FastQCFastQC Report
Sat 18 Jun 2016
SRR3549945_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549945_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1601904
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC59810.37336819185169645No Hit
CCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC45420.2835375902675816No Hit
CTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCT30010.1873395659165593No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24230.1512575035707508No Hit
TCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC20870.13028246386799708No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCGT302.1660398E-645.00000426
TCGACGA253.891496E-545.014
CGATATT207.0341205E-445.012
CGGTCTA1150.043.0434831
TATAGCG556.184564E-1140.9090921
CTAACGG950.040.2631572
CGCTTAA451.9290383E-840.029
TCACGAC1250.039.60000225
AATTGCG403.459354E-739.3751
TAACGCG356.2498275E-638.571431
TTGAGCG1400.038.571431
ACGCTAG356.2498275E-638.571431
CGGATGG1350.038.3333362
ATGCGAA2000.038.2526
TAAGGGA12100.038.1198354
GCGTACG1250.037.8000031
TTGCGAG1550.037.7419361
CGATCTA301.1401224E-437.50000410
TACGAAA2100.037.520
CGACAGG3550.037.3943672
Produced by FastQC (version 0.11.5)