##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549952_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	919418
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.582314029092316	34.0	31.0	34.0	31.0	34.0
2	32.76520799027211	34.0	31.0	34.0	31.0	34.0
3	32.743894507177366	34.0	31.0	34.0	31.0	34.0
4	36.17493240288965	37.0	37.0	37.0	35.0	37.0
5	36.18959928998562	37.0	35.0	37.0	35.0	37.0
6	36.184542830355724	37.0	36.0	37.0	35.0	37.0
7	36.27204818700526	37.0	37.0	37.0	35.0	37.0
8	36.27396244145753	37.0	37.0	37.0	35.0	37.0
9	37.69688107041628	39.0	38.0	39.0	35.0	39.0
10	37.6922792462188	39.0	37.0	39.0	35.0	39.0
11	37.63475698757257	39.0	37.0	39.0	35.0	39.0
12	37.49278891646672	39.0	37.0	39.0	35.0	39.0
13	37.439664004837844	39.0	37.0	39.0	35.0	39.0
14	38.71262472564165	40.0	38.0	41.0	35.0	41.0
15	38.74249253331999	40.0	38.0	41.0	35.0	41.0
16	38.75097398571705	40.0	38.0	41.0	35.0	41.0
17	38.68938611164889	40.0	38.0	41.0	35.0	41.0
18	38.62797878658021	40.0	38.0	41.0	35.0	41.0
19	38.5739794087129	40.0	38.0	41.0	35.0	41.0
20	38.5445172924611	40.0	38.0	41.0	35.0	41.0
21	38.52543565603458	40.0	38.0	41.0	35.0	41.0
22	38.486407705744284	40.0	37.0	41.0	35.0	41.0
23	38.44880892042575	40.0	37.0	41.0	35.0	41.0
24	38.43331977403096	40.0	37.0	41.0	35.0	41.0
25	38.344297153199086	40.0	37.0	41.0	34.0	41.0
26	38.246478750687935	40.0	37.0	41.0	34.0	41.0
27	38.15459888755713	40.0	37.0	41.0	34.0	41.0
28	38.05114322321295	40.0	36.0	41.0	34.0	41.0
29	37.96313755005884	40.0	36.0	41.0	34.0	41.0
30	37.857123745673896	40.0	36.0	41.0	34.0	41.0
31	37.725815679049134	40.0	36.0	41.0	33.0	41.0
32	37.609844488578645	40.0	36.0	41.0	33.0	41.0
33	37.464039207411645	40.0	35.0	41.0	33.0	41.0
34	37.326610964762494	40.0	35.0	41.0	33.0	41.0
35	37.23820286311558	40.0	35.0	41.0	33.0	41.0
36	37.1342262170199	40.0	35.0	41.0	32.0	41.0
37	37.01171393207442	39.0	35.0	41.0	32.0	41.0
38	36.88598221918649	39.0	35.0	41.0	31.0	41.0
39	36.79950577430505	39.0	35.0	41.0	31.0	41.0
40	36.669909660241586	39.0	35.0	41.0	31.0	41.0
41	36.57464178425917	39.0	35.0	41.0	31.0	41.0
42	36.45425475681355	39.0	35.0	41.0	31.0	41.0
43	36.37846985810589	39.0	35.0	41.0	31.0	41.0
44	36.279696503657746	39.0	35.0	41.0	30.0	41.0
45	36.15693949868286	38.0	35.0	40.0	30.0	41.0
46	36.0371278352175	38.0	35.0	40.0	30.0	41.0
47	35.92447396070123	38.0	35.0	40.0	30.0	41.0
48	35.795552186274364	38.0	35.0	40.0	29.0	41.0
49	35.70103369740423	38.0	35.0	40.0	29.0	41.0
50	35.581298169059124	37.0	35.0	40.0	29.0	41.0
51	33.89146721077899	36.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	15.0
9	25.0
10	39.0
11	40.0
12	19.0
13	35.0
14	36.0
15	61.0
16	92.0
17	168.0
18	242.0
19	483.0
20	795.0
21	1234.0
22	1817.0
23	2778.0
24	3964.0
25	5145.0
26	6162.0
27	7404.0
28	8439.0
29	9856.0
30	11690.0
31	14589.0
32	18806.0
33	26835.0
34	55070.0
35	75848.0
36	64325.0
37	98867.0
38	181965.0
39	322415.0
40	158.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.48448475013541	20.493616613988415	25.052261321836205	11.969637314039968
