##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549957_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1083736
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.42235655178014	33.0	31.0	34.0	31.0	34.0
2	32.58686801951767	34.0	31.0	34.0	31.0	34.0
3	32.59235644105206	34.0	31.0	34.0	31.0	34.0
4	36.102907903769925	37.0	35.0	37.0	35.0	37.0
5	35.98176677714868	37.0	35.0	37.0	35.0	37.0
6	35.942018166786006	37.0	35.0	37.0	35.0	37.0
7	36.1114173562565	37.0	35.0	37.0	35.0	37.0
8	36.07755763396251	37.0	35.0	37.0	35.0	37.0
9	37.653147999143705	39.0	38.0	39.0	35.0	39.0
10	37.45757546118243	39.0	37.0	39.0	35.0	39.0
11	37.358746041471356	39.0	37.0	39.0	35.0	39.0
12	37.1464609462083	39.0	37.0	39.0	34.0	39.0
13	37.07778001284446	39.0	37.0	39.0	33.0	39.0
14	38.25529003373515	40.0	37.0	41.0	34.0	41.0
15	38.3319876796563	40.0	37.0	41.0	34.0	41.0
16	38.34245978725446	40.0	37.0	41.0	34.0	41.0
17	38.33134545682712	40.0	37.0	41.0	34.0	41.0
18	38.30882705751216	40.0	37.0	41.0	34.0	41.0
19	38.245046764156584	40.0	37.0	41.0	34.0	41.0
20	38.21623716477075	40.0	37.0	41.0	34.0	41.0
21	38.17843552304251	40.0	36.0	41.0	34.0	41.0
22	38.0834649767102	40.0	36.0	41.0	34.0	41.0
23	37.83250902433803	40.0	36.0	41.0	33.0	41.0
24	37.646633497456946	40.0	35.0	41.0	33.0	41.0
25	37.633678312799425	40.0	35.0	41.0	33.0	41.0
26	37.6935951191065	40.0	35.0	41.0	33.0	41.0
27	37.77056497154288	40.0	35.0	41.0	33.0	41.0
28	37.75347962972532	40.0	36.0	41.0	33.0	41.0
29	37.70944861110086	40.0	36.0	41.0	33.0	41.0
30	37.64542748418434	40.0	35.0	41.0	33.0	41.0
31	37.49499970472514	40.0	35.0	41.0	33.0	41.0
32	37.339314187219024	39.0	35.0	41.0	33.0	41.0
33	37.29066857611079	39.0	35.0	41.0	33.0	41.0
34	37.16187429410853	39.0	35.0	41.0	32.0	41.0
35	37.07203783947382	39.0	35.0	41.0	32.0	41.0
36	37.018918814176146	39.0	35.0	41.0	32.0	41.0
37	36.966660699653794	39.0	35.0	41.0	32.0	41.0
38	36.84517262506736	39.0	35.0	41.0	32.0	41.0
39	36.7528291022906	39.0	35.0	41.0	31.0	41.0
40	36.64710685997328	39.0	35.0	41.0	31.0	41.0
41	36.58183635128851	39.0	35.0	41.0	31.0	41.0
42	36.53823532668473	39.0	35.0	41.0	31.0	41.0
43	36.42301538382041	39.0	35.0	41.0	31.0	41.0
44	36.30802796991149	38.0	35.0	40.0	31.0	41.0
45	36.1961049554504	38.0	35.0	40.0	31.0	41.0
46	36.08199321606	38.0	35.0	40.0	31.0	41.0
47	35.95355972303218	38.0	35.0	40.0	30.0	41.0
48	35.88529217447792	38.0	35.0	40.0	30.0	41.0
49	35.84072135649272	37.0	35.0	40.0	30.0	41.0
50	35.74503384588129	37.0	35.0	40.0	30.0	41.0
51	34.73865406335122	36.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	11.0
10	15.0
11	10.0
12	17.0
13	16.0
14	23.0
15	32.0
16	73.0
17	135.0
18	275.0
19	496.0
20	842.0
21	1353.0
22	2067.0
23	3099.0
24	4366.0
25	5568.0
26	7251.0
27	8744.0
28	10256.0
29	12253.0
30	15239.0
31	19222.0
32	25455.0
33	36683.0
34	71942.0
35	98793.0
36	84452.0
37	130395.0
38	225573.0
39	318879.0
40	199.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.68776159507482	20.579919832874427	24.512150560653147	11.220168011397611
