##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549951_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	882905
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.45841511827433	33.0	31.0	34.0	31.0	34.0
2	32.6411958251454	34.0	31.0	34.0	31.0	34.0
3	32.6319615360656	34.0	31.0	34.0	31.0	34.0
4	36.08433636687979	37.0	35.0	37.0	35.0	37.0
5	36.09834693426813	37.0	35.0	37.0	35.0	37.0
6	36.088579178960366	37.0	35.0	37.0	35.0	37.0
7	36.20997502562563	37.0	36.0	37.0	35.0	37.0
8	36.211200525537855	37.0	37.0	37.0	35.0	37.0
9	37.644950475985524	39.0	38.0	39.0	35.0	39.0
10	37.57141934862754	39.0	37.0	39.0	35.0	39.0
11	37.553150112413	39.0	37.0	39.0	35.0	39.0
12	37.39755692854837	39.0	37.0	39.0	35.0	39.0
13	37.32482543421999	39.0	37.0	39.0	35.0	39.0
14	38.50637611068009	40.0	38.0	41.0	35.0	41.0
15	38.54668509069492	40.0	38.0	41.0	35.0	41.0
16	38.58235370736376	40.0	38.0	41.0	35.0	41.0
17	38.505212905125695	40.0	38.0	41.0	34.0	41.0
18	38.44663808677038	40.0	37.0	41.0	34.0	41.0
19	38.419059808246644	40.0	37.0	41.0	34.0	41.0
20	38.37420107486083	40.0	37.0	41.0	34.0	41.0
21	38.347932110476215	40.0	37.0	41.0	34.0	41.0
22	38.32762188457422	40.0	37.0	41.0	34.0	41.0
23	38.31263159683092	40.0	37.0	41.0	34.0	41.0
24	38.294324984001676	40.0	37.0	41.0	34.0	41.0
25	38.19746971644741	40.0	37.0	41.0	34.0	41.0
26	38.1397126531167	40.0	37.0	41.0	34.0	41.0
27	38.06838901127528	40.0	36.0	41.0	34.0	41.0
28	37.98113953369842	40.0	36.0	41.0	34.0	41.0
29	37.90859152456946	40.0	36.0	41.0	34.0	41.0
30	37.80484876628856	40.0	36.0	41.0	33.0	41.0
31	37.677130608615876	40.0	36.0	41.0	33.0	41.0
32	37.57475039783442	40.0	36.0	41.0	33.0	41.0
33	37.47735939880282	40.0	35.0	41.0	33.0	41.0
34	37.31569534661147	40.0	35.0	41.0	33.0	41.0
35	37.24490743624739	40.0	35.0	41.0	32.0	41.0
36	37.1373318760229	40.0	35.0	41.0	32.0	41.0
37	37.01936901478641	39.0	35.0	41.0	32.0	41.0
38	36.8952242880038	39.0	35.0	41.0	31.0	41.0
39	36.79180092988487	39.0	35.0	41.0	31.0	41.0
40	36.64363323347359	39.0	35.0	41.0	31.0	41.0
41	36.55963438875077	39.0	35.0	41.0	31.0	41.0
42	36.44246776266982	39.0	35.0	41.0	31.0	41.0
43	36.40458599736098	39.0	35.0	41.0	30.0	41.0
44	36.32751088735481	39.0	35.0	41.0	30.0	41.0
45	36.20545585312123	38.0	35.0	40.0	30.0	41.0
46	36.047167022499586	38.0	35.0	40.0	30.0	41.0
47	35.973018614686744	38.0	35.0	40.0	30.0	41.0
48	35.82355859350666	38.0	35.0	40.0	29.0	41.0
49	35.747275188157275	38.0	35.0	40.0	29.0	41.0
50	35.62938934539956	38.0	35.0	40.0	28.0	41.0
51	33.900976888793245	36.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	16.0
9	27.0
10	31.0
11	30.0
12	14.0
13	16.0
14	20.0
15	31.0
16	70.0
17	104.0
18	230.0
19	357.0
20	688.0
21	1141.0
22	1727.0
23	2514.0
24	3811.0
25	4911.0
26	6213.0
27	7344.0
28	8305.0
29	9780.0
30	11719.0
31	14948.0
32	19461.0
33	27548.0
34	54425.0
35	74206.0
36	61160.0
37	95616.0
38	184387.0
39	291938.0
40	116.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.206233966281765	21.187330460242045	23.566408617008623	12.04002695646757