2	31.903552029653543	24.277205797580642	25.671674907387064	18.14756726537875
3	32.429319417283544	24.203463495385126	26.051262864116215	17.315954223215122
4	28.235688228857818	26.15676438790626	25.96958075652206	19.637966626713858
5	25.324389994540024	30.39857823101136	24.125261850431468	20.15176992401715
6	25.954897554757466	35.28993341439911	23.85726622711324	14.897902803730187
7	81.40878251241547	6.272772558292311	7.60687739417762	4.711567535114606
8	82.62901096128202	5.200028713816785	7.301684326389085	4.869275998512102
9	76.49404297066188	7.014872451920671	9.623805494345335	6.867279083072117
10	42.17907415343184	24.62503453271526	16.845547944460517	16.350343369392377
11	31.636317757537917	25.821878623215994	22.263649395595912	20.27815422365018
12	29.198144913412616	21.80966654992615	28.58319067061989	20.408997866041343
13	25.980674731188643	23.294845217300512	29.521175352233698	21.20330469927715
14	21.627921141417723	27.582231368104605	28.367510751366627	22.422336739111046
15	20.81360164799906	24.3960853496451	33.238635745656495	21.551677256699346
16	23.588400488134887	22.70599444431151	30.63905644657816	23.066548620975443
17	22.801707166925166	23.882825874629386	27.579185963294172	25.73628099515128
18	23.855308466877958	25.109471426489367	28.306385126242905	22.728834980389767
19	25.733018061425817	26.94867840307673	25.25434568390003	22.063957851597422
20	27.072017297899325	25.484817569375412	25.610114224433282	21.833050908291984
21	26.278145522493578	26.19222160105632	25.851136262287667	21.678496614162437
22	24.380749561135413	24.91826351017709	25.137097598698304	25.563889329989188
23	23.801905118237844	26.183846737827626	25.667759386916504	24.346488757018026
24	24.78578840092319	23.93079100039373	27.3059696460152	23.977450952667883
25	24.79177044608655	24.91010617586343	25.531586286107082	24.766537091942947
26	23.009773574152344	26.229745338899175	25.939996824077838	24.820484262870643
27	23.84769495485187	25.626755186433158	26.860144134659098	23.665405724055873
28	23.09667637570724	25.96762299628678	27.258657106995948	23.67704352101003
29	24.05369483738626	26.40333341309394	25.599346543139244	23.94362520638056
30	26.213430670271848	24.731188643250405	26.39017291373456	22.665207772743194
31	26.76921704817613	26.843285643744196	23.52238046242297	22.865116845656708
32	26.997839937873742	26.382994459538534	24.17300944728078	22.446156155306944
33	27.007628739050137	25.689185985047065	24.65396587841439	22.64921939748841
34	25.28643119886711	26.06736000382851	25.636652752067068	23.009556045237314
35	24.59784341833638	25.4511005875456	26.150673578285392	23.800382415832626
36	26.430089469642752	27.311081575518426	24.81232692855698	21.446502026281845
37	24.88291506148455	28.64050953973057	25.912370651868898	20.564204746915983
38	25.11719370297297	27.919074893030153	25.141448176998928	21.82228322699795
39	25.346904237245738	26.02972750152814	24.979497899758325	23.643870361467798
40	25.08924123739148	24.611330211068307	28.43353077707854	21.865897774461672