2	31.899466290683343	23.177600448817792	25.46422745022773	19.458705810271137
3	32.24678334945042	24.663109834867534	25.830737375154094	17.25936944052795
4	28.08202366628035	26.3575261871895	25.676271711929843	19.884178434600308
5	25.492555382491677	29.660267814301637	24.593074328065136	20.254102475141547
6	26.91817933518864	33.580779820915794	23.335387954261925	16.165652889633638
7	80.3630219905955	5.951633977278599	8.113045981678194	5.57229805044771
8	80.99269563805207	4.848228719909646	8.671392294802423	5.487683347235858
9	74.91833804542803	6.831276251780877	11.007939202905504	7.242446499885581
10	42.50979943454863	24.56160910037131	17.367606132858924	15.560985332221131
11	33.86092184812537	24.306565436600795	22.157702613920733	19.674810101353096
12	29.128311692146426	22.58575889330981	27.089438756302275	21.19649065824149
13	25.193220489122815	24.064532321524798	28.988702045516618	21.75354514383577
14	20.994042829619023	26.865675773435598	28.854905622771597	23.285375774173783
15	20.50859249854208	24.03629666265585	32.96522400289369	22.489886835908376
16	23.897148383001028	24.686916370776647	30.182350683192215	21.23358456303011
17	22.934829146581826	24.261166926262483	27.733414779983317	25.070589147172374
18	25.378597739670916	23.42876862999845	27.823750433684957	23.36888319664568
19	24.91861486561303	26.620228542744726	25.70884422036363	22.752312371278613
20	28.358105664110077	25.543490296529782	25.101777554681213	20.996626484678927
21	25.72379251035308	27.208655982637836	26.384746838713486	20.6828046682956
22	24.51999379922786	24.23302354078853	26.166520259546605	25.08046240043701
23	24.35620852310895	26.29930167494667	26.41981072881218	22.9246790731322
24	24.810654993467043	23.36445407368584	27.845619228299146	23.979271704547973
25	23.50830829648549	25.268146485860026	25.806654019059994	25.41689119859449
26	22.599599902559294	26.19429455144057	26.718407435021074	24.487698110979057
27	23.070655584016773	25.004429122959838	27.357862062347287	24.567053230676105
28	20.243767854901932	27.4946112337322	27.619364863767558	24.642256047598305
29	23.15942259000347	27.140373670340377	26.435774026146593	23.264429713509564
30	24.80188902094237	24.696881897436278	27.970649678519493	22.530579403101864
31	25.27312924918984	26.7124096643463	25.207707412137275	22.80675367432659
32	24.9032052086486	27.00408586593045	25.384687783740688	22.708021141680263
33	25.250522267415683	25.654679737500647	25.4326699491389	23.66212804594477
34	22.59470941262448	27.085101906737435	28.154919648327635	22.16526903231045
35	21.926096392479348	27.89387821388235	26.472406563960227	23.707618829678076
36	23.52279521949995	29.70806543290986	25.710228321288582	21.058911026301608
37	23.50590918821558	28.562952601002458	26.15350971085209	21.77762849992987
38	23.31398052662272	28.500206692404795	24.861128540530167	23.324684240442323
39	26.13247137679287	25.824093690714346	23.654653900950045	24.388781031542734
40	25.242309935260987	24.61964906582415	26.744982172780084	23.393058826134776
