SRR3549961_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549961_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1389280
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCT	4200	0.3023148681331337	TruSeq Adapter, Index 22 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3953	0.2845358746976851	No Hit
GCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC	3906	0.28115282736381436	TruSeq Adapter, Index 23 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC	3855	0.277481861107912	TruSeq Adapter, Index 23 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCC	1958	0.14093631233444662	No Hit
TCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC	1624	0.11689508234481169	TruSeq Adapter, Index 23 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTG	1473	0.10602614303812047	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATACG	20	7.033797E-4	45.0	1
TATCGAG	40	3.4589175E-7	39.375	1
CGTTTTT	2320	0.0	38.30819	1
TATACGG	95	0.0	37.894737	2
AGTACGG	155	0.0	37.74194	2
TAAGTCG	30	1.1400446E-4	37.499996	1
TACGGGT	120	0.0	37.499996	4
CGCTCGA	30	1.1400446E-4	37.499996	44
TACCGGT	60	1.5643309E-10	37.499996	40
GTAGGGA	1055	0.0	36.255928	4
TAGGGAT	825	0.0	36.0	5
TCGTTTA	25	0.00210726	35.999996	38
TCGCAAT	25	0.00210726	35.999996	30
ATCGTCC	25	0.00210726	35.999996	19
AATGGGC	500	0.0	35.100002	4
TACGCGC	45	8.7016633E-7	35.0	34
TATCGCG	45	8.7016633E-7	35.0	1
TTAAGCG	65	3.6743586E-10	34.615383	1
TTAGCGG	290	0.0	34.137928	2
AATGCGG	205	0.0	34.02439	2