##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549961_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1389280
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.33450060462974	33.0	31.0	34.0	30.0	34.0
2	32.508412271104454	34.0	31.0	34.0	31.0	34.0
3	32.49920390418058	34.0	31.0	34.0	31.0	34.0
4	36.02090003455027	37.0	35.0	37.0	35.0	37.0
5	35.90587642519866	37.0	35.0	37.0	35.0	37.0
6	35.863473165956464	37.0	35.0	37.0	35.0	37.0
7	36.073658297823336	37.0	35.0	37.0	35.0	37.0
8	36.05152812967868	37.0	35.0	37.0	35.0	37.0
9	37.60165697339629	39.0	38.0	39.0	35.0	39.0
10	37.374872595877	39.0	37.0	39.0	34.0	39.0
11	37.27074167914315	39.0	37.0	39.0	34.0	39.0
12	37.183102758263274	39.0	37.0	39.0	34.0	39.0
13	37.156730104802485	39.0	37.0	39.0	34.0	39.0
14	38.32890777956927	40.0	38.0	41.0	34.0	41.0
15	38.41784161580099	40.0	38.0	41.0	34.0	41.0
16	38.42908700909824	40.0	38.0	41.0	34.0	41.0
17	38.443318121616954	40.0	38.0	41.0	34.0	41.0
18	38.39781109639525	40.0	38.0	41.0	34.0	41.0
19	38.34555885062766	40.0	37.0	41.0	34.0	41.0
20	38.30186643441207	40.0	37.0	41.0	34.0	41.0
21	38.28750503858114	40.0	37.0	41.0	34.0	41.0
22	38.17893585166417	40.0	37.0	41.0	34.0	41.0
23	37.930750460670275	40.0	36.0	41.0	33.0	41.0
24	37.733606616376825	40.0	36.0	41.0	33.0	41.0
25	37.73917424853161	40.0	36.0	41.0	33.0	41.0
26	37.82031483934124	40.0	36.0	41.0	33.0	41.0
27	37.89465406541518	40.0	36.0	41.0	34.0	41.0
28	37.84511257629851	40.0	36.0	41.0	33.0	41.0
29	37.83075765864333	40.0	36.0	41.0	33.0	41.0
30	37.79540049522055	40.0	36.0	41.0	33.0	41.0
31	37.63186470689854	40.0	36.0	41.0	33.0	41.0
32	37.531035500403085	40.0	36.0	41.0	33.0	41.0
33	37.48243406656685	40.0	36.0	41.0	33.0	41.0
34	37.39707402395486	40.0	36.0	41.0	33.0	41.0
35	37.33786709662559	40.0	36.0	41.0	33.0	41.0
36	37.285658038696305	40.0	35.0	41.0	32.0	41.0
37	37.21239562939076	39.0	35.0	41.0	32.0	41.0
38	37.10797391454567	39.0	35.0	41.0	32.0	41.0
39	37.07530015547622	39.0	35.0	41.0	32.0	41.0
40	37.03396939421859	39.0	35.0	41.0	32.0	41.0
41	36.9397400092134	39.0	35.0	41.0	32.0	41.0
42	36.89598281124035	39.0	35.0	41.0	32.0	41.0
43	36.810689709777726	39.0	35.0	41.0	31.0	41.0
44	36.718002850397326	39.0	35.0	41.0	31.0	41.0
45	36.6511761487965	39.0	35.0	41.0	31.0	41.0
46	36.53564004376368	39.0	35.0	41.0	31.0	41.0
47	36.42207042496833	39.0	35.0	41.0	31.0	41.0
48	36.34688903604745	39.0	35.0	40.0	31.0	41.0
49	36.32801523091098	39.0	35.0	40.0	31.0	41.0
50	36.24702867672463	38.0	35.0	40.0	31.0	41.0
51	35.2837016296211	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	11.0
10	13.0
11	14.0
12	12.0
13	27.0
14	26.0
15	48.0
16	87.0
17	136.0
18	313.0
19	519.0
20	926.0
21	1505.0
22	2237.0
23	3336.0
24	4908.0
25	6685.0
26	9005.0
27	11068.0
28	13055.0
29	15406.0
30	18862.0
31	24359.0
32	31979.0
33	44678.0
34	81264.0
35	105215.0
36	106641.0
37	164053.0
38	294567.0
39	448023.0
40	298.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.18471438442934	21.402669008407234	25.363857537717376	12.048759069446044
2	32.51965046642865	24.510969710929402	24.85683231601981	18.112547506622136
