SRR3549936_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549936_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	997028
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC	3955	0.39667892977930413	No Hit
CCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC	3285	0.329479212218714	No Hit
CTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGCT	2184	0.21905101963034138	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC	1426	0.1430250705095544	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1233	0.123667539928668	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	1142	0.11454041411073711	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	1102	0.11052849067428397	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGATA	20	7.032835E-4	45.000004	25
CGACCGT	20	7.032835E-4	45.000004	34
AATGCGT	20	7.032835E-4	45.000004	21
CGTAGGA	20	7.032835E-4	45.000004	3
TACCTCG	20	7.032835E-4	45.000004	30
ACTCGGT	20	7.032835E-4	45.000004	21
CGTACAG	20	7.032835E-4	45.000004	37
AATTACG	40	6.8139343E-9	45.000004	1
ATACGTA	20	7.032835E-4	45.000004	37
CTCTCGT	20	7.032835E-4	45.000004	40
CGCTTTA	20	7.032835E-4	45.000004	40
AATCGAT	20	7.032835E-4	45.000004	42
AACACGA	20	7.032835E-4	45.000004	14
AAACGGC	50	2.1827873E-11	45.0	25
TAGGACG	50	2.1827873E-11	45.0	1
ATCATAC	25	3.8904334E-5	45.0	39
TATTACG	30	2.1652122E-6	44.999996	1
CGGTCTA	60	0.0	44.999996	31
GTTACGT	75	0.0	42.000004	1
ACACGAC	65	0.0	41.538464	26