##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549944_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1974299
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.60639649820012	34.0	31.0	34.0	31.0	34.0
2	32.78697198347363	34.0	31.0	34.0	31.0	34.0
3	32.78363814194304	34.0	31.0	34.0	31.0	34.0
4	36.20309841619734	37.0	37.0	37.0	35.0	37.0
5	36.20623623878652	37.0	37.0	37.0	35.0	37.0
6	36.19968353324395	37.0	37.0	37.0	35.0	37.0
7	36.29569989145514	37.0	37.0	37.0	35.0	37.0
8	36.28010904123438	37.0	37.0	37.0	35.0	37.0
9	37.69803915212437	39.0	38.0	39.0	35.0	39.0
10	37.68153709240596	39.0	37.0	39.0	35.0	39.0
11	37.635070979623656	39.0	37.0	39.0	35.0	39.0
12	37.52670340206828	39.0	37.0	39.0	35.0	39.0
13	37.50352150307527	39.0	37.0	39.0	35.0	39.0
14	38.775920465947664	40.0	38.0	41.0	35.0	41.0
15	38.796739500957045	40.0	38.0	41.0	35.0	41.0
16	38.804392343814186	40.0	38.0	41.0	35.0	41.0
17	38.74902788280802	40.0	38.0	41.0	35.0	41.0
18	38.70152038774269	40.0	38.0	41.0	35.0	41.0
19	38.67160090746133	40.0	38.0	41.0	35.0	41.0
20	38.63521533465802	40.0	38.0	41.0	35.0	41.0
21	38.61563724643532	40.0	38.0	41.0	35.0	41.0
22	38.57598165222188	40.0	38.0	41.0	35.0	41.0
23	38.53953023326254	40.0	38.0	41.0	35.0	41.0
24	38.5228134137737	40.0	38.0	41.0	35.0	41.0
25	38.43829328789611	40.0	37.0	41.0	35.0	41.0
26	38.351549587980344	40.0	37.0	41.0	34.0	41.0
27	38.25600529605698	40.0	37.0	41.0	34.0	41.0
28	38.16832658072561	40.0	37.0	41.0	34.0	41.0
29	38.09434589188365	40.0	37.0	41.0	34.0	41.0
30	37.992124293230155	40.0	37.0	41.0	34.0	41.0
31	37.88668990867138	40.0	36.0	41.0	34.0	41.0
32	37.79869513179108	40.0	36.0	41.0	33.0	41.0
33	37.68894529146801	40.0	36.0	41.0	33.0	41.0
34	37.550297092790906	40.0	36.0	41.0	33.0	41.0
35	37.491526865991425	40.0	36.0	41.0	33.0	41.0
36	37.37032992469732	40.0	35.0	41.0	33.0	41.0
37	37.27674227662578	40.0	35.0	41.0	32.0	41.0
38	37.16483116285831	40.0	35.0	41.0	32.0	41.0
39	37.059516314398174	39.0	35.0	41.0	32.0	41.0
40	36.913430032634366	39.0	35.0	41.0	31.0	41.0
41	36.81710267796316	39.0	35.0	41.0	31.0	41.0
42	36.69734320890605	39.0	35.0	41.0	31.0	41.0
43	36.67063702103886	39.0	35.0	41.0	31.0	41.0
44	36.594469733307875	39.0	35.0	41.0	31.0	41.0
45	36.45321352034317	39.0	35.0	41.0	31.0	41.0
46	36.310925548764395	38.0	35.0	40.0	31.0	41.0
47	36.21016877382808	38.0	35.0	40.0	30.0	41.0
48	36.101226308679685	38.0	35.0	40.0	30.0	41.0
49	36.014712563801126	38.0	35.0	40.0	30.0	41.0
50	35.906570889211814	38.0	35.0	40.0	30.0	41.0
51	34.250152585803875	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	39.0
9	74.0
10	81.0
11	76.0
12	47.0
13	42.0
14	64.0
15	79.0
16	171.0
17	280.0
18	482.0
19	828.0
20	1357.0
21	2125.0
22	3220.0
23	4909.0
24	6903.0
25	9336.0
26	12107.0
27	14355.0
28	16589.0
29	19296.0
30	23687.0
31	30138.0
32	39121.0
33	56115.0
34	113181.0
35	146523.0
36	134929.0
37	215733.0
38	411392.0
39	710684.0
40	332.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.73734728123755	20.039163267569908	23.863406707899866	11.360082743292683
