##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549936_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	997028
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.55496435406027	34.0	31.0	34.0	31.0	34.0
2	32.73226128052572	34.0	31.0	34.0	31.0	34.0
3	32.74186883417517	34.0	31.0	34.0	31.0	34.0
4	36.15405685697894	37.0	35.0	37.0	35.0	37.0
5	36.17256987767645	37.0	35.0	37.0	35.0	37.0
6	36.167430603754354	37.0	36.0	37.0	35.0	37.0
7	36.292582555354514	37.0	37.0	37.0	35.0	37.0
8	36.282338108859534	37.0	37.0	37.0	35.0	37.0
9	37.68640700160878	39.0	38.0	39.0	35.0	39.0
10	37.65041102155607	39.0	37.0	39.0	35.0	39.0
11	37.63790986812808	39.0	37.0	39.0	35.0	39.0
12	37.52482076732048	39.0	37.0	39.0	35.0	39.0
13	37.43784627914161	39.0	37.0	39.0	35.0	39.0
14	38.679945798914375	40.0	38.0	41.0	35.0	41.0
15	38.73896721054975	40.0	38.0	41.0	35.0	41.0
16	38.773046494180704	40.0	38.0	41.0	35.0	41.0
17	38.72195966412177	40.0	38.0	41.0	35.0	41.0
18	38.68086051745789	40.0	38.0	41.0	35.0	41.0
19	38.650270604235786	40.0	38.0	41.0	35.0	41.0
20	38.614643721139224	40.0	38.0	41.0	35.0	41.0
21	38.58554423747377	40.0	38.0	41.0	35.0	41.0
22	38.54632567992073	40.0	38.0	41.0	35.0	41.0
23	38.54082332692763	40.0	38.0	41.0	35.0	41.0
24	38.52623597331269	40.0	38.0	41.0	35.0	41.0
25	38.446561179826446	40.0	37.0	41.0	35.0	41.0
26	38.37225333691732	40.0	37.0	41.0	34.0	41.0
27	38.30142884653189	40.0	37.0	41.0	34.0	41.0
28	38.1889455461632	40.0	37.0	41.0	34.0	41.0
29	38.11901671768496	40.0	37.0	41.0	34.0	41.0
30	38.01822516519095	40.0	37.0	41.0	34.0	41.0
31	37.881349370328614	40.0	36.0	41.0	34.0	41.0
32	37.79275005315799	40.0	36.0	41.0	33.0	41.0
33	37.6620646561581	40.0	36.0	41.0	33.0	41.0
34	37.555839956350276	40.0	36.0	41.0	33.0	41.0
35	37.52926698146893	40.0	36.0	41.0	33.0	41.0
36	37.37402961601881	40.0	35.0	41.0	33.0	41.0
37	37.26333864244535	40.0	35.0	41.0	33.0	41.0
38	37.09843855939853	39.0	35.0	41.0	32.0	41.0
39	37.03030306069639	39.0	35.0	41.0	32.0	41.0
40	36.934979759846264	39.0	35.0	41.0	31.0	41.0
41	36.86905583393847	39.0	35.0	41.0	31.0	41.0
42	36.705610073137365	39.0	35.0	41.0	31.0	41.0
43	36.61330173274973	39.0	35.0	41.0	31.0	41.0
44	36.491677264831075	39.0	35.0	41.0	31.0	41.0
45	36.381272140802466	39.0	35.0	41.0	31.0	41.0
46	36.259346778626075	39.0	35.0	41.0	30.0	41.0
47	36.169789614734995	38.0	35.0	40.0	30.0	41.0
48	36.02738839831981	38.0	35.0	40.0	30.0	41.0
49	35.93556148874455	38.0	35.0	40.0	30.0	41.0
50	35.784672045318686	38.0	35.0	40.0	29.0	41.0
51	34.07382540911589	36.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	15.0
9	29.0
10	34.0
11	35.0
12	29.0
13	14.0
14	31.0
15	47.0
16	75.0
17	114.0
18	216.0
19	401.0
20	660.0
21	1110.0
22	1774.0
23	2561.0
24	3674.0
25	4925.0
26	6106.0
27	7400.0
28	8505.0
29	10159.0
30	12262.0
31	15236.0
32	20179.0
33	28221.0
34	55643.0
35	80397.0
36	66588.0
37	106269.0
38	205852.0
39	358252.0
40	214.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.00227476058846	20.1145805333451	25.187055930224627	10.696088775841801
2	32.716433239588056	23.773354409304453	25.222962644980885	18.28724970612661