3	31.789775999078664	24.003152712196247	26.893138892087986	17.313932396637107
4	29.18475757226765	25.371343429690203	26.40885926523091	19.03503973281124
5	25.882615455487734	29.49376655533802	24.78693999769665	19.8366779914776
6	26.699513417021763	33.88489001497179	23.85163537947714	15.56396118852931
7	79.98898710123228	6.261804675803294	8.80967119659104	4.939537026373373
8	80.53531325578717	4.932986870897156	8.910082920649545	5.6216169526661295
9	74.14696821375101	7.241448808015663	11.41022688011056	7.201356098122769
10	39.55386963031211	25.227311988943914	18.526862835425543	16.691955545318436
11	28.881219048715884	25.76068179200737	24.96098698606472	20.397112173212022
12	25.978708395715767	22.793173442358633	30.256895658182653	20.971222503742943
13	23.288250028791893	23.264712656915812	32.51216457445583	20.934872739836464
14	20.33765691581251	26.60190890245307	31.18104341817344	21.879390763560984
15	20.05218530461822	25.24422722561327	33.8129102844639	20.890677185304618
16	22.2217263618565	25.17764597489347	30.787026373373262	21.81360128987677
17	21.248848324311872	25.447354025106527	29.11558505124957	24.18821259933203
18	22.250014395946103	25.034622250374294	30.253512610848787	22.46185074283082
19	22.772803178624898	26.18118737763446	28.084259472532537	22.96174997120811
20	24.308706668202234	26.53777496257054	28.037544627432915	21.11597374179431
21	24.059656800644937	26.309023379016466	28.655994471956696	20.975325348381897
22	22.05048658297823	25.200175630542436	27.649789819186914	25.099547967292413
23	22.13894967177243	25.793144650466427	28.379088448692848	23.68881722906829
24	22.861626166071634	24.450722676494298	28.81521363584015	23.87243752159392
25	21.936110791201198	26.013906483934125	27.10727859034896	24.94270413451572
26	21.349044109178855	26.312118507428305	27.767476678567316	24.57136070482552
27	23.11715420937464	25.396320396176435	27.756391800069103	23.730133594379822
28	20.541719451802372	25.999870436485082	28.913753886905447	24.544656224807092
29	21.857868824139125	24.54580790049522	28.170203270758954	25.4261200046067
30	23.71861683749856	23.94736842105263	27.736597374179432	24.59741736726938
31	22.372595877001036	26.2171772428884	27.529799608430267	23.880427271680293
32	22.549522054589428	25.876713117586085	27.072944258896698	24.50082056892779
33	22.654252562478405	25.261862259587698	27.0820856846712	25.0017994932627
34	20.23666935390994	24.384501324427042	30.07629851433836	25.302530807324658
35	21.14195842450766	24.585540711735575	28.578328342738686	25.694172521018082
36	21.897817574571	26.086534032016583	26.72693769434527	25.288710699067142
37	21.19234423586318	26.40576413681907	30.044267534262353	22.357624093055396
38	20.655159507082804	26.653806288149255	28.505628814925714	24.18540538984222
39	21.97253253483819	23.900509616492	28.634112633882296	25.49284521478752
40	21.499121847287803	24.051307151906023	30.13460209604975	24.31496890475642
41	19.707978233329495	26.0547189911321	27.845214787515836	26.392087988022574