2	32.605902145521014	23.93497641441342	25.05248698398773	18.40663445607783
3	33.08394523828457	23.920338307419495	25.6671861759541	17.32853027834183
4	29.414338962842002	25.55702049183026	25.223889593217642	19.80475095211009
5	25.36490166889615	30.402689764822853	23.62443581240734	20.607972753873653
6	26.186408441679802	35.72614887613275	23.128107748623687	14.959334933563762
7	82.85330641407405	5.686322081913631	7.048982955469257	4.411388548543052
8	83.1589845307119	4.88659519150848	7.131088046947297	4.82333223083231
9	76.23830027771884	7.089959524874398	9.675940675652473	6.995799521754304
10	39.42467680933841	26.639429995152707	17.04782305010538	16.888070145403507
11	30.357205266274256	24.83661289399427	23.472888351764347	21.333293487967122
12	26.976612964905517	21.790417763469463	29.89911862387612	21.333850647748896
13	24.87556342782932	22.85854371602275	31.2597028109724	21.006190045175526
14	22.06403386721059	26.143861694707844	29.234578956885453	22.557525481196112
15	21.04027809364235	24.988261656415773	32.3442903025327	21.62716994740918
16	24.182963168192863	23.223432722196588	29.781102051918175	22.812502057692377
17	23.57500054449706	23.518524802980703	27.092096992400847	25.81437766012139
18	24.14998943928959	24.353099505191462	28.586754083348065	22.91015697217088
19	24.684660226237263	25.73227256864335	26.12734950481158	23.455717700307805
20	25.588778599391482	24.816504490961094	26.491124191421868	23.103592718225556
21	25.44275208567699	25.29252154815456	27.266133447871876	21.99859291829657
22	23.64651960012136	23.1055681029064	27.05081651766019	26.19709577931205
23	22.9939841938835	25.331573383768113	27.049702198096643	24.62474022425175
24	24.246124827090526	24.190763405137723	27.62428588577515	23.938825881996596
25	24.579964838152684	25.03830473499708	25.61759895537606	24.76413147147418
26	22.43429186764517	25.571658598824193	25.864825945816715	26.129223587713916
27	22.423098021120406	24.71130259398399	27.628895116697116	25.236704268198483
28	21.526222725129273	25.335929360243814	27.98557867881207	25.15226923581484
29	22.69053471637275	25.95174287177373	25.924340740688212	25.43338167116531
30	24.347679860041463	24.253317253364358	26.682179345681682	24.716823540912497
31	24.61851016487371	25.473699779010172	24.600427797410624	25.307362258705496
32	23.532859004639114	27.166351196044776	24.256964117390527	25.043825681925586
33	24.462100218862492	25.02462899489895	24.25397571492464	26.25929507131392
34	22.07963434110031	25.149837993130724	26.929001129008324	25.841526536760647
35	22.34919837370125	25.60883635153541	25.781403931218115	26.26056134354523
36	23.372194383930704	28.029391698015342	24.66419726697932	23.934216651074635
37	21.908586288095165	27.364852031024682	26.09174192966719	24.634819751212962
38	22.5204490302634	28.082372528173288	24.615775016854084	24.781403424709225
39	23.257520770663408	25.559502385403633	25.992618139400363	25.190358704532596
40	23.351174264890982	25.012422130589133	28.24445537378077	23.39194823073911
