##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549930_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1087001
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.60998931923706	34.0	31.0	34.0	31.0	34.0
2	32.76235808430719	34.0	31.0	34.0	31.0	34.0
3	32.755106941023975	34.0	31.0	34.0	31.0	34.0
4	36.17231998866606	37.0	37.0	37.0	35.0	37.0
5	36.18521786088513	37.0	35.0	37.0	35.0	37.0
6	36.18367416405321	37.0	36.0	37.0	35.0	37.0
7	36.277675917501455	37.0	37.0	37.0	35.0	37.0
8	36.295116563830206	37.0	37.0	37.0	35.0	37.0
9	37.72374174448782	39.0	38.0	39.0	35.0	39.0
10	37.65247318079744	39.0	37.0	39.0	35.0	39.0
11	37.6281622556005	39.0	37.0	39.0	35.0	39.0
12	37.44029582309492	39.0	37.0	39.0	35.0	39.0
13	37.371104534402455	39.0	37.0	39.0	35.0	39.0
14	38.59454683114367	40.0	38.0	41.0	35.0	41.0
15	38.651294709020505	40.0	38.0	41.0	35.0	41.0
16	38.67984942056171	40.0	38.0	41.0	35.0	41.0
17	38.56431870807847	40.0	38.0	41.0	35.0	41.0
18	38.53594246923416	40.0	38.0	41.0	34.0	41.0
19	38.52950273274818	40.0	37.0	41.0	34.0	41.0
20	38.50220376982174	40.0	37.0	41.0	34.0	41.0
21	38.46883489527608	40.0	37.0	41.0	35.0	41.0
22	38.41725536591043	40.0	37.0	41.0	34.0	41.0
23	38.39009255741255	40.0	37.0	41.0	35.0	41.0
24	38.41213301551701	40.0	37.0	41.0	35.0	41.0
25	38.307581133780005	40.0	37.0	41.0	34.0	41.0
26	38.22607522900163	40.0	37.0	41.0	34.0	41.0
27	38.13311855278882	40.0	36.0	41.0	34.0	41.0
28	37.92517854169407	40.0	36.0	41.0	34.0	41.0
29	37.781337827656095	40.0	36.0	41.0	34.0	41.0
30	37.66726985531752	40.0	36.0	41.0	33.0	41.0
31	37.56162873815204	40.0	35.0	41.0	33.0	41.0
32	37.50761406843232	40.0	35.0	41.0	33.0	41.0
33	37.38282117495752	40.0	35.0	41.0	33.0	41.0
34	37.185896793103225	40.0	35.0	41.0	32.0	41.0
35	37.154824144596006	40.0	35.0	41.0	32.0	41.0
36	37.044275028265844	39.0	35.0	41.0	32.0	41.0
37	36.97634960777405	39.0	35.0	41.0	32.0	41.0
38	36.80480514737337	39.0	35.0	41.0	31.0	41.0
39	36.726474952644935	39.0	35.0	41.0	31.0	41.0
40	36.59998656854962	39.0	35.0	41.0	31.0	41.0
41	36.47590480597534	39.0	35.0	41.0	31.0	41.0
42	36.36414501918581	39.0	35.0	41.0	30.0	41.0
43	36.25569985676186	39.0	35.0	41.0	30.0	41.0
44	36.163378874536455	38.0	35.0	41.0	30.0	41.0
45	36.04528606689414	38.0	35.0	40.0	30.0	41.0
46	35.87625034383593	38.0	35.0	40.0	30.0	41.0
47	35.81367910425105	38.0	35.0	40.0	30.0	41.0
48	35.6928944867576	38.0	35.0	40.0	29.0	41.0
49	35.538805392083354	38.0	35.0	40.0	29.0	41.0
50	35.41060403808276	37.0	35.0	40.0	28.0	41.0
51	33.67168107481042	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	15.0
9	44.0
10	45.0
11	45.0
12	26.0
13	35.0
14	33.0
15	65.0
16	100.0
17	168.0
18	283.0
19	526.0
20	986.0
21	1735.0
22	2545.0
23	3563.0
24	4835.0
25	6269.0
26	7917.0
27	9147.0
28	10312.0
29	12147.0
30	14031.0
31	17451.0
32	22932.0
33	32730.0
34	70784.0
35	93221.0
36	72229.0
37	112669.0
38	218188.0
39	371737.0
40	184.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.743380180882994	19.026477436543296	23.75020814148285	11.479934241090854
