##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549939_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1520187
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.612186527052266	34.0	31.0	34.0	31.0	34.0
2	32.791733516994945	34.0	31.0	34.0	31.0	34.0
3	32.79221898358557	34.0	31.0	34.0	31.0	34.0
4	36.2010778937065	37.0	37.0	37.0	35.0	37.0
5	36.20971235775599	37.0	37.0	37.0	35.0	37.0
6	36.19905972094222	37.0	37.0	37.0	35.0	37.0
7	36.303635671137826	37.0	37.0	37.0	35.0	37.0
8	36.28446105643582	37.0	37.0	37.0	35.0	37.0
9	37.68433883463021	39.0	38.0	39.0	35.0	39.0
10	37.684829563731306	39.0	37.0	39.0	35.0	39.0
11	37.638628668709835	39.0	37.0	39.0	35.0	39.0
12	37.494758210667506	39.0	37.0	39.0	35.0	39.0
13	37.44973743361837	39.0	37.0	39.0	35.0	39.0
14	38.70519350579896	40.0	38.0	41.0	35.0	41.0
15	38.72589687979176	40.0	38.0	41.0	35.0	41.0
16	38.751319410046264	40.0	38.0	41.0	35.0	41.0
17	38.706182199953034	40.0	38.0	41.0	35.0	41.0
18	38.646423762339765	40.0	38.0	41.0	35.0	41.0
19	38.63080331564472	40.0	38.0	41.0	35.0	41.0
20	38.608046904755795	40.0	38.0	41.0	35.0	41.0
21	38.572583504529376	40.0	38.0	41.0	35.0	41.0
22	38.53118004561281	40.0	38.0	41.0	35.0	41.0
23	38.496179746307526	40.0	38.0	41.0	35.0	41.0
24	38.488297163441075	40.0	37.0	41.0	35.0	41.0
25	38.41792555784256	40.0	37.0	41.0	35.0	41.0
26	38.325403387872676	40.0	37.0	41.0	34.0	41.0
27	38.24493828719756	40.0	37.0	41.0	34.0	41.0
28	38.15429483346457	40.0	37.0	41.0	34.0	41.0
29	38.1031096832166	40.0	37.0	41.0	34.0	41.0
30	38.0096178956931	40.0	36.0	41.0	34.0	41.0
31	37.88480890837772	40.0	36.0	41.0	34.0	41.0
32	37.789521289157186	40.0	36.0	41.0	33.0	41.0
33	37.68400729647076	40.0	36.0	41.0	33.0	41.0
34	37.55846682020041	40.0	36.0	41.0	33.0	41.0
35	37.50736521230612	40.0	36.0	41.0	33.0	41.0
36	37.389580360837186	40.0	35.0	41.0	33.0	41.0
37	37.3014497558524	40.0	35.0	41.0	33.0	41.0
38	37.1805389731658	40.0	35.0	41.0	32.0	41.0
39	37.10715326469704	39.0	35.0	41.0	32.0	41.0
40	36.98018730590381	39.0	35.0	41.0	32.0	41.0
41	36.87834128301321	39.0	35.0	41.0	32.0	41.0
42	36.74994063230379	39.0	35.0	41.0	31.0	41.0
43	36.66981956825048	39.0	35.0	41.0	31.0	41.0
44	36.559588392743784	39.0	35.0	41.0	31.0	41.0
45	36.42705272443456	39.0	35.0	41.0	31.0	41.0
46	36.28431436395654	38.0	35.0	41.0	31.0	41.0
47	36.19954321409143	38.0	35.0	40.0	30.0	41.0
48	36.073811971816625	38.0	35.0	40.0	30.0	41.0
49	35.95972206050966	38.0	35.0	40.0	30.0	41.0
50	35.81157975959537	38.0	35.0	40.0	30.0	41.0
51	34.11380442011411	36.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	19.0
9	57.0
10	74.0
11	47.0
12	33.0
13	37.0
14	63.0
15	71.0
16	123.0
17	227.0
18	357.0
19	675.0
20	1098.0
21	1722.0
22	2576.0
23	3750.0
24	5475.0
25	7467.0
26	9190.0
27	11043.0
28	13102.0
29	15057.0
30	18011.0
31	22773.0
32	29933.0
33	43080.0
34	91274.0
35	120424.0
36	98994.0
37	159049.0
38	309333.0
39	554791.0
40	259.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.92210497787443	19.810128622333963	24.2480694809257	11.019696918865902
2	33.03041007455004	23.773983069188198	24.990807052027154	18.20479980423461
