FastQCFastQC Report
Sat 18 Jun 2016
SRR3549926_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549926_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1362059
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC55880.4102612295062108Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC46150.338825263810158Illumina Single End Adapter 1 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGCT31800.23347006260374917Illumina Single End Adapter 1 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20280.14889222860390042No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT19250.14133014795981672No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC18720.13743898024975423No Hit
TCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC15370.11284386359181209Illumina Single End Adapter 1 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTG14900.10939320543383216Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATACG406.8175723E-945.01
ACGTTAC207.0337474E-445.025
ATTACGC207.0337474E-445.027
ATAGCGC207.0337474E-445.02
GCACGAT253.8911872E-545.09
CGCGATC207.0337474E-445.036
TCGCTTA207.0337474E-445.041
TACTCGT207.0337474E-445.033
CGTAACG207.0337474E-445.02
TACCTCG302.1657997E-644.9999961
GCCGATT1100.042.9545449
GTACGAG1000.042.751
CGTAAGG1600.042.18752
CGACGGT1150.041.08695628
CGGTCTA1150.041.08695631
GAATGCG556.184564E-1140.9090921
TCGTAAG556.184564E-1140.9090921
AATGCGA3600.040.62525
AGGGTAA4100.040.0609746
CGAGGGT2700.040.0000044