##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549935_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	752039
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.4887233241893	33.0	31.0	34.0	31.0	34.0
2	32.669749840101375	34.0	31.0	34.0	31.0	34.0
3	32.68595112753461	34.0	31.0	34.0	31.0	34.0
4	36.116040524494075	37.0	35.0	37.0	35.0	37.0
5	36.12424488623596	37.0	35.0	37.0	35.0	37.0
6	36.116718680813094	37.0	35.0	37.0	35.0	37.0
7	36.23334561106538	37.0	36.0	37.0	35.0	37.0
8	36.225396555231846	37.0	37.0	37.0	35.0	37.0
9	37.73878216422287	39.0	38.0	39.0	35.0	39.0
10	37.629166838421945	39.0	37.0	39.0	35.0	39.0
11	37.5379481649223	39.0	37.0	39.0	35.0	39.0
12	37.402703849135484	39.0	37.0	39.0	35.0	39.0
13	37.301296874231255	39.0	37.0	39.0	35.0	39.0
14	38.50741650366537	40.0	38.0	41.0	35.0	41.0
15	38.56164374453984	40.0	38.0	41.0	35.0	41.0
16	38.59968299516382	40.0	38.0	41.0	35.0	41.0
17	38.560028136838646	40.0	38.0	41.0	35.0	41.0
18	38.49640111749524	40.0	37.0	41.0	35.0	41.0
19	38.43802781504682	40.0	37.0	41.0	34.0	41.0
20	38.391177851148676	40.0	37.0	41.0	34.0	41.0
21	38.41399315726977	40.0	37.0	41.0	34.0	41.0
22	38.382843177016085	40.0	37.0	41.0	34.0	41.0
23	38.334067781059225	40.0	37.0	41.0	34.0	41.0
24	38.28173937787801	40.0	37.0	41.0	34.0	41.0
25	38.149678407635776	40.0	36.0	41.0	34.0	41.0
26	38.03833710751703	40.0	36.0	41.0	34.0	41.0
27	38.0550702822593	40.0	36.0	41.0	34.0	41.0
28	38.03822807061868	40.0	36.0	41.0	34.0	41.0
29	38.02120235785644	40.0	36.0	41.0	34.0	41.0
30	37.89848531791569	40.0	36.0	41.0	34.0	41.0
31	37.770876244450086	40.0	36.0	41.0	34.0	41.0
32	37.66392301463089	40.0	36.0	41.0	33.0	41.0
33	37.57216048635775	40.0	36.0	41.0	33.0	41.0
34	37.4756255992043	40.0	35.0	41.0	33.0	41.0
35	37.43741880407798	40.0	35.0	41.0	33.0	41.0
36	37.348823664730155	40.0	35.0	41.0	33.0	41.0
37	37.20364236429228	39.0	35.0	41.0	33.0	41.0
38	37.04730073839256	39.0	35.0	41.0	32.0	41.0
39	36.9537683550986	39.0	35.0	41.0	32.0	41.0
40	36.80747541018484	39.0	35.0	41.0	31.0	41.0
41	36.72607005753691	39.0	35.0	41.0	31.0	41.0
42	36.61138318624433	39.0	35.0	41.0	31.0	41.0
43	36.5316705649574	39.0	35.0	41.0	31.0	41.0
44	36.443917137276124	39.0	35.0	41.0	31.0	41.0
45	36.29090911508579	38.0	35.0	40.0	30.0	41.0
46	36.111915738412506	38.0	35.0	40.0	30.0	41.0
47	36.03802196428643	38.0	35.0	40.0	30.0	41.0
48	35.92015706632236	38.0	35.0	40.0	30.0	41.0
49	35.864991044347434	38.0	35.0	40.0	30.0	41.0
50	35.729282656883484	38.0	35.0	40.0	29.0	41.0
51	33.90283216694879	36.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	8.0
9	16.0
10	39.0
11	19.0
12	12.0
13	16.0
14	19.0
15	19.0
16	65.0
17	83.0
18	156.0
19	311.0
20	512.0
21	864.0
22	1289.0
23	2025.0
24	2879.0
25	3540.0
26	4648.0
27	5290.0
28	6438.0
29	7820.0
30	9653.0
31	12015.0
32	16077.0
33	22820.0
34	46072.0
35	69534.0
36	51823.0
37	82003.0
38	155646.0
39	250225.0
40	101.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.4762026969346	20.952104877539597	24.333046557425877	11.238645868099924
2	31.114875691287285	23.997824580906045	25.79985878391945	19.08744094388722
3	31.171122774217825	24.726776137939655	26.29837016431329	17.803730923529233
