##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549945_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1601904
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.55670252399644	34.0	31.0	34.0	31.0	34.0
2	32.73480558135818	34.0	31.0	34.0	31.0	34.0
3	32.737067889211836	34.0	31.0	34.0	31.0	34.0
4	36.16386187936356	37.0	37.0	37.0	35.0	37.0
5	36.17273382175212	37.0	35.0	37.0	35.0	37.0
6	36.16681024580749	37.0	36.0	37.0	35.0	37.0
7	36.288920559534155	37.0	37.0	37.0	35.0	37.0
8	36.277329353069845	37.0	37.0	37.0	35.0	37.0
9	37.68381001608086	39.0	38.0	39.0	35.0	39.0
10	37.65109831800158	39.0	37.0	39.0	35.0	39.0
11	37.62442505917958	39.0	37.0	39.0	35.0	39.0
12	37.5008864451303	39.0	37.0	39.0	35.0	39.0
13	37.4538836284821	39.0	37.0	39.0	35.0	39.0
14	38.661948531247816	40.0	38.0	41.0	35.0	41.0
15	38.7075654970585	40.0	38.0	41.0	35.0	41.0
16	38.73550412509114	40.0	38.0	41.0	35.0	41.0
17	38.702636362728356	40.0	38.0	41.0	35.0	41.0
18	38.65407103047374	40.0	38.0	41.0	35.0	41.0
19	38.63208781549955	40.0	38.0	41.0	35.0	41.0
20	38.59802709775367	40.0	38.0	41.0	35.0	41.0
21	38.568457910087	40.0	38.0	41.0	35.0	41.0
22	38.549838192550865	40.0	38.0	41.0	35.0	41.0
23	38.51533674926837	40.0	38.0	41.0	35.0	41.0
24	38.487695891888656	40.0	38.0	41.0	35.0	41.0
25	38.409080069717035	40.0	37.0	41.0	34.0	41.0
26	38.323210379648216	40.0	37.0	41.0	34.0	41.0
27	38.255084574356516	40.0	37.0	41.0	34.0	41.0
28	38.16695382494831	40.0	37.0	41.0	34.0	41.0
29	38.10773679321607	40.0	37.0	41.0	34.0	41.0
30	37.99435609125141	40.0	37.0	41.0	34.0	41.0
31	37.88173635873311	40.0	36.0	41.0	34.0	41.0
32	37.80917707927566	40.0	36.0	41.0	34.0	41.0
33	37.716258901906734	40.0	36.0	41.0	33.0	41.0
34	37.59886797211318	40.0	36.0	41.0	33.0	41.0
35	37.54914339436071	40.0	36.0	41.0	33.0	41.0
36	37.45008315105025	40.0	36.0	41.0	33.0	41.0
37	37.34956089753194	40.0	35.0	41.0	33.0	41.0
38	37.22768093468772	40.0	35.0	41.0	32.0	41.0
39	37.13177443841828	39.0	35.0	41.0	32.0	41.0
40	37.009157227898804	39.0	35.0	41.0	32.0	41.0
41	36.94956252060049	39.0	35.0	41.0	32.0	41.0
42	36.84809202049561	39.0	35.0	41.0	31.0	41.0
43	36.81225279417493	39.0	35.0	41.0	31.0	41.0
44	36.72387359042739	39.0	35.0	41.0	31.0	41.0
45	36.59129386030624	39.0	35.0	41.0	31.0	41.0
46	36.47203328039633	39.0	35.0	41.0	31.0	41.0
47	36.40401921713161	39.0	35.0	40.0	31.0	41.0
48	36.300813906451324	38.0	35.0	40.0	31.0	41.0
49	36.22088964132682	38.0	35.0	40.0	30.0	41.0
50	36.12420719343981	38.0	35.0	40.0	30.0	41.0
51	34.46351529180276	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	27.0
9	49.0
10	66.0
11	62.0
12	35.0
13	40.0
14	45.0
15	71.0
16	109.0
17	201.0
18	330.0
19	662.0
20	1083.0
21	1682.0
22	2650.0
23	3945.0
24	5593.0
25	7539.0
26	9264.0
27	10866.0
28	12902.0
29	15289.0
30	18682.0
31	23816.0
32	31099.0
33	45324.0
34	90412.0
35	124624.0
36	107355.0
37	170844.0
38	333841.0
39	583086.0
40	308.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.00550844495051	21.096020735324963	24.53979764080744	10.358673178917089