2	32.28239900423275	23.713869628454802	25.443582848589834	18.560148518722613
3	32.97936248448713	23.961431498223092	25.314788118870172	17.744417898419595
4	28.616349018998143	26.26630518279192	25.068606192634597	20.04873960557534
5	24.850851103172857	29.825179553652664	24.414972939307322	20.908996403867153
6	25.971365251733896	34.99472401589327	23.518377627987462	15.515533104385367
7	83.0593532112666	5.497511041848167	6.960435178992476	4.482700567892762
8	83.81188241777147	4.422995011044148	7.057215218753249	4.707907352431138
9	77.32660779520903	6.5641153963979795	9.666596442873558	6.442680365519443
10	39.63023033097486	27.51267018153617	16.734667217417464	16.12243227007151
11	32.045508697784086	25.853150089098353	22.452325250850734	19.649015962266823
12	29.592061092860078	21.36630969060746	28.640544028938336	20.401085187594123
13	26.9723762903622	23.648368308768806	28.648823690134602	20.730431710734397
14	20.81258434904844	27.67127169156238	29.718831905398428	21.79731205399075
15	20.841011185822275	24.282038378989533	33.71892022178453	21.158030213403666
16	24.758118897774704	23.528497213894006	29.578353653768485	22.1350302345628
17	23.482039114959417	22.743677328723706	27.631069336642746	26.14321421967413
18	26.839993707457495	23.05572855958734	27.024630152134176	23.079647580820993
19	25.35379452272813	26.328494637999412	25.280381526787927	23.037329312484534
20	27.689394949958647	24.46520288389799	25.281853466556147	22.563548699587212
21	26.30908343230595	26.55912920043312	26.66142901432473	20.470358352936195
22	23.413317927030423	24.245055892312887	26.61690283633594	25.72472334432075
23	23.112398240663993	26.008347738410542	25.939166569303985	24.94008745162148
24	24.56695072037652	23.455452202895856	27.761887983543716	24.215709093183907
25	23.276335532350018	24.763454679434517	25.892892462840422	26.067317325375043
26	21.243034735018643	24.930151858185965	26.771548508235043	27.05526489856035
27	22.020862906289874	24.637143848073737	27.26740821765573	26.074585027980657
28	21.08728510829337	26.63677402320697	27.46354419177167	24.81239667672799
29	23.316813875976194	25.263546215688855	27.283875543812748	24.135764364522203
30	25.30144866472064	23.850392041957644	27.965567648971806	22.882591644349915
31	26.119019209733942	26.646985605349027	24.812948654141074	22.42104653077596
32	25.678817222799243	25.436315145984228	26.450021665113464	22.43484596610307
33	26.313315259139596	23.76317961069033	25.231623521965478	24.69188160820459
34	23.16925191421167	24.80218509458593	29.049007314620685	22.979555676581715
35	22.772012169262034	24.88157784583455	28.390222272104626	23.956187712798794
36	23.83668460286605	27.382863493225855	26.425274677760186	22.35517722614791
37	24.0289567350904	27.12325011660523	26.082772692941404	22.765020455362965
38	24.232268415576435	28.003562094239104	24.94983905258597	22.814330437598493
39	26.29611196309847	23.922701083071683	25.760141894993655	24.021045058836194