3	33.093955234958294	23.82972193358662	26.464051160047664	16.612271671407424
4	29.421942011658647	25.70288898606659	24.618466081193308	20.256702921081455
5	23.77856990977184	31.013271442727785	23.50004212519608	21.708116522304287
6	25.368194273380485	35.81273544975668	23.77455798633539	15.044512290527448
7	81.99739626168974	5.8206991177780365	7.5865472183328855	4.595357402199336
8	81.86610606723181	5.1429849512751895	7.428176540678898	5.5627324408140995
9	74.76570367131113	7.592865997745299	10.226493137604962	7.414937193338603
10	37.79623039673911	27.068146531491593	18.515829043918526	16.619794027850773
11	28.90901760030812	25.860457279033287	24.479051741776562	20.75147337888204
12	25.96566997115427	22.083231363612658	31.61907188163221	20.332026783600863
13	24.989067508635664	24.67001929735173	31.572032079339795	18.76888111467281
14	20.60694383708381	29.082633587020624	28.6450330381895	21.665389537706062
15	18.950019457828667	25.875501991919986	34.104458450514926	21.070020099736418
16	21.56860188480163	24.222188343757647	31.1718427165536	23.037367054887127
17	21.32618141115395	24.02901423029243	27.862908564252958	26.781895794300663
18	22.906879245116485	24.56962091335449	30.144589720649773	22.378910120879254
19	23.510774020388595	26.0925470498321	27.394917695390703	23.001761234388603
20	24.175950926152527	25.699880043489248	27.30735746639011	22.816811563968116
21	23.94526532855647	26.29484828911525	28.770907136008216	20.988979246320064
22	21.05387210790469	24.275446627376564	27.58708882799681	27.083592436721936
23	20.422997147522437	27.05059436645711	27.461515624435823	25.06489286158463
24	22.31993484636339	25.265489033407285	28.698792812237972	23.71578330799135
25	21.443429873584293	26.447903168215937	26.98088719674874	25.12777976145103
26	20.727000645919674	27.243066393320948	26.876577187400958	25.15335577335842
27	22.76987205976161	26.02895806336432	28.129500876605274	23.0716690002688
28	20.453086573295835	26.143297881303234	29.90517818957943	23.4984373558215
29	22.536679010017775	25.322959836634478	28.037527531824587	24.102833621523168
30	24.532410323481386	25.81702820783368	26.925522653325686	22.725038815359248
31	25.035104330068965	27.666825806296313	24.47353534705144	22.824534516583288
32	25.023269155931427	26.93194173082401	25.124169030358225	22.92062008288634
33	23.468548526219926	26.674075351945987	25.87379692445949	23.983579197374596
34	21.204018342513955	26.19003678933791	27.29742795588489	25.308516912263247
35	23.969035974917453	23.657710716248694	27.2240097569978	25.149243551836058
36	25.269099764500098	26.049117978632495	26.889615938569428	21.79216631829798
37	22.538083183220532	26.54880304264273	28.191083901354823	22.722029872781906
38	20.651576485314354	29.372795949562096	26.92692682652844	23.048700738595105
39	21.244839663479862	25.494268967371024	28.731389690159155	24.52950167898996
40	23.771047553328494	23.3541084101951	28.70621487059541	24.168629165880997
41	21.833188235435717	23.853693176119428	27.183589628375532	27.129528960069326
