FastQCFastQC Report
Sat 18 Jun 2016
SRR3549944_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549944_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1974299
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGC47160.23886959371402205No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42480.21516497754392824No Hit
CCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGC36410.1844198877677596No Hit
CTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGCT23590.11948544774626337No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT22660.11477491504579601No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCGA253.8918275E-545.042
GTGCGAT207.0345186E-445.026
ATATGCG700.041.7857131
AATGCGA1300.039.80769325
TCTAGCG301.1402185E-437.5000041
CGACGGT1800.037.528
AATACGG900.037.52
TCTACGG1400.036.9642832
GCGTAAG1300.036.3461571
CGGGTAT2050.036.2195136
TGATTCG2050.036.21951315
CGCATCG751.8189894E-1236.021
TAAATCG250.002107474536.039
ATGCGAA1450.035.68965526
GTTAACG702.1827873E-1135.3571431
CACGACG1850.035.2702726
TTAGGCG1150.035.2173921
CGGTCTA2000.034.87531
TAGGGAC12650.034.6837925
TGTTACG653.6743586E-1034.6153871
Produced by FastQC (version 0.11.5)