SRR3549952_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549952_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	919418
Sequences flagged as poor quality	0
Sequence length	51
%GC	50

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2518	0.2738689040240674	No Hit
CCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCTGC	2357	0.25635782636406945	RNA PCR Primer, Index 17 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCTGC	1988	0.21622374154084434	RNA PCR Primer, Index 47 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCTGCT	1442	0.15683834773737299	RNA PCR Primer, Index 17 (96% over 25bp)
CGCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCTG	1222	0.13291016708395964	RNA PCR Primer, Index 17 (95% over 23bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGTTA	20	7.032551E-4	45.0	16
CCGCTAA	20	7.032551E-4	45.0	40
CTCGAAT	25	3.8901948E-5	45.0	39
TTACGAG	40	6.8121153E-9	45.0	1
TATACGG	55	1.8189894E-12	45.0	2
TGCGTTA	20	7.032551E-4	45.0	13
AATGCGA	95	0.0	40.263157	25
TCGATTG	45	1.927583E-8	40.0	1
AATCGGT	45	1.927583E-8	40.0	26
CGTATGG	85	0.0	39.705883	2
TCGTTGA	40	3.4572076E-7	39.375	24
TCGTTAG	35	6.2469244E-6	38.57143	1
GTATACG	35	6.2469244E-6	38.57143	1
TTACGGG	175	0.0	38.571426	3
TAGCCGT	100	0.0	38.25	44
CGGTCTA	95	0.0	37.894737	31
TCGACGA	30	1.1397441E-4	37.500004	1
TACGTTG	30	1.1397441E-4	37.500004	1
TCACGAC	90	0.0	37.5	25
CTCACGA	90	0.0	37.5	24