG	20	7.030069E-4	45.0	1
GGCGTAT	20	7.030069E-4	45.0	6
ATTACGG	20	7.030069E-4	45.0	2
AGGCGTA	20	7.030069E-4	45.0	5
CACGACC	25	3.8881393E-5	45.0	27
AAACGCG	20	7.030069E-4	45.0	1
TCGGATC	20	7.030069E-4	45.0	40
TGATTAC	20	7.030069E-4	45.0	11
AACGAGC	40	6.8030204E-9	45.0	15
ATGCGTC	20	7.030069E-4	45.0	10
AACCTAG	25	3.8881393E-5	45.0	21
CACGTAG	20	7.030069E-4	45.0	1
CACAACG	40	6.8030204E-9	45.0	12
>>END_MODULE