SRR3549925_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549925_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1493048
Sequences flagged as poor quality	0
Sequence length	51
%GC	50

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	5304	0.3552464488750529	TruSeq Adapter, Index 15 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	4921	0.3295942260396183	TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCT	3405	0.22805696802781958	TruSeq Adapter, Index 14 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2027	0.13576254748675193	No Hit
TCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	1821	0.12196526836377665	TruSeq Adapter, Index 15 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG	1544	0.1034126163391934	TruSeq Adapter, Index 14 (95% over 21bp)
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	1520	0.10180516634428363	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTAACG	35	1.2122473E-7	45.000004	1
ACTATCG	20	7.033966E-4	45.0	22
TCTAGCG	55	1.8189894E-12	45.0	1
CTACGTC	20	7.033966E-4	45.0	34
ATAAGCG	55	1.8189894E-12	45.0	1
TACTCGT	25	3.891368E-5	45.0	39
TATACGG	45	3.8562575E-10	45.0	2
CGACGGT	100	0.0	42.75	28
GCCGATA	90	0.0	42.5	9
CGTTGAT	45	1.9290383E-8	40.0	25
CGGTCTA	110	0.0	38.863636	31
ATAGCGG	195	0.0	38.076923	2
CGTTCGG	415	0.0	37.95181	45
TACGAAG	90	0.0	37.5	1
TTATCCG	30	1.1400855E-4	37.499996	1
ATCGCGA	30	1.1400855E-4	37.499996	38
ATGCGAA	325	0.0	37.384617	26
CGAATAT	200	0.0	37.125	14
CACAACG	195	0.0	36.923077	12
AATGCGA	330	0.0	36.818184	25