FastQCFastQC Report
Sat 18 Jun 2016
SRR3549935_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549935_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences752039
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC42970.5713799417317452TruSeq Adapter, Index 15 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC37370.49691571846672844TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCT24380.32418531485734114TruSeq Adapter, Index 14 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC15080.20052151550650965TruSeq Adapter, Index 15 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCG12350.16422020666481393No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGC12260.1630234602194833No Hit
GGCAAGGTGGGGTGAGGCCCGGAGTTGGGGAAGCTAGGAGGCTTAAAGCCT12250.16289048839222434No Hit
GAATCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTC11190.14879547470277474No Hit
ACTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC10580.14068419323997824TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9090.12087139097839342No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTAC207.031728E-445.00000413
GTCGCAA207.031728E-445.00000414
TACCACG207.031728E-445.0000042
ATTAAGT207.031728E-445.00000413
CGACATT207.031728E-445.00000426
ACCCTTA207.031728E-445.00000421
GCCGATA351.2111741E-745.0000049
AGTGCCG207.031728E-445.00000410
CCCCGTA207.031728E-445.00000443
CGCTAGG650.045.0000042
ACGGACC207.031728E-445.00000421
TTGCGTA207.031728E-445.00000427
CGTACTT207.031728E-445.00000415
ATGACGG700.045.0000042
TACTCCG207.031728E-445.00000414
ATAACGC406.8102963E-945.00000411
TAGCTAA207.031728E-445.00000411
TAAGACG207.031728E-445.0000041
GCGTATG750.045.0000041
AGTACGG850.045.0000042
Produced by FastQC (version 0.11.5)