FastQCFastQC Report
Sat 18 Jun 2016
SRR3549954_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549954_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138566
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGC18971.3690227039822178No Hit
GCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGC14411.0399376470418429No Hit
CTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGCT12130.8753951185716554No Hit
TCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGC5940.4286765873302253No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCC4500.3247549904016859No Hit
ACTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGC4470.32258995713234123No Hit
CGCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTG3740.2699074809116233No Hit
GAATCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTC3710.2677424476422788No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3650.2634123811035896No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3110.22444178225538733No Hit
CGTTCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTC2830.20423480507483796No Hit
GCCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTG2690.1941313164845633No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCG2470.1782544058427031No Hit
AAAACTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTC2450.17681105032980673No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGC2430.17536769481691036No Hit
ACCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTG2040.14722226231543092No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCT2000.14433555128963815No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTC1810.1306236739171225No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA1770.12773696289132977No Hit
AGCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTG1720.1241285741090888No Hit
GAAACTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTC1680.12124186308329606No Hit
GGCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTG1670.12052018532684786No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCG1630.11763347430105509No Hit
GGCCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCT1570.11330340776236594No Hit
AGCCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCT1550.11186005224946957No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1540.11113837449302137No Hit
AAAAACTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTT1520.109695018980125No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG1390.10031320814629852No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGA207.0118025E-445.013
TTGATAG253.8730086E-545.031
TGTTTAG253.8730086E-545.01
CAACGAG207.0118025E-445.014
CATGAGG207.0118025E-445.02
ATCAGGA207.0118025E-445.016
TCGGGAT253.8730086E-545.05
ACACAAC207.0118025E-445.011
GCACGGG351.2016062E-745.03
AGGGTCC207.0118025E-445.06
TAGCCGG207.0118025E-445.02
TCAGGAC207.0118025E-445.017
GTTAATA253.8730086E-545.037
TAATTGG253.8730086E-545.02
GGGAAGC207.0118025E-445.07
CGTCGGG207.0118025E-445.03
TACTAGG207.0118025E-445.02
TAAGGGT207.0118025E-445.04
TCGAGCG207.0118025E-445.01
TAGTGGG253.8730086E-545.03
Produced by FastQC (version 0.11.5)