##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549926_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1362059
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.591080856262465	34.0	31.0	34.0	31.0	34.0
2	32.7678624787913	34.0	31.0	34.0	31.0	34.0
3	32.76671348304295	34.0	31.0	34.0	31.0	34.0
4	36.17948414863086	37.0	37.0	37.0	35.0	37.0
5	36.19875790989965	37.0	35.0	37.0	35.0	37.0
6	36.19659280545116	37.0	36.0	37.0	35.0	37.0
7	36.31030740959092	37.0	37.0	37.0	35.0	37.0
8	36.29862729881745	37.0	37.0	37.0	35.0	37.0
9	37.738935684871215	39.0	38.0	39.0	35.0	39.0
10	37.68978289486726	39.0	37.0	39.0	35.0	39.0
11	37.66477076250001	39.0	37.0	39.0	35.0	39.0
12	37.55734149548588	39.0	37.0	39.0	35.0	39.0
13	37.51274724516339	39.0	37.0	39.0	35.0	39.0
14	38.791271156388966	40.0	38.0	41.0	35.0	41.0
15	38.824158865364865	40.0	38.0	41.0	35.0	41.0
16	38.84442817822136	40.0	38.0	41.0	35.0	41.0
17	38.78556802605467	40.0	38.0	41.0	35.0	41.0
18	38.74266826914253	40.0	38.0	41.0	35.0	41.0
19	38.713985223841256	40.0	38.0	41.0	35.0	41.0
20	38.68440647578409	40.0	38.0	41.0	35.0	41.0
21	38.66175180370307	40.0	38.0	41.0	35.0	41.0
22	38.6234157257505	40.0	38.0	41.0	35.0	41.0
23	38.60354580822123	40.0	38.0	41.0	35.0	41.0
24	38.59841020102653	40.0	38.0	41.0	35.0	41.0
25	38.52222040308092	40.0	38.0	41.0	35.0	41.0
26	38.42520404769544	40.0	37.0	41.0	35.0	41.0
27	38.32972507064672	40.0	37.0	41.0	34.0	41.0
28	38.24825136062388	40.0	37.0	41.0	34.0	41.0
29	38.16594288499984	40.0	37.0	41.0	34.0	41.0
30	38.04024568686085	40.0	37.0	41.0	34.0	41.0
31	37.9194197901853	40.0	37.0	41.0	34.0	41.0
32	37.824225675980266	40.0	36.0	41.0	34.0	41.0
33	37.68047566221434	40.0	36.0	41.0	33.0	41.0
34	37.57648897734973	40.0	36.0	41.0	33.0	41.0
35	37.50435260146587	40.0	36.0	41.0	33.0	41.0
36	37.37067777533866	40.0	35.0	41.0	33.0	41.0
37	37.238325212050285	40.0	35.0	41.0	32.0	41.0
38	37.13907473905316	40.0	35.0	41.0	32.0	41.0
39	37.061184574236506	39.0	35.0	41.0	32.0	41.0
40	36.96970028464259	39.0	35.0	41.0	32.0	41.0
41	36.870109885107766	39.0	35.0	41.0	31.0	41.0
42	36.74995723386432	39.0	35.0	41.0	31.0	41.0
43	36.67036449962887	39.0	35.0	41.0	31.0	41.0
44	36.57805205207704	39.0	35.0	41.0	31.0	41.0
45	36.456477289162954	39.0	35.0	41.0	31.0	41.0
46	36.32156609955956	39.0	35.0	41.0	31.0	41.0
47	36.2333724163197	39.0	35.0	41.0	30.0	41.0
48	36.1307542477969	38.0	35.0	40.0	30.0	41.0
49	36.04487837898358	38.0	35.0	40.0	30.0	41.0
50	35.91480325007947	38.0	35.0	40.0	30.0	41.0
51	34.22687710297425	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	20.0
9	51.0
10	57.0
11	42.0
12	24.0
13	24.0
14	45.0
15	78.0
16	101.0
17	176.0
18	319.0
19	537.0
20	994.0
21	1598.0
22	2385.0
23	3658.0
24	5318.0
25	6975.0
26	8517.0
27	9854.0
28	11315.0
29	13330.0
30	16333.0
31	20015.0
32	26424.0
33	37972.0
34	75639.0
35	102495.0
36	88154.0
37	142185.0
38	278826.0
39	508289.0
40	308.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.44613559324523	20.02695918458745	24.871242728839206	11.655662493328116
2	31.473820150228445	25.046712367085423	25.663645994777024	17.815821487909115
