SRR3549958_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549958_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1197751
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGCT	3960	0.3306196363017021	Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGC	3100	0.2588184021553729	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3012	0.25147129912644617	No Hit
CCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGC	2822	0.2356082357685362	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCC	1847	0.15420567380031408	No Hit
TCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGC	1237	0.10327689144070847	Illumina Single End Adapter 1 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCATC	20	7.033407E-4	45.0	21
CCCGTAT	20	7.033407E-4	45.0	29
GTATACG	35	1.2119926E-7	45.0	1
AGAATCG	20	7.033407E-4	45.0	30
ATCGATA	20	7.033407E-4	45.0	10
CGTAAGG	135	0.0	41.666664	2
ACCGGTA	40	3.45839E-7	39.375	41
TTAATCG	80	0.0	39.375	20
GATCGAT	40	3.45839E-7	39.375	9
TAACGCC	75	0.0	39.0	12
TCGATAG	75	0.0	39.0	1
AATACGG	105	0.0	38.57143	2
TCTTACG	35	6.2485105E-6	38.571426	1
CGGAATA	30	1.1399504E-4	37.500004	42
CGTTGAT	85	0.0	37.058823	25
GTACGAG	85	0.0	37.058823	1
GCATCGT	55	2.750312E-9	36.81818	35
CGTTTTT	1945	0.0	36.786633	1
ATAACGC	80	0.0	36.5625	11
CATTACG	25	0.002107144	36.0	35