40	26.06464943454514	22.06134124991605	28.603745534732717	23.27026378080609
41	22.147265733886172	24.025460878140866	27.127481943438873	26.69979144453409
42	23.37219560975565	24.425552506391437	26.714234853509794	25.48801703034312
43	23.85738375585671	23.63098102025665	26.116627307610575	26.395007916276064
44	23.505866139957554	23.213226114787382	26.70301131277708	26.57789643247798
45	24.266123030245602	22.992343153318163	26.53815405873592	26.20337975770031
46	23.950299953725892	24.6565550537672	27.059128740451943	24.334016252054965
47	22.197035697299267	24.356095348578336	29.01276079782815	24.434108156294247
48	22.495195496600278	23.76023573115388	29.441739244030135	24.302829528215707
49	24.799885188698077	22.735581659998473	29.10576899193285	23.358764159370597
50	22.243677788704886	22.75140501250689	29.223432177155313	25.781485021632918
51	22.143585884465608	22.367504721706787	27.84744448257177	27.64146491125583
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	485.0
1	1098.0
2	1711.0
3	2404.0
4	3097.0
5	2394.0
6	1691.0
7	1627.5
8	1564.0
9	1581.0
10	1598.0
11	1742.5
12	1887.0
13	1841.5
14	1796.0
15	2009.5
16	2223.0
17	2103.5
18	1984.0
19	1994.5
20	2005.0
21	2345.0
22	2685.0
23	3160.0
24	3635.0
25	4114.5
26	5625.5
27	6657.0
28	7057.5
29	7458.0
30	9798.0
31	12138.0
32	14315.5
33	16493.0
34	18759.5
35	21026.0
36	23153.0
37	25280.0
38	26746.0
39	28212.0
40	32596.0
41	36980.0
42	43152.0
43	49324.0
44	57803.0
45	66282.0
46	75207.5
47	84133.0
48	92693.0
49	101253.0
50	102910.5
51	104568.0
52	95042.0
53	85516.0
54	78560.0
55	71604.0
56	68602.0
57	65600.0
58	63216.5
59	60833.0
60	57768.0
61	54703.0
62	51931.5
63	49160.0
64	42513.5
65	35867.0
66	29806.5
67	23746.0
68	20083.5
69	16421.0
70	13764.0
71	11107.0
72	9531.0
73	7955.0
74	6727.0
75	4545.0
76	3591.0
77	2784.0
78	1977.0
79	1592.5
80	1208.0
81	1052.0
82	896.0
83	582.5
84	269.0
85	207.0
86	145.0
87	107.5
88	70.0
89	49.0
90	28.0
91	26.5
92	25.0
93	20.0
94	15.0
95	10.5
96	6.0
97	3.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1087001.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.564633705976178
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.56906772946414	20.279044040528312
2	10.567545786133618	5.825810575318104
3	3.975419302338058	3.2874293068984777
4	1.9492193463957783	2.1491806918400767
5	1.1903311858026426	1.6405521562725056
6	0.7531650702841427	1.2456431569510917
7	0.5468289883690348	1.0551198534941366
8	0.43418889886091994	0.9574606365041914
9	0.32948736950771224	0.817397878610314
>10	5.55467886934461	39.56414506478199
>50	1.0516464389975464	18.002551225391148
>100	0.07339402639272441	3.6285177106275683
>500	0.0036864579466653782	0.7361607382058072
>1k	0.001340530162423774	0.810986964576331
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC	3054	0.2809565032598866	TruSeq Adapter, Index 14 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC	2702	0.24857382835894357	TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGCT	1633	0.15022985259443183	TruSeq Adapter, Index 15 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1390	0.12787476736451944	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.199623551404276E-5	0.0	0.0	0.1724929415888302	0.0
