##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549958_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1197751
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.362597067337035	33.0	31.0	34.0	31.0	34.0
2	32.53456436270978	34.0	31.0	34.0	31.0	34.0
3	32.528660798446424	34.0	31.0	34.0	31.0	34.0
4	36.04730866432172	37.0	35.0	37.0	35.0	37.0
5	35.9326955268666	37.0	35.0	37.0	35.0	37.0
6	35.89343527995385	37.0	35.0	37.0	35.0	37.0
7	36.084937520402825	37.0	35.0	37.0	35.0	37.0
8	36.05587054404463	37.0	35.0	37.0	35.0	37.0
9	37.61475799227051	39.0	38.0	39.0	35.0	39.0
10	37.39489843882409	39.0	37.0	39.0	34.0	39.0
11	37.280252740344196	39.0	37.0	39.0	34.0	39.0
12	37.172479922788625	39.0	37.0	39.0	34.0	39.0
13	37.13716289946742	39.0	37.0	39.0	33.0	39.0
14	38.328496490505955	40.0	38.0	41.0	34.0	41.0
15	38.41409274548717	40.0	38.0	41.0	34.0	41.0
16	38.44106412768597	40.0	38.0	41.0	34.0	41.0
17	38.45229517654337	40.0	38.0	41.0	34.0	41.0
18	38.399369317996815	40.0	37.0	41.0	34.0	41.0
19	38.34405147647549	40.0	37.0	41.0	34.0	41.0
20	38.28913021153812	40.0	37.0	41.0	34.0	41.0
21	38.25335816876797	40.0	37.0	41.0	34.0	41.0
22	38.15901218199776	40.0	37.0	41.0	34.0	41.0
23	37.91372580778476	40.0	36.0	41.0	33.0	41.0
24	37.71677669231752	40.0	36.0	41.0	33.0	41.0
25	37.712089574544294	40.0	36.0	41.0	33.0	41.0
26	37.78069982826147	40.0	36.0	41.0	33.0	41.0
27	37.85099741098108	40.0	36.0	41.0	33.0	41.0
28	37.816300716926975	40.0	36.0	41.0	33.0	41.0
29	37.808395067088234	40.0	36.0	41.0	33.0	41.0
30	37.740426015089945	40.0	36.0	41.0	33.0	41.0
31	37.60273879963365	40.0	36.0	41.0	33.0	41.0
32	37.49983260293667	40.0	36.0	41.0	33.0	41.0
33	37.40247931331304	40.0	35.0	41.0	33.0	41.0
34	37.27450864161249	40.0	35.0	41.0	33.0	41.0
35	37.205172861471205	40.0	35.0	41.0	32.0	41.0
36	37.10022283429527	39.0	35.0	41.0	32.0	41.0
37	37.00176330472694	39.0	35.0	41.0	32.0	41.0
38	36.892522736361734	39.0	35.0	41.0	31.0	41.0
39	36.81012497589232	39.0	35.0	41.0	31.0	41.0
40	36.70207246748281	39.0	35.0	41.0	31.0	41.0
41	36.65111863818106	39.0	35.0	41.0	31.0	41.0
42	36.599415070411126	39.0	35.0	41.0	31.0	41.0
43	36.492821128932476	39.0	35.0	41.0	31.0	41.0
44	36.3885715812385	39.0	35.0	41.0	31.0	41.0
45	36.33280706924895	39.0	35.0	40.0	31.0	41.0
46	36.24345961723263	38.0	35.0	40.0	31.0	41.0
47	36.13957241530168	38.0	35.0	40.0	30.0	41.0
48	36.05743180343828	38.0	35.0	40.0	30.0	41.0
49	36.013633050609016	38.0	35.0	40.0	30.0	41.0
50	35.89495646424006	38.0	35.0	40.0	30.0	41.0
51	34.94728620556359	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	7.0
10	13.0
11	7.0
12	14.0
13	13.0
14	19.0
15	49.0
16	81.0
17	162.0
18	274.0
19	556.0
20	918.0
21	1496.0
22	2216.0
23	3273.0
24	4516.0
25	6063.0
26	8309.0
27	10038.0
28	11510.0
29	13638.0
30	16850.0
31	21453.0
32	28057.0
33	39075.0
34	73396.0
35	96441.0
36	95159.0
37	145844.0
38	251620.0
39	366448.0
40	235.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.15596647383305	21.709019654335503	25.082174842684328	12.052839029147126
2	32.102331786823804	24.708390976087685	25.379565535741573	17.80971170134694