4	28.419403780920938	25.171300956466354	25.987349060354582	20.42194620225813
5	24.322940698554195	30.324624121887293	23.79544145981791	21.5569937197406
6	25.152020041513808	35.271575011402334	23.217146983068698	16.359257964015164
7	80.64900889448553	5.676567305684944	8.529211915871384	5.1452118839581455
8	80.68557614698175	4.244992613414996	8.566444027503893	6.502987212099373
9	75.11871059878543	5.5873432095941835	11.230002699328093	8.063943492292289
10	44.17816097303464	19.99896281974738	18.68852546211034	17.134350745107636
11	30.893876514382896	21.70206598328012	22.817965557637304	24.586091944699675
12	24.360039838359445	18.371520625924987	33.46754623098004	23.800893304735528
13	21.931708328956347	22.087817254158363	34.20061991465868	21.779854502226613
14	16.89207607584181	26.2636645173987	30.223033645861452	26.62122576089804
15	15.468346721380142	21.897800513005308	38.97005341478301	23.66379935083154
16	18.237485024047956	21.823070346085775	36.69198007018253	23.24746455968374
17	19.69911134927843	20.673794843086597	30.10322602950113	29.523867778133845
18	20.50598439708579	21.640234083604707	31.917493640622364	25.936287878687143
19	23.605956605973894	23.531093467227098	28.044556199877928	24.81839372692108
20	25.23180313786918	22.799881389130086	26.115400930004963	25.852914542995777
21	22.336075655650838	23.563272649423766	26.55886197391359	27.54178972101181
22	20.211584771534454	21.93370290636523	24.777571375952576	33.077140946147736
23	19.50111629848984	26.230953447892997	25.661568083570135	28.606362170047035
24	23.35211338773654	24.17095389999721	28.692394942283578	23.784537769982673
25	22.267063277303436	23.550374382179648	28.801697784290443	25.380864556226474
26	18.41939048373821	22.89602002023831	33.05227521445032	25.632314281573166
27	19.592202000162224	22.678744054497173	34.62267249437861	23.106381450961987
28	17.469040834318434	22.88870656973907	34.62187466341506	25.02037793252744
29	18.856867795420186	22.12265587290021	29.904034232267207	29.1164420994124
30	20.50212821409528	22.556941860727967	28.876294979382717	28.064634945794037
31	22.267728136439732	26.260872109026263	25.701991519056854	25.769408235477147
32	21.135738970984217	27.65867195717243	28.102797860217354	23.10279121162599
33	22.439660709085565	23.98572414462548	28.420068640057234	25.154546506231725
34	23.067021790093335	22.83737944441711	26.419773442600714	27.67582532288884
35	18.671372096393938	23.284962614970766	29.495677750754943	28.54798753788035
36	21.96441939846205	22.221055025071838	32.242742730097774	23.57178284636834
37	20.58510263430487	25.77579088318558	32.88473071210403	20.754375770405524
38	21.624277464333634	27.995888511101153	27.540991890048254	22.83884213451696
39	23.85115665543941	23.456629243962084	28.01051541209964	24.68169868849887
40	22.664516068980465	21.66430198433858	29.51416083474394	26.157021111937013
41	18.8629844994741	22.962505933867792	24.241295996617197	33.93321357004091
42	19.128662210337495	25.786827544848073	25.212389251089373	29.87212099372506
43	19.93779577920826	25.078220677385083	27.031709791646445	27.952273751760217
44	19.066165451525784	24.960407638433647	27.83672123387218	28.13670567616839
