##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549956_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	724881
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.48776419853741	33.0	31.0	34.0	31.0	34.0
2	32.67300287909326	34.0	31.0	34.0	31.0	34.0
3	32.64191915638567	34.0	31.0	34.0	31.0	34.0
4	36.10004952537037	37.0	35.0	37.0	35.0	37.0
5	36.11965274300196	37.0	35.0	37.0	35.0	37.0
6	36.110440196390854	37.0	35.0	37.0	35.0	37.0
7	36.207969308065735	37.0	36.0	37.0	35.0	37.0
8	36.20494674298264	37.0	37.0	37.0	35.0	37.0
9	37.60533108192931	39.0	38.0	39.0	35.0	39.0
10	37.63870069707993	39.0	37.0	39.0	35.0	39.0
11	37.582732889950215	39.0	37.0	39.0	35.0	39.0
12	37.40999557168694	39.0	37.0	39.0	35.0	39.0
13	37.33034939528005	39.0	37.0	39.0	35.0	39.0
14	38.52175736431221	40.0	38.0	41.0	35.0	41.0
15	38.55141878460051	40.0	38.0	41.0	35.0	41.0
16	38.577709996537365	40.0	38.0	41.0	35.0	41.0
17	38.49911502715618	40.0	38.0	41.0	34.0	41.0
18	38.43343528110131	40.0	37.0	41.0	34.0	41.0
19	38.41148823048197	40.0	37.0	41.0	34.0	41.0
20	38.35135836088958	40.0	37.0	41.0	34.0	41.0
21	38.330763256313794	40.0	37.0	41.0	34.0	41.0
22	38.28697951801744	40.0	37.0	41.0	34.0	41.0
23	38.26726455790675	40.0	37.0	41.0	34.0	41.0
24	38.24257774724403	40.0	36.0	41.0	34.0	41.0
25	38.15254779750055	40.0	36.0	41.0	34.0	41.0
26	38.07529097879514	40.0	36.0	41.0	34.0	41.0
27	37.9956351456308	40.0	36.0	41.0	34.0	41.0
28	37.892702388392024	40.0	36.0	41.0	34.0	41.0
29	37.824455324391174	40.0	36.0	41.0	34.0	41.0
30	37.706397325905904	40.0	36.0	41.0	33.0	41.0
31	37.56091413625133	40.0	35.0	41.0	33.0	41.0
32	37.47069518996911	40.0	35.0	41.0	33.0	41.0
33	37.338724563066215	40.0	35.0	41.0	33.0	41.0
34	37.14052651400713	40.0	35.0	41.0	32.0	41.0
35	37.09195026494004	40.0	35.0	41.0	32.0	41.0
36	36.99912123507169	39.0	35.0	41.0	32.0	41.0
37	36.869799318784736	39.0	35.0	41.0	32.0	41.0
38	36.73009500869798	39.0	35.0	41.0	31.0	41.0
39	36.605904969229435	39.0	35.0	41.0	31.0	41.0
40	36.4430520319887	39.0	35.0	41.0	31.0	41.0
41	36.34572709175713	39.0	35.0	41.0	31.0	41.0
42	36.21597475999509	39.0	35.0	41.0	30.0	41.0
43	36.148309860515035	38.0	35.0	40.0	30.0	41.0
44	36.045760614500864	38.0	35.0	40.0	30.0	41.0
45	35.931907444118416	38.0	35.0	40.0	30.0	41.0
46	35.803847803984375	38.0	35.0	40.0	30.0	41.0
47	35.72447615539654	38.0	35.0	40.0	29.0	41.0
48	35.59551429820895	38.0	35.0	40.0	29.0	41.0
49	35.50457247465446	38.0	35.0	40.0	29.0	41.0
50	35.37025939430058	37.0	35.0	40.0	28.0	41.0
51	33.69713511597076	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	16.0
10	23.0
11	19.0
12	32.0
13	27.0
14	36.0
15	45.0
16	82.0
17	115.0
18	221.0
19	383.0
20	662.0
21	1062.0
22	1527.0
23	2358.0
24	3380.0
25	4550.0
26	5499.0
27	6354.0
28	7139.0
29	8411.0
30	9997.0
31	12419.0
32	15975.0
33	22461.0
34	46918.0
35	64851.0
36	50051.0
37	77915.0
38	145175.0
39	237072.0
40	98.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.07945855940493	21.50008070290158	24.448288753602316	11.972171984091181
2	32.63349432527546	24.84131878197939	24.39779770748578	18.127389185259375