3	31.769374435921993	23.744751621998226	27.290062792683955	17.195811149395826
4	28.733434578639468	25.515069492740977	26.62615184625185	19.125344082367704
5	25.26910852088623	30.26614045824216	24.889062918753563	19.575688102118054
6	27.184448186643134	34.07511243989777	23.778648483699868	14.961790889759225
7	80.19646821417807	6.2464569013092035	8.481312059017275	5.075762825495449
8	80.90337641129082	5.0797703362385	8.729026316822111	5.287826935648561
9	74.71886894688463	7.092041668092951	11.168181032618634	7.020908352403797
10	42.03636649019705	23.16190927830576	18.985749124818096	15.815975106679101
11	30.34645765271747	24.35172251995615	24.64936368243483	20.652456144891552
12	26.51352409641069	21.70259093918519	30.0794572494617	21.70442771494242
13	24.063682685299366	22.842644255776033	31.676533770374643	21.417139288549958
14	20.590256238567115	26.413169348220123	30.388870474748092	22.607703938464674
15	20.036134388533174	25.111396275185744	33.38356636730005	21.468902968981034
16	22.582573506513455	24.255124813087196	30.584821052121853	22.577480628277495
17	22.675414172060805	23.824985326666397	28.281504252553326	25.218096248719473
18	22.9551050260029	24.174974598226175	29.526921705763552	23.342998670007372
19	24.64961415185627	24.846566606915797	27.306510284691893	23.19730895653604
20	25.453954953909452	25.533186780891853	27.042098065457676	21.970760199741015
21	24.385953340886378	26.02961717418729	27.849027051532417	21.73540243339392
22	22.771844899315465	24.122542999337927	27.068313864901803	26.037298236444805
23	23.50956083526543	25.061051921476167	26.92270764123762	24.506679602020785
24	23.645565731107716	24.187665048912503	28.066684978764368	24.10008424121541
25	23.180193546070928	25.10889158097134	26.413920756484444	25.296994116473293
26	22.271240015662688	26.094989693183308	26.71974392006352	24.914026371090486
27	23.13719629539028	25.407200661907194	27.866392931418964	23.589210111283563
28	21.451453599287333	25.607325729638298	28.45716680679039	24.48405386428398
29	23.00569984913392	24.626654454890875	27.312354571192177	25.05529112478303
30	23.626613544885373	24.911939125911815	27.645311922093992	23.816135407108824
31	24.007160085860917	26.721664185627898	25.38156929111309	23.88960643739809
32	25.273533480665012	25.66877422769841	25.069484391998003	23.98820789963857
33	24.922458841612322	26.097494387397713	25.649237612826038	23.330809158163927
34	23.05370648824338	25.532351882820386	27.463220652706617	23.95072097622962
35	24.76691733089766	24.356731908384965	26.130305881606443	24.746044879110933
36	24.30630406486824	26.827195301861572	26.427195635820798	22.439304997449387
37	24.469944086876154	26.683759813183205	27.849110541339563	20.99718555860108
38	25.00586515895207	26.394885080454955	25.80720032794796	22.792049432645015
39	25.49252724481132	25.693904659649625	25.727300582508384	23.086267513030673
40	24.432540653274344	24.898413777154015	27.68505307029591	22.983992499275725
41	22.843646133461796	25.323543875146004	26.505843034153177	25.326966957239026
42	24.780693149076896	23.94287293435781	27.337318023529093	23.9391158930362