2	31.979884585544312	24.850012175715396	24.875722756128916	18.29438048261138
3	31.740560989007875	24.675248186384717	26.389475651400772	17.19471517320663
4	27.495823446463664	26.566957939982217	26.124101687044472	19.813116926509647
5	23.588268273483557	30.911479717523406	25.145400694298935	20.354851314694105
6	24.231259308759153	35.122011994495445	25.492776686053425	15.153952010691976
7	80.01438433353532	6.271795946336242	8.606361952871486	5.107457767256953
8	80.45859973609845	5.03429021242376	8.38583992615287	6.121270125324921
9	74.15044653728317	6.828594242868712	11.554470752798998	7.466488467049117
10	39.76056314099479	23.478063891358637	19.787859395971257	16.973513571675323
11	31.500557817658752	25.61328795283751	22.805511351730935	20.080642877772807
12	27.792910902078933	21.457121660880844	29.66899043498451	21.080977002055715
13	25.004955233009213	23.36842582157763	30.915443903930772	20.71117504148238
14	20.700415106948086	27.209948975257813	29.34313431229861	22.746501605495496
15	20.28100418504822	25.25685096358045	34.28409625044597	20.178048600925354
16	22.82533228376779	23.23047213460112	31.28094189069039	22.663253690940703
17	21.879477406969038	24.75498496440727	27.59356895702256	25.771968671601137
18	23.510003907555173	25.90539185982637	28.59209088180495	21.992513350813507
19	24.04448949773758	27.91976486711481	26.293768865279954	21.74197676986765
20	25.079595199936573	26.804242812080574	26.210520950725165	21.905641037257688
21	23.89317083944479	28.087280058443433	27.12375623651469	20.89579286559709
22	21.52825049127596	25.18753433268585	27.067804576936368	26.21641059910183
23	22.637203323120833	26.593008307802084	26.142789994393507	24.626998374683573
24	24.82339549555162	24.30578601321773	27.206098051319223	23.66472043991143
25	22.625197501429938	25.759849587441458	25.05875490568068	26.55619800544792
26	20.1191521171587	26.200214066065996	27.440777886635594	26.23985593013971
27	22.068172679959904	25.93393400195944	26.08276088593903	25.915132432141625
28	19.95990508605116	26.685883532203352	27.086492884285402	26.26771849746009
29	21.781278846535017	24.82339549555162	26.158646740022995	27.236678917890373
30	22.404788737180105	24.82011088395694	25.69982047898698	27.075279899875976
31	22.291186480991726	26.720088797775528	23.36151681098193	27.627207910250817
32	22.96362575815065	26.653037416256563	24.019118704730406	26.364218120862382
33	22.09694134703054	25.911168245734252	24.118336627383467	27.87355377985174
34	19.800205005068495	25.87718950509964	26.72314688443264	27.599458605399224
35	19.51614273336316	26.411335307875706	25.696988917267426	28.375533041493707
36	20.205571380839388	29.046046856683333	23.842315990961655	26.90606577151562
37	20.665303741625657	29.61768253662625	25.803229112984976	23.913784608763116
38	19.990372690153528	29.814079657494297	24.305672750748947	25.889874901603232
39	21.59235704860659	27.00551021910624	25.575798075670654	25.826334656616513
40	21.023666192852005	25.722812760149733	27.626981385313254	25.626539661685005
41	18.857181689989297	27.84908908659482	25.120822738573235	28.172906484842652