45	20.468220398144247	24.565614283301798	29.077614325852778	25.888550992701177
46	22.329825979769666	23.59983990191998	31.979192568470516	22.091141549839836
47	21.211532912521825	22.849745824352194	32.231041209298986	23.707680053826998
48	21.63371846406902	22.285147445810658	28.32845105107581	27.752683039044516
49	21.01194219980613	19.34221496491538	32.282235362793685	27.3636074724848
50	22.21945936314473	20.46875228545328	31.246650772100914	26.065137579301073
51	23.707813025654254	21.079757831708196	25.900651428981742	29.311777713655808
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	438.0
1	723.5
2	1009.0
3	1443.0
4	1877.0
5	1495.0
6	1113.0
7	1025.5
8	938.0
9	935.0
10	932.0
11	1036.5
12	1141.0
13	1104.5
14	1068.0
15	1150.5
16	1233.0
17	1237.0
18	1241.0
19	1276.5
20	1312.0
21	1328.0
22	1344.0
23	1548.0
24	1752.0
25	1907.5
26	2656.5
27	3250.0
28	4032.5
29	4815.0
30	5376.5
31	5938.0
32	6840.5
33	7743.0
34	9863.0
35	11983.0
36	13347.5
37	14712.0
38	16486.0
39	18260.0
40	22587.0
41	26914.0
42	34429.0
43	41944.0
44	54154.0
45	66364.0
46	75642.0
47	84920.0
48	86390.0
49	87860.0
50	76646.5
51	65433.0
52	63581.5
53	61730.0
54	56071.5
55	50413.0
56	46493.5
57	42574.0
58	37186.0
59	31798.0
60	29912.0
61	28026.0
62	25947.5
63	23869.0
64	21037.0
65	18205.0
66	15451.0
67	12697.0
68	10953.0
69	9209.0
70	7553.0
71	5897.0
72	4766.5
73	3636.0
74	3101.0
75	2195.0
76	1824.0
77	1424.5
78	1025.0
79	797.5
80	570.0
81	402.0
82	234.0
83	152.5
84	71.0
85	57.5
86	44.0
87	34.0
88	24.0
89	23.0
90	22.0
91	14.0
92	6.0
93	4.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	752039.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.374039856055326
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.18335741359134	18.51018665657834
2	12.256664698481933	6.465155265201377
3	5.07181481345258	4.0129273809759045
4	2.541803580485292	2.6815051575193287
5	1.4391996123790547	1.8978753968852284
6	0.9446111781247916	1.4947927716203178
7	0.6850800931462238	1.264783077686002
8	0.47908003748250005	1.0108220802243149
9	0.3808349669644935	0.9039740937570958
>10	4.4748192834908025	29.225549554508834
>50	1.4294921387620725	24.732172612901337
>100	0.1041015589505529	4.602125564937103
>500	0.004570312344170616	0.808405503461974
>1k	0.004570312344170616	2.389724883742877
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC	4297	0.5713799417317452	TruSeq Adapter, Index 15 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC	3737	0.49691571846672844	TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCT	2438	0.32418531485734114	TruSeq Adapter, Index 14 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC	1508	0.20052151550650965	TruSeq Adapter, Index 15 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCG	1235	0.16422020666481393	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGC	1226	0.1630234602194833	No Hit
GGCAAGGTGGGGTGAGGCCCGGAGTTGGGGAAGCTAGGAGGCTTAAAGCCT	1225	0.16289048839222434	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTC	1119	0.14879547470277474	No Hit
ACTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC	1058	0.14068419323997824	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	909	0.12087139097839342	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3297182725895864E-4	0.0	0.0	0.3671352150619848	0.0