3	32.66742841505683	24.235505237184636	25.721309286291756	17.37575706146678
4	29.256137567286128	26.1015256675659	24.320955250900052	20.321381514247918
5	25.125987789660087	30.598340862012368	23.06439931403176	21.21127203429578
6	25.86379175719829	35.797635422484205	23.275886453442897	15.062686366874601
7	82.37506306789888	5.806917175321194	7.207534336236265	4.610485420543657
8	82.1723248521399	5.428411109948973	7.2889059043394	5.110358133571725
9	75.36678053423691	7.549071265574564	9.858984453886265	7.225163746302264
10	40.34832556784133	25.35635418537325	17.70446662154064	16.59085362524479
11	31.573220926109748	25.656119937876063	22.69391857712242	20.076740558891768
12	28.09976667344215	22.722993947455148	29.004918473845652	20.172320905257052
13	25.82379667764558	24.35154359299218	29.163517383058796	20.66114234630345
14	21.57024102955755	27.53345476576237	28.056548306228112	22.839755898451966
15	20.40268730097021	25.61448032380227	33.013635822435006	20.969196552792518
16	23.08143669167017	23.657944713380658	30.739244579778674	22.521374015170505
17	23.705833558634563	23.577165177705112	27.370251159890195	25.346750103770127
18	23.529802583497954	25.519097321579515	28.882959793762215	22.06814030116032
19	24.971006856393323	26.416881607328573	25.964568832650194	22.64754270362791
20	26.426551470312532	25.17335038386725	26.29952762390416	22.100570521916055
21	26.066529972957277	26.32807674319015	26.5037130300417	21.10168025381088
22	23.482834677575852	24.77859631742674	25.933651583653855	25.80491742134356
23	22.943098447756757	26.032784124584673	26.899848505479916	24.124268922178654
24	23.593478960154243	24.687357542197113	28.201662032368386	23.517501465280258
25	23.6805077270099	25.31385941334849	26.074818426943523	24.93081443269808
26	22.521900266217248	26.0721871717098	26.14171809126114	25.264194470811812
27	23.1643212315327	25.879118818934778	27.4920124958311	23.46454745370142
28	21.480646788849004	26.340443642788685	28.141735194420157	24.037174373942154
29	23.576836270800893	25.595337941976876	25.794326619027792	25.03349916819444
30	25.30846534011934	24.901278592699448	26.0911979907735	23.699058076407706
31	24.84608801417194	26.79742689550693	24.93581381764217	23.420671272678952
32	24.846745827980374	26.379386220247902	25.26406290805013	23.509805043721595
33	24.71057836963479	26.241705790142923	24.886148875105498	24.16156696511679
34	22.373300126892286	25.83964999042881	27.92136756859518	23.865682314083728
35	23.83700163203606	23.883048598626353	26.79051985051839	25.489429918819194
36	24.136833165919718	27.803750459647397	25.53435860193516	22.525057772497725
37	22.823310553241146	28.263036060695164	27.027990635362624	21.885662750701066
38	21.716670383314685	28.474325855963773	25.526464836233963	24.28253892448758
39	22.448817152100368	25.754660446379297	26.605411044825406	25.191111356694933
40	23.787599815022755	24.517641579621454	28.66252638655639	23.032232218799397
41	21.204101863783865	25.841031399426516	27.32315169120641	25.63171504558321
42	22.526636525637965	26.738552559652202	26.378925750582	24.355885164127834