2	32.51593104205995	24.302205375603034	24.91522588120137	18.26663770113565
3	32.89142170816728	24.130534663750137	25.400023971473946	17.578019656608635
4	29.775567075180536	26.283222964672042	24.130534663750137	19.81067529639729
5	24.624883888173073	31.058914891279375	23.341598497787633	20.974602722759915
6	25.527185149671887	35.97743685014832	23.099636432645152	15.395741567534635
7	82.52716767047214	5.700154316363528	7.179456446828274	4.5932215663360605
8	82.33202489038045	5.1094197904493655	7.290449365255346	5.268105953914842
9	75.46394790199662	7.69153457385711	9.866945834456995	6.97757168968927
10	38.28412938603062	28.127028835685536	17.25683936116022	16.332002417123622
11	30.910528970525075	25.51657277839371	23.267499175980582	20.30539907510063
12	27.443155145377002	22.58418731709266	29.734990361469848	20.23766717606049
13	25.83850218240294	23.793747939951455	30.70308832489338	19.664661552752225
14	21.291975049690866	28.26305446518643	28.649532056852344	21.79543842827036
15	20.37138305416554	25.693612101599093	33.10984927935757	20.825155564877797
16	22.818720722340416	24.57837673168929	30.30281465056583	22.30008789540447
17	22.75560832609195	24.24502342212767	27.275916659175582	25.7234515926048
18	23.658845973291783	25.22385860825618	29.080644033600016	22.03665138485203
19	23.93632826935946	27.16567284930932	26.439661802455078	22.458337078876138
20	24.954678932070838	26.339905512440197	26.266742576334163	22.438672979154806
21	24.174856920264883	26.747795123802675	27.526805601334413	21.55054235459803
22	22.12510862074132	25.241212956581666	25.983704391773788	26.649974030903223
23	21.849623947502472	26.64803883378779	27.084893976168363	24.417443242541374
24	22.937891409223024	25.645044896573076	28.010854583046175	23.40620911115772
25	22.897626824079346	26.76764650066421	25.69904313866499	24.635683536591458
26	20.968547428560015	28.07939801636053	26.392592814550685	24.55946174052877
27	22.465141481636852	26.49060118459034	27.257313796582068	23.786943537190744
28	20.25658216722101	27.207997482995232	27.755720692376073	24.779699657407686
29	21.138282943297476	27.02671321127858	26.03882629670692	25.796177548717026
30	22.62850957360741	27.540601683996048	25.76003306065782	24.07085568173873
31	23.321622269499294	29.20118808617745	23.03196695931841	24.445222685004843
32	22.457026138894715	28.75421997822591	23.385046794314764	25.40370708856461
33	21.918229806530228	27.6919840389936	24.211126259750895	26.178659894725275
34	20.069804432725057	28.868022053756032	25.54204246946134	25.52013104405757
35	20.078044626893995	27.302447587371027	25.282476353139767	27.33703143259521
36	22.284481467054206	29.230278468622338	24.93582636662372	23.549413697699737
37	20.707545520830212	29.47298964232563	25.615330256994177	24.204134579849978
38	20.440862873180915	29.876821582317042	24.502904044187417	25.179411500314625
39	21.925908169278557	27.2116806000859	26.344587440945276	24.51782378969027
40	22.071047952936006	26.64173383673429	27.5971593803374	23.690058829992306
