FastQCFastQC Report
Sat 18 Jun 2016
SRR3549957_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549957_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1083736
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGCT39570.3651258240014173No Hit
GCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC29610.2732215225848362No Hit
CCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC23830.2198875002768202No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20190.18629998449806964No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCC13110.1209704208404999No Hit
TCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC11500.10611440424605255No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTACG302.1653868E-645.0000041
CGGTCAT207.0331065E-445.00000419
GCGTATG700.041.7857171
ATTAGCG603.6379788E-1241.2500041
TAACACG603.6379788E-1241.2500041
TACGGGA3100.039.9193534
AATAGGC403.4579716E-739.37500414
TACGGGT750.039.04
AACACGT5400.038.75000441
TATAGCG356.2479576E-638.571431
CGAGGGT1700.038.382354
AATGCGA659.094947E-1238.0769225
ACACGAC5950.037.81512526
ACGACCA5900.037.7542428
ATAGACG601.5643309E-1037.5000041
CGCGAGT301.1398788E-437.50000416
TCGTCCA301.1398788E-437.50000414
TCAACGG601.5643309E-1037.5000042
TAGTACG301.1398788E-437.5000041
AAACACG5700.037.540
Produced by FastQC (version 0.11.5)