2	9.199623551404276E-5	0.0	0.0	0.9431454064899665	0.0
3	9.199623551404276E-5	0.0	0.0	1.3204219683330558	0.0
4	9.199623551404276E-5	0.0	0.0	1.7228135024714788	0.0
5	9.199623551404276E-5	0.0	0.0	2.7692706814437154	0.0
6	9.199623551404276E-5	0.0	0.0	3.4342194717392163	0.0
7	9.199623551404276E-5	0.0	0.0	4.062277771593586	0.0
8	9.199623551404276E-5	0.0	0.0	5.105515082322832	0.0
9	9.199623551404276E-5	0.0	0.0	5.5194061459005095	0.0
10	9.199623551404276E-5	0.0	0.0	6.431180836080188	0.0
11	1.8399247102808552E-4	0.0	0.0	7.686285477198273	0.0
12	1.8399247102808552E-4	0.0	0.0	8.732466667463967	0.0
13	1.8399247102808552E-4	0.0	0.0	9.27662440052953	0.0
14	1.8399247102808552E-4	0.0	0.0	9.53375387879128	0.0
15	1.8399247102808552E-4	0.0	0.0	9.86714823629417	0.0
16	1.8399247102808552E-4	0.0	0.0	10.543136574851356	0.0
17	1.8399247102808552E-4	0.0	0.0	11.367054860115124	0.0
18	1.8399247102808552E-4	0.0	0.0	12.370457800866788	0.0
19	1.8399247102808552E-4	0.0	0.0	12.89318041105758	0.0
20	1.8399247102808552E-4	0.0	0.0	13.430898407637159	0.0
21	1.8399247102808552E-4	0.0	0.0	14.052792959712088	0.0
22	1.8399247102808552E-4	0.0	0.0	14.682047210628141	0.0
23	1.8399247102808552E-4	0.0	0.0	15.289314361256338	0.0
24	1.8399247102808552E-4	0.0	0.0	15.786186029267682	0.0
25	1.8399247102808552E-4	0.0	0.0	16.23733556822855	0.0
26	1.8399247102808552E-4	0.0	0.0	16.657206387114638	0.0
27	2.759887065421283E-4	0.0	0.0	17.111483798082983	0.0
28	2.759887065421283E-4	0.0	0.0	17.540462244284964	0.0
29	2.759887065421283E-4	0.0	0.0	18.04625754714117	0.0
30	2.759887065421283E-4	0.0	0.0	18.678271685122645	0.0
31	3.6798494205617105E-4	0.0	0.0	19.138620847634915	0.0
32	3.6798494205617105E-4	0.0	0.0	19.62160108408364	0.0
33	3.6798494205617105E-4	0.0	0.0	20.11295297796414	0.0
34	3.6798494205617105E-4	0.0	0.0	20.626383968368014	0.0
35	3.6798494205617105E-4	0.0	0.0	21.140274939949457	0.0
36	3.6798494205617105E-4	0.0	0.0	21.591516475145838	0.0
37	3.6798494205617105E-4	0.0	0.0	22.091884000106717	0.0
38	3.6798494205617105E-4	0.0	0.0	22.610466779699376	0.0
39	3.6798494205617105E-4	0.0	0.0	23.21911387386028	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATAT	60	0.0	45.000004	14
ACGCGCC	30	2.1653923E-6	45.000004	22
ATTACGA	20	7.033117E-4	45.0	36
ACCGAAC	25	3.8906663E-5	45.0	11
TTGCGTC	40	6.8139343E-9	45.0	16
TTACGAA	20	7.033117E-4	45.0	37
CGTAAGG	155	0.0	42.09677	2
ACGCGTA	60	3.6379788E-12	41.250004	25
TATTGCG	115	0.0	41.08696	1
GTATACG	50	1.0822987E-9	40.5	1
CAATGCG	45	1.9281288E-8	40.000004	1
ACAACGA	345	0.0	39.782608	13
ACGATAG	40	3.4579898E-7	39.375	1
ATGCGAA	230	0.0	39.130436	26
TATGCGG	150	0.0	39.0	2
CGAAAGG	110	0.0	38.863636	2
TCACGAC	105	0.0	38.57143	25
CGATCAT	35	6.2479776E-6	38.571426	10
ATATGCG	30	1.13988106E-4	37.500004	1
AATGCGA	240	0.0	37.500004	25
>>END_MODULE