42	21.495666820223423	25.30137913163653	27.74984164459288	25.453112403547163
43	22.515187723137164	23.10866060117471	29.418835655879306	24.95731601980882
44	21.53979039502476	23.154799608430267	28.72574283081884	26.579667165726136
45	22.17803466543821	22.573851203501093	28.88028331221928	26.367830818841416
46	22.37216399861799	23.973569042957504	28.619644708050213	25.034622250374294
47	20.41633076125763	23.859193251180468	30.543878843717607	25.18059714384429
48	20.114159852585512	23.03804848554647	30.560074283081885	26.287717378786134
49	21.30117758839111	22.448750431878384	31.064004376367617	25.186067603362893
50	20.06895658182656	22.23475469307843	31.08127951168951	26.615009213405504
51	19.99964010134746	22.177530807324658	28.856889899804216	28.965939191523667
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	640.0
1	1488.0
2	2336.0
3	4787.5
4	7239.0
5	4961.5
6	2684.0
7	2607.5
8	2531.0
9	2536.5
10	2542.0
11	2708.5
12	2875.0
13	2737.5
14	2600.0
15	2680.0
16	2760.0
17	2789.5
18	2819.0
19	2892.0
20	2965.0
21	3496.5
22	4028.0
23	4458.5
24	4889.0
25	6273.5
26	10077.5
27	12497.0
28	15040.0
29	17583.0
30	19409.5
31	21236.0
32	24423.0
33	27610.0
34	30155.5
35	32701.0
36	35280.5
37	37860.0
38	41983.0
39	46106.0
40	51906.5
41	57707.0
42	64164.0
43	70621.0
44	79658.0
45	88695.0
46	102938.0
47	117181.0
48	132456.5
49	147732.0
50	143841.0
51	139950.0
52	121970.5
53	103991.0
54	91086.5
55	78182.0
56	72411.5
57	66641.0
58	64316.0
59	61991.0
60	59519.5
61	57048.0
62	51427.0
63	45806.0
64	40786.0
65	35766.0
66	29962.5
67	24159.0
68	20525.0
69	16891.0
70	14669.5
71	12448.0
72	10471.5
73	8495.0
74	6768.0
75	4035.0
76	3029.0
77	2507.5
78	1986.0
79	1493.0
80	1000.0
81	701.0
82	402.0
83	307.0
84	212.0
85	141.0
86	70.0
87	53.0
88	36.0
89	27.5
90	19.0
91	16.0
92	13.0
93	9.0
94	5.0
95	4.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1389280.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.93104574353989
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.09894141428738	20.56966726374011
2	11.083298326813784	6.413028217646974
3	4.062985535692081	3.5263926116534563
4	2.032194834600875	2.351740868784936
5	1.2463316974948981	1.802883967592431
6	0.8462646126796967	1.4689992132345222
7	0.6469655106777655	1.3102172148737756
8	0.5590994065482496	1.2940264404826733
9	0.4553193691716436	1.1855578947682095
>10	7.725056756648006	50.79473744841404
>50	0.18033531144182963	3.2683082211204173
>100	0.05540075419521173	3.0891817878840255
>500	0.004784610491091066	0.9855773490773687
>1k	0.0030218592575312	1.9396815007270745
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCT	4200	0.3023148681331337	TruSeq Adapter, Index 22 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3953	0.2845358746976851	No Hit
GCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC	3906	0.28115282736381436	TruSeq Adapter, Index 23 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC	3855	0.277481861107912	TruSeq Adapter, Index 23 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCC	1958	0.14093631233444662	No Hit
TCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC	1624	0.11689508234481169	TruSeq Adapter, Index 23 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTG	1473	0.10602614303812047	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4395946101577797E-4	0.0	0.0	0.3208136588736612	0.0
2	1.4395946101577797E-4	0.0	0.0	1.1316653230450304	0.0
3	1.4395946101577797E-4	0.0	0.0	1.7192358631809284	0.0
4	1.4395946101577797E-4	0.0	0.0	2.4025394448923185	0.0
5	1.4395946101577797E-4	0.0	0.0	3.949887711620408	0.0
6	1.4395946101577797E-4	0.0	0.0	5.054272716802949	0.0
7	1.4395946101577797E-4	0.0	0.0	6.128930093285731	0.0
8	1.4395946101577797E-4	0.0	0.0	7.7706437867096625	0.0
9	1.4395946101577797E-4	0.0	0.0	8.38175169872164	0.0
10	1.4395946101577797E-4	0.0	0.0	9.571792583208568	0.0
11	1.4395946101577797E-4	0.0	0.0	11.361784521478752	0.0
12	1.4395946101577797E-4	0.0	0.0	12.610560866060117	0.0
13	1.4395946101577797E-4	0.0	0.0	13.151704480018427	0.0
14	1.4395946101577797E-4	0.0	0.0	13.372466313486122	0.0
15	1.4395946101577797E-4	0.0	0.0	13.78440631118277	0.0
16	2.1593919152366694E-4	0.0	0.0	14.565458366923874	0.0
17	2.1593919152366694E-4	0.0	0.0	15.462181849591156	0.0
18	2.8791892203155594E-4	0.0	0.0	16.446144765634	0.0
19	2.8791892203155594E-4	0.0	0.0	17.098928941610044	0.0
20	2.8791892203155594E-4	0.0	0.0	17.73494184037775	0.0
21	2.8791892203155594E-4	0.0	0.0	18.516641713693424	0.0
22	2.8791892203155594E-4	0.0	0.0	19.299205343775192	0.0
23	2.8791892203155594E-4	0.0	0.0	20.058735460094436	0.0
24	2.8791892203155594E-4	0.0	0.0	20.69971496026719	0.0
25	2.8791892203155594E-4	0.0	0.0	21.267563054243926	0.0
26	2.8791892203155594E-4	0.0	0.0	21.775236093516067	0.0
27	2.8791892203155594E-4	0.0	0.0	22.275207301623862	0.0
28	2.8791892203155594E-4	0.0	0.0	22.793173442358633	0.0
29	3.598986525394449E-4	0.0	0.0	23.307324657376483	0.0
30	3.598986525394449E-4	0.0	0.0	23.898422204307266	0.0
31	3.598986525394449E-4	0.0	0.0	24.44971496026719	0.0
32	3.598986525394449E-4	0.0	0.0	24.987187607969595	0.0
33	3.598986525394449E-4	0.0	0.0	25.519837613727976	0.0
34	4.318783830473339E-4	0.0	0.0	26.018657146147646	0.0
35	4.318783830473339E-4	0.0	0.0	26.547420246458596	0.0
36	4.318783830473339E-4	0.0	0.0	27.075319590003456	0.0
37	4.318783830473339E-4	0.0	0.0	27.604154670044917	0.0
38	4.318783830473339E-4	0.0	0.0	28.1222647702407	0.0
39	4.318783830473339E-4	0.0	0.0	28.649660255672003	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATACG	20	7.033797E-4	45.0	1
TATCGAG	40	3.4589175E-7	39.375	1
CGTTTTT	2320	0.0	38.30819	1
TATACGG	95	0.0	37.894737	2
AGTACGG	155	0.0	37.74194	2
TAAGTCG	30	1.1400446E-4	37.499996	1
TACGGGT	120	0.0	37.499996	4
CGCTCGA	30	1.1400446E-4	37.499996	44
TACCGGT	60	1.5643309E-10	37.499996	40
GTAGGGA	1055	0.0	36.255928	4
TAGGGAT	825	0.0	36.0	5
TCGTTTA	25	0.00210726	35.999996	38
TCGCAAT	25	0.00210726	35.999996	30
ATCGTCC	25	0.00210726	35.999996	19
AATGGGC	500	0.0	35.100002	4
TACGCGC	45	8.7016633E-7	35.0	34
TATCGCG	45	8.7016633E-7	35.0	1
TTAAGCG	65	3.6743586E-10	34.615383	1
TTAGCGG	290	0.0	34.137928	2
AATGCGG	205	0.0	34.02439	2
>>END_MODULE