##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549934_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2284925
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.63157784172347	34.0	31.0	34.0	31.0	34.0
2	32.81482718251146	34.0	31.0	34.0	31.0	34.0
3	32.78620873770474	34.0	31.0	34.0	31.0	34.0
4	36.211494031532766	37.0	37.0	37.0	35.0	37.0
5	36.22137269275797	37.0	37.0	37.0	35.0	37.0
6	36.217938006717944	37.0	37.0	37.0	35.0	37.0
7	36.30857117848507	37.0	37.0	37.0	35.0	37.0
8	36.29328402463976	37.0	37.0	37.0	35.0	37.0
9	37.68618882457849	39.0	38.0	39.0	35.0	39.0
10	37.69394794139851	39.0	37.0	39.0	35.0	39.0
11	37.66802105101918	39.0	37.0	39.0	35.0	39.0
12	37.572542205980504	39.0	37.0	39.0	35.0	39.0
13	37.55973959757979	39.0	37.0	39.0	35.0	39.0
14	38.862164403645636	40.0	38.0	41.0	35.0	41.0
15	38.89616026784249	40.0	38.0	41.0	35.0	41.0
16	38.90394783198573	40.0	38.0	41.0	35.0	41.0
17	38.85290414346204	40.0	38.0	41.0	35.0	41.0
18	38.786173725614624	40.0	38.0	41.0	35.0	41.0
19	38.746998260336774	40.0	38.0	41.0	35.0	41.0
20	38.70745910697288	40.0	38.0	41.0	35.0	41.0
21	38.67275468560237	40.0	38.0	41.0	35.0	41.0
22	38.63593247043119	40.0	38.0	41.0	35.0	41.0
23	38.5920242458724	40.0	38.0	41.0	35.0	41.0
24	38.555742967493465	40.0	38.0	41.0	35.0	41.0
25	38.47212928214274	40.0	38.0	41.0	35.0	41.0
26	38.38696324824666	40.0	38.0	41.0	34.0	41.0
27	38.29943477357025	40.0	37.0	41.0	34.0	41.0
28	38.21340087749051	40.0	37.0	41.0	34.0	41.0
29	38.12696740593236	40.0	37.0	41.0	34.0	41.0
30	37.99451010427038	40.0	37.0	41.0	34.0	41.0
31	37.88972985984223	40.0	37.0	41.0	34.0	41.0
32	37.79708349289364	40.0	36.0	41.0	33.0	41.0
33	37.646963029421094	40.0	36.0	41.0	33.0	41.0
34	37.54350011488342	40.0	36.0	41.0	33.0	41.0
35	37.46026849896605	40.0	36.0	41.0	33.0	41.0
36	37.305497992275455	40.0	35.0	41.0	32.0	41.0
37	37.21692221845356	40.0	35.0	41.0	32.0	41.0
38	37.08815081457816	40.0	35.0	41.0	32.0	41.0
39	36.98514962197884	39.0	35.0	41.0	31.0	41.0
40	36.86321170279112	39.0	35.0	41.0	31.0	41.0
41	36.74611683096819	39.0	35.0	41.0	31.0	41.0
42	36.61567272448767	39.0	35.0	41.0	31.0	41.0
43	36.53475059356434	39.0	35.0	41.0	31.0	41.0
44	36.4434342483889	39.0	35.0	41.0	31.0	41.0
45	36.30870509972975	39.0	35.0	41.0	30.0	41.0
46	36.16545444598838	38.0	35.0	41.0	30.0	41.0
47	36.05916080396512	38.0	35.0	40.0	30.0	41.0
48	35.914152543300105	38.0	35.0	40.0	29.0	41.0
49	35.80695777760758	38.0	35.0	40.0	29.0	41.0
50	35.69248837489196	38.0	35.0	40.0	29.0	41.0
51	33.970027024957055	36.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	6.0
8	28.0
9	76.0
10	88.0
11	68.0
12	51.0
13	56.0
14	65.0
15	94.0
16	188.0
17	365.0
18	566.0
19	1057.0
20	1844.0
21	2794.0
22	4210.0
23	6063.0
24	8549.0
25	11834.0
26	15231.0
27	18157.0
28	20960.0
29	23728.0
30	28371.0
31	35445.0
32	45170.0
33	63316.0
34	123542.0
35	164623.0
36	153690.0
37	246944.0
38	469579.0
39	837723.0
40	444.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.64038206943335	19.986389049968817	24.134971607383175	12.238257273214657