42	23.248594823816383	24.498810464701094	28.113954673289015	24.13864003819351
43	23.814978114957654	23.51267968402091	28.521265200174923	24.151077000846517
44	22.04130676370172	24.706427502537544	28.608023044488217	24.64424268927252
45	22.434776154731864	24.620772937169267	26.96584248386204	25.978608424236832
46	24.320179573693014	24.805321415246112	27.62450001404173	23.24999899701914
47	22.377405649590585	24.426696140930844	29.629258155237366	23.566640054241205
48	21.530388314069416	24.382665281215772	29.579410006539437	24.50753639817538
49	21.58655524217976	23.186610606723182	31.34014290471281	23.886691246384252
50	20.701524932098195	23.060937105076288	31.255391022117735	24.982146940707782
51	21.771204018342512	22.91329832261481	27.46693172107503	27.84856593796764
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	544.0
1	1046.0
2	1548.0
3	2040.0
4	2532.0
5	1959.5
6	1387.0
7	1309.0
8	1231.0
9	1322.5
10	1414.0
11	1453.5
12	1493.0
13	1620.5
14	1748.0
15	1800.0
16	1852.0
17	1870.0
18	1888.0
19	1960.5
20	2033.0
21	2369.5
22	2706.0
23	3325.5
24	3945.0
25	4815.0
26	6765.0
27	7845.0
28	9521.5
29	11198.0
30	12565.5
31	13933.0
32	15638.0
33	17343.0
34	20104.0
35	22865.0
36	24219.0
37	25573.0
38	28351.5
39	31130.0
40	35551.0
41	39972.0
42	46608.5
43	53245.0
44	62479.5
45	71714.0
46	81035.5
47	90357.0
48	100716.0
49	111075.0
50	107743.0
51	104411.0
52	89922.5
53	75434.0
54	66329.0
55	57224.0
56	52901.0
57	48578.0
58	45018.0
59	41458.0
60	38164.0
61	34870.0
62	32247.0
63	29624.0
64	26759.0
65	23894.0
66	21107.5
67	18321.0
68	15527.0
69	12733.0
70	10778.5
71	8824.0
72	7777.0
73	6730.0
74	5356.0
75	2955.0
76	1928.0
77	1660.5
78	1393.0
79	942.5
80	492.0
81	433.0
82	374.0
83	254.0
84	134.0
85	145.5
86	157.0
87	121.0
88	85.0
89	61.5
90	38.0
91	43.5
92	49.0
93	42.0
94	35.0
95	19.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	997028.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.41072221346529
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.46669205779229	18.61076229240153
2	11.926103183342553	6.299539965287686
3	4.682789061440467	3.7102752325787405
4	2.397657446012789	2.532954590787617
5	1.4431576592167714	1.9057418023904478
6	0.939926150228052	1.4894477072906958
7	0.6842377639472625	1.2649849458101652
8	0.5245979234456966	1.1084008023908019
9	0.41236467876833516	0.9801764083435814
>10	5.207467818709933	35.08387769574946
>50	1.218870033043664	20.40420691735873
>100	0.08736188614272858	4.104962001509329
>500	0.0061038872414133265	1.0611521231136458
>1k	0.00267045066811833	1.4435175149875812
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC	3955	0.39667892977930413	No Hit
CCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC	3285	0.329479212218714	No Hit
CTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGCT	2184	0.21905101963034138	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC	1426	0.1430250705095544	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1233	0.123667539928668	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	1142	0.11454041411073711	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	1102	0.11052849067428397	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0029808591132847E-4	0.0	0.0	0.2445267334518188	0.0
