FastQCFastQC Report
Sat 18 Jun 2016
SRR3549932_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549932_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences859824
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC53350.6204758183070024TruSeq Adapter, Index 19 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC48660.5659297716742031TruSeq Adapter, Index 19 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGCT31200.36286495841009325TruSeq Adapter, Index 13 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC18870.2194635181153352TruSeq Adapter, Index 19 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC14120.16421965425482424TruSeq Adapter, Index 13 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG11130.12944509574052365No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC10420.12118759187926831No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10340.12025716890898602No Hit
GCCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG9230.10734755019631925No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT8860.10304434395876365No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCGA253.889983E-545.024
AGCGTTG351.211447E-745.01
AGACTTA453.8380676E-1045.08
GGTCGAT207.0322945E-445.08
TAGCGTA207.0322945E-445.01
TAAACGG351.211447E-745.02
TCGATAG453.8380676E-1045.01
TCTACGA253.889983E-545.016
AATTCGT351.211447E-745.031
TCGTACA351.211447E-745.034
AATTAGC207.0322945E-445.019
ACCAACG207.0322945E-445.01
CCAACGG453.8380676E-1045.02
ATCGGTT207.0322945E-445.034
GCACTAT253.889983E-545.034
GTACGCG207.0322945E-445.01
AGCACGT253.889983E-545.02
ATTAGCG302.1648611E-644.9999961
CTATACG302.1648611E-644.9999961
TAGCACG302.1648611E-644.9999961
Produced by FastQC (version 0.11.5)