FastQCFastQC Report
Sat 18 Jun 2016
SRR3549930_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549930_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1087001
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC30540.2809565032598866TruSeq Adapter, Index 14 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC27020.24857382835894357TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGCT16330.15022985259443183TruSeq Adapter, Index 15 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13900.12787476736451944No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATAT600.045.00000414
ACGCGCC302.1653923E-645.00000422
ATTACGA207.033117E-445.036
ACCGAAC253.8906663E-545.011
TTGCGTC406.8139343E-945.016
TTACGAA207.033117E-445.037
CGTAAGG1550.042.096772
ACGCGTA603.6379788E-1241.25000425
TATTGCG1150.041.086961
GTATACG501.0822987E-940.51
CAATGCG451.9281288E-840.0000041
ACAACGA3450.039.78260813
ACGATAG403.4579898E-739.3751
ATGCGAA2300.039.13043626
TATGCGG1500.039.02
CGAAAGG1100.038.8636362
TCACGAC1050.038.5714325
CGATCAT356.2479776E-638.57142610
ATATGCG301.13988106E-437.5000041
AATGCGA2400.037.50000425
Produced by FastQC (version 0.11.5)