##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549954_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	138566
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.166454974524775	33.0	31.0	34.0	30.0	34.0
2	32.341447396908336	34.0	31.0	34.0	30.0	34.0
3	32.34896727913052	34.0	31.0	34.0	30.0	34.0
4	35.87198879956122	37.0	35.0	37.0	35.0	37.0
5	35.90098581181531	37.0	35.0	37.0	35.0	37.0
6	35.896403158061865	37.0	35.0	37.0	35.0	37.0
7	36.021065773710724	37.0	35.0	37.0	35.0	37.0
8	35.967805955284845	37.0	35.0	37.0	35.0	37.0
9	37.2574368892802	39.0	37.0	39.0	34.0	39.0
10	37.39127924599108	39.0	37.0	39.0	34.0	39.0
11	37.431491130580376	39.0	37.0	39.0	35.0	39.0
12	37.50445996853485	39.0	37.0	39.0	35.0	39.0
13	37.4925234184432	39.0	37.0	39.0	35.0	39.0
14	38.6102074101872	40.0	38.0	41.0	35.0	41.0
15	38.60101323557005	40.0	38.0	41.0	35.0	41.0
16	38.609160977440354	40.0	38.0	41.0	35.0	41.0
17	38.523259674090326	40.0	38.0	41.0	34.0	41.0
18	38.44454628119452	40.0	38.0	41.0	34.0	41.0
19	38.431296277586135	40.0	38.0	41.0	34.0	41.0
20	38.407286058629104	40.0	38.0	41.0	34.0	41.0
21	38.411219202401746	40.0	38.0	41.0	34.0	41.0
22	38.397911464572836	40.0	38.0	41.0	34.0	41.0
23	38.39842385577992	40.0	38.0	41.0	34.0	41.0
24	38.36544318231024	40.0	38.0	41.0	34.0	41.0
25	38.3205259587489	40.0	37.0	41.0	34.0	41.0
26	38.320424923862994	40.0	38.0	41.0	34.0	41.0
27	38.25563991166664	40.0	37.0	41.0	34.0	41.0
28	38.13956526131952	40.0	37.0	41.0	34.0	41.0
29	37.99464515104715	40.0	37.0	41.0	34.0	41.0
30	37.827908722197364	40.0	37.0	41.0	33.0	41.0
31	37.558217744612676	40.0	36.0	41.0	33.0	41.0
32	37.31623197609803	39.0	36.0	41.0	32.0	41.0
33	37.25002525872148	40.0	36.0	41.0	32.0	41.0
34	37.03694268435258	39.0	36.0	41.0	31.0	41.0
35	36.888767807398644	39.0	35.0	41.0	31.0	41.0
36	36.83950608374349	39.0	36.0	41.0	31.0	41.0
37	36.622079009280775	39.0	35.0	41.0	30.0	41.0
38	36.4029776424231	39.0	35.0	41.0	30.0	41.0
39	36.3248127246222	39.0	35.0	40.0	30.0	41.0
40	36.17753994486382	39.0	35.0	40.0	30.0	41.0
41	36.140676645064445	39.0	35.0	40.0	29.0	41.0
42	36.146060361127546	39.0	35.0	40.0	30.0	41.0
43	36.09819147554234	39.0	35.0	40.0	29.0	41.0
44	35.93670164398193	39.0	35.0	40.0	29.0	41.0
45	35.8038840696852	39.0	35.0	40.0	28.0	41.0
46	35.594590303537665	39.0	35.0	40.0	26.0	41.0
47	35.526846412539875	38.0	35.0	40.0	26.0	41.0
48	35.37944373078533	38.0	35.0	40.0	26.0	41.0
49	35.30897911464573	38.0	35.0	40.0	26.0	41.0
50	35.14737381464428	38.0	34.0	40.0	24.0	41.0
51	33.103596841938135	36.0	31.0	39.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	2.0
12	2.0
13	4.0
14	4.0
15	10.0
16	9.0
17	13.0
18	29.0
19	56.0
20	110.0
21	181.0
22	332.0
23	532.0
24	928.0
25	1189.0
26	1247.0
27	1440.0
28	1557.0
29	1887.0
30	2144.0
31	2617.0
32	3461.0
33	4454.0
34	6834.0
35	8873.0
36	10231.0
37	16580.0
38	32844.0
39	40983.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.6268492992509	22.70398221786008	23.818252673816087	12.850915809072932
2	29.89910944964854	27.687888803891287	23.827634484649916	18.585367261810255
3	28.784117315936086	26.63640431274627	28.00903540551073	16.570442965806908
4	27.785315301011792	26.551968015241833	26.764141275637606	18.898575408108773
5	22.29046086341527	29.31382878916906	26.26185355714966	22.13385679026601
6	22.076122569750154	33.993187361979125	27.301069526435057	16.62962054183566
7	69.67365731853413	8.569923357822265	14.276229378058108	7.480189945585497