3	31.670067155681213	24.40136587328449	26.890905606878995	17.037661364155294
4	28.639948783422746	26.402747604912857	25.384216102239332	19.57308750942507
5	24.542108675174866	31.13073662741482	23.906820482813153	20.420334214597165
6	25.272840603821127	35.37967151202701	23.983102053582115	15.364385830569748
7	81.92523231372503	5.800262690529558	7.492553553113337	4.7819514426320735
8	81.91421957492297	5.268053733355163	7.4233935534363775	5.394333138285493
9	75.7280705167691	6.9905929185152775	10.226208996820255	7.0551275678953695
10	38.04211124481392	26.541948623370942	18.922234646223107	16.493705485592034
11	28.584444579860346	26.379547435169844	23.729515388099927	21.306492596869887
12	25.848733424910375	22.291104864033056	31.11722766781762	20.74293404323895
13	24.72704926879085	24.926306422849525	30.079166908335104	20.26747740002452
14	20.22019604143433	28.279465133301862	28.67394143719178	22.826397388072028
15	19.07142054786173	25.964807691884122	33.74817096763063	21.21560079262352
16	21.665581300075843	24.125460057163455	30.209998245303616	23.998960397457086
17	21.339016885465316	24.22464812464071	28.13071974121532	26.305615248678656
18	22.223560066047064	25.36358557155013	29.664060073755984	22.74879428864682
19	22.971471867224547	27.822583309533584	26.414714781077762	22.791230042164106
20	24.91727597703183	25.737945272561614	27.038549725085332	22.306229025321223
21	23.770482776443604	26.9884050544066	27.740134605035465	21.50097756411433
22	21.151726907571554	24.32647924943046	27.404980254159327	27.11681358883866
23	20.802109159735373	26.817487348198572	26.567645013909093	25.812758478156965
24	22.910681549037157	24.843710881834046	29.220320118291497	23.025287450837297
25	22.75921968137944	26.506487604428298	26.115461958696358	24.618830755495907
26	21.7107335291643	26.606556691009715	26.583943867336142	25.09876591248984
27	21.92834524789308	26.121849347201554	27.976688234503793	23.973117170401576
28	21.08968847898659	25.46651797022009	28.516385854063593	24.92740769672973
29	22.91985883137221	24.482052539574276	27.682868363264735	24.91522026578878
30	25.200817292055632	24.602605320327534	28.121175367586865	22.07540202002997
31	25.81804459278196	27.06600815383181	24.689532538605157	22.426414714781078
32	25.39075032726189	26.456269515490888	25.35323359707619	22.799746560171037
33	24.59489640316609	26.448634016588123	25.745066843653614	23.211402736592174
34	22.85246086990358	25.70439312834466	27.656437790139783	23.78670821161198
35	24.110262477616608	24.339400862958215	27.209173758258636	24.341162901166545
36	25.672089094525276	27.02981295230236	25.75945682235498	21.53864113081739
37	22.79901237758423	28.13585901932295	28.499940164119174	20.565188438973642
38	23.377474837727295	27.904224413186213	26.715582805150145	22.002717943936347
39	23.180493649687715	26.71492204082202	26.639521489157225	23.465062820333042
40	24.74907474639498	24.810232155875774	28.6461159171519	21.794577180577345
41	22.02173327293458	25.866720898287078	27.012045733701694	25.099500095076642
42	22.800921252309923	25.88536913599191	27.19632556298956	24.117384048708608
