FastQCFastQC Report
Sat 18 Jun 2016
SRR3549939_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549939_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1520187
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC42150.27726852025441606TruSeq Adapter, Index 23 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC40440.26601990413021553TruSeq Adapter, Index 23 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT23850.15688859331121763No Hit
CTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCT23240.15287592907977768TruSeq Adapter, Index 20 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17130.11268350538453492No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT207.034005E-445.013
TTAGTCG253.8914015E-545.039
CGATCAT351.2122837E-745.010
TATCCGA207.034005E-445.025
CGGTCTA950.042.6315831
CACTACG603.6379788E-1241.2500041
AACGGGC2750.040.9090924
GTTAACG556.184564E-1140.9090921
TATAACG556.184564E-1140.9090921
CGACGGT1000.040.528
CGAATAT4200.040.17857414
TCGATAG451.9290383E-840.01
GCTACGA4350.039.82758310
CGTAAGG1500.039.02
TAAGGGT4950.038.6363644
ATGCGAA2250.038.026
GACCTAT4000.037.687533
ACGGGTG2150.037.6744165
GCGATCA2100.037.5000049
GCGATAA1200.037.5000049
Produced by FastQC (version 0.11.5)