41	20.708464118150292	26.538026914869533	26.838538640803648	25.914970326176533
42	21.050762827717584	27.526782923964404	25.717431858092414	25.705022390225597
43	22.540101575293306	24.28933003562277	26.448628095339156	26.721940293744765
44	22.856669633120415	23.689066347093323	26.446247503544296	27.008016516241966
45	22.921553422252657	23.576216165839114	26.49907638103448	27.003154030873745
46	23.720014040426502	24.963999880463902	26.628692006631216	24.687294072478384
47	21.34514579605217	25.90468819565831	28.587665799354607	24.16250020893492
48	21.133982238759174	25.737033752233067	27.543649670085436	25.585334338922323
49	21.838789362705448	24.755976678304553	28.169593359465818	25.235640599524185
50	21.53073065427273	23.545015218059675	29.077459898424706	25.846794229242885
51	21.924490667320402	23.662474630235845	27.09589580909477	27.31713889334898
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	647.0
1	1835.5
2	3024.0
3	5447.0
4	7870.0
5	5691.0
6	3512.0
7	3130.5
8	2749.0
9	2840.5
10	2932.0
11	2855.5
12	2779.0
13	2959.5
14	3140.0
15	3163.5
16	3187.0
17	3125.0
18	3063.0
19	3406.0
20	3749.0
21	4031.0
22	4313.0
23	4961.5
24	5610.0
25	6834.5
26	10559.5
27	13060.0
28	16388.5
29	19717.0
30	22833.5
31	25950.0
32	29424.0
33	32898.0
34	37193.0
35	41488.0
36	44734.0
37	47980.0
38	52822.5
39	57665.0
40	66179.5
41	74694.0
42	82039.0
43	89384.0
44	101076.0
45	112768.0
46	133679.0
47	154590.0
48	170619.5
49	186649.0
50	184515.0
51	182381.0
52	166563.0
53	150745.0
54	137224.0
55	123703.0
56	118113.0
57	112523.0
58	108234.5
59	103946.0
60	100724.5
61	97503.0
62	89962.5
63	82422.0
64	74239.5
65	66057.0
66	56822.0
67	47587.0
68	39539.0
69	31491.0
70	27524.5
71	23558.0
72	19499.5
73	15441.0
74	13021.0
75	8906.5
76	7212.0
77	5495.0
78	3778.0
79	2788.5
80	1799.0
81	1492.5
82	1186.0
83	828.5
84	471.0
85	340.0
86	209.0
87	138.5
88	68.0
89	61.5
90	55.0
91	50.5
92	46.0
93	38.5
94	31.0
95	18.0
96	5.0
97	4.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1974299.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.104048515544026
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.32687796067128	17.19266526826308
2	10.898602518262786	5.254008877036729
3	4.116285995723748	2.9765747203433905
4	2.015651906018968	1.9434148533251996
5	1.242544650210025	1.4975178265696063
6	0.863580378829479	1.248947000902659
7	0.6174266203231814	1.0417736847740842
8	0.47649408068911125	0.9188349150639911
9	0.40778017681216866	0.8846237849001872
>10	6.729739953383939	42.54645435145869
>50	1.2061973891076616	17.838634880004037
>100	0.08821555168651894	3.8099506376393943
>500	0.006785803699968233	1.0738575817797658
>1k	0.003817014581232131	1.7727416179392563
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGC	4716	0.23886959371402205	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4248	0.21516497754392824	No Hit
CCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGC	3641	0.1844198877677596	No Hit
CTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGCT	2359	0.11948544774626337	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	2266	0.11477491504579601	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.0390533551402296E-4	0.0	0.0	0.12991953093224481	0.0
2	3.0390533551402296E-4	0.0	0.0	0.7389458233023468	0.0
3	3.0390533551402296E-4	0.0	0.0	1.0758755386088936	0.0
4	3.0390533551402296E-4	0.0	0.0	1.4466400479360015	0.0
5	3.0390533551402296E-4	0.0	0.0	2.5112710891308763	0.0
6	3.0390533551402296E-4	0.0	0.0	3.2035674434318206	0.0
7	3.0390533551402296E-4	0.0	0.0	3.8305748014865024	0.0
8	3.0390533551402296E-4	0.0	0.0	4.825560869959413	0.0
9	3.0390533551402296E-4	0.0	0.0	5.229248457300541	0.0
10	3.0390533551402296E-4	0.0	0.0	6.119336534131861	0.0
11	3.0390533551402296E-4	0.0	0.0	7.182346746870661	0.0
12	3.0390533551402296E-4	0.0	0.0	8.113968552888899	0.0
13	3.0390533551402296E-4	0.0	0.0	8.46356099050853	0.0
14	3.0390533551402296E-4	0.0	0.0	8.619211173180961	0.0
15	3.0390533551402296E-4	0.0	0.0	8.903717218111339	0.0
16	3.545562247663601E-4	0.0	0.0	9.493546823454807	0.0
17	3.545562247663601E-4	0.0	0.0	10.1890848346679	0.0
18	3.545562247663601E-4	0.0	0.0	10.97493338141791	0.0
19	3.545562247663601E-4	0.0	0.0	11.412810319004366	0.0
20	3.545562247663601E-4	0.0	0.0	11.861779801337082	0.0
21	3.545562247663601E-4	0.0	0.0	12.397109049845033	0.0
22	4.0520711401869725E-4	0.0	0.0	12.96981865462121	0.0
23	4.0520711401869725E-4	0.0	0.0	13.551999975687574	0.0
24	4.0520711401869725E-4	0.0	0.0	14.023002594845057	0.0
25	4.0520711401869725E-4	0.0	0.0	14.461031485099268	0.0
26	4.0520711401869725E-4	0.0	0.0	14.862135877088527	0.0
27	4.0520711401869725E-4	0.0	0.0	15.283399323000214	0.0
28	4.0520711401869725E-4	0.0	0.0	15.727911527078724	0.0
29	4.0520711401869725E-4	0.0	0.0	16.177995328974994	0.0
30	4.0520711401869725E-4	0.0	0.0	16.71838966640818	0.0
31	4.0520711401869725E-4	0.0	0.0	17.150897609733885	0.0
32	4.0520711401869725E-4	0.0	0.0	17.611668749262396	0.0
33	4.5585800327103444E-4	0.0	0.0	18.056738113122684	0.0
34	5.065088925233716E-4	0.0	0.0	18.497400849618018	0.0
35	5.065088925233716E-4	0.0	0.0	18.983750688218958	0.0
36	5.065088925233716E-4	0.0	0.0	19.422083483808684	0.0
37	5.065088925233716E-4	0.0	0.0	19.861631900740466	0.0
38	5.065088925233716E-4	0.0	0.0	20.30107901589374	0.0
39	5.065088925233716E-4	0.0	0.0	20.767978913021786	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGCGA	25	3.8918275E-5	45.0	42
GTGCGAT	20	7.0345186E-4	45.0	26
ATATGCG	70	0.0	41.785713	1
AATGCGA	130	0.0	39.807693	25
TCTAGCG	30	1.1402185E-4	37.500004	1
CGACGGT	180	0.0	37.5	28
AATACGG	90	0.0	37.5	2
TCTACGG	140	0.0	36.964283	2
GCGTAAG	130	0.0	36.346157	1
CGGGTAT	205	0.0	36.219513	6
TGATTCG	205	0.0	36.219513	15
CGCATCG	75	1.8189894E-12	36.0	21
TAAATCG	25	0.0021074745	36.0	39
ATGCGAA	145	0.0	35.689655	26
GTTAACG	70	2.1827873E-11	35.357143	1
CACGACG	185	0.0	35.27027	26
TTAGGCG	115	0.0	35.217392	1
CGGTCTA	200	0.0	34.875	31
TAGGGAC	1265	0.0	34.683792	5
TGTTACG	65	3.6743586E-10	34.615387	1
>>END_MODULE