3	31.5404873351626	24.996102808598927	26.037791030527767	17.425618825710703
4	28.160208365235125	25.67759397749424	26.018891376653546	20.143306280617093
5	25.055284936424048	29.717705388884518	24.124787378893913	21.102222295797517
6	25.563230378503505	34.21554710359356	24.215836806317174	16.005385711585767
7	78.5675165992763	6.964177568456063	9.138189578703262	5.330116253564378
8	78.78727680819334	5.682863807990553	9.373124692190856	6.156734691625246
9	72.43809673587802	7.452671541949645	12.122955354051216	7.9862763681211115
10	40.888366504295185	23.663746187305225	18.68941246908113	16.758474839318456
11	31.759419822012163	24.433803617421344	23.650778541581307	20.155998018985187
12	28.790794626980155	20.54268217817821	29.367992815372457	21.298530379469184
13	25.14054030937492	22.40464296898387	31.442126362809898	21.012690358831314
14	20.43852715135312	26.677758142370955	30.535770698914718	22.34794400736121
15	19.84863722459273	24.773445572445684	34.36012255804746	21.01779464491413
16	23.11855325218898	23.840188941357273	30.87996512531022	22.161292681143525
17	22.06113831097794	23.762245113335844	27.974936575796576	26.201679999889638
18	23.597666375584406	25.077909339601952	28.880050656590527	22.444373628223115
19	24.583207450602238	27.151491072327733	25.838999780653655	22.426301696416377
20	25.677456023816326	26.256033748987768	25.65786660155253	22.408643625643382
21	25.28346031969385	26.940008084085527	26.988291871355436	20.788239724865186
22	22.017820856112934	25.573438950669143	26.47910484617475	25.929635347043167
23	22.763874346272008	25.516739989046478	27.009536737754196	24.709848926927318
24	25.181788459071214	23.24146997921038	27.508515190769245	24.068226370949162
25	23.29996233864593	24.35530797468826	25.663936563380744	26.680793123285063
26	20.702984351914314	25.449556547902347	27.152456748073135	26.695002352110208
27	22.091488120119028	24.558099881221885	28.148068441578687	25.202343557080404
28	20.090607975653935	25.80326977807392	28.519163835167426	25.58695841110472
29	22.173984419511616	24.637561199700365	26.96870244909164	26.219751931696372
30	23.83425693320697	23.21346538259383	28.96654761264263	23.985730071556574
31	23.764038511148726	25.530949217871623	26.671964087898566	24.033048183081085
32	24.50250454902253	24.331441988409132	25.31118900895457	25.854864453613764
33	24.332959478866186	23.326863305839165	27.563972569290684	24.776204646003965
34	21.232450567748362	24.50043524385382	29.082704609446242	25.184409578951577
35	20.611245156101486	23.87109056521001	27.504238626753907	28.013425651934593
36	22.727868436336447	25.91432248879471	28.206560801014234	23.151248273854605
37	22.54342436896539	26.110354665110545	28.07660843641922	23.269612529504844
38	22.144600286115928	26.453859323116486	25.478664773942207	25.92287561682538
39	24.387865042675973	24.020632352068823	26.119873468886617	25.47162913636859
40	23.43308763783297	22.375396789266098	29.044491440664054	25.14702413223688
41	20.82921196720565	24.491744162145235	27.215639532557756	27.463404338091358
42	22.63254244489785	23.487027525897357	26.876687345922985	27.003742683281807