2	1.3297182725895864E-4	0.0	0.0	1.9810142825039658	0.0
3	1.3297182725895864E-4	0.0	0.0	2.707572346646916	0.0
4	1.3297182725895864E-4	0.0	0.0	3.466176621159275	0.0
5	1.3297182725895864E-4	0.0	0.0	5.609283561091911	0.0
6	1.3297182725895864E-4	0.0	0.0	6.714279445613858	0.0
7	1.3297182725895864E-4	0.0	0.0	7.836694639506728	0.0
8	1.3297182725895864E-4	0.0	0.0	9.665456179799186	0.0
9	1.3297182725895864E-4	0.0	0.0	10.366616624935675	0.0
10	1.3297182725895864E-4	0.0	0.0	11.95456618606216	0.0
11	1.3297182725895864E-4	0.0	0.0	13.856462231347045	0.0
12	1.3297182725895864E-4	0.0	0.0	15.48137796045152	0.0
13	1.3297182725895864E-4	0.0	0.0	16.058741634409916	0.0
14	1.3297182725895864E-4	0.0	0.0	16.281203501414154	0.0
15	1.3297182725895864E-4	0.0	0.0	16.727191010040702	0.0
16	1.3297182725895864E-4	0.0	0.0	17.693231335077037	0.0
17	1.3297182725895864E-4	0.0	0.0	18.855538077147596	0.0
18	1.3297182725895864E-4	0.0	0.0	20.329530782313153	0.0
19	1.3297182725895864E-4	0.0	0.0	21.116458056031668	0.0
20	1.3297182725895864E-4	0.0	0.0	21.768418925082344	0.0
21	1.3297182725895864E-4	0.0	0.0	22.567845550563202	0.0
22	1.3297182725895864E-4	0.0	0.0	23.351581500427503	0.0
23	1.3297182725895864E-4	0.0	0.0	24.239966278344607	0.0
24	1.3297182725895864E-4	0.0	0.0	24.90243192174874	0.0
25	2.659436545179173E-4	0.0	0.0	25.433122484339243	0.0
26	2.659436545179173E-4	0.0	0.0	25.9289744281879	0.0
27	2.659436545179173E-4	0.0	0.0	26.41033244286533	0.0
28	2.659436545179173E-4	0.0	0.0	26.91841779482181	0.0
29	2.659436545179173E-4	0.0	0.0	27.51958342585956	1.3297182725895864E-4
30	2.659436545179173E-4	0.0	0.0	28.244811771729925	1.3297182725895864E-4
31	5.318873090358346E-4	0.0	0.0	28.78893248887358	1.3297182725895864E-4
32	5.318873090358346E-4	0.0	0.0	29.27720503856848	1.3297182725895864E-4
33	5.318873090358346E-4	0.0	0.0	29.777046137234905	1.3297182725895864E-4
34	5.318873090358346E-4	0.0	0.0	30.310795051852363	1.3297182725895864E-4
35	5.318873090358346E-4	0.0	0.0	30.901588880363917	1.3297182725895864E-4
36	5.318873090358346E-4	0.0	0.0	31.394648415840138	1.3297182725895864E-4
37	5.318873090358346E-4	0.0	0.0	31.95206631570969	1.3297182725895864E-4
38	5.318873090358346E-4	0.0	0.0	32.44778528773109	1.3297182725895864E-4
39	5.318873090358346E-4	0.0	0.0	32.934861090980654	1.3297182725895864E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTAC	20	7.031728E-4	45.000004	13
GTCGCAA	20	7.031728E-4	45.000004	14
TACCACG	20	7.031728E-4	45.000004	2
ATTAAGT	20	7.031728E-4	45.000004	13
CGACATT	20	7.031728E-4	45.000004	26
ACCCTTA	20	7.031728E-4	45.000004	21
GCCGATA	35	1.2111741E-7	45.000004	9
AGTGCCG	20	7.031728E-4	45.000004	10
CCCCGTA	20	7.031728E-4	45.000004	43
CGCTAGG	65	0.0	45.000004	2
ACGGACC	20	7.031728E-4	45.000004	21
TTGCGTA	20	7.031728E-4	45.000004	27
CGTACTT	20	7.031728E-4	45.000004	15
ATGACGG	70	0.0	45.000004	2
TACTCCG	20	7.031728E-4	45.000004	14
ATAACGC	40	6.8102963E-9	45.000004	11
TAGCTAA	20	7.031728E-4	45.000004	11
TAAGACG	20	7.031728E-4	45.000004	1
GCGTATG	75	0.0	45.000004	1
AGTACGG	85	0.0	45.000004	2
>>END_MODULE