41	22.560358835698235	25.005383840647017	27.56656928622237	24.867688037432377
42	24.101986256523148	25.561061454093785	26.539071456073298	23.79788083330977
43	24.81907032492294	23.85650487591063	27.861429730546934	23.462995068619495
44	24.43545808326572	24.749569836570526	26.60694047756298	24.208031602600776
45	23.82017754710045	24.698450541538232	26.30294381880712	25.1784280925542
46	25.01821804663385	24.90194884154976	27.118024663428386	22.961808448388002
47	23.879889234276465	24.804060829785797	28.355981718869984	22.960068217067754
48	23.694228305297482	24.220974573045122	28.22209267166838	23.862704449989014
49	24.427627042324602	23.907841699857954	28.001953409656977	23.662577848160467
50	22.620505580704314	23.785046627322938	28.50607667024139	25.088371121731356
51	22.699359812403063	23.81647955554492	27.10877968453957	26.37538094751245
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	535.0
1	976.0
2	1417.0
3	2969.5
4	4522.0
5	3275.0
6	2028.0
7	1877.0
8	1726.0
9	1769.5
10	1813.0
11	1950.5
12	2088.0
13	2108.5
14	2129.0
15	2068.0
16	2007.0
17	1915.0
18	1823.0
19	1815.5
20	1808.0
21	2013.0
22	2218.0
23	2756.5
24	3295.0
25	3396.0
26	4856.0
27	6215.0
28	7571.5
29	8928.0
30	10826.5
31	12725.0
32	14059.0
33	15393.0
34	16896.5
35	18400.0
36	20734.0
37	23068.0
38	24434.0
39	25800.0
40	28863.0
41	31926.0
42	37513.5
43	43101.0
44	48012.5
45	52924.0
46	62570.5
47	72217.0
48	78964.5
49	85712.0
50	84358.5
51	83005.0
52	73648.5
53	64292.0
54	60111.5
55	55931.0
56	52284.5
57	48638.0
58	48329.5
59	48021.0
60	47656.5
61	47292.0
62	43907.0
63	40522.0
64	35557.5
65	30593.0
66	26273.5
67	21954.0
68	18875.0
69	15796.0
70	14303.5
71	12811.0
72	10990.5
73	9170.0
74	7834.5
75	4939.5
76	3380.0
77	2660.0
78	1940.0
79	1477.0
80	1014.0
81	740.5
82	467.0
83	379.5
84	292.0
85	261.0
86	230.0
87	177.0
88	124.0
89	93.0
90	62.0
91	44.5
92	27.0
93	28.0
94	29.0
95	21.0
96	13.0
97	7.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	919418.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.52504782463237
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.91847194743394	20.070844276522333
2	10.087952938897883	5.553427741916098
3	3.646867947810911	3.0114064402084275
4	1.970553353130639	2.1695830114364205
5	1.184963062106234	1.6308082477448453
6	0.8449262595820832	1.3953981421970754
7	0.6501285989439781	1.2526374544665966
8	0.5106472713343057	1.124447245199583
9	0.43125863307424867	1.0683373050138851
>10	7.100199360827516	48.18746247904667
>50	0.5821110996697403	9.623862231703113
>100	0.06635669083143053	3.2721174867854663
>500	0.0035761090867237996	0.5977044610362513
>1k	0.001986727270402111	1.0419634767232142
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2518	0.2738689040240674	No Hit
CCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCTGC	2357	0.25635782636406945	RNA PCR Primer, Index 17 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCTGC	1988	0.21622374154084434	RNA PCR Primer, Index 47 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCTGCT	1442	0.15683834773737299	RNA PCR Primer, Index 17 (96% over 25bp)
CGCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCTG	1222	0.13291016708395964	RNA PCR Primer, Index 17 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.2629337254654577E-4	0.0	0.0	0.17206537178954512	0.0
2	4.35057830062061E-4	0.0	0.0	0.8611969746078497	0.0
3	4.35057830062061E-4	0.0	0.0	1.2907078173366195	0.0
4	4.35057830062061E-4	0.0	0.0	1.6676854270853954	0.0
5	4.35057830062061E-4	0.0	0.0	2.6993163066200574	0.0
6	4.35057830062061E-4	0.0	0.0	3.4983000115290324	0.0
7	4.35057830062061E-4	0.0	0.0	4.231372455183605	0.0
8	4.35057830062061E-4	0.0	0.0	5.464326345579486	0.0
9	4.35057830062061E-4	0.0	0.0	6.006408401836814	0.0
10	4.35057830062061E-4	0.0	0.0	7.04206356629955	0.0
11	4.35057830062061E-4	0.0	0.0	8.225203335153326	0.0
12	4.35057830062061E-4	0.0	0.0	9.244870124361281	0.0
13	4.35057830062061E-4	0.0	0.0	9.637836109364837	0.0
14	4.35057830062061E-4	0.0	0.0	9.82741255881438	0.0
15	4.35057830062061E-4	0.0	0.0	10.116943544720682	0.0
16	4.35057830062061E-4	0.0	0.0	10.720695048389308	0.0
17	4.35057830062061E-4	0.0	0.0	11.422660857194442	0.0
18	4.35057830062061E-4	0.0	0.0	12.269392158952728	0.0
19	5.438222875775762E-4	0.0	0.0	12.703144815524604	0.0
20	5.438222875775762E-4	0.0	0.0	13.145816157612751	0.0
21	5.438222875775762E-4	0.0	0.0	13.67354130547803	0.0
22	5.438222875775762E-4	0.0	0.0	14.243793356231878	0.0
23	5.438222875775762E-4	0.0	0.0	14.81023865097268	0.0
24	5.438222875775762E-4	0.0	0.0	15.300440060995108	0.0
25	5.438222875775762E-4	0.0	0.0	15.717116697737048	0.0
26	5.438222875775762E-4	0.0	0.0	16.086807088832284	0.0
27	5.438222875775762E-4	0.0	0.0	16.476727669025404	0.0
28	6.525867450930915E-4	0.0	0.0	16.877850988342626	0.0
29	6.525867450930915E-4	0.0	0.0	17.320848623803318	0.0
30	6.525867450930915E-4	0.0	0.0	17.851836705394064	0.0
31	6.525867450930915E-4	0.0	0.0	18.303426732998485	0.0
32	6.525867450930915E-4	0.0	0.0	18.731849931152098	0.0
33	6.525867450930915E-4	0.0	0.0	19.146351278743726	0.0
34	6.525867450930915E-4	0.0	0.0	19.560417568505294	0.0
35	6.525867450930915E-4	0.0	0.0	20.00232755939083	0.0
36	6.525867450930915E-4	0.0	0.0	20.429880641884324	0.0
37	6.525867450930915E-4	0.0	0.0	20.85145167921446	0.0
38	6.525867450930915E-4	0.0	0.0	21.25833951478	0.0
39	6.525867450930915E-4	0.0	0.0	21.714388885142558	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGTTA	20	7.032551E-4	45.0	16
CCGCTAA	20	7.032551E-4	45.0	40
CTCGAAT	25	3.8901948E-5	45.0	39
TTACGAG	40	6.8121153E-9	45.0	1
TATACGG	55	1.8189894E-12	45.0	2
TGCGTTA	20	7.032551E-4	45.0	13
AATGCGA	95	0.0	40.263157	25
TCGATTG	45	1.927583E-8	40.0	1
AATCGGT	45	1.927583E-8	40.0	26
CGTATGG	85	0.0	39.705883	2
TCGTTGA	40	3.4572076E-7	39.375	24
TCGTTAG	35	6.2469244E-6	38.57143	1
GTATACG	35	6.2469244E-6	38.57143	1
TTACGGG	175	0.0	38.571426	3
TAGCCGT	100	0.0	38.25	44
CGGTCTA	95	0.0	37.894737	31
TCGACGA	30	1.1397441E-4	37.500004	1
TACGTTG	30	1.1397441E-4	37.500004	1
TCACGAC	90	0.0	37.5	25
CTCACGA	90	0.0	37.5	24
>>END_MODULE