41	19.321444980473238	26.875892687701636	26.1917068688261	27.61095546299903
42	20.431499016170758	27.029896922662033	26.735996663969875	25.802607397197335
43	21.532875877705532	24.667707927566195	27.722572638560113	26.07684355616816
44	21.243095716097844	26.24289595381496	26.304385281514996	26.2096230485722
45	21.725396777834376	25.809599077098255	25.251638050719645	27.213366094347723
46	22.402341213955392	27.080898730510693	25.790933788791335	24.725826266742576
47	20.448478810215843	26.33559813821552	28.164234560872565	25.051688490696073
48	19.76922462269899	25.682125770333304	27.95123802674817	26.59741158021954
49	20.763541385750955	25.177413877485794	29.278096565087548	24.780948171675707
50	20.242099401711965	24.800487419970235	28.39065262337818	26.566760554939624
51	20.25845493862304	24.821774588239993	26.14619852375673	28.77357194938024
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	689.0
1	1375.0
2	2061.0
3	3332.5
4	4604.0
5	3455.0
6	2306.0
7	2153.0
8	2000.0
9	2074.5
10	2149.0
11	2317.5
12	2486.0
13	2499.5
14	2513.0
15	2553.5
16	2594.0
17	2753.0
18	2912.0
19	2997.0
20	3082.0
21	3407.5
22	3733.0
23	4173.0
24	4613.0
25	5758.5
26	8422.0
27	9940.0
28	12782.5
29	15625.0
30	17840.5
31	20056.0
32	22539.0
33	25022.0
34	30109.0
35	35196.0
36	38501.5
37	41807.0
38	46925.0
39	52043.0
40	60344.5
41	68646.0
42	81296.5
43	93947.0
44	113100.5
45	132254.0
46	152588.5
47	172923.0
48	173784.5
49	174646.0
50	153853.0
51	133060.0
52	117572.5
53	102085.0
54	92967.0
55	83849.0
56	79539.5
57	75230.0
58	73079.5
59	70929.0
60	67147.5
61	63366.0
62	58811.0
63	54256.0
64	47337.5
65	40419.0
66	35641.0
67	30863.0
68	25723.5
69	20584.0
70	18260.0
71	15936.0
72	13164.5
73	10393.0
74	8595.0
75	5387.0
76	3977.0
77	3303.5
78	2630.0
79	1906.5
80	1183.0
81	1097.5
82	1012.0
83	670.5
84	329.0
85	229.0
86	129.0
87	91.0
88	53.0
89	42.5
90	32.0
91	28.5
92	25.0
93	18.0
94	11.0
95	7.0
96	3.0
97	2.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1601904.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.828585215538126
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.15316857779901	18.119570927516936
2	12.05578461977093	6.227677207838547
3	4.784748706894967	3.7074986913291754
4	2.5201556676439765	2.603682216726553
5	1.4452190950874204	1.866398227629417
6	0.9507709139225174	1.4734240542421682
7	0.7124150266813378	1.288047055782816
8	0.5395796428192018	1.1149263028100302
9	0.439126505021407	1.0207814719812156
>10	4.875727996682606	30.82913554067958
>50	1.4034406173684706	24.05445865409968
>100	0.11133581748619635	4.937994910726773
>500	0.0048724644697300075	0.8577858554297599
>1k	0.0034107251288110047	1.522267856114785
>5k	2.4362322348650035E-4	0.3763510270925319
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC	5981	0.37336819185169645	No Hit
CCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC	4542	0.2835375902675816	No Hit
CTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCT	3001	0.1873395659165593	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2423	0.1512575035707508	No Hit
TCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC	2087	0.13028246386799708	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.242571340105275E-5	0.0	0.0	0.201198074291593	0.0
2	6.242571340105275E-5	0.0	0.0	1.1554999550534863	0.0
3	6.242571340105275E-5	0.0	0.0	1.6470400223733757	0.0
4	6.242571340105275E-5	0.0	0.0	2.173788192051459	0.0
5	6.242571340105275E-5	0.0	0.0	3.636922062745333	0.0
6	6.242571340105275E-5	0.0	0.0	4.521681698778454	0.0
7	6.242571340105275E-5	0.0	0.0	5.347698738501183	0.0
8	6.242571340105275E-5	0.0	0.0	6.768820104076149	0.0
9	6.242571340105275E-5	0.0	0.0	7.301810845094337	0.0
10	6.242571340105275E-5	0.0	0.0	8.475788811314535	0.0
11	6.242571340105275E-5	0.0	0.0	9.993357904094127	0.0
12	6.242571340105275E-5	0.0	0.0	11.174826955922452	0.0
13	1.248514268021055E-4	0.0	0.0	11.655255246256955	0.0
14	1.248514268021055E-4	0.0	0.0	11.843780900728133	0.0
15	1.248514268021055E-4	0.0	0.0	12.215838152598408	0.0
16	1.248514268021055E-4	0.0	0.0	13.033365295298594	0.0
17	1.248514268021055E-4	0.0	0.0	14.0180060727734	0.0
18	1.248514268021055E-4	0.0	0.0	15.108083880182583	0.0
19	1.248514268021055E-4	0.0	0.0	15.683024700606278	0.0
20	1.8727714020315824E-4	0.0	0.0	16.275944126489478	0.0
21	1.8727714020315824E-4	0.0	0.0	17.02218110448566	0.0
22	1.8727714020315824E-4	0.0	0.0	17.827098253078837	0.0
23	1.8727714020315824E-4	0.0	0.0	18.63026748169678	0.0
24	1.8727714020315824E-4	0.0	0.0	19.281804652463567	0.0
25	1.8727714020315824E-4	0.0	0.0	19.8470694873101	0.0
26	1.8727714020315824E-4	0.0	0.0	20.378125031212857	0.0
27	2.49702853604211E-4	0.0	0.0	20.913363097913482	0.0
28	2.49702853604211E-4	0.0	0.0	21.47712971563839	0.0
29	3.1212856700526376E-4	0.0	0.0	22.086217401292462	0.0
30	3.1212856700526376E-4	0.0	0.0	22.78394959997603	0.0
31	3.1212856700526376E-4	0.0	0.0	23.370376751665518	0.0
32	3.1212856700526376E-4	0.0	0.0	23.949500094887085	0.0
33	3.1212856700526376E-4	0.0	0.0	24.493914741457665	0.0
34	3.1212856700526376E-4	0.0	0.0	25.059366853444402	0.0
35	3.1212856700526376E-4	0.0	0.0	25.67419770473137	0.0
36	3.1212856700526376E-4	0.0	0.0	26.19901067729402	0.0
37	3.1212856700526376E-4	0.0	0.0	26.74810725236968	0.0
38	3.1212856700526376E-4	0.0	0.0	27.286591456167162	0.0
39	3.1212856700526376E-4	0.0	0.0	27.877450833508124	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGCGT	30	2.1660398E-6	45.000004	26
TCGACGA	25	3.891496E-5	45.0	14
CGATATT	20	7.0341205E-4	45.0	12
CGGTCTA	115	0.0	43.04348	31
TATAGCG	55	6.184564E-11	40.909092	1
CTAACGG	95	0.0	40.263157	2
CGCTTAA	45	1.9290383E-8	40.0	29
TCACGAC	125	0.0	39.600002	25
AATTGCG	40	3.459354E-7	39.375	1
TAACGCG	35	6.2498275E-6	38.57143	1
TTGAGCG	140	0.0	38.57143	1
ACGCTAG	35	6.2498275E-6	38.57143	1
CGGATGG	135	0.0	38.333336	2
ATGCGAA	200	0.0	38.25	26
TAAGGGA	1210	0.0	38.119835	4
GCGTACG	125	0.0	37.800003	1
TTGCGAG	155	0.0	37.741936	1
CGATCTA	30	1.1401224E-4	37.500004	10
TACGAAA	210	0.0	37.5	20
CGACAGG	355	0.0	37.394367	2
>>END_MODULE