43	24.933729965577154	22.844814990761854	28.367665733528924	23.853789310132072
44	23.665018856172946	23.85788031068227	27.326631328214297	25.150469504930488
45	23.426822436382857	23.55840237244636	27.371882803687914	25.642892387482874
46	23.984701327738403	25.263514703807388	27.1840307376074	23.56775323084681
47	22.89891638579304	24.41325450782341	28.992920899252017	23.694908207131533
48	22.89816497752872	23.399103820410087	29.02982339401094	24.672907808050255
49	23.77180231951382	23.18929393504994	29.302083655116967	23.736820090319274
50	22.35581519030249	23.201232977471946	29.096782219342753	25.346169612882814
51	22.469194348407974	23.408120719581948	27.1356066494622	26.987078282547877
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	688.0
1	1400.5
2	2113.0
3	3912.0
4	5711.0
5	4053.5
6	2396.0
7	2354.0
8	2312.0
9	2414.5
10	2517.0
11	2555.5
12	2594.0
13	2698.0
14	2802.0
15	2871.0
16	2940.0
17	2924.5
18	2909.0
19	2954.5
20	3000.0
21	3045.0
22	3090.0
23	3496.0
24	3902.0
25	5142.0
26	7723.0
27	9064.0
28	10555.0
29	12046.0
30	14036.5
31	16027.0
32	18499.5
33	20972.0
34	23472.5
35	25973.0
36	27963.5
37	29954.0
38	32546.5
39	35139.0
40	39917.5
41	44696.0
42	50904.0
43	57112.0
44	64423.0
45	71734.0
46	83631.5
47	95529.0
48	107164.5
49	118800.0
50	116747.0
51	114694.0
52	100604.5
53	86515.0
54	78474.5
55	70434.0
56	65756.0
57	61078.0
58	58915.0
59	56752.0
60	55097.5
61	53443.0
62	51015.5
63	48588.0
64	42992.0
65	37396.0
66	32034.0
67	26672.0
68	22980.5
69	19289.0
70	16997.5
71	14706.0
72	12576.5
73	10447.0
74	8899.5
75	5866.0
76	4380.0
77	3579.5
78	2779.0
79	2156.0
80	1533.0
81	1098.5
82	664.0
83	519.0
84	374.0
85	245.5
86	117.0
87	108.0
88	99.0
89	60.5
90	22.0
91	16.5
92	11.0
93	7.0
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1197751.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.48429318155888
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.00901285955726	20.51125834006272
2	10.668268254505811	6.077561614017219
3	3.746771020569878	3.2017237270224292
4	1.9032746016803332	2.1685372703710923
5	1.1811880316119567	1.6822653097491702
6	0.8221857649778677	1.4051628227600306
7	0.6308282836707013	1.2578088445507094
8	0.5299351036297494	1.2075861487191653
9	0.45292595961267085	1.1611148240831592
>10	7.754682412204953	51.80880133432368
>50	0.23830934916706553	4.1762211567931375
>100	0.05549589145983306	2.9633778674162623
>500	0.004748311568005502	0.849891632713042
>1k	0.002374155784002751	1.528689107418207
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGCT	3960	0.3306196363017021	Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGC	3100	0.2588184021553729	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3012	0.25147129912644617	No Hit
CCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGC	2822	0.2356082357685362	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCC	1847	0.15420567380031408	No Hit
TCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGC	1237	0.10327689144070847	Illumina Single End Adapter 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.504694214406834E-4	0.0	0.0	0.3559170478672111	0.0
2	3.339592285875779E-4	0.0	0.0	1.0942174124671988	0.0