42	20.11949190456504	27.090457070692768	25.6574603156625	27.132590709079686
43	21.599492584139856	24.170550625491984	27.10880559063546	27.1211511997327
44	20.775734648688136	23.976871803874708	26.39094806349494	28.85644548394221
45	20.593268811480282	23.351096663853983	27.019781290172784	29.035853234492954
46	21.679455887099973	25.373511306425943	26.226264433885866	26.720768372588218
47	20.22561883781381	25.138944733578356	28.377345240994217	26.25809118761362
48	19.63710705002237	24.581353599764412	27.869363068506804	27.912176281706415
49	20.892734778939975	23.277702584083226	28.926215164711945	26.903347472264855
50	19.628046052519807	22.711390240173067	29.214241622824655	28.44632208448248
51	19.521352806927133	23.327651332816103	26.79484202717167	30.3561538330851
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	688.0
1	898.5
2	1109.0
3	2315.0
4	3521.0
5	2587.5
6	1654.0
7	1543.5
8	1433.0
9	1507.5
10	1582.0
11	1651.5
12	1721.0
13	1764.5
14	1808.0
15	1761.5
16	1715.0
17	1772.0
18	1829.0
19	1860.5
20	1892.0
21	1918.0
22	1944.0
23	2272.0
24	2600.0
25	3188.5
26	4594.0
27	5411.0
28	6183.5
29	6956.0
30	8390.5
31	9825.0
32	11964.0
33	14103.0
34	16016.0
35	17929.0
36	19202.5
37	20476.0
38	22927.0
39	25378.0
40	28514.5
41	31651.0
42	36816.0
43	41981.0
44	48974.5
45	55968.0
46	67768.0
47	79568.0
48	88395.0
49	97222.0
50	96024.5
51	94827.0
52	81936.0
53	69045.0
54	60723.5
55	52402.0
56	49041.5
57	45681.0
58	43950.5
59	42220.0
60	40257.5
61	38295.0
62	34772.5
63	31250.0
64	27412.0
65	23574.0
66	19949.0
67	16324.0
68	14021.5
69	11719.0
70	9898.5
71	8078.0
72	7343.5
73	6609.0
74	5430.5
75	3324.5
76	2397.0
77	1821.5
78	1246.0
79	962.0
80	678.0
81	475.5
82	273.0
83	197.5
84	122.0
85	97.0
86	72.0
87	54.5
88	37.0
89	29.5
90	22.0
91	26.0
92	30.0
93	19.0
94	8.0
95	5.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	882905.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.831748087139903
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.97886502297149	22.192342339865025
2	11.123622135413884	6.859214309912281
3	4.262920326054635	3.942998568253944
4	2.093310205739193	2.581616517263592
5	1.3427296665240058	2.069935141369876
6	0.9418609558177347	1.742353183373112
7	0.67901716241434	1.4654700268882443
8	0.5502010927628593	1.3570929191466858
9	0.47920366876638654	1.3297218118054617
>10	6.091880126548021	43.14817902297715
>50	0.3825998875592813	7.257350469899292
>100	0.06788656947385716	3.877624136476317
>500	0.003689487471405248	0.6923028776870086
>1k	0.002213692482843149	1.4837986750820473
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC	3598	0.4075183626777513	No Hit
CCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC	3115	0.35281259025602985	No Hit
CTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGCT	1907	0.2159915279673351	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1833	0.20761010527746473	No Hit
CGCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTG	1331	0.15075234594888465	No Hit
TCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC	1260	0.14271071066536037	No Hit
ACTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC	986	0.11167679421908358	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.265249375640641E-4	0.0	0.0	0.2460060821945736	0.0
2	2.265249375640641E-4	0.0	0.0	1.3910896415809175	0.0
3	2.265249375640641E-4	0.0	0.0	2.0130138576630556	0.0
4	2.265249375640641E-4	0.0	0.0	2.636070698433014	0.0
5	2.265249375640641E-4	0.0	0.0	4.408401809934251	0.0
6	2.265249375640641E-4	0.0	0.0	5.667880462790447	0.0
7	2.265249375640641E-4	0.0	0.0	6.7898584785452565	0.0
8	2.265249375640641E-4	0.0	0.0	8.753603162288128	0.0
9	2.265249375640641E-4	0.0	0.0	9.596615717432792	0.0
10	2.265249375640641E-4	0.0	0.0	11.104252439390422	0.0
11	2.265249375640641E-4	0.0	0.0	12.8599339679807	0.0
12	2.265249375640641E-4	0.0	0.0	14.286927812165521	0.0
13	2.265249375640641E-4	0.0	0.0	14.855958455326451	0.0
14	2.265249375640641E-4	0.0	0.0	15.090185240767694	0.0
15	2.265249375640641E-4	0.0	0.0	15.507330913291916	0.0
16	2.265249375640641E-4	0.0	0.0	16.401764629263624	0.0
17	2.265249375640641E-4	0.0	0.0	17.430187845804475	0.0
18	9.060997502562564E-4	0.0	0.0	18.628391503049592	0.0
19	9.060997502562564E-4	0.0	0.0	19.23740379769058	0.0
20	0.0010193622190382884	0.0	0.0	19.8449436802374	0.0
21	0.0010193622190382884	0.0	0.0	20.602329808982848	0.0
22	0.0010193622190382884	0.0	0.0	21.44024555303232	0.0
23	0.0010193622190382884	0.0	0.0	22.253809866293654	0.0
24	0.0010193622190382884	0.0	0.0	22.895441751943867	0.0
25	0.0010193622190382884	0.0	0.0	23.478290416296204	0.0
26	0.0010193622190382884	0.0	0.0	24.021723741512393	0.0
27	0.0010193622190382884	0.0	0.0	24.567535578573008	0.0
28	0.0010193622190382884	0.0	0.0	25.091714284096252	0.0
29	0.0010193622190382884	0.0	0.0	25.67931997213743	0.0
30	0.0010193622190382884	0.0	0.0	26.35187251176514	0.0
31	0.0010193622190382884	0.0	0.0	26.937326212899464	0.0
32	0.0011326246878203205	0.0	0.0	27.501599832371546	0.0
33	0.0011326246878203205	0.0	0.0	28.05035649362049	0.0
34	0.0012458871566023524	0.0	0.0	28.55969781573329	0.0
35	0.0012458871566023524	0.0	0.0	29.08421630866288	0.0
36	0.0012458871566023524	0.0	0.0	29.609640901342726	0.0
37	0.0012458871566023524	0.0	0.0	30.133479819459623	0.0
38	0.0012458871566023524	0.0	0.0	30.69888606361953	0.0
39	0.0012458871566023524	0.0	0.0	31.284339764753852	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGATA	20	7.032396E-4	45.000004	10
TGATTCG	40	6.8121153E-9	45.000004	15
CGTACTC	20	7.032396E-4	45.000004	17
TCGTACT	20	7.032396E-4	45.000004	16
TACTACG	20	7.032396E-4	45.000004	1
CCGGTAT	20	7.032396E-4	45.000004	42
CGGTATC	20	7.032396E-4	45.000004	43
CCATCGA	50	2.1827873E-11	45.0	31
GTTAACG	35	1.2115015E-7	45.0	1
ACTACGA	35	1.2115015E-7	45.0	32
GCGTAAG	95	0.0	42.63158	1
ACTACGG	75	0.0	42.000004	2
TTAACGG	65	0.0	41.53846	2
GCGTATG	65	0.0	41.53846	1
GACTACG	50	1.0804797E-9	40.5	1
TCACGAC	45	1.9274012E-8	40.0	25
CGCATGG	90	0.0	40.0	2
TAAACGG	45	1.9274012E-8	40.0	2
TACGTAG	45	1.9274012E-8	40.0	1
ACGGTCT	45	1.9274012E-8	40.0	30
>>END_MODULE