43	23.2022988725158	23.955423370059595	28.90366716860283	23.938610588821778
44	23.58451432720609	23.90608630022635	27.88352046423833	24.625878908329227
45	23.516308764891978	24.177146511274476	27.186707771102427	25.119836952731124
46	24.78005725155812	24.803918185629257	27.54381418132401	22.872210381488614
47	21.98216083150583	25.81385975203717	29.819853618675847	22.384125797781156
48	22.1465443126913	24.491743749720094	29.2653255108626	24.096386426726006
49	22.837483545132773	23.635540016988983	30.430326439603572	23.09664999827467
50	21.859625757768203	23.227187662208465	30.404923722100147	24.50826285792319
51	22.55849416214716	22.83153666617966	27.57266755698542	27.037301614687763
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	705.0
1	1508.5
2	2312.0
3	3280.5
4	4249.0
5	3255.0
6	2261.0
7	2238.0
8	2215.0
9	2442.0
10	2669.0
11	2759.5
12	2850.0
13	2820.0
14	2790.0
15	2720.0
16	2650.0
17	2508.0
18	2366.0
19	2479.5
20	2593.0
21	2892.0
22	3191.0
23	3424.5
24	3658.0
25	5588.5
26	8810.5
27	10102.0
28	13096.0
29	16090.0
30	17961.0
31	19832.0
32	23595.5
33	27359.0
34	32092.0
35	36825.0
36	39771.0
37	42717.0
38	44759.0
39	46801.0
40	51279.0
41	55757.0
42	61434.5
43	67112.0
44	77074.0
45	87036.0
46	101497.0
47	115958.0
48	129737.5
49	143517.0
50	139044.5
51	134572.0
52	115861.5
53	97151.0
54	86810.0
55	76469.0
56	70604.0
57	64739.0
58	62279.0
59	59819.0
60	55212.5
61	50606.0
62	46521.5
63	42437.0
64	40103.0
65	37769.0
66	32295.0
67	26821.0
68	22773.0
69	18725.0
70	16720.0
71	14715.0
72	12685.5
73	10656.0
74	8630.0
75	5418.0
76	4232.0
77	3437.5
78	2643.0
79	1881.5
80	1120.0
81	1014.5
82	909.0
83	634.5
84	360.0
85	307.5
86	255.0
87	172.0
88	89.0
89	88.5
90	88.0
91	78.5
92	69.0
93	58.5
94	48.0
95	34.0
96	20.0
97	13.0
98	6.0
99	4.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1362059.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.31311624565263
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.66437261423863	17.88735478410334
2	11.528393635508653	5.836391364425449
3	4.669317444458143	3.545849257782679
4	2.3779079518833566	2.4076904162994057
5	1.402653472243978	1.775276519764005
6	0.8958091214222428	1.360543225468631
7	0.6551645761026977	1.160897995244492
8	0.5126082363854062	1.0380569500882213
9	0.39407280266561895	0.8977689596812447
>10	5.493692293417075	35.72209318501775
>50	1.305163481355059	21.030951621590706
>100	0.09151772335516833	4.404267412370818
>500	0.006412069787781283	1.1319609846338405
>1k	0.0026231194586377976	1.3886312750295342
>5k	2.91457717626422E-4	0.4122660484998958
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC	5588	0.4102612295062108	Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC	4615	0.338825263810158	Illumina Single End Adapter 1 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGCT	3180	0.23347006260374917	Illumina Single End Adapter 1 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2028	0.14889222860390042	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1925	0.14133014795981672	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	1872	0.13743898024975423	No Hit
TCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC	1537	0.11284386359181209	Illumina Single End Adapter 1 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTG	1490	0.10939320543383216	Illumina Single End Adapter 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.202547760412728E-4	0.0	0.0	0.25725757841620667	0.0
2	2.202547760412728E-4	0.0	0.0	1.3088272974959234	0.0
3	2.202547760412728E-4	0.0	0.0	1.8041068705540655	0.0
4	2.202547760412728E-4	0.0	0.0	2.326477781065284	0.0
5	2.202547760412728E-4	0.0	0.0	3.7257563732554906	0.0
6	2.202547760412728E-4	0.0	0.0	4.582474033797361	0.0
7	2.202547760412728E-4	0.0	0.0	5.386110293313285	0.0
8	2.202547760412728E-4	0.0	0.0	6.789793981024317	0.0
9	2.202547760412728E-4	0.0	0.0	7.323104212078919	0.0
10	2.202547760412728E-4	0.0	0.0	8.44618331511337	0.0
11	2.202547760412728E-4	0.0	0.0	9.822408574077922	0.0
12	2.202547760412728E-4	0.0	0.0	10.9468826240273	0.0
13	2.202547760412728E-4	0.0	0.0	11.375057908651534	0.0
14	2.202547760412728E-4	0.0	0.0	11.536431241231107	0.0
15	2.202547760412728E-4	0.0	0.0	11.891775613244361	0.0
16	2.202547760412728E-4	0.0	0.0	12.616193571644107	0.0
17	2.202547760412728E-4	0.0	0.0	13.537959809376833	0.0
18	2.202547760412728E-4	0.0	0.0	14.65700090818386	0.0
19	2.202547760412728E-4	0.0	0.0	15.187888336702008	0.0
20	2.202547760412728E-4	0.0	0.0	15.720978312980568	0.0
21	2.202547760412728E-4	0.0	0.0	16.374474233495025	0.0
22	2.202547760412728E-4	0.0	0.0	17.115704973132587	0.0
23	2.202547760412728E-4	0.0	0.0	17.87859409908088	0.0
24	2.202547760412728E-4	0.0	0.0	18.487231463541594	0.0
25	2.202547760412728E-4	0.0	0.0	19.001673202115327	0.0
26	2.202547760412728E-4	0.0	0.0	19.442770100267317	0.0
27	2.202547760412728E-4	0.0	0.0	19.909416552440092	0.0
28	2.202547760412728E-4	0.0	0.0	20.38487319565452	0.0
29	2.202547760412728E-4	0.0	0.0	20.956728012516344	0.0
30	2.202547760412728E-4	0.0	0.0	21.675052255445618	0.0
31	4.405095520825456E-4	0.0	0.0	22.16196214701419	0.0
32	4.405095520825456E-4	0.0	0.0	22.657608811365733	0.0
33	4.405095520825456E-4	0.0	0.0	23.151273182732908	0.0
34	4.405095520825456E-4	0.0	0.0	23.649416067879585	0.0
35	4.405095520825456E-4	0.0	0.0	24.21099232852615	0.0
36	4.405095520825456E-4	0.0	0.0	24.684246423980166	0.0
37	4.405095520825456E-4	0.0	0.0	25.188996952408083	0.0
38	4.405095520825456E-4	0.0	0.0	25.68001826646276	0.0
39	4.405095520825456E-4	0.0	0.0	26.21310824274132	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGATACG	40	6.8175723E-9	45.0	1
ACGTTAC	20	7.0337474E-4	45.0	25
ATTACGC	20	7.0337474E-4	45.0	27
ATAGCGC	20	7.0337474E-4	45.0	2
GCACGAT	25	3.8911872E-5	45.0	9
CGCGATC	20	7.0337474E-4	45.0	36
TCGCTTA	20	7.0337474E-4	45.0	41
TACTCGT	20	7.0337474E-4	45.0	33
CGTAACG	20	7.0337474E-4	45.0	2
TACCTCG	30	2.1657997E-6	44.999996	1
GCCGATT	110	0.0	42.954544	9
GTACGAG	100	0.0	42.75	1
CGTAAGG	160	0.0	42.1875	2
CGACGGT	115	0.0	41.086956	28
CGGTCTA	115	0.0	41.086956	31
GAATGCG	55	6.184564E-11	40.909092	1
TCGTAAG	55	6.184564E-11	40.909092	1
AATGCGA	360	0.0	40.625	25
AGGGTAA	410	0.0	40.060974	6
CGAGGGT	270	0.0	40.000004	4
>>END_MODULE