FastQCFastQC Report
Sat 18 Jun 2016
SRR3549951_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549951_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences882905
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC35980.4075183626777513No Hit
CCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC31150.35281259025602985No Hit
CTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGCT19070.2159915279673351No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18330.20761010527746473No Hit
CGCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTG13310.15075234594888465No Hit
TCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC12600.14271071066536037No Hit
ACTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC9860.11167679421908358No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGATA207.032396E-445.00000410
TGATTCG406.8121153E-945.00000415
CGTACTC207.032396E-445.00000417
TCGTACT207.032396E-445.00000416
TACTACG207.032396E-445.0000041
CCGGTAT207.032396E-445.00000442
CGGTATC207.032396E-445.00000443
CCATCGA502.1827873E-1145.031
GTTAACG351.2115015E-745.01
ACTACGA351.2115015E-745.032
GCGTAAG950.042.631581
ACTACGG750.042.0000042
TTAACGG650.041.538462
GCGTATG650.041.538461
GACTACG501.0804797E-940.51
TCACGAC451.9274012E-840.025
CGCATGG900.040.02
TAAACGG451.9274012E-840.02
TACGTAG451.9274012E-840.01
ACGGTCT451.9274012E-840.030