43	23.368111949385174	24.88318871296755	26.549167964204408	25.199531373442873
44	22.4631574931242	24.779319912616014	26.267623654195177	26.48989894006461
45	22.81673241515682	24.03934515950998	26.576204111730988	26.567718313602207
46	23.18984440729989	25.14999799366788	26.96247238004272	24.697685218989506
47	22.150038120310196	25.143617199726087	29.19219806510646	23.51414661485725
48	21.13924142227239	24.686370821484463	28.912495633760848	25.261892122482298
49	21.81409260834358	24.812407947180183	28.800864630469803	24.572634814006435
50	21.231861606499727	23.79246763720516	28.779617244457423	26.196053511837686
51	20.97005171074348	24.031846082093846	27.40123419026738	27.596868016895286
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	537.0
1	1524.5
2	2512.0
3	3284.0
4	4056.0
5	3128.0
6	2200.0
7	2032.5
8	1865.0
9	1933.5
10	2002.0
11	2143.5
12	2285.0
13	2326.5
14	2368.0
15	2492.5
16	2617.0
17	2985.0
18	3353.0
19	3054.5
20	2756.0
21	3214.0
22	3672.0
23	4670.5
24	5669.0
25	6361.5
26	9185.5
27	11317.0
28	13765.5
29	16214.0
30	19411.0
31	22608.0
32	25027.0
33	27446.0
34	32200.0
35	36954.0
36	37839.0
37	38724.0
38	43673.0
39	48622.0
40	53837.5
41	59053.0
42	66638.5
43	74224.0
44	83987.0
45	93750.0
46	105553.5
47	117357.0
48	130414.5
49	143472.0
50	143137.5
51	142803.0
52	127282.5
53	111762.0
54	102486.5
55	93211.0
56	87208.5
57	81206.0
58	79236.0
59	77266.0
60	74734.0
61	72202.0
62	66867.0
63	61532.0
64	54380.5
65	47229.0
66	39622.5
67	32016.0
68	27285.5
69	22555.0
70	19476.5
71	16398.0
72	14155.0
73	11912.0
74	9416.5
75	6047.5
76	5174.0
77	3870.0
78	2566.0
79	1893.0
80	1220.0
81	1008.5
82	797.0
83	573.0
84	349.0
85	283.0
86	217.0
87	156.0
88	95.0
89	58.5
90	22.0
91	19.0
92	16.0
93	16.0
94	16.0
95	11.0
96	6.0
97	4.0
98	2.0
99	4.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1520187.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.857300364709104
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.0024881250298	16.939276858405268
2	11.409990965676474	5.444231632535219
3	4.387799642200548	3.1404316201242484
4	2.1637847232228604	2.0648824826598697
5	1.330502960241043	1.5871104379302587
6	0.8841814341175276	1.2656509230384665
7	0.6192050003588743	1.0340791776624032
8	0.488791376938127	0.9328994156234114
9	0.39459588003138457	0.8472593189326914
>10	5.5027039876657735	33.97343949467172
>50	1.7077172046887907	26.542205418841885
>100	0.10074525139140793	4.0757698179932795
>500	0.00444056203691344	0.7221785261601843
>1k	0.0030528864003779893	1.4305848754210087
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	4215	0.27726852025441606	TruSeq Adapter, Index 23 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	4044	0.26601990413021553	TruSeq Adapter, Index 23 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2385	0.15688859331121763	No Hit
CTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCT	2324	0.15287592907977768	TruSeq Adapter, Index 20 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1713	0.11268350538453492	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.973441425298335E-4	0.0	0.0	0.17168940400095514	0.0
