FastQCFastQC Report
Sat 18 Jun 2016
SRR3549934_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549934_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2284925
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66470.2909067037211287No Hit
CCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC46440.20324518310229No Hit
GCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC37160.16263115857194438No Hit
CTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCT29190.12775036379749882Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTG26650.11663402518682232No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATAT3200.039.37514
ATAACGC1700.038.3823511
GCTACGA3350.037.61194210
TCTAGCG601.5643309E-1037.5000041
TACGGGA7900.037.025324
CGGTCTA2500.036.931
TATTACG552.752131E-936.818181
TCGTAAG552.752131E-936.818181
CGTTTTT31950.035.7746471
TTACGGG6050.035.7024773
ATGCGAA1450.035.68965526
CGTAAGG2800.035.3571432
TAGGGCG6850.035.145995
CGTTCGG7500.035.145
TCATGCG900.035.0000041
TAATGCG1500.034.524
GGGCGAT38450.034.3498047
TGTAACG1450.034.1379281
TACGAAT3700.034.05405412
TATCACG401.5612399E-533.751
Produced by FastQC (version 0.11.5)