3	3.339592285875779E-4	0.0	0.0	1.609266032756391	0.0
4	3.339592285875779E-4	0.0	0.0	2.2112275422855	0.0
5	3.339592285875779E-4	0.0	0.0	3.601249341474146	0.0
6	3.339592285875779E-4	0.0	0.0	4.535416793640748	0.0
7	3.339592285875779E-4	0.0	0.0	5.437858119091531	0.0
8	3.339592285875779E-4	0.0	0.0	6.891081702290376	0.0
9	3.339592285875779E-4	0.0	0.0	7.4386913473668566	0.0
10	3.339592285875779E-4	0.0	0.0	8.541174250741598	0.0
11	3.339592285875779E-4	0.0	0.0	10.13591305705443	0.0
12	3.339592285875779E-4	0.0	0.0	11.351357669498919	0.0
13	3.339592285875779E-4	0.0	0.0	11.831591040207856	0.0
14	4.1744903573447237E-4	0.0	0.0	12.045575415925347	0.0
15	4.1744903573447237E-4	0.0	0.0	12.418106935414789	0.0
16	4.1744903573447237E-4	0.0	0.0	13.14555362508568	0.0
17	4.1744903573447237E-4	0.0	0.0	13.971184327961321	0.0
18	4.1744903573447237E-4	0.0	0.0	14.929313354779081	4.1744903573447237E-4
19	4.1744903573447237E-4	0.0	0.0	15.507981208114208	4.1744903573447237E-4
20	4.1744903573447237E-4	0.0	0.0	16.094330123706847	4.1744903573447237E-4
21	4.1744903573447237E-4	0.0	0.0	16.761580662424826	4.1744903573447237E-4
22	4.1744903573447237E-4	0.0	0.0	17.44419332565784	4.1744903573447237E-4
23	4.1744903573447237E-4	0.0	0.0	18.123549886412118	4.1744903573447237E-4
24	4.1744903573447237E-4	0.0	0.0	18.676085430110266	4.1744903573447237E-4
25	4.1744903573447237E-4	0.0	0.0	19.17435259916293	4.1744903573447237E-4
26	4.1744903573447237E-4	0.0	0.0	19.625615006791897	4.1744903573447237E-4
27	5.009388428813668E-4	0.0	0.0	20.075458087699364	4.1744903573447237E-4
28	5.009388428813668E-4	0.0	0.0	20.549095763643695	4.1744903573447237E-4
29	5.009388428813668E-4	0.0	0.0	21.03149986933845	4.1744903573447237E-4
30	5.009388428813668E-4	0.0	0.0	21.566168594307165	4.1744903573447237E-4
31	5.009388428813668E-4	0.0	0.0	22.067023947381383	4.1744903573447237E-4
32	5.009388428813668E-4	0.0	0.0	22.55351905362634	4.1744903573447237E-4
33	5.009388428813668E-4	0.0	0.0	23.03684154719971	4.1744903573447237E-4
34	5.009388428813668E-4	0.0	0.0	23.507724059508195	4.1744903573447237E-4
35	5.009388428813668E-4	0.0	0.0	23.99012816520295	4.1744903573447237E-4
36	5.844286500282613E-4	0.0	0.0	24.458756452718468	4.1744903573447237E-4
37	5.844286500282613E-4	0.0	0.0	24.934648353455767	4.1744903573447237E-4
38	5.844286500282613E-4	0.0	0.0	25.387163108191935	4.1744903573447237E-4
39	5.844286500282613E-4	0.0	0.0	25.856542803971777	4.1744903573447237E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCATC	20	7.033407E-4	45.0	21
CCCGTAT	20	7.033407E-4	45.0	29
GTATACG	35	1.2119926E-7	45.0	1
AGAATCG	20	7.033407E-4	45.0	30
ATCGATA	20	7.033407E-4	45.0	10
CGTAAGG	135	0.0	41.666664	2
ACCGGTA	40	3.45839E-7	39.375	41
TTAATCG	80	0.0	39.375	20
GATCGAT	40	3.45839E-7	39.375	9
TAACGCC	75	0.0	39.0	12
TCGATAG	75	0.0	39.0	1
AATACGG	105	0.0	38.57143	2
TCTTACG	35	6.2485105E-6	38.571426	1
CGGAATA	30	1.1399504E-4	37.500004	42
CGTTGAT	85	0.0	37.058823	25
GTACGAG	85	0.0	37.058823	1
GCATCGT	55	2.750312E-9	36.81818	35
CGTTTTT	1945	0.0	36.786633	1
ATAACGC	80	0.0	36.5625	11
CATTACG	25	0.002107144	36.0	35
>>END_MODULE