41	21.195567924291524	26.262207770158046	25.33255331556763	27.209670989982797
42	24.448851011685502	24.615496763049304	25.806654019059994	25.1289982062052
43	24.018211077236522	23.947898750249138	26.855064333010993	25.17882583950335
44	23.210542050831567	24.764056928993778	26.298471214391693	25.726929805782962
45	23.921693106070112	23.708633837023037	26.339532875165165	26.03014018174168
46	23.638506056825648	25.135549617249957	26.546871193722453	24.67907313220194
47	21.38952660057431	25.73763351960256	28.47418559501576	24.39865428480737
48	22.519691142492267	24.32179054677523	28.490148892350163	24.668369418382337
49	24.762580554673832	23.175754980917862	28.483966574885393	23.57769788952291
50	21.681571895738447	23.75910738408616	28.41909837820281	26.140222341972585
51	21.58680711907697	23.2607387777097	26.661474750308194	28.49097935290514
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	541.0
1	1179.0
2	1817.0
3	3176.0
4	4535.0
5	3283.5
6	2032.0
7	2047.0
8	2062.0
9	2096.5
10	2131.0
11	2136.0
12	2141.0
13	2264.5
14	2388.0
15	2434.5
16	2481.0
17	2521.5
18	2562.0
19	2420.0
20	2278.0
21	2776.0
22	3274.0
23	3512.0
24	3750.0
25	4143.0
26	5623.5
27	6711.0
28	8036.0
29	9361.0
30	10854.5
31	12348.0
32	14567.5
33	16787.0
34	18418.5
35	20050.0
36	22203.5
37	24357.0
38	27601.5
39	30846.0
40	34850.5
41	38855.0
42	47453.0
43	56051.0
44	67030.5
45	78010.0
46	90728.5
47	103447.0
48	102802.0
49	102157.0
50	92176.0
51	82195.0
52	76740.0
53	71285.0
54	67690.5
55	64096.0
56	61690.0
57	59284.0
58	56271.5
59	53259.0
60	54040.0
61	54821.0
62	51577.5
63	48334.0
64	42246.5
65	36159.0
66	30120.5
67	24082.0
68	20710.0
69	17338.0
70	15011.0
71	12684.0
72	10600.0
73	8516.0
74	7631.5
75	5533.0
76	4319.0
77	3518.0
78	2717.0
79	2010.5
80	1304.0
81	904.5
82	505.0
83	423.0
84	341.0
85	263.0
86	185.0
87	108.5
88	32.0
89	22.0
90	12.0
91	9.5
92	7.0
93	5.5
94	4.0
95	3.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1083736.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.188739405735475
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.63502202875314	20.193009682926427
2	10.823453433774691	6.101990166295752
3	3.989530726554491	3.373795260060572
4	2.0030283287648833	2.2585137432743654
5	1.1685556672119453	1.6470055592066435
6	0.8360791763082603	1.414081081410931
7	0.6383586035393718	1.2596167025806393
8	0.549232756034384	1.238574322635771
9	0.44136517806311	1.119737518846834
>10	7.546264080503609	50.602903437255655
>50	0.29635126359230884	5.162571470951108
>100	0.0667785850998528	3.554091385934016
>500	0.003986781199981811	0.783492188002246
>1k	0.0019933905999909053	1.2906174806190476
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGCT	3957	0.3651258240014173	No Hit
GCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC	2961	0.2732215225848362	No Hit
CCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC	2383	0.2198875002768202	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2019	0.18629998449806964	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCC	1311	0.1209704208404999	No Hit
TCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC	1150	0.10611440424605255	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8454678999313485E-4	0.0	0.0	0.3987133397801679	0.0
2	1.8454678999313485E-4	0.0	0.0	1.1539710778270722	0.0
3	1.8454678999313485E-4	0.0	0.0	1.6621206640731692	0.0
4	1.8454678999313485E-4	0.0	0.0	2.2157610340525737	0.0
5	1.8454678999313485E-4	0.0	0.0	3.51478588881425	0.0
6	1.8454678999313485E-4	0.0	0.0	4.379479873327083	0.0
7	1.8454678999313485E-4	0.0	0.0	5.225811452235599	0.0
8	1.8454678999313485E-4	0.0	0.0	6.631504351613308	0.0
9	1.8454678999313485E-4	0.0	0.0	7.185882908752685	0.0
10	2.7682018498970226E-4	0.0	0.0	8.264466622867563	0.0
11	2.7682018498970226E-4	0.0	0.0	9.81401374504492	0.0
12	2.7682018498970226E-4	0.0	0.0	10.983948120206398	0.0
13	2.7682018498970226E-4	0.0	0.0	11.471520739368259	0.0
14	2.7682018498970226E-4	0.0	0.0	11.672123100090797	0.0
15	2.7682018498970226E-4	0.0	0.0	12.067514597651089	0.0
16	2.7682018498970226E-4	0.0	0.0	12.801734001638776	0.0
17	2.7682018498970226E-4	0.0	0.0	13.654986085172034	0.0
18	2.7682018498970226E-4	0.0	0.0	14.620903983996103	0.0
19	2.7682018498970226E-4	0.0	0.0	15.217820576228897	0.0
20	2.7682018498970226E-4	0.0	0.0	15.823595414381362	0.0
21	2.7682018498970226E-4	0.0	0.0	16.548033838499414	0.0
22	2.7682018498970226E-4	0.0	0.0	17.25992308089793	0.0
23	3.690935799862697E-4	0.0	0.0	17.92170786981331	0.0
24	3.690935799862697E-4	0.0	0.0	18.50939712254645	0.0
25	3.690935799862697E-4	0.0	0.0	19.03360227952195	0.0
26	3.690935799862697E-4	0.0	0.0	19.544796887802935	0.0
27	3.690935799862697E-4	0.0	0.0	19.99813607742107	0.0
28	3.690935799862697E-4	0.0	0.0	20.47795773140322	0.0
29	3.690935799862697E-4	0.0	0.0	21.0027165287487	0.0
30	3.690935799862697E-4	0.0	0.0	21.591236242036807	0.0
31	3.690935799862697E-4	0.0	0.0	22.099016734702918	0.0
32	3.690935799862697E-4	0.0	0.0	22.604213572309124	0.0
33	3.690935799862697E-4	0.0	0.0	23.117899562255015	0.0
34	3.690935799862697E-4	0.0	0.0	23.584802940937646	0.0
35	3.690935799862697E-4	0.0	0.0	24.094428901503687	0.0
36	3.690935799862697E-4	0.0	0.0	24.571297806845948	0.0
37	3.690935799862697E-4	0.0	0.0	25.077048284822133	0.0
38	3.690935799862697E-4	0.0	0.0	25.610019414322306	0.0
39	3.690935799862697E-4	0.0	0.0	26.17962308163612	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTACG	30	2.1653868E-6	45.000004	1
CGGTCAT	20	7.0331065E-4	45.000004	19
GCGTATG	70	0.0	41.785717	1
ATTAGCG	60	3.6379788E-12	41.250004	1
TAACACG	60	3.6379788E-12	41.250004	1
TACGGGA	310	0.0	39.919353	4
AATAGGC	40	3.4579716E-7	39.375004	14
TACGGGT	75	0.0	39.0	4
AACACGT	540	0.0	38.750004	41
TATAGCG	35	6.2479576E-6	38.57143	1
CGAGGGT	170	0.0	38.38235	4
AATGCGA	65	9.094947E-12	38.07692	25
ACACGAC	595	0.0	37.815125	26
ACGACCA	590	0.0	37.75424	28
ATAGACG	60	1.5643309E-10	37.500004	1
CGCGAGT	30	1.1398788E-4	37.500004	16
TCGTCCA	30	1.1398788E-4	37.500004	14
TCAACGG	60	1.5643309E-10	37.500004	2
TAGTACG	30	1.1398788E-4	37.500004	1
AAACACG	570	0.0	37.5	40
>>END_MODULE