43	22.981703203698263	21.887868491517917	28.919781315829773	26.21064698895405
44	21.840274472637578	22.717797817848723	28.972065759759186	26.469861949754513
45	22.654752987042013	22.171639206987077	27.84663965533653	27.32696815063438
46	22.957009495351652	23.14973078339755	28.668981529382066	25.22427819186873
47	21.145953611696264	23.045161895538715	30.202336659396504	25.60654783336851
48	21.443105833923084	22.171225345953335	29.799649873565453	26.586018946558127
49	23.14766147822884	20.8512845556719	30.13611889399777	25.864935072101492
50	20.532611559690487	21.494838463140848	30.382228255396406	27.590321721772266
51	20.479085532659845	21.611823182011943	28.577242333569234	29.331848951758978
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1108.0
1	1199.5
2	1291.0
3	2821.5
4	4352.0
5	3065.5
6	1779.0
7	1646.0
8	1513.0
9	1656.0
10	1799.0
11	1928.5
12	2058.0
13	2052.5
14	2047.0
15	2017.5
16	1988.0
17	1956.0
18	1924.0
19	1883.5
20	1843.0
21	2048.0
22	2253.0
23	2280.5
24	2308.0
25	2711.0
26	3814.0
27	4514.0
28	5508.5
29	6503.0
30	7398.0
31	8293.0
32	9187.0
33	10081.0
34	10981.0
35	11881.0
36	12782.0
37	13683.0
38	15901.0
39	18119.0
40	21192.0
41	24265.0
42	27909.0
43	31553.0
44	36322.0
45	41091.0
46	51203.0
47	61315.0
48	69387.0
49	77459.0
50	76803.5
51	76148.0
52	65061.5
53	53975.0
54	48983.5
55	43992.0
56	41106.5
57	38221.0
58	36900.5
59	35580.0
60	35397.0
61	35214.0
62	32977.0
63	30740.0
64	26745.0
65	22750.0
66	19111.0
67	15472.0
68	13246.0
69	11020.0
70	9410.5
71	7801.0
72	6893.0
73	5985.0
74	5225.5
75	3388.5
76	2311.0
77	1932.0
78	1553.0
79	1102.5
80	652.0
81	536.0
82	420.0
83	307.5
84	195.0
85	157.5
86	120.0
87	94.0
88	68.0
89	47.0
90	26.0
91	23.5
92	21.0
93	14.5
94	8.0
95	6.0
96	4.0
97	4.0
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	724881.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.230554591181246
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.93222252056597	23.5064597940528
2	10.477473926578666	6.753895907365435
3	3.9418200933444414	3.8114114312145952
4	2.0051127385573975	2.5850358232618853
5	1.2772110249458697	2.05826098319882
6	0.9486508040106647	1.8345324915980226
7	0.6900470526050039	1.5568419439628505
8	0.5952424197413482	1.5347994643568275
9	0.5142257078962434	1.4916401775484824
>10	6.295873346755129	44.04851146073058
>50	0.26191095103120837	5.256484367841302
>100	0.052468203600773027	2.8923000560092937
>500	0.005590874154180733	1.1888841934434828
>1k	0.0021503362131464355	1.4809419054156665
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTGC	2713	0.37426832818076344	No Hit
CCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTGC	2582	0.3561963963740255	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2310	0.31867299598140936	No Hit
CTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTGCT	1865	0.257283609309666	No Hit
CGCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTG	1214	0.16747576498763245	No Hit
TCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTGC	919	0.1267794300029936	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTC	897	0.12374444908888493	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	878	0.12112332920851837	No Hit
ACTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTGC	738	0.10180981430055416	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.7590735582806004E-4	0.0	0.0	0.284874344892472	0.0
2	2.7590735582806004E-4	0.0	0.0	1.3537394413703767	0.0
3	2.7590735582806004E-4	0.0	0.0	1.974944853017254	0.0
4	2.7590735582806004E-4	0.0	0.0	2.5906321175475697	0.0
5	2.7590735582806004E-4	0.0	0.0	4.293118456684614	0.0
6	2.7590735582806004E-4	0.0	0.0	5.502558350956916	0.0
7	2.7590735582806004E-4	0.0	0.0	6.642331637882632	0.0
8	2.7590735582806004E-4	0.0	0.0	8.399033772439891	0.0
9	2.7590735582806004E-4	0.0	0.0	9.194198771936358	0.0
10	2.7590735582806004E-4	0.0	0.0	10.629468836953928	0.0
11	2.7590735582806004E-4	0.0	0.0	12.359683865351693	0.0
12	2.7590735582806004E-4	0.0	0.0	13.721562573718996	0.0
13	2.7590735582806004E-4	0.0	0.0	14.283309950184927	0.0
14	2.7590735582806004E-4	0.0	0.0	14.516037804825896	0.0
15	2.7590735582806004E-4	0.0	0.0	14.919276405368604	0.0
16	2.7590735582806004E-4	0.0	0.0	15.695541750990852	0.0
17	2.7590735582806004E-4	0.0	0.0	16.58037664113144	0.0
18	2.7590735582806004E-4	0.0	0.0	17.627031195465186	0.0
19	2.7590735582806004E-4	0.0	0.0	18.24023529379305	0.0
20	2.7590735582806004E-4	0.0	0.0	18.84295491259945	0.0
21	2.7590735582806004E-4	0.0	0.0	19.52568766459598	0.0
22	2.7590735582806004E-4	0.0	0.0	20.20069501062933	0.0
23	2.7590735582806004E-4	0.0	0.0	20.877909615509303	0.0
24	2.7590735582806004E-4	0.0	0.0	21.425723670505917	0.0
25	2.7590735582806004E-4	0.0	0.0	21.90607837700257	0.0
26	2.7590735582806004E-4	0.0	0.0	22.360497792051383	0.0
27	2.7590735582806004E-4	0.0	0.0	22.819883539505106	0.0
28	2.7590735582806004E-4	0.0	0.0	23.306308207829975	0.0
29	2.7590735582806004E-4	0.0	0.0	23.80556256820085	0.0
30	2.7590735582806004E-4	0.0	0.0	24.41421419515755	0.0
31	2.7590735582806004E-4	0.0	0.0	24.91015766725849	0.0
32	2.7590735582806004E-4	0.0	0.0	25.386511717095633	0.0
33	2.7590735582806004E-4	0.0	0.0	25.905907314441958	0.0
34	2.7590735582806004E-4	0.0	0.0	26.366948506030646	0.0
35	2.7590735582806004E-4	0.0	0.0	26.860684719284958	0.0
36	2.7590735582806004E-4	0.0	0.0	27.349592553812283	0.0
37	2.7590735582806004E-4	0.0	0.0	27.818221197686242	0.0
38	2.7590735582806004E-4	0.0	0.0	28.339134285489617	0.0
39	2.7590735582806004E-4	0.0	0.0	28.891500811857394	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCATAA	20	7.0315605E-4	45.0	21
GCACGAT	40	6.8084773E-9	45.0	41
GTTAGAA	20	7.0315605E-4	45.0	9
TAACGCG	35	1.2111013E-7	45.0	1
TCGTACA	20	7.0315605E-4	45.0	1
CGTAAAT	20	7.0315605E-4	45.0	15
AATACCG	20	7.0315605E-4	45.0	1
ATCACGC	30	2.1643882E-6	44.999996	45
AATAGCG	50	1.0804797E-9	40.5	1
GATGCGA	45	1.9266736E-8	40.0	11
GCTAACG	45	1.9266736E-8	40.0	1
AGGGTAC	215	0.0	38.720932	6
ATAGCGG	140	0.0	38.571426	2
ATAGACG	35	6.2450963E-6	38.571426	1
ATATGCG	35	6.2450963E-6	38.571426	1
AAGCACG	35	6.2450963E-6	38.571426	1
GCGATAT	35	6.2450963E-6	38.571426	9
TTATTCG	35	6.2450963E-6	38.571426	13
CAATAGG	100	0.0	38.25	2
CACAACG	155	0.0	37.741936	12
>>END_MODULE