2	1.973441425298335E-4	0.0	0.0	0.960802848596916	0.0
3	1.973441425298335E-4	0.0	0.0	1.3347699986909505	0.0
4	1.973441425298335E-4	0.0	0.0	1.7185385745306334	0.0
5	1.973441425298335E-4	0.0	0.0	2.7901172684676294	0.0
6	1.973441425298335E-4	0.0	0.0	3.440168873960901	0.0
7	1.973441425298335E-4	0.0	0.0	4.06969668863107	0.0
8	1.973441425298335E-4	0.0	0.0	5.1350261513879545	0.0
9	1.973441425298335E-4	0.0	0.0	5.555961207404089	0.0
10	1.973441425298335E-4	0.0	0.0	6.474203502595405	0.0
11	3.2890690421638917E-4	0.0	0.0	7.653861005257906	0.0
12	3.2890690421638917E-4	0.0	0.0	8.555592173857558	0.0
13	3.2890690421638917E-4	0.0	0.0	8.920547274776064	0.0
14	3.2890690421638917E-4	0.0	0.0	9.068950069958499	0.0
15	3.94688285059667E-4	0.0	0.0	9.391147273328873	0.0
16	3.94688285059667E-4	0.0	0.0	10.07481316443306	0.0
17	3.94688285059667E-4	0.0	0.0	10.877280229340206	0.0
18	3.94688285059667E-4	0.0	0.0	11.801837537092476	0.0
19	3.94688285059667E-4	0.0	0.0	12.25447921867507	0.0
20	4.6046966590294484E-4	0.0	0.0	12.713567475580307	0.0
21	4.6046966590294484E-4	0.0	0.0	13.29862707680042	6.578138084327784E-5
22	4.6046966590294484E-4	0.0	0.0	13.914603927016874	6.578138084327784E-5
23	4.6046966590294484E-4	0.0	0.0	14.55932724066184	6.578138084327784E-5
24	4.6046966590294484E-4	0.0	0.0	15.063081055159662	6.578138084327784E-5
25	4.6046966590294484E-4	0.0	0.0	15.524603223156099	6.578138084327784E-5
26	4.6046966590294484E-4	0.0	0.0	15.92961918500816	6.578138084327784E-5
27	4.6046966590294484E-4	0.0	0.0	16.338450466949133	6.578138084327784E-5
28	4.6046966590294484E-4	0.0	0.0	16.76583209828791	6.578138084327784E-5
29	4.6046966590294484E-4	0.0	0.0	17.24787805710745	6.578138084327784E-5
30	4.6046966590294484E-4	0.0	0.0	17.83392437904021	6.578138084327784E-5
31	4.6046966590294484E-4	0.0	0.0	18.294196700800626	6.578138084327784E-5
32	4.6046966590294484E-4	0.0	0.0	18.75282448803996	6.578138084327784E-5
33	5.262510467462227E-4	0.0	0.0	19.19316505140486	6.578138084327784E-5
34	5.262510467462227E-4	0.0	0.0	19.632979363723017	6.578138084327784E-5
35	5.262510467462227E-4	0.0	0.0	20.11713032672954	6.578138084327784E-5
36	5.262510467462227E-4	0.0	0.0	20.56023370808986	6.578138084327784E-5
37	5.262510467462227E-4	0.0	0.0	21.02340041060738	6.578138084327784E-5
38	5.262510467462227E-4	0.0	0.0	21.459004714551565	6.578138084327784E-5
39	5.262510467462227E-4	0.0	0.0	21.928354866868354	6.578138084327784E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTACGT	20	7.034005E-4	45.0	13
TTAGTCG	25	3.8914015E-5	45.0	39
CGATCAT	35	1.2122837E-7	45.0	10
TATCCGA	20	7.034005E-4	45.0	25
CGGTCTA	95	0.0	42.63158	31
CACTACG	60	3.6379788E-12	41.250004	1
AACGGGC	275	0.0	40.909092	4
GTTAACG	55	6.184564E-11	40.909092	1
TATAACG	55	6.184564E-11	40.909092	1
CGACGGT	100	0.0	40.5	28
CGAATAT	420	0.0	40.178574	14
TCGATAG	45	1.9290383E-8	40.0	1
GCTACGA	435	0.0	39.827583	10
CGTAAGG	150	0.0	39.0	2
TAAGGGT	495	0.0	38.636364	4
ATGCGAA	225	0.0	38.0	26
GACCTAT	400	0.0	37.6875	33
ACGGGTG	215	0.0	37.674416	5
GCGATCA	210	0.0	37.500004	9
GCGATAA	120	0.0	37.500004	9
>>END_MODULE