2	33.74421479917284	24.14486252283992	24.962132236287843	17.1487904416994
3	33.01955206407213	23.425933017495105	26.114292591660558	17.440222326772215
4	28.878190750243444	26.070439948794817	25.183933827149684	19.86743547381205
5	25.514491722923072	30.47636136853507	23.369563552414192	20.639583356127662
6	26.518813527796315	35.380154709673185	23.1392715297001	14.961760232830398
7	82.34953007210302	5.656772104117203	7.398273466306334	4.59542435747344
8	82.7493243760736	5.036532927776623	7.485891221812532	4.728251474337232
9	75.7936037287876	7.3973981640535245	9.927371795573158	6.88162631158572
10	38.96880641596551	26.76219131918991	17.240084466667398	17.028917798177183
11	30.334912524481112	25.130715450178894	23.57070801010974	20.96366401523026
12	27.288860684705185	22.214252108931365	29.291158353118814	21.205728853244636
13	25.04309769467269	22.6505465168441	30.419685547665676	21.886670240817534
14	21.622679081370286	26.654966793220787	29.043754171362295	22.678599954046632
15	20.79429302931168	25.49510377802335	32.08100922349749	21.62959396916748
16	23.257962515181024	23.47031084171253	30.63058962548005	22.6411370176264
17	23.058524896878456	23.34505508933554	27.937459654036783	25.658960359749226
18	23.869798789894638	24.5114391063164	28.38732124686806	23.23144085692091
19	24.29865312865849	25.42363534908148	27.162904690525945	23.114806831734082
20	26.29981290414346	23.97816120879241	26.533912491657276	23.18811339540685
21	25.759838944385482	25.2483123078438	26.87322340995875	22.118625337811963
22	23.37122662669453	23.65263630097268	26.643237743033144	26.332899329299646
23	23.532238476098776	25.299254898957297	26.555882578202784	24.612624046741143
24	23.845246561703338	24.21554317975426	27.879514644900816	24.059695613641587
25	23.61145332997801	24.99508736610611	26.48660240489294	24.906856899022944
26	22.77212599975929	25.551998424455945	26.38462969244067	25.291245883344093
27	23.193233913585782	25.54692167138965	27.66388393492128	23.595960480103287
28	22.48065034957384	24.890576277120694	28.24044552884668	24.38832784445879
29	24.3451754433953	24.621639659945075	26.51150475398536	24.521680142674267
30	24.64220926288609	23.899340240926943	28.2348435944287	23.223606901758266
31	25.172642428088448	25.12870225499743	25.75651279582481	23.942142521089313
32	25.69567928925457	25.23347593465869	25.773318599078742	23.297526177008
33	24.636826154031315	24.86729323719597	26.30248257601453	24.193398032758186
34	23.321815814523454	24.48456732715516	27.69009923739291	24.503517620928477
35	24.671444358129918	23.757059859732816	26.885915292624485	24.685580489512784
36	24.67853430637767	26.332811799074367	26.619079400855604	22.369574493692355
37	23.741041828506408	26.189130934275738	27.62025011761874	22.449577119599113
38	23.591846559515083	26.593170454172455	26.53137411512413	23.28360887118833
39	23.695044695121283	24.99723185662549	27.431885072814204	23.875838375439017
40	24.26342221298292	23.646509185203016	29.811656837751787	22.278411764062277
