FastQCFastQC Report
Sat 18 Jun 2016
SRR3549959_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549959_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences951336
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGCT37690.3961796883540621No Hit
CCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC34370.36128139794983055No Hit
GCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC33420.35129544135825824No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16560.17407099069098614No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCC13360.14043408427726903No Hit
TCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC12830.1348629716524971No Hit
ACTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC10930.11489105846935257No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATAG207.0326723E-445.0000041
ACGTATG207.0326723E-445.0000041
ATAACGC406.8121153E-945.00000411
TACCGAC207.0326723E-445.00000414
CGTACCA253.890298E-545.022
CGTAAGG1350.041.6666642
GCGTTAG1050.040.7142871
TAACGCC451.927765E-840.00000412
CGTTCAT800.039.37500417
GTATGCG403.4573895E-739.3750041
GTTGACG700.038.5714261
TTTAGCG356.2471536E-638.5714261
CTATCGG356.2471536E-638.5714262
TAACGCG356.2471536E-638.5714261
GTATACG356.2471536E-638.5714261
TTACGGT356.2471536E-638.57142634
TACGTGT356.2471536E-638.57142612
CGTTAGG1850.037.70272
CGTTCGG1500.037.545
TCGCATC301.1397741E-437.49999624