2	1.0029808591132847E-4	0.0	0.0	1.3447967358990922	0.0
3	1.0029808591132847E-4	0.0	0.0	1.8461868673698232	0.0
4	1.0029808591132847E-4	0.0	0.0	2.407755850387351	0.0
5	1.0029808591132847E-4	0.0	0.0	3.9302807945213174	0.0
6	1.0029808591132847E-4	0.0	0.0	4.775994254925639	0.0
7	1.0029808591132847E-4	0.0	0.0	5.623111888532719	0.0
8	1.0029808591132847E-4	0.0	0.0	7.080242480652499	0.0
9	1.0029808591132847E-4	0.0	0.0	7.593267190088945	0.0
10	1.0029808591132847E-4	0.0	0.0	8.75863065029267	0.0
11	1.0029808591132847E-4	0.0	0.0	10.31926886707294	0.0
12	1.0029808591132847E-4	0.0	0.0	11.542103130503857	0.0
13	1.0029808591132847E-4	0.0	0.0	12.016613374950353	0.0
14	1.0029808591132847E-4	0.0	0.0	12.192135025295178	0.0
15	1.0029808591132847E-4	0.0	0.0	12.559827808246107	0.0
16	1.0029808591132847E-4	0.0	0.0	13.394408181114272	0.0
17	1.0029808591132847E-4	0.0	0.0	14.45726699751662	0.0
18	1.0029808591132847E-4	0.0	0.0	15.744793526360343	0.0
19	1.0029808591132847E-4	0.0	0.0	16.33564955046398	0.0
20	1.0029808591132847E-4	0.0	0.0	16.934128229096874	0.0
21	1.0029808591132847E-4	0.0	0.0	17.649755072074203	0.0
22	1.0029808591132847E-4	0.0	0.0	18.388651070982963	0.0
23	1.0029808591132847E-4	0.0	0.0	19.19043396975812	0.0
24	1.0029808591132847E-4	0.0	0.0	19.769555117810132	0.0
25	1.0029808591132847E-4	0.0	0.0	20.300733780796527	0.0
26	1.0029808591132847E-4	0.0	0.0	20.805634345274154	0.0
27	1.0029808591132847E-4	0.0	0.0	21.313543852329122	0.0
28	1.0029808591132847E-4	0.0	0.0	21.85685858371079	0.0
29	1.0029808591132847E-4	0.0	0.0	22.4519271274227	0.0
30	1.0029808591132847E-4	0.0	0.0	23.153512238372443	0.0
31	1.0029808591132847E-4	0.0	0.0	23.734438751970856	0.0
32	1.0029808591132847E-4	0.0	0.0	24.272036492455577	0.0
33	1.0029808591132847E-4	0.0	0.0	24.82718639797478	0.0
34	1.0029808591132847E-4	0.0	0.0	25.374212158535165	0.0
35	1.0029808591132847E-4	0.0	0.0	25.978006635721364	0.0
36	1.0029808591132847E-4	0.0	0.0	26.517409741752488	0.0
37	1.0029808591132847E-4	0.0	0.0	27.057615232470905	0.0
38	1.0029808591132847E-4	0.0	0.0	27.568032191673655	0.0
39	1.0029808591132847E-4	0.0	0.0	28.12789610723069	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGATA	20	7.032835E-4	45.000004	25
CGACCGT	20	7.032835E-4	45.000004	34
AATGCGT	20	7.032835E-4	45.000004	21
CGTAGGA	20	7.032835E-4	45.000004	3
TACCTCG	20	7.032835E-4	45.000004	30
ACTCGGT	20	7.032835E-4	45.000004	21
CGTACAG	20	7.032835E-4	45.000004	37
AATTACG	40	6.8139343E-9	45.000004	1
ATACGTA	20	7.032835E-4	45.000004	37
CTCTCGT	20	7.032835E-4	45.000004	40
CGCTTTA	20	7.032835E-4	45.000004	40
AATCGAT	20	7.032835E-4	45.000004	42
AACACGA	20	7.032835E-4	45.000004	14
AAACGGC	50	2.1827873E-11	45.0	25
TAGGACG	50	2.1827873E-11	45.0	1
ATCATAC	25	3.8904334E-5	45.0	39
TATTACG	30	2.1652122E-6	44.999996	1
CGGTCTA	60	0.0	44.999996	31
GTTACGT	75	0.0	42.000004	1
ACACGAC	65	0.0	41.538464	26
>>END_MODULE