41	22.15477532085298	24.87696532708951	28.249417376937974	24.718841975119535
42	23.096950665776774	25.54280775080145	26.99952952503912	24.36071205838266
43	23.541429149753274	24.176723524842174	27.747694125627753	24.5341531997768
44	23.478845038677417	23.974134818429487	27.504185038896246	25.042835103996847
45	23.876801207917108	23.27384925106951	27.65447443570358	25.194875105309798
46	24.159129949560707	24.089543420462377	28.004245215926126	23.74708141405079
47	23.073011149162443	24.716916310163352	29.065986848583652	23.14408569209055
48	22.394826963685897	24.228322592645274	28.796393754718423	24.580456688950402
49	22.560346619692112	24.24604746326466	29.028874033064543	24.164731883978686
50	22.006499119227108	23.31927743799031	29.713185334310754	24.96103810847183
51	22.31871943280414	23.282821099160806	28.08998107158878	26.308478396446272
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	910.0
1	2220.5
2	3531.0
3	7524.0
4	11517.0
5	8145.0
6	4773.0
7	4339.0
8	3905.0
9	3825.0
10	3745.0
11	3895.5
12	4046.0
13	4147.0
14	4248.0
15	4203.0
16	4158.0
17	4146.0
18	4134.0
19	4607.5
20	5081.0
21	5631.5
22	6182.0
23	7059.5
24	7937.0
25	10226.5
26	14316.5
27	16117.0
28	20181.0
29	24245.0
30	27702.5
31	31160.0
32	35168.0
33	39176.0
34	44886.5
35	50597.0
36	54974.0
37	59351.0
38	65467.5
39	71584.0
40	79462.5
41	87341.0
42	96312.5
43	105284.0
44	119304.0
45	133324.0
46	151377.0
47	169430.0
48	188764.0
49	208098.0
50	205153.0
51	202208.0
52	184266.5
53	166325.0
54	151566.0
55	136807.0
56	130208.0
57	123609.0
58	121091.5
59	118574.0
60	116611.5
61	114649.0
62	105018.0
63	95387.0
64	87633.5
65	79880.0
66	67838.5
67	55797.0
68	47554.5
69	39312.0
70	34005.0
71	28698.0
72	24609.5
73	20521.0
74	16926.5
75	10760.0
76	8188.0
77	6295.0
78	4402.0
79	3340.0
80	2278.0
81	1843.5
82	1409.0
83	992.5
84	576.0
85	438.5
86	301.0
87	223.0
88	145.0
89	105.0
90	65.0
91	52.5
92	40.0
93	29.5
94	19.0
95	15.5
96	12.0
97	6.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2284925.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.298710730184904
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.5005324044618	17.61669464680414
2	10.260830776956896	4.986499178013081
3	3.87375196109223	2.8238153502919983
4	1.9605797626522956	1.9055824206457084
5	1.1561964934809814	1.4047042071174243
6	0.8066599987760871	1.1760478780722867
7	0.6058841924756755	1.030554331027068
8	0.4817515040265611	0.9364752352138332
9	0.3982949466407582	0.8710248324346396
>10	6.618733561260383	42.191963831543084
>50	1.242453737032338	18.657026231645197
>100	0.0854419400818511	3.7691855826369443
>500	0.006167684002273238	1.044747696888
>1k	0.0025396345891713326	1.2926890433986322
>5k	1.8140247065509521E-4	0.2929895342677638
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6647	0.2909067037211287	No Hit
CCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC	4644	0.20324518310229	No Hit
GCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC	3716	0.16263115857194438	No Hit
CTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCT	2919	0.12775036379749882	Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTG	2665	0.11663402518682232	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.625906758427519E-4	0.0	0.0	0.14455616705143495	0.0
2	2.625906758427519E-4	0.0	0.0	0.6773088832237382	0.0
3	2.625906758427519E-4	0.0	0.0	1.0343446721446	0.0
4	2.625906758427519E-4	0.0	0.0	1.3561495453898924	0.0
5	2.625906758427519E-4	0.0	0.0	2.275391971290086	0.0
6	2.625906758427519E-4	0.0	0.0	3.0207556046697377	0.0
7	2.625906758427519E-4	0.0	0.0	3.6301847981881243	0.0
8	2.625906758427519E-4	0.0	0.0	4.570434478155738	0.0
9	2.625906758427519E-4	0.0	0.0	4.989397901462849	0.0
10	2.625906758427519E-4	0.0	0.0	5.772618357276497	0.0
11	2.625906758427519E-4	0.0	0.0	6.779128417781766	0.0
12	2.625906758427519E-4	0.0	0.0	7.584056369465081	0.0
13	3.063557884832106E-4	0.0	0.0	7.916802520870488	0.0
14	3.063557884832106E-4	0.0	0.0	8.077770605162096	0.0
15	3.5012090112366927E-4	0.0	0.0	8.343906255128724	0.0
16	3.5012090112366927E-4	0.0	0.0	8.904712408503562	0.0
17	3.5012090112366927E-4	0.0	0.0	9.55619987526943	0.0
18	3.5012090112366927E-4	0.0	0.0	10.292416600107224	0.0
19	3.5012090112366927E-4	0.0	0.0	10.697550247819951	0.0
20	3.5012090112366927E-4	0.0	0.0	11.107585588148407	0.0
21	3.5012090112366927E-4	0.0	0.0	11.621956957011719	0.0
22	3.5012090112366927E-4	0.0	0.0	12.16324387014891	0.0
23	3.5012090112366927E-4	0.0	0.0	12.71000142236616	0.0
24	3.5012090112366927E-4	0.0	0.0	13.158156175804457	0.0
25	3.5012090112366927E-4	0.0	0.0	13.572174141383197	0.0
26	3.5012090112366927E-4	0.0	0.0	13.939450966661925	0.0
27	3.5012090112366927E-4	0.0	0.0	14.320076151295995	0.0
28	3.5012090112366927E-4	0.0	0.0	14.707703753952536	0.0
29	3.5012090112366927E-4	0.0	0.0	15.139752945939145	0.0
30	3.9388601376412794E-4	0.0	0.0	15.66703502303139	0.0
31	3.9388601376412794E-4	0.0	0.0	16.08774905084412	0.0
32	3.9388601376412794E-4	0.0	0.0	16.5096009715855	0.0
33	3.9388601376412794E-4	0.0	0.0	16.91486591463615	0.0
34	3.9388601376412794E-4	0.0	0.0	17.318380253181175	0.0
35	3.9388601376412794E-4	0.0	0.0	17.758263400330428	0.0
36	3.9388601376412794E-4	0.0	0.0	18.172981607711414	0.0
37	3.9388601376412794E-4	0.0	0.0	18.58958171493594	0.0
38	3.9388601376412794E-4	0.0	0.0	18.997866450758778	0.0
39	4.3765112640458656E-4	0.0	0.0	19.427596091775442	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATAT	320	0.0	39.375	14
ATAACGC	170	0.0	38.38235	11
GCTACGA	335	0.0	37.611942	10
TCTAGCG	60	1.5643309E-10	37.500004	1
TACGGGA	790	0.0	37.02532	4
CGGTCTA	250	0.0	36.9	31
TATTACG	55	2.752131E-9	36.81818	1
TCGTAAG	55	2.752131E-9	36.81818	1
CGTTTTT	3195	0.0	35.774647	1
TTACGGG	605	0.0	35.702477	3
ATGCGAA	145	0.0	35.689655	26
CGTAAGG	280	0.0	35.357143	2
TAGGGCG	685	0.0	35.14599	5
CGTTCGG	750	0.0	35.1	45
TCATGCG	90	0.0	35.000004	1
TAATGCG	150	0.0	34.5	24
GGGCGAT	3845	0.0	34.349804	7
TGTAACG	145	0.0	34.137928	1
TACGAAT	370	0.0	34.054054	12
TATCACG	40	1.5612399E-5	33.75	1
>>END_MODULE