8	65.49514310869911	8.655081333083151	14.761196830391293	11.088578727826452
9	58.89467834822395	10.366179293621812	18.590419006105392	12.148723352048844
10	27.788202012037583	29.907047904969474	24.99458741682664	17.310162666166303
11	20.624106923776395	25.71121342897969	30.341497914351283	23.32318173289263
12	19.33013870646479	21.92745695192183	37.28692464240867	21.45547969920471
13	20.065528340285496	25.14902645670655	37.094236681437	17.691208521570946
14	16.08691886898662	29.435792330008802	31.77763664968318	22.69965215132139
15	14.51149632666022	28.427608504250685	36.92247737540234	20.138417793686763
16	15.101107053678392	29.86086052855679	30.51470057589885	24.523331841865968
17	15.24472092721158	30.14375820908448	28.568335666758077	26.043185196945863
18	14.663770333270786	28.753806850165265	34.5842414445102	21.99818137205375
19	15.16605805175873	29.34919099923502	30.57820821846629	24.90654273053996
20	16.416003925926994	31.230604910295455	31.32153630760793	21.031854856169623
21	17.642856111888918	31.097816203108984	32.15146572752335	19.107861957478747
22	14.121790338178197	29.966947158754675	28.066769626026588	27.844492877040544
23	15.271423004200166	28.718444640099307	29.99292755798681	26.017204797713724
24	20.18821355888169	27.0037382907784	28.055944459679864	24.752103690660046
25	15.919489629490641	30.835125499761844	25.141809679142067	28.103575191605444
26	14.567787191663179	28.815871137219805	27.62293780581095	28.99340386530606
27	20.10089055035146	26.494955472482427	26.43722125196657	26.966932725199545
28	17.403980774504568	25.646262430899352	27.55510009670482	29.394656697891257
29	17.921423725877922	23.536798348801295	27.825729255372888	30.71604866994789
30	23.057604318519694	24.338582336215232	25.15696491202748	27.44684843323759
31	21.848072398712524	27.149517197580934	25.022011171571666	25.980399232134864
32	18.536293174371778	27.933980918840117	26.859402739488765	26.670323167299337
33	22.089112769366224	23.228641946797914	28.632564987081967	26.04968029675389
34	19.292611463129482	21.620743905431347	32.857266573329674	26.229378058109493
35	17.22356133539252	24.123522364793672	28.84257321420839	29.810343085605417
36	22.100659613469396	22.657794841447394	30.38985032403331	24.851695221049898
37	19.40591487089185	23.370090787061763	35.96336763708269	21.260626704963702
38	20.1239842385578	27.04703895616529	30.528412453271365	22.30056435200554
39	23.890420449460905	20.19903872522841	35.29725906788101	20.61328175742967
40	22.56469841086558	21.713840336013163	33.98885729544044	21.732603957680816
41	16.79704978133164	25.973182454570388	29.712916588484912	27.516851175613066
42	19.255084219794178	22.519954389965793	33.04562446776265	25.179336922477376
43	23.61690457976704	19.256527575307075	33.29893336027597	23.827634484649916
44	21.640229204855448	19.387872926980645	30.77955631251534	28.19234155564857
45	21.793224889222465	17.500685593868624	30.417274078778345	30.288815438130566
46	24.67993591501523	19.530765122757387	31.93135401180665	23.857944950420737
47	17.27480045610034	20.501421705180203	37.76251028390803	24.461267554811425
48	17.215622880071592	19.842529913543004	33.97370206255503	28.968145143830377
49	20.991440901808524	16.740037238572235	38.052624742000205	24.21589711761904
50	20.777102608143412	15.64525208204033	35.234473103070016	28.343172206746242
51	18.324119913976013	16.01547277109825	30.73697732488489	34.92342999004085
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	423.0
1	397.0
2	371.0
3	509.0
4	647.0
5	513.0
6	379.0
7	416.5
8	454.0
9	488.0
10	522.0
11	578.0
12	634.0
13	647.5
14	661.0
15	641.0
16	621.0
17	589.0
18	557.0
19	572.5
20	588.0
21	558.5
22	529.0
23	633.0
24	737.0
25	866.0
26	1104.5
27	1214.0
28	1407.0
29	1600.0
30	1769.5
31	1939.0
32	2051.0
33	2163.0
34	2373.0
35	2583.0
36	2804.0
37	3025.0
38	3347.5
39	3670.0
40	4230.0
41	4790.0
42	5695.0
43	6600.0
44	8212.0
45	9824.0
46	13332.0
47	16840.0
48	20085.0
49	23330.0
50	22504.5
51	21679.0
52	16704.0
53	11729.0
54	9191.5
55	6654.0
56	5323.0
57	3992.0
58	3405.0
59	2818.0
60	2412.0
61	2006.0
62	1680.5
63	1355.0
64	1222.5
65	1090.0
66	855.5
67	621.0
68	477.0
69	333.0
70	279.0
71	225.0
72	194.0
73	163.0
74	142.0
75	78.5
76	36.0
77	29.5
78	23.0
79	16.5
80	10.0
81	8.5
82	7.0
83	5.0
84	3.0
85	3.5
86	4.0
87	2.0
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	138566.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.91585237359814
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.48853932042928	48.2253943968939
2	10.600676920853259	12.702971869001054
3	3.7748575695891495	6.78521426612589
4	1.775411632920998	4.255012052018533
5	1.0177902508943304	3.049088520993606
6	0.648013201161124	2.3295757978147598
7	0.42397889741396966	1.778213991888342
8	0.26016886886766316	1.2470591631424737
9	0.19271768064271347	1.0392159692853948
>10	0.7154643893860737	7.344514527373238
>50	0.05179287667272924	2.1989521238976373
>100	0.04577044915264444	5.331755264639233
>500	0.0012044855040169592	0.4286765873302253
>1k	0.0036134565120508775	3.2843554695957162
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGC	1897	1.3690227039822178	No Hit
GCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGC	1441	1.0399376470418429	No Hit
CTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGCT	1213	0.8753951185716554	No Hit
TCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGC	594	0.4286765873302253	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCC	450	0.3247549904016859	No Hit
ACTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGC	447	0.32258995713234123	No Hit
CGCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTG	374	0.2699074809116233	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTC	371	0.2677424476422788	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	365	0.2634123811035896	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	311	0.22444178225538733	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTC	283	0.20423480507483796	No Hit
GCCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTG	269	0.1941313164845633	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCG	247	0.1782544058427031	No Hit
AAAACTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTC	245	0.17681105032980673	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGC	243	0.17536769481691036	No Hit
ACCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTG	204	0.14722226231543092	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCT	200	0.14433555128963815	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTC	181	0.1306236739171225	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	177	0.12773696289132977	No Hit
AGCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTG	172	0.1241285741090888	No Hit
GAAACTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTC	168	0.12124186308329606	No Hit
GGCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTG	167	0.12052018532684786	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCG	163	0.11763347430105509	No Hit
GGCCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCT	157	0.11330340776236594	No Hit
AGCCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCT	155	0.11186005224946957	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	154	0.11113837449302137	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTT	152	0.109695018980125	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	139	0.10031320814629852	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.216777564481908E-4	0.0	0.0	0.998802014924296	0.0
2	7.216777564481908E-4	0.0	0.0	4.578323686907322	0.0
3	7.216777564481908E-4	0.0	0.0	6.03106101063753	0.0
4	7.216777564481908E-4	0.0	0.0	7.836698757270903	0.0
5	7.216777564481908E-4	0.0	0.0	12.79967668836511	0.0
6	7.216777564481908E-4	0.0	0.0	15.414315199976906	0.0
7	7.216777564481908E-4	0.0	0.0	17.943795736327814	0.0
8	7.216777564481908E-4	0.0	0.0	21.63084739402162	0.0
9	7.216777564481908E-4	0.0	0.0	23.070594518135763	0.0
10	7.216777564481908E-4	0.0	0.0	26.184634037209705	0.0
11	7.216777564481908E-4	0.0	0.0	30.48655514339737	0.0
12	7.216777564481908E-4	0.0	0.0	33.833696577804076	0.0
13	7.216777564481908E-4	0.0	0.0	35.12838647287213	0.0
14	7.216777564481908E-4	0.0	0.0	35.66531472366959	0.0
15	7.216777564481908E-4	0.0	0.0	36.5970007072442	0.0
16	7.216777564481908E-4	0.0	0.0	38.39181328753085	0.0
17	7.216777564481908E-4	0.0	0.0	40.4651934818065	0.0
18	7.216777564481908E-4	0.0	0.0	42.63600017320266	0.0
19	7.216777564481908E-4	0.0	0.0	43.92996839051427	0.0
20	7.216777564481908E-4	0.0	0.0	45.16042896525843	0.0
21	7.216777564481908E-4	0.0	0.0	46.59728937834678	0.0
22	7.216777564481908E-4	0.0	0.0	48.05652180188502	0.0
23	7.216777564481908E-4	0.0	0.0	49.344716597145045	0.0
24	7.216777564481908E-4	0.0	0.0	50.43372833162536	0.0
25	7.216777564481908E-4	0.0	0.0	51.37551780379025	0.0
26	7.216777564481908E-4	0.0	0.0	52.23792272274584	0.0
27	7.216777564481908E-4	0.0	0.0	53.08805911984181	0.0
28	7.216777564481908E-4	0.0	0.0	53.971392693734394	0.0
29	7.216777564481908E-4	0.0	0.0	54.83884935698512	0.0
30	7.216777564481908E-4	0.0	0.0	55.730843063955085	0.0
31	7.216777564481908E-4	0.0	0.0	56.52757530707389	0.0
32	7.216777564481908E-4	0.0	0.0	57.31636909487176	0.0
33	7.216777564481908E-4	0.0	0.0	58.03371678478126	0.0
34	7.216777564481908E-4	0.0	0.0	58.70559877603453	0.0
35	7.216777564481908E-4	0.0	0.0	59.44315344312458	0.0
36	7.216777564481908E-4	0.0	0.0	60.16771791059856	0.0
37	0.0014433555128963815	0.0	0.0	60.81289782486324	0.0
38	0.0014433555128963815	0.0	0.0	61.42055049579262	0.0
39	0.0014433555128963815	0.0	0.0	62.03469826653003	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGA	20	7.0118025E-4	45.0	13
TTGATAG	25	3.8730086E-5	45.0	31
TGTTTAG	25	3.8730086E-5	45.0	1
CAACGAG	20	7.0118025E-4	45.0	14
CATGAGG	20	7.0118025E-4	45.0	2
ATCAGGA	20	7.0118025E-4	45.0	16
TCGGGAT	25	3.8730086E-5	45.0	5
ACACAAC	20	7.0118025E-4	45.0	11
GCACGGG	35	1.2016062E-7	45.0	3
AGGGTCC	20	7.0118025E-4	45.0	6
TAGCCGG	20	7.0118025E-4	45.0	2
TCAGGAC	20	7.0118025E-4	45.0	17
GTTAATA	25	3.8730086E-5	45.0	37
TAATTGG	25	3.8730086E-5	45.0	2
GGGAAGC	20	7.0118025E-4	45.0	7
CGTCGGG	20	7.0118025E-4	45.0	3
TACTAGG	20	7.0118025E-4	45.0	2
TAAGGGT	20	7.0118025E-4	45.0	4
TCGAGCG	20	7.0118025E-4	45.0	1
TAGTGGG	25	3.8730086